P47288 (POTA_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Spermidine/putrescine import ATP-binding protein PotA
EC=3.6.3.31

Gene names

Name:	potA
Ordered Locus Names:	MG042

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Part of the ABC transporter complex PotABCD involved in spermidine/putrescine import. Responsible for energy coupling to the transport system By similarity. HAMAP MF_01726
Catalytic activity	ATP + H2O + polyamine(Out) = ADP + phosphate + polyamine(In). HAMAP MF_01726
Subunit structure	The complex is composed of two ATP-binding proteins (PotA), two transmembrane proteins (PotB and PotC) and a solute-binding protein (PotD) Potential.
Subcellular location	Cell membrane; Peripheral membrane protein By similarity HAMAP MF_01726.
Sequence similarities	Belongs to the ABC transporter superfamily. Spermidine/putrescine importer (TC 3.A.1.11.1) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	ATP-binding cassette (ABC) transporter complex Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW polyamine-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Spermidine/putrescine import ATP-binding protein PotA HAMAP MF_01726		PRO_0000092750
Regions
	Domain	7 – 448	442	ABC transporter
	Nucleotide binding	40 – 47	8	ATP Potential
	Region	108 – 317	210	Insert HAMAP MF_01726

Sequences

	Sequence		Length	Mass (Da)
	P47288 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: DB8F04B26A110F6F	FASTA	559	65,192
	10 20 30 40 50 60 MNEHSLIEIE GLNKTFDDGY VSIRDISLNI KKGEFITILG PSGCGKTTLL RLLAGFEDPT 70 80 90 100 110 120 YGKIKVNGID IKDMAIHKRP FATVFQDYAL FSHLTVYKNI AYGLKVMWTK LDEIPKLVSD 130 140 150 160 170 180 YQKQLALKHL KLERKIEQLQ KNNSNAQRIK KLKEKLQKLL EINKQKVIEF ENKEKLRRED 190 200 210 220 230 240 IYKNLEQLTK EWDLLSQKKL KEVEQQKQAI DKSFEKVENK YKKDPWFFQH SEIRLKQYQK 250 260 270 280 290 300 KKTELKADIK ATKNKEQIQK LTKELQTLKQ KYANKKAIDK EYDKLVVAYN KKDYWTSYWE 310 320 330 340 350 360 TYTLQQKEAF EKRYLSRKLT KAEQNKKVSD VIEMVGLKGK EDRLPDELSG GMKQRVALAR 370 380 390 400 410 420 SLVVEPEILL LDEPLSALDA KVRKNLQKEL QQIHKKSGLT FILVTHDQEE ALVLSDRIVV 430 440 450 460 470 480 MNEGNILQVG NPVDIYDSPK TEWIANFIGQ ANIFKGTYLG EKKIQLQSGE IIQTDVDNNY 490 500 510 520 530 540 VVGKQYKILI RPEDFDLVPE NKGFFNVRVI DKNYKGLLWK ITTQLKDNTI VDLESVNEVD 550 VNKTFGVLFD PIDVHLMEV

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71258.1.
RefSeq	NP_072702.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47288.
SMR	P47288. Positions 30-65.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875517.
GenomeReviews	Gene locus MG042 in contig L43967_GR.
KEGG	mge:MG_042.
PATRIC	20009462. VBIMycGen98045_0041.
TIGR	MG042.
Phylogenomic databases
HOGENOM	HBG758042.
OMA	KTEWIAN.
PhylomeDB	P47288.
ProtClustDB	CLSK542114.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_042-MONOMER.
Family and domain databases
HAMAP	MF_01726. ABC_ATPase_PotA. [Tree]
InterPro	IPR017879. ABC_Sperm/Put_ATP-bd_PotA. IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR003593. ATPase_AAA+_core. IPR008995. Mo/tungstate-bd. [Graphical view]
KO	K11072.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
SUPFAM	SSF50331. MOP_like. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51305. POTA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

POTA_MYCGE

Accession

Primary (citable) accession number: P47288

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program