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Protein

Methionine--tRNA ligase

Gene

metG

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

Catalytic activityi

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

Cofactori

Zn2+By similarityNote: Binds 1 zinc ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi126ZincBy similarity1
Metal bindingi129ZincBy similarity1
Metal bindingi143ZincBy similarity1
Metal bindingi147ZincBy similarity1
Binding sitei304ATPBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciMGEN243273:G1GJ2-21-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Methionine--tRNA ligase (EC:6.1.1.10)
Alternative name(s):
Methionyl-tRNA synthetase
Short name:
MetRS
Gene namesi
Name:metG
Synonyms:metS
Ordered Locus Names:MG021
OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Taxonomic identifieri243273 [NCBI]
Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
Proteomesi
  • UP000000807 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001392281 – 512Methionine--tRNA ligaseAdd BLAST512

Proteomic databases

PRIDEiP47267

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

STRINGi243273.MgenG_010200002776

Structurei

3D structure databases

ProteinModelPortaliP47267
SMRiP47267
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi11 – 21"HIGH" regionAdd BLAST11
Motifi301 – 305"KMSKS" region5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105CKH Bacteria
COG0143 LUCA
KOiK01874
OMAiTYVWFDA
OrthoDBiPOG091H0269

Family and domain databases

CDDicd00814 MetRS_core, 1 hit
Gene3Di3.40.50.620, 2 hits
HAMAPiMF_01228 Met_tRNA_synth_type2, 1 hit
InterProiView protein in InterPro
IPR014758 Met-tRNA_synth
IPR023457 Met-tRNA_synth_2
IPR015413 Methionyl/Leucyl_tRNA_Synth
IPR033911 MetRS_core
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
PfamiView protein in Pfam
PF09334 tRNA-synt_1g, 2 hits
PRINTSiPR01041 TRNASYNTHMET
SUPFAMiSSF47323 SSF47323, 1 hit
TIGRFAMsiTIGR00398 metG, 1 hit

Sequencei

Sequence statusi: Complete.

P47267-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRCYITTPI YYASGKPHIG HAFTTILADV IKRFKIQNGY EAFLLVGSDE
60 70 80 90 100
HGNKIESKAK SLNLDPKTFV DINAQAFKLM WKTLNISFDH FIRTTDEIHK
110 120 130 140 150
QQVQKTFQDL YDKKLIYQSE WKGAYCVECE QNYFTFNKQT MLCEIGHNLS
160 170 180 190 200
LVQEPCWFIS FSSTKNWIET TIGKNQLNII PKSRASELKN NFINNGLNDL
210 220 230 240 250
ALTRKNVTWG IKVPFDPNQT IYVWFDALFS YITNLGFRNG DPNFIKWWNN
260 270 280 290 300
DNKEREVIHL ISREITRFHC IYWPIFLHLL DIKLPTQFLS HGWIVDGEGR
310 320 330 340 350
KMSKSLNNVI SPEQLIDQFG VDGTRYCLLK EMRLDKDNRC SVSILKEIYN
360 370 380 390 400
ADLANSFGNH VSRTFGMIKK YLNGKLEYQI ITDNALQKIM ILIDESIVQF
410 420 430 440 450
DHYFNSYEFY RAINLLLKIV FELSKLIDDF KPWELFKNQE FSLLKQLLFT
460 470 480 490 500
CVRCVQVCYV LLTPILVNTA SKVFHLFNFA DDACRKDQLR DATLLKKIII
510
SNSMEVLFKR VD
Length:512
Mass (Da):60,007
Last modified:February 1, 1996 - v1
Checksum:iCA05EC9B4356F432
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti506 – 508VLF → FYL (PubMed:1945886).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L43967 Genomic DNA Translation: AAC71237.1
U02229 Genomic DNA Translation: AAA03382.2
X61513 Genomic DNA Translation: CAA43727.1
PIRiC64202
RefSeqiWP_009885919.1, NZ_AAGX01000010.1

Genome annotation databases

EnsemblBacteriaiAAC71237; AAC71237; MG_021
KEGGimge:MG_021

Similar proteinsi

Entry informationi

Entry nameiSYM_MYCGE
AccessioniPrimary (citable) accession number: P47267
Secondary accession number(s): Q49339, Q49473
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 123 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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