P47279 (GLPF_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Probable glycerol uptake facilitator protein

Gene names

Name:	glpF
Ordered Locus Names:	MG033

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	258 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane By similarity.
Subcellular location	Cell membrane; Multi-pass membrane protein Potential.
Domain	Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Sequence similarities	Belongs to the MIP/aquaporin (TC 1.A.8) family. [View classification]

Ontologies

Keywords
Biological process	Glycerol metabolism Transport
Cellular component	Cell membrane Membrane
Domain	Repeat Transmembrane Transmembrane helix
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycerol metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 258	258	Probable glycerol uptake facilitator protein		PRO_0000064087
Regions
	Transmembrane	11 – 31	21	Helical; Potential
	Transmembrane	50 – 70	21	Helical; Potential
	Transmembrane	95 – 115	21	Helical; Potential
	Transmembrane	152 – 172	21	Helical; Potential
	Transmembrane	180 – 200	21	Helical; Potential
	Transmembrane	233 – 253	21	Helical; Potential
	Motif	77 – 79	3	NPA 1
	Motif	206 – 208	3	NPA 2

Sequences

	Sequence		Length	Mass (Da)
	P47279 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 238DBE6ABA4E1438	FASTA	258	27,667
	10 20 30 40 50 60 MYFQNSTQLG WWFLAELIGT FILIIFGNGA VAQVNLKKMA TSETKAKFLT VALTWGIGVL 70 80 90 100 110 120 FGVLTANAIF KGSGHLNPAI SLFYAINGSI KSPTALIWPG FVIGILAQFL GAMIAQTTLN 130 140 150 160 170 180 FLFWKQLSST DPQTVLAMHC TSPSVFNITR NFLTEFIATL ILIGGVVAAS HFLHNNPNSV 190 200 210 220 230 240 PPGFMGLWLV AGIIIAFGGA TGSAINPARD LGTRIVFQLT PIKNKDANWK YSWIPVIAPL 250 SAGLVLSIII GFSPAPVL

References

[1]

"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71249.1.
RefSeq	NP_072693.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47279.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875476.
GenomeReviews	Gene locus MG033 in contig L43967_GR.
KEGG	mge:MG_033.
PATRIC	20009442. VBIMycGen98045_0031.
TIGR	MG033.
Phylogenomic databases
HOGENOM	HBG705794.
OMA	LPIAHKG.
PhylomeDB	P47279.
ProtClustDB	CLSK542109.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_033-MONOMER.
Family and domain databases
InterPro	IPR023271. Aquaporin-like. IPR000425. MIP. IPR022357. MIP_CS. [Graphical view]
Gene3D	G3DSA:1.20.1080.10. MIP. 1 hit.
KO	K02440.
PANTHER	PTHR19139. MIP. 1 hit.
Pfam	PF00230. MIP. 1 hit. [Graphical view]
PRINTS	PR00783. MINTRINSICP.
SUPFAM	SSF81338. MIP. 1 hit.
PROSITE	PS00221. MIP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GLPF_MYCGE

Accession

Primary (citable) accession number: P47279

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 1, 1997
Last modified:	January 25, 2012
This is version 77 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program