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P47267 (SYM_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Methionine--tRNA ligase
EC=6.1.1.10
Alternative name(s):
Methionyl-tRNA synthetase
Short name=MetRS
Gene names
Name:metG
Synonyms:metS
Ordered Locus Names:MG021
OrganismMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]
Taxonomic identifier243273 [NCBI]
Taxonomic lineageBacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length512 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity. HAMAP MF_01228

Catalytic activity

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228

Cofactor

Binds 1 zinc ion per subunit By similarity. HAMAP MF_01228

Subunit structure

Monomer By similarity. HAMAP MF_01228

Subcellular location

Cytoplasm HAMAP MF_01228.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Metal-binding
Nucleotide-binding
Zinc
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processmethionyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

methionine-tRNA ligase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 512512Methionine--tRNA ligase HAMAP MF_01228
PRO_0000139228

Regions

Motif11 – 2111"HIGH" region HAMAP MF_01228
Motif301 – 3055"KMSKS" region HAMAP MF_01228

Sites

Metal binding1261Zinc By similarity
Metal binding1291Zinc By similarity
Metal binding1431Zinc By similarity
Metal binding1471Zinc By similarity
Binding site3041ATP By similarity

Experimental info

Sequence conflict506 – 5083VLF → FYL Ref.3

Sequences

Sequence LengthMass (Da)Tools
P47267 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: CA05EC9B4356F432

FASTA51260,007
        10         20         30         40         50         60 
MKRCYITTPI YYASGKPHIG HAFTTILADV IKRFKIQNGY EAFLLVGSDE HGNKIESKAK 

        70         80         90        100        110        120 
SLNLDPKTFV DINAQAFKLM WKTLNISFDH FIRTTDEIHK QQVQKTFQDL YDKKLIYQSE 

       130        140        150        160        170        180 
WKGAYCVECE QNYFTFNKQT MLCEIGHNLS LVQEPCWFIS FSSTKNWIET TIGKNQLNII 

       190        200        210        220        230        240 
PKSRASELKN NFINNGLNDL ALTRKNVTWG IKVPFDPNQT IYVWFDALFS YITNLGFRNG 

       250        260        270        280        290        300 
DPNFIKWWNN DNKEREVIHL ISREITRFHC IYWPIFLHLL DIKLPTQFLS HGWIVDGEGR 

       310        320        330        340        350        360 
KMSKSLNNVI SPEQLIDQFG VDGTRYCLLK EMRLDKDNRC SVSILKEIYN ADLANSFGNH 

       370        380        390        400        410        420 
VSRTFGMIKK YLNGKLEYQI ITDNALQKIM ILIDESIVQF DHYFNSYEFY RAINLLLKIV 

       430        440        450        460        470        480 
FELSKLIDDF KPWELFKNQE FSLLKQLLFT CVRCVQVCYV LLTPILVNTA SKVFHLFNFA 

       490        500        510 
DDACRKDQLR DATLLKKIII SNSMEVLFKR VD

« Hide

References

« Hide 'large scale' references
[1]"The minimal gene complement of Mycoplasma genitalium."
Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. expand/collapse author list , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C.
Science 270:397-403(1995) [PubMed: 7569993] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]"A survey of the Mycoplasma genitalium genome by using random sequencing."
Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium."
Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III
Nucleic Acids Res. 19:6027-6031(1991) [PubMed: 1945886] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-508.
Strain: ATCC 33530 / G-37 / NCTC 10195.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L43967 Genomic DNA. Translation: AAC71237.1.
U02229 Genomic DNA. Translation: AAA03382.2.
X61513 Genomic DNA. Translation: CAA43727.1.
PIRC64202.
RefSeqNP_072681.1. NC_000908.2.

3D structure databases

ProteinModelPortalP47267.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID875451.
GenomeReviewsGene locus MG021 in contig L43967_GR.
KEGGmge:MG_021.
PATRIC20009418. VBIMycGen98045_0019.
TIGRMG021.

Phylogenomic databases

HOGENOMHBG721667.
OMAFWGVLKS.
PhylomeDBP47267.
ProtClustDBPRK11893.

Enzyme and pathway databases

BioCycMGEN243273:MG_021-MONOMER.

Family and domain databases

HAMAPMF_01228. Met_tRNA_synth_type2.
[Tree]
InterProIPR015413. aa-tRNA-synt_I.
IPR014758. Met-tRNA_synth.
IPR023457. Met-tRNA_synth_2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
[Graphical view]
Gene3DG3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KOK01874.
PfamPF09334. tRNA-synt_1g. 1 hit.
[Graphical view]
PRINTSPR01041. TRNASYNTHMET.
SUPFAMSSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMsTIGR00398. MetG. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. False negative.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYM_MYCGE
AccessionPrimary (citable) accession number: P47267
Secondary accession number(s): Q49339, Q49473
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

SIMILARITY comments

Index of protein domains and families

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