Reviewed, UniProtKB/Swiss-Prot P47267 (SYM_MYCGE)

Names and origin

Protein names

Recommended name:
    Methionyl-tRNA synthetase
    EC=6.1.1.10
Alternative name(s):
    Methionine--tRNA ligase
      Short name=MetRS

Gene names

Name:	metG
Synonyms:	metS
Ordered Locus Names:	MG021

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	512 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation By similarity.
Catalytic activity	ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met). HAMAP MF_01228
Cofactor	Binds 1 zinc ion per subunit By similarity.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm HAMAP MF_01228.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family. MetG type 2A subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP methionine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 512	512	Methionyl-tRNA synthetase HAMAP MF_01228		PRO_0000139228
Regions
	Motif	11 – 21	11	"HIGH" region HAMAP MF_01228
	Motif	301 – 305	5	"KMSKS" region HAMAP MF_01228
Sites
	Metal binding	126	1	Zinc By similarity
	Metal binding	129	1	Zinc By similarity
	Metal binding	143	1	Zinc By similarity
	Metal binding	147	1	Zinc By similarity
	Binding site	304	1	ATP By similarity
Experimental info
	Sequence conflict	506 – 508	3	VLF → FYL Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P47267-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: CA05EC9B4356F432	FASTA	512	60,007
	10 20 30 40 50 60 MKRCYITTPI YYASGKPHIG HAFTTILADV IKRFKIQNGY EAFLLVGSDE HGNKIESKAK 70 80 90 100 110 120 SLNLDPKTFV DINAQAFKLM WKTLNISFDH FIRTTDEIHK QQVQKTFQDL YDKKLIYQSE 130 140 150 160 170 180 WKGAYCVECE QNYFTFNKQT MLCEIGHNLS LVQEPCWFIS FSSTKNWIET TIGKNQLNII 190 200 210 220 230 240 PKSRASELKN NFINNGLNDL ALTRKNVTWG IKVPFDPNQT IYVWFDALFS YITNLGFRNG 250 260 270 280 290 300 DPNFIKWWNN DNKEREVIHL ISREITRFHC IYWPIFLHLL DIKLPTQFLS HGWIVDGEGR 310 320 330 340 350 360 KMSKSLNNVI SPEQLIDQFG VDGTRYCLLK EMRLDKDNRC SVSILKEIYN ADLANSFGNH 370 380 390 400 410 420 VSRTFGMIKK YLNGKLEYQI ITDNALQKIM ILIDESIVQF DHYFNSYEFY RAINLLLKIV 430 440 450 460 470 480 FELSKLIDDF KPWELFKNQE FSLLKQLLFT CVRCVQVCYV LLTPILVNTA SKVFHLFNFA 490 500 510 DDACRKDQLR DATLLKKIII SNSMEVLFKR VD

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-53. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium." Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III Nucleic Acids Res. 19:6027-6031(1991) [PubMed: 1945886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 440-508. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71237.1. U02229 Genomic DNA. Translation: AAA03382.2. X61513 Genomic DNA. Translation: CAA43727.1.
PIR	C64202.
RefSeq	NP_072681.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875451.
KEGG	mge:MG_021.
TIGR	MG021.
Phylogenomic databases
HOGENOM	HBG721667.
OMA	WDEFEIS.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_021-MON.
BRENDA	6.1.1.10. 110.
Family and domain databases
HAMAP	MF_01228. [Tree]
InterPro	IPR015413. aa-tRNA-synt_I. IPR002304. Met-tRNA-synth_Ia. IPR014729. Rossmann-like_a/b/a_fold. IPR014758. tRNA-synt_met_N. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
Pfam	PF09334. tRNA-synt_1g. 1 hit. [Graphical view]
PRINTS	PR01041. TRNASYNTHMET.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYM_MYCGE

Accession

Primary (citable) accession number: P47267
Secondary accession number(s): Q49339, Q49473

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 15, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)