Reviewed, UniProtKB/Swiss-Prot P47282 (SYD_MYCGE)

Names and origin

Protein names

Recommended name:
    Aspartyl-tRNA synthetase
    EC=6.1.1.12
Alternative name(s):
    Aspartate--tRNA ligase
      Short name=AspRS

Gene names

Name:	aspS
Ordered Locus Names:	MG036

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	550 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-aspartate + tRNA(Asp) = AMP + diphosphate + L-aspartyl-tRNA(Asp). HAMAP MF_00044
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aspartyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP aspartate-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 550	550	Aspartyl-tRNA synthetase HAMAP MF_00044		PRO_0000110902
Experimental info
	Sequence conflict	374 – 377	4	LGAI → CWSN Ref.2
	Sequence conflict	469 – 470	2	MN → II Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P47282-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 4B7972691E38D4BA	FASTA	550	64,237
	10 20 30 40 50 60 MCFNQRILIG SISTEQLNKT IVIIGWIKRI KKLGEINFII VGDKSGTIQV TCKDKEQIQQ 70 80 90 100 110 120 LTREDIVIVK AKLQRLDSVR FELINPTIKL FSKSKTPPLI IEDETDALEE VRLKYRYLDL 130 140 150 160 170 180 RRRLMQKRLL LRHQFILAIR NWFNQQGFIE IETPTLSKST PEGAQDFLVP ARIRKDCFYA 190 200 210 220 230 240 LVQSPQIYKQ LLMIAGVEKY FQIARVYRDE DSRKDRQPEH TQIDFEISFC NQKMIMNLVE 250 260 270 280 290 300 KLFFSVFLDV FQIKIKKTFP VFKFSELFER FGSDKPDLRY GFEIKDFTSL FQDHQNQFTK 310 320 330 340 350 360 LIEAKGIIGG IELTNIELST DKIKALRKIA KDHDVSLEVH NKNNSTLKTS IKCDEKNTLL 370 380 390 400 410 420 LVANKSKKKA WTALGAIRNE LKYHLDIVKP NQYSFCWVVD FPLYDFDEKT NQWISNHNIF 430 440 450 460 470 480 SKPKQEWIDN FESNKNEALS EQFDLVLNGF EIGSGSIRIN DPIVQKRLMN SLNIDPNKFA 490 500 510 520 530 540 FLLEAYQYGA PVHGGMGLGI DRLMMILNQT DNIREVIAFP KNNHGIEVHT NAPDKIDKEE 550 VKWWIKELVK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 374-550. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium." Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III Nucleic Acids Res. 19:6027-6031(1991) [PubMed: 1945886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 388-470. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71252.1. U01814 Genomic DNA. Translation: AAD12351.1. X61523 Genomic DNA. Translation: CAA43736.1.
PIR	I64203.
RefSeq	NP_072696.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875228.
GenomeReviews	Gene locus MG036 in contig L43967_GR.
KEGG	mge:MG_036.
TIGR	MG036.
Phylogenomic databases
HOGENOM	P47282.
OMA	PLFEYDE
Enzyme and pathway databases
BioCyc	MGEN243273:MG_036-MON.
BRENDA	6.1.1.12. 110.
Family and domain databases
HAMAP	MF_00044. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II_cons-reg. IPR002312. Asp-tRNA-synth_IIb. IPR020564. Asp-tRNA-synth_IIb_bac-type. IPR004524. Asp-tRNA-synth_IIb_bac/mt. IPR018153. Asp-tRNA-synth_IIb_C_bac/mt. IPR016027. NA-bd_OB-fold-like. IPR004365. NA_bd_OB_tRNA-helicase. [Graphical view]
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF5. AspS_bac. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
TIGRFAMs	TIGR00459. aspS_bact. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYD_MYCGE

Accession

Primary (citable) accession number: P47282
Secondary accession number(s): Q49462, Q49479

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)