Reviewed, UniProtKB/Swiss-Prot P47266 (PIP_MYCGE)

Names and origin

Protein names

Recommended name:
    Putative proline iminopeptidase
      Short name=PIP
    EC=3.4.11.5
Alternative name(s):
    Prolyl aminopeptidase
      Short name=PAP

Gene names

Name:	pip
Ordered Locus Names:	MG020

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Specifically catalyzes the removal of N-terminal proline residues from peptides By similarity.
Catalytic activity	Release of N-terminal proline from a peptide.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Cellular component	Cytoplasm
Molecular function	Aminopeptidase Hydrolase Protease
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Putative proline iminopeptidase		PRO_0000080839
Sites
	Active site	105	1	Nucleophile By similarity
	Active site	261	1	By similarity
	Active site	289	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47266-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 65087048D205EEF3	FASTA	308	35,289
	10 20 30 40 50 60 MNTKLNVKGY LNVGDNHQLY YWTQGNPNGK PVLYIHGGPG SGTDEGCLKY FDLETTWIIL 70 80 90 100 110 120 LDQRGCGKSK TNDIFYENNT DKLVSDFEIL RQKLNIKNWT LFGGSWGSAL ALVYAIKHPQ 130 140 150 160 170 180 VVDKIFLRAL FLAREKDWSE ALMGLGKMFY PYEHQRFMDS IPKAYQNSYE QIVNYCYDQF 190 200 210 220 230 240 QNGDESTKEK LAKAWVDWES TLLSPINKIH STATDFKLVE KLALLECHYA VNKSFLDENF 250 260 270 280 290 300 ILDNISVLKN KSIYLAHGRF DLICPLYQPL ALKQAFPELQ LYVTNNAGHS GSDANNLATI KHLLKTYL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 242-308. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71236.1. U02229 Genomic DNA. Translation: AAA03381.1.
PIR	B64202.
RefSeq	NP_072680.1.
3D structure databases
HSSP	HSSP built from PDB template 1AZW based on UniProtKB P52279.
ModBase	Search...
Protein family/group databases
MEROPS	S33.001.
Genome annotation databases
GeneID	875529.
GenomeReviews	Gene locus MG020 in contig L43967_GR.
KEGG	mge:MG_020.
TIGR	MG020.
Phylogenomic databases
HOGENOM	P47266.
OMA	DWCAWED.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_020-MON.
BRENDA	3.4.11.5. 110.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR005944. Pept_S33. IPR002410. Peptidase_S33. [Graphical view]
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PIRSF	PIRSF006431. Pept_S33. 1 hit.
PRINTS	PR00793. PROAMNOPTASE.
ProDom	PD001189. Peptidase_S10. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01249. pro_imino_pep_1. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PIP_MYCGE

Accession

Primary (citable) accession number: P47266

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)