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P47267

- SYM_MYCGE

UniProt

P47267 - SYM_MYCGE

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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi126 – 1261ZincBy similarity
Metal bindingi129 – 1291ZincBy similarity
Metal bindingi143 – 1431ZincBy similarity
Metal bindingi147 – 1471ZincBy similarity
Binding sitei304 – 3041ATPBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
  3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:GH2R-21-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Synonyms:metS
Ordered Locus Names:MG021
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
ProteomesiUP000000807: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 512512Methionine--tRNA ligasePRO_0000139228Add
BLAST

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi243273.MG_021.

Structurei

3D structure databases

ProteinModelPortaliP47267.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi11 – 2111"HIGH" regionAdd
BLAST
Motifi301 – 3055"KMSKS" region

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0143.
KOiK01874.
OMAiSHAHWTM.
OrthoDBiEOG6CVV9B.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPiMF_01228. Met_tRNA_synth_type2.
InterProiIPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
PfamiPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSiPR01041. TRNASYNTHMET.
SUPFAMiSSF47323. SSF47323. 1 hit.
TIGRFAMsiTIGR00398. metG. 1 hit.

Sequencei

Sequence statusi: Complete.

P47267-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKRCYITTPI YYASGKPHIG HAFTTILADV IKRFKIQNGY EAFLLVGSDE
60 70 80 90 100
HGNKIESKAK SLNLDPKTFV DINAQAFKLM WKTLNISFDH FIRTTDEIHK
110 120 130 140 150
QQVQKTFQDL YDKKLIYQSE WKGAYCVECE QNYFTFNKQT MLCEIGHNLS
160 170 180 190 200
LVQEPCWFIS FSSTKNWIET TIGKNQLNII PKSRASELKN NFINNGLNDL
210 220 230 240 250
ALTRKNVTWG IKVPFDPNQT IYVWFDALFS YITNLGFRNG DPNFIKWWNN
260 270 280 290 300
DNKEREVIHL ISREITRFHC IYWPIFLHLL DIKLPTQFLS HGWIVDGEGR
310 320 330 340 350
KMSKSLNNVI SPEQLIDQFG VDGTRYCLLK EMRLDKDNRC SVSILKEIYN
360 370 380 390 400
ADLANSFGNH VSRTFGMIKK YLNGKLEYQI ITDNALQKIM ILIDESIVQF
410 420 430 440 450
DHYFNSYEFY RAINLLLKIV FELSKLIDDF KPWELFKNQE FSLLKQLLFT
460 470 480 490 500
CVRCVQVCYV LLTPILVNTA SKVFHLFNFA DDACRKDQLR DATLLKKIII
510
SNSMEVLFKR VD
Length:512
Mass (Da):60,007
Last modified:February 1, 1996 - v1
Checksum:iCA05EC9B4356F432
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti506 – 5083VLF → FYL(PubMed:1945886)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71237.1.
U02229 Genomic DNA. Translation: AAA03382.2.
X61513 Genomic DNA. Translation: CAA43727.1.
PIRiC64202.
RefSeqiNP_072681.1. NC_000908.2.

Genome annotation databases

EnsemblBacteriaiAAC71237; AAC71237; MG_021.
GeneIDi875451.
KEGGimge:MG_021.
PATRICi20009418. VBIMycGen98045_0019.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA. Translation: AAC71237.1 .
U02229 Genomic DNA. Translation: AAA03382.2 .
X61513 Genomic DNA. Translation: CAA43727.1 .
PIRi C64202.
RefSeqi NP_072681.1. NC_000908.2.

3D structure databases

ProteinModelPortali P47267.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 243273.MG_021.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC71237 ; AAC71237 ; MG_021 .
GeneIDi 875451.
KEGGi mge:MG_021.
PATRICi 20009418. VBIMycGen98045_0019.

Phylogenomic databases

eggNOGi COG0143.
KOi K01874.
OMAi SHAHWTM.
OrthoDBi EOG6CVV9B.

Enzyme and pathway databases

BioCyci MGEN243273:GH2R-21-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 1 hit.
HAMAPi MF_01228. Met_tRNA_synth_type2.
InterProi IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR015413. Methionyl/Leucyl_tRNA_Synth.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view ]
Pfami PF09334. tRNA-synt_1g. 1 hit.
[Graphical view ]
PRINTSi PR01041. TRNASYNTHMET.
SUPFAMi SSF47323. SSF47323. 1 hit.
TIGRFAMsi TIGR00398. metG. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
    Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
    J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
    Strain: ATCC 33530 / G-37 / NCTC 10195.
  3. "A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium."
    Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III
    Nucleic Acids Res. 19:6027-6031(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-508.
    Strain: ATCC 33530 / G-37 / NCTC 10195.

Entry informationi

Entry nameiSYM_MYCGE
AccessioniPrimary (citable) accession number: P47267
Secondary accession number(s): Q49339, Q49473
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. Mycoplasma genitalium
    Mycoplasma genitalium (strain G-37): entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3