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P47267

- SYM_MYCGE

UniProt

P47267 - SYM_MYCGE

Protein

Methionine--tRNA ligase

Gene

metG

Organism
Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 99 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.By similarity

    Catalytic activityi

    ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met).

    Cofactori

    Binds 1 zinc ion per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi126 – 1261ZincBy similarity
    Metal bindingi129 – 1291ZincBy similarity
    Metal bindingi143 – 1431ZincBy similarity
    Metal bindingi147 – 1471ZincBy similarity
    Binding sitei304 – 3041ATPBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW
    3. methionine-tRNA ligase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. methionyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciMGEN243273:GH2R-21-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Methionine--tRNA ligase (EC:6.1.1.10)
    Alternative name(s):
    Methionyl-tRNA synthetase
    Short name:
    MetRS
    Gene namesi
    Name:metG
    Synonyms:metS
    Ordered Locus Names:MG021
    OrganismiMycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)
    Taxonomic identifieri243273 [NCBI]
    Taxonomic lineageiBacteriaTenericutesMollicutesMycoplasmataceaeMycoplasma
    ProteomesiUP000000807: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 512512Methionine--tRNA ligasePRO_0000139228Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.By similarity

    Protein-protein interaction databases

    STRINGi243273.MG_021.

    Structurei

    3D structure databases

    ProteinModelPortaliP47267.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi11 – 2111"HIGH" regionAdd
    BLAST
    Motifi301 – 3055"KMSKS" region

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0143.
    KOiK01874.
    OMAiSHAHWTM.
    OrthoDBiEOG6CVV9B.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPiMF_01228. Met_tRNA_synth_type2.
    InterProiIPR014758. Met-tRNA_synth.
    IPR023457. Met-tRNA_synth_2.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view]
    PfamiPF09334. tRNA-synt_1g. 1 hit.
    [Graphical view]
    PRINTSiPR01041. TRNASYNTHMET.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    TIGRFAMsiTIGR00398. metG. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P47267-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKRCYITTPI YYASGKPHIG HAFTTILADV IKRFKIQNGY EAFLLVGSDE    50
    HGNKIESKAK SLNLDPKTFV DINAQAFKLM WKTLNISFDH FIRTTDEIHK 100
    QQVQKTFQDL YDKKLIYQSE WKGAYCVECE QNYFTFNKQT MLCEIGHNLS 150
    LVQEPCWFIS FSSTKNWIET TIGKNQLNII PKSRASELKN NFINNGLNDL 200
    ALTRKNVTWG IKVPFDPNQT IYVWFDALFS YITNLGFRNG DPNFIKWWNN 250
    DNKEREVIHL ISREITRFHC IYWPIFLHLL DIKLPTQFLS HGWIVDGEGR 300
    KMSKSLNNVI SPEQLIDQFG VDGTRYCLLK EMRLDKDNRC SVSILKEIYN 350
    ADLANSFGNH VSRTFGMIKK YLNGKLEYQI ITDNALQKIM ILIDESIVQF 400
    DHYFNSYEFY RAINLLLKIV FELSKLIDDF KPWELFKNQE FSLLKQLLFT 450
    CVRCVQVCYV LLTPILVNTA SKVFHLFNFA DDACRKDQLR DATLLKKIII 500
    SNSMEVLFKR VD 512
    Length:512
    Mass (Da):60,007
    Last modified:February 1, 1996 - v1
    Checksum:iCA05EC9B4356F432
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti506 – 5083VLF → FYL(PubMed:1945886)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71237.1.
    U02229 Genomic DNA. Translation: AAA03382.2.
    X61513 Genomic DNA. Translation: CAA43727.1.
    PIRiC64202.
    RefSeqiNP_072681.1. NC_000908.2.
    WP_009885919.1. NZ_AAGX01000010.1.

    Genome annotation databases

    EnsemblBacteriaiAAC71237; AAC71237; MG_021.
    GeneIDi875451.
    KEGGimge:MG_021.
    PATRICi20009418. VBIMycGen98045_0019.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L43967 Genomic DNA. Translation: AAC71237.1 .
    U02229 Genomic DNA. Translation: AAA03382.2 .
    X61513 Genomic DNA. Translation: CAA43727.1 .
    PIRi C64202.
    RefSeqi NP_072681.1. NC_000908.2.
    WP_009885919.1. NZ_AAGX01000010.1.

    3D structure databases

    ProteinModelPortali P47267.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 243273.MG_021.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC71237 ; AAC71237 ; MG_021 .
    GeneIDi 875451.
    KEGGi mge:MG_021.
    PATRICi 20009418. VBIMycGen98045_0019.

    Phylogenomic databases

    eggNOGi COG0143.
    KOi K01874.
    OMAi SHAHWTM.
    OrthoDBi EOG6CVV9B.

    Enzyme and pathway databases

    BioCyci MGEN243273:GH2R-21-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 1 hit.
    HAMAPi MF_01228. Met_tRNA_synth_type2.
    InterProi IPR014758. Met-tRNA_synth.
    IPR023457. Met-tRNA_synth_2.
    IPR015413. Methionyl/Leucyl_tRNA_Synth.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    [Graphical view ]
    Pfami PF09334. tRNA-synt_1g. 1 hit.
    [Graphical view ]
    PRINTSi PR01041. TRNASYNTHMET.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    TIGRFAMsi TIGR00398. metG. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    2. "A survey of the Mycoplasma genitalium genome by using random sequencing."
      Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III
      J. Bacteriol. 175:7918-7930(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53.
      Strain: ATCC 33530 / G-37 / NCTC 10195.
    3. "A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium."
      Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III
      Nucleic Acids Res. 19:6027-6031(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-508.
      Strain: ATCC 33530 / G-37 / NCTC 10195.

    Entry informationi

    Entry nameiSYM_MYCGE
    AccessioniPrimary (citable) accession number: P47267
    Secondary accession number(s): Q49339, Q49473
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 99 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. Mycoplasma genitalium
      Mycoplasma genitalium (strain G-37): entries and gene names
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3