P47284 (GLPK_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycerol kinase
EC=2.7.1.30

Alternative name(s):
ATP:glycerol 3-phosphotransferase
Glycerokinase
Short name=GK

Gene names

Name:	glpK
Ordered Locus Names:	MG038

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	508 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Key enzyme in the regulation of glycerol uptake and metabolism. HAMAP MF_00186
Catalytic activity	ATP + glycerol = ADP + sn-glycerol 3-phosphate. HAMAP MF_00186
Pathway	Polyol metabolism; glycerol degradation via glycerol kinase pathway; sn-glycerol 3-phosphate from glycerol: step 1/1. HAMAP MF_00186
Sequence similarities	Belongs to the FGGY kinase family.

Ontologies

Keywords
Biological process	Glycerol metabolism
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol-3-phosphate metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glycerol kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 508	508	Glycerol kinase HAMAP MF_00186		PRO_0000059467
Regions
	Nucleotide binding	419 – 423	5	ATP By similarity
Sites
	Binding site	15	1	Substrate By similarity
	Binding site	19	1	ATP By similarity
	Binding site	85	1	Substrate By similarity
	Binding site	138	1	Substrate By similarity
	Binding site	251	1	Substrate By similarity
	Binding site	273	1	ATP By similarity
	Binding site	317	1	ATP; via carbonyl oxygen By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47284 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 82033D7076D27CB5	FASTA	508	56,902
	10 20 30 40 50 60 MDLKKQYIIA LDEGTSSCRS IVFDHNLNQI AIAQNEFNTF FPNSGWVEQD PLEIWSAQLA 70 80 90 100 110 120 TMQSAKNKAQ IKSHEVIAVG ITNQRETIVL WNKENGLPVY NAIVWQDQRT AALCQKFNED 130 140 150 160 170 180 KLIQTKVKQK TGLPINPYFS ATKIAWILKN VPLAKKLMEQ KKLLFGTIDS WLIWKLTNGK 190 200 210 220 230 240 MHVTDVSNAS RTLLFDIVKM EWSKELCDLF EVPVSILPKV LSSNAYFGDI ETNHWSSNAK 250 260 270 280 290 300 GIVPIRAVLG DQQAALFGQL CTEPGMVKNT YGTGCFVLMN IGDKPTLSKH NLLTTVAWQL 310 320 330 340 350 360 ENHPPVYALE GSVFVAGAAI KWLRDALKII YSEKESDFYA ELAKENEQNL VFVPAFSGLG 370 380 390 400 410 420 APWWDASARG IILGIEASTK REHIVKASLE SIAFQTNDLL NAMASDLGYK ITSIKADGGI 430 440 450 460 470 480 VKSNYLMQFQ ADIADVIVSI PKNKETTAVG VCFLAGLACG FWKDIHQLEK LTTLDKKFKS 490 500 TMDPNIRKTK INSWHKAVER ALKWKEID

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71254.1.
PIR	B64204.
RefSeq	NP_072698.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47284.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875413.
GenomeReviews	Gene locus MG038 in contig L43967_GR.
KEGG	mge:MG_038.
PATRIC	20009452. VBIMycGen98045_0036.
TIGR	MG038.
Phylogenomic databases
HOGENOM	HBG511469.
OMA	EGAIFIT.
PhylomeDB	P47284.
ProtClustDB	PRK00047.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_038-MONOMER.
Family and domain databases
HAMAP	MF_00186. Glycerol_kin. [Tree]
InterPro	IPR000577. Carb_kinase_FGGY. IPR018485. Carb_kinase_FGGY_C. IPR018483. Carb_kinase_FGGY_CS. IPR018484. Carb_kinase_FGGY_N. IPR005999. Glycerol_kin. [Graphical view]
KO	K00864.
PANTHER	PTHR10196. FGGY_kin. 1 hit. PTHR10196:SF9. Glycerol_kin. 1 hit.
Pfam	PF02782. FGGY_C. 1 hit. PF00370. FGGY_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR01311. Glycerol_kin. 1 hit.
PROSITE	PS00933. FGGY_KINASES_1. 1 hit. PS00445. FGGY_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPK_MYCGE

Accession

Primary (citable) accession number: P47284

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program