Reviewed, UniProtKB/Swiss-Prot P47265 (DNAJ_MYCGE)

Names and origin

Protein names

Recommended name:
    Chaperone protein dnaJ

Gene names

Name:	dnaJ
Ordered Locus Names:	MG019

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	389 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins and by disaggregating proteins, also in an autonomous, dnaK-independent fashion. Unfolded proteins bind initially to dnaJ; upon interaction with the dnaJ-bound protein, dnaK hydrolyzes its bound ATP, resulting in the formation of a stable complex. GrpE releases ADP from dnaK; ATP binding to dnaK triggers the release of the substrate protein, thus completing the reaction cycle. Several rounds of ATP-dependent interactions between dnaJ, dnaK and grpE are required for fully efficient folding. Also involved, together with dnaK and grpE, in the DNA replication of plasmids through activation of initiation proteins By similarity.
Cofactor	Binds 2 zinc ions per monomer By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm Probable.
Domain	The J domain is necessary and sufficient to stimulate dnaK ATPase activity. Zinc center 1 plays an important role in the autonomous, dnaK-independent chaperone activity of dnaJ. Zinc center 2 is essential for interaction with dnaK and for dnaJ activity By similarity.
Sequence similarities	Belongs to the dnaJ family. Contains 1 CR-type zinc finger. Contains 1 J domain.

Ontologies

Keywords
Biological process	DNA replication Stress response
Cellular component	Cytoplasm
Domain	Repeat Zinc-finger
Ligand	Metal-binding Zinc
Molecular function	Chaperone
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW protein folding Inferred from electronic annotation. Source: InterPro response to heat Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro heat shock protein binding Inferred from electronic annotation. Source: InterPro unfolded protein binding Inferred from electronic annotation. Source: HAMAP zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 389	389	Chaperone protein dnaJ HAMAP MF_01152		PRO_0000070824
Regions
	Domain	5 – 79	75	J
	Repeat	164 – 171	8	CXXCXGXG motif HAMAP MF_01152
	Repeat	182 – 189	8	CXXCXGXG motif HAMAP MF_01152
	Repeat	208 – 215	8	CXXCXGXG motif HAMAP MF_01152
	Repeat	222 – 229	8	CXXCXGXG motif HAMAP MF_01152
	Zinc finger	151 – 234	84	CR-type HAMAP MF_01152
	Compositional bias	84 – 134	51	Gly-rich HAMAP MF_01152
Sites
	Metal binding	164	1	Zinc 1 By similarity
	Metal binding	167	1	Zinc 1 By similarity
	Metal binding	182	1	Zinc 2 By similarity
	Metal binding	185	1	Zinc 2 By similarity
	Metal binding	208	1	Zinc 2 By similarity
	Metal binding	211	1	Zinc 2 By similarity
	Metal binding	222	1	Zinc 1 By similarity
	Metal binding	225	1	Zinc 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47265-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 10614543F4B6ACDE	FASTA	389	43,746
	10 20 30 40 50 60 MAAGKRDYYE VLGISKNASS QDIKRAFRKL AMQYHPDRHK AENETTQKQN EEKFKEVNEA 70 80 90 100 110 120 YEVLSDEEKR KLYDQFGHEG LNASGFHEAG FNPFDIFNSV FGEGFSFGMD GDSPFDFIFN 130 140 150 160 170 180 RSKKRQQQIV VPYNLDIALV IEINFFEMTN GCNKTIKYER KVSCHSCNGF GAEGGESGLD 190 200 210 220 230 240 LCKDCNGNGF VIKNQRSIFG TIQSQVLCST CNGQGKQIKV KCKTCRSNKY TVTNQIKEIN 250 260 270 280 290 300 IPAGMYSGEA LVDESGGNEF KGHYGKLIIQ VNVLASKIFK RSDNNVIANV LVDPMVAIVG 310 320 330 340 350 360 GVIELPTLEG IKEFNIRPGT KSGEQIVIPN GGIKFSKSFK RKAGDLIIII SYARPCEYTN 370 380 LELKKLREFI KPNQEVKQYL NTLKNEYKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-15. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71235.1. U01723 Genomic DNA. Translation: AAC43198.1.
PIR	A64202.
RefSeq	NP_072679.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875219.
GenomeReviews	Gene locus MG019 in contig L43967_GR.
KEGG	mge:MG_019.
TIGR	MG019.
Phylogenomic databases
HOGENOM	P47265.
OMA	FEHGGMG
Enzyme and pathway databases
BioCyc	MGEN243273:MG_019-MON.
Family and domain databases
HAMAP	MF_01152. [Tree]
InterPro	IPR012724. DnaJ_bact. IPR002939. DnaJ_C. IPR001623. DnaJ_N. IPR018253. Heat_shock_DnaJ_CS. IPR008971. HSP40/DnaJ_pept-bd. IPR015609. Hsp40/DnaJ_Rel. IPR003095. Hsp_DnaJ. IPR001305. HSP_DnaJ_cys-rich. [Graphical view]
Gene3D	G3DSA:1.10.287.110. DnaJ_N. 1 hit. G3DSA:2.10.230.10. HSP_DnaJ_cys-rich. 1 hit.
PANTHER	PTHR11821. Hsp40/DnaJ_Rel. 1 hit.
Pfam	PF00226. DnaJ. 1 hit. PF01556. DnaJ_C. 1 hit. PF00684. DnaJ_CXXCXGXG. 1 hit. [Graphical view]
PRINTS	PR00625. DNAJPROTEIN.
SMART	SM00271. DnaJ. 1 hit. [Graphical view]
TIGRFAMs	TIGR02349. DnaJ_bact. 1 hit.
PROSITE	PS00636. DNAJ_1. 1 hit. PS50076. DNAJ_2. 1 hit. PS51188. ZF_CR. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAJ_MYCGE

Accession

Primary (citable) accession number: P47265

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)