P47280 (KITH_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thymidine kinase
EC=2.7.1.21

Gene names

Name:	tdk
Ordered Locus Names:	MG034

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Tenericutes › Mollicutes › Mycoplasmatales › Mycoplasmataceae › Mycoplasma › Mycoplasma genitalium

Protein attributes

Sequence length	213 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + thymidine = ADP + thymidine 5'-phosphate. HAMAP-Rule MF_00124
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_00124.
Sequence similarities	Belongs to the thymidine kinase family.

Ontologies

Keywords
Biological process	DNA synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: HAMAP thymidine kinase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 213	213	Thymidine kinase HAMAP-Rule MF_00124		PRO_0000174994
Regions
	Nucleotide binding	20 – 27	8	ATP By similarity
	Nucleotide binding	93 – 96	4	ATP By similarity
Sites
	Active site	94	1	Proton acceptor Potential
	Metal binding	150	1	Zinc By similarity
	Metal binding	153	1	Zinc By similarity
	Metal binding	185	1	Zinc By similarity
	Metal binding	188	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47280 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 08E9C93CE4556326	FASTA	213	24,101
	10 20 30 40 50 60 MGKYQPSFQT KKGWTEVICG PMFSGKTEKL LHKIKRWKIA KISVVIFKPI IDTRQTNIVK 70 80 90 100 110 120 SRNGEYDQAI TINSPFEIYD HLVDKNYQIV AIDEAQFFSN EIIEVVTTLN EIGTNVIISG 130 140 150 160 170 180 LDTDFRAEPF GCIPQLLAIA DVVNKLDAIC NVCGSLAQRT QRLVNKNTND NLVLIGDAEA 190 200 210 YEARCKLHHS FLTKKHVTVK TKNFKEQVQG KTQ

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71250.1.
PIR	G64203.
RefSeq	NP_072694.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47280.
ModBase	Search...
Protein-protein interaction databases
STRING	243273.MG_034.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC71250; AAC71250; MG_034.
GeneID	875417.
KEGG	mge:MG_034.
PATRIC	20009444. VBIMycGen98045_0032.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1435.
KO	K00857.
OMA	ELICGSM.
ProtClustDB	PRK04296.
Enzyme and pathway databases
BioCyc	MGEN243273:GH2R-73-MONOMER.
Family and domain databases
HAMAP	MF_00124. Thymidine_kinase.
InterPro	IPR001267. Thymidine_kinase. IPR020633. Thymidine_kinase_CS. IPR020634. Thymidine_kinase_subgr. [Graphical view]
PANTHER	PTHR11441. PTHR11441. 1 hit.
Pfam	PF00265. TK. 1 hit. [Graphical view]
PIRSF	PIRSF035805. TK_cell. 1 hit.
PROSITE	PS00603. TK_CELLULAR_TYPE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KITH_MYCGE

Accession

Primary (citable) accession number: P47280

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program