Reviewed, UniProtKB/Swiss-Prot P47254 (MNME_MYCGE)

Names and origin

Protein names

Recommended name:
    tRNA modification GTPase mnmE
    EC=3.6.-.-

Gene names

Name:	mnmE
Synonyms:	thdF, trmE
Ordered Locus Names:	MG008

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm5s2U34 By similarity.
Cofactor	Binds 1 potassium ion per subunit By similarity.
Subunit structure	Homodimer By similarity. Heterotetramer of two mnmE and two mnmG subunits By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the era/mnmE GTP-binding protein family. MnmE subfamily.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: HAMAP GTPase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	tRNA modification GTPase mnmE HAMAP MF_00379		PRO_0000188891
Regions
	Nucleotide binding	227 – 232	6	GTP By similarity
	Nucleotide binding	246 – 252	7	GTP By similarity
	Nucleotide binding	271 – 274	4	GTP By similarity
Sites
	Metal binding	227	1	Potassium By similarity
	Metal binding	231	1	Magnesium By similarity
	Metal binding	246	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	248	1	Potassium; via carbonyl oxygen By similarity
	Metal binding	251	1	Potassium By similarity
	Metal binding	252	1	Magnesium By similarity
	Binding site	23	1	Formyltetrahydrofolate By similarity
	Binding site	82	1	Formyltetrahydrofolate By similarity
	Binding site	121	1	Formyltetrahydrofolate By similarity
	Binding site	442	1	Formyltetrahydrofolate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47254-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: C6D50D3274B0EB32	FASTA	442	50,806
	10 20 30 40 50 60 MKSEINIFAL ATAPFNSALH IIRFSGPDVY EILNKITNKK ITRKGMQIQR TWIVDENNKR 70 80 90 100 110 120 IDDVLLFKFV SPNSYTGEDL IEISCHGNML IVNEICALLL KKGGVYAKPG EFTQRSFLNG 130 140 150 160 170 180 KMSLQQASAV NKLILSPNLL VKDIVLNNLA GEMDQQLEQI AQQVNQLVMQ MEVNIDYPEY 190 200 210 220 230 240 LDEQVELSTL NNKVKLIIEK LKRIIENSKQ LKKLHDPFKI AIIGETNVGK SSLLNALLNQ 250 260 270 280 290 300 DKAIVSNIKG STRDVVEGDF NLNGYLIKIL DTAGIRKHKS GLEKAGIKKS FESIKQANLV 310 320 330 340 350 360 IYLLDATHPK KDLELISFFK KNKKDFFVFY NKKDLITNKF ENSISAKQKD IKELVDLLTK 370 380 390 400 410 420 YINEFYKKID QKIYLIENWQ QILIEKIKEQ LEQFLKQQKK YLFFDVLVTH LREAQQDILK 430 440 LLGKDVGFDL VNEIFNNFCL GK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L43967 Genomic DNA. Translation: AAC71224.1. U02216 Genomic DNA. Translation: AAD12512.1. Different initiation.
PIR	H64200.
RefSeq	NP_072668.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	875731.
GenomeReviews	Gene locus MG008 in contig L43967_GR.
KEGG	mge:MG_008.
TIGR	MG008.
Phylogenomic databases
HOGENOM	P47254.
OMA	VVEIACH
Enzyme and pathway databases
BioCyc	MGEN243273:MG_008-MON.
Family and domain databases
HAMAP	MF_00379. [Tree]
InterPro	IPR018948. GTP_bd_TrmE_N. IPR002917. MMR_HSR1_GTP_bd. IPR005225. Small_GTP_bd. IPR004520. ThdF. [Graphical view]
Gene3D	G3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
Pfam	PF01926. MMR_HSR1. 1 hit. PF10396. TrmE_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00450. mnmE_trmE_thdF. 1 hit. TIGR00231. small_GTP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNME_MYCGE

Accession

Primary (citable) accession number: P47254
Secondary accession number(s): Q49330

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)