Reviewed, UniProtKB/Swiss-Prot P47277 (DPO3_MYCGE)

Names and origin

Protein names

Recommended name:
    DNA polymerase III polC-type
      Short name=PolIII
    EC=2.7.7.7

Gene names

Name:	polC
Ordered Locus Names:	MG031

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	1451 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for replicative DNA synthesis. This DNA polymerase also exhibits 3' to 5' exonuclease activity By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_00356
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DNA polymerase type-C family. PolC subfamily. Contains 1 exonuclease domain.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Exonuclease Hydrolase Nuclease Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA-dependent DNA replication Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: HAMAP DNA binding Inferred from electronic annotation. Source: HAMAP DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1451	1451	DNA polymerase III polC-type HAMAP MF_00356		PRO_0000204582
Regions
	Domain	416 – 575	160	Exonuclease

Sequences

	Sequence		Length	Mass (Da)
	P47277-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 2DB9E6951F41AAEF	FASTA	1,451	167,500
	10 20 30 40 50 60 MVFNLENEKD KKVLALSNFL IDNNILDHNE LNSLIERIES IYFNKYIEEK VFLFAITISR 70 80 90 100 110 120 PLTIDIWNAL YEGFNELNEG FKADNESFKL TITFKENEPF FKSKNSFSSV TIAIIKDYFH 130 140 150 160 170 180 SFFSKDKKYK ILIEQELSNP NFLSYENDEL KAQCQTKELT EWLVQKSKSF IFWMNNAGFK 190 200 210 220 230 240 NFNFIALYPI DKNESKLKVK AVTVSQYDKQ FETKVFATEF IPIHKINQQI DDVKIIGQIF 250 260 270 280 290 300 ELKTHESLTG KKTLNIYVTD FQLGGSLILK WSYTDEKKIE GITIGNWIKA HIQVERDPNT 310 320 330 340 350 360 QILYGIVREI NPVEIPNNYK RLDLSKQKRV ELVFHTKMTA FDGINDIEEY AQFAKERGWK 370 380 390 400 410 420 AITVTDKDNI HIYPKFYEVA KKYDLKAIYG LEFNLTDDHI KIVHNPDNTK LSDATFVIFD 430 440 450 460 470 480 IETTGLHGRY DDVIEFSARK IKNNSEIDHQ QFFLKIDKPI PKTITEITKI TDEMLEGGID 490 500 510 520 530 540 QQQGLEKIRN YLDDCVMVAH NGINFDLPFL QTQFEKYNIK PLTNPLIDTL CLSWALNPLF 550 560 570 580 590 600 SSHTLSNICS KLKLEFDDER LHRAEYDTEA LKKVFFYFKK QLKEMGINTL TEIDQNLNKK 610 620 630 640 650 660 CQIDLMKRVF TNTAIVYVKN QRGFQNLYEM LSIALTDHNA NRPLVLASSL AKFRKSFLLT 670 680 690 700 710 720 ENPVQGDIFK AALTKPINEL EKAIEKVDFV LISQPNAYLG YTLREGLKKE LINDAIKLVI 730 740 750 760 770 780 KTATKLKKLV AVASDAYFIH PWENEYYKAI VCAKGLGGKW HRHFNNKEKE QTVPEVFLHT 790 800 810 820 830 840 TDEMLKRMSF LGEDIAYKLV VENTNKIVKL LDLNELVPTK NKLYPPVMQD SNQKLIDKTW 850 860 870 880 890 900 KQAEKRYGKN LPKLIKERIE KELNAIISNG FGIVFWISHL LVEQSVKDGY FVGPRGSIGS 910 920 930 940 950 960 SLIANLIGIS EINPLAAHYL CEQCHYFEVS DSVDDGYDLM IRDCPKCHEK ASFKGDGHNI 970 980 990 1000 1010 1020 PFATFMGFSG DKIPDIDLNF SSEYQAKAHD YVRKLFGVNN TFRAGTIATV AEKTAYGYAR 1030 1040 1050 1060 1070 1080 NYFEIIKRVD LATTAEIERF KQKLIGIKRT TGQHPGGIMI FPSDHSVYEF TPCGFPADDV 1090 1100 1110 1120 1130 1140 ESEWKTTHFE YDALGDAILK LDILGQDDPT MLKHLADLTK INPQNIPHFD KNLISMFSSN 1150 1160 1170 1180 1190 1200 KPLNLKPGIV DEVTGAVGIP EFGTKFVRKI LEQTKPKDFA DLIRVSGLSH GKNVWADNAQ 1210 1220 1230 1240 1250 1260 KLIKSNRLTL RDVIACRDDI MLYLINKGMQ AKDAFEIMEK VRKGIKVNAK EVSLMQNCGV 1270 1280 1290 1300 1310 1320 EQYWINACLK INYLFPKAHA AAYVLMAWRI AWFKLYHPLS YYACLLSFKL KEHDINGFEK 1330 1340 1350 1360 1370 1380 GYEFIKNRLD ELNKLYRIKK IKPKEAELLT SYEVYLEMMA RNIKLQQISI QNSNARMFVE 1390 1400 1410 1420 1430 1440 HNGVLIAPFI TIPGMGEAVA SSIVEARNEK PFASLNDFKK RTKITKKHVE TMEQLQLFDE 1450 FEHQDDHKLF N

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 492-567; 975-1103 AND 1111-1225. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71247.1. U01807 Genomic DNA. Translation: AAD12336.1. U01712 Unassigned DNA. Translation: AAC43185.1. U02208 Genomic DNA. Translation: AAD12500.1.
PIR	D64203.
RefSeq	NP_072691.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875300.
GenomeReviews	Gene locus MG031 in contig L43967_GR.
KEGG	mge:MG_031.
TIGR	MG031.
Phylogenomic databases
HOGENOM	P47277.
OMA	WKTTHFD
Enzyme and pathway databases
BioCyc	MGEN243273:MG_031-MON.
BRENDA	2.7.7.7. 110.
Family and domain databases
HAMAP	MF_00356. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR006054. DnaQ. IPR006055. Exonuclease. IPR013520. Exonuclease_RNaseT/DNA_pol3. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR006308. PolC_gram_pos. IPR012337. PolynucTfrase_RNaseH_fold. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF00929. Exonuc_X-T. 1 hit. PF02811. PHP. 1 hit. [Graphical view]
SMART	SM00479. EXOIII. 1 hit. SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00573. dnaq. 1 hit. TIGR01405. polC_Gram_pos. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3_MYCGE

Accession

Primary (citable) accession number: P47277

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)