Reviewed, UniProtKB/Swiss-Prot P47281 (SYH_MYCGE)

Names and origin

Protein names

Recommended name:
    Histidyl-tRNA synthetase
    EC=6.1.1.21
Alternative name(s):
    Histidine--tRNA ligase
      Short name=HisRS

Gene names

Name:	hisS
Ordered Locus Names:	MG035

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	414 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His). HAMAP MF_00127
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	histidyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP histidine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 414	414	Histidyl-tRNA synthetase HAMAP MF_00127		PRO_0000136198

Sequences

	Sequence		Length	Mass (Da)
	P47281-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 9419FD5BDB12110A	FASTA	414	48,324
	10 20 30 40 50 60 MNFLQKPRGV KDWFGDELVY FNWIVKKIRS LAFNWGFSEV KTPLFENAQL FQRSNANADI 70 80 90 100 110 120 VQKELYQFFD KSQRELALRP EATTPIVRLA CENKLMQEAN FPLKLFCIGS MYRYERPQNN 130 140 150 160 170 180 RFREHWQFSC EVFGFSNLFI FLDTLLFANS LLEALGITGY VLKINNLANF ETLSKWNKAL 190 200 210 220 230 240 KDYLTPYKLE LTELSQKRLE KNPLRILDDK IDQKKSFVKN APKITDFLDA SAKQDSELLK 250 260 270 280 290 300 TQLKKHNISF EWTDNLVRGL DYYTGFVFEY VKNQDTILAG GVYDNLVEEL SSNPTPALGF 310 320 330 340 350 360 ACGIERLINC LEIDKKAFIL NTKPKQMLVI CLFEEALEEL VWLAKLWREY NQVTIYPKVI 370 380 390 400 410 KVDNGIRLAN RLGYTFIGIV GKTDFDKKAI TIKNLVSKQQ TIYTWNELGE RNVF

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71251.1.
PIR	H64203.
RefSeq	NP_072695.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875525.
GenomeReviews	Gene locus MG035 in contig L43967_GR.
KEGG	mge:MG_035.
TIGR	MG035.
Phylogenomic databases
HOGENOM	P47281.
OMA	VFEWVTT
Enzyme and pathway databases
BioCyc	MGEN243273:MG_035-MON.
BRENDA	6.1.1.21. 110.
Family and domain databases
HAMAP	MF_00127. [Tree]
InterPro	IPR002314. aa-tRNA-synt_IIb_cons-reg. IPR006195. aa-tRNA-synth_II_cons-reg. IPR004154. Anticodon_bd. IPR015807. His-tRNA-synth_IIa_sub. IPR004516. His-tRNA_synth_IIA. [Graphical view]
Gene3D	G3DSA:3.40.50.800. Anticodon_bd. 1 hit.
PANTHER	PTHR11476. His-tRNA_synth. 1 hit.
Pfam	PF00587. tRNA-synt_2b. 1 hit. [Graphical view]
PIRSF	PIRSF001549. His-tRNA_synth. 1 hit.
TIGRFAMs	TIGR00442. hisS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYH_MYCGE

Accession

Primary (citable) accession number: P47281

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)