tRNA modification GTPase MnmE - Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195)

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P47254 (MNME_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 76. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
tRNA modification GTPase MnmE
EC=3.6.-.-

Gene names

Name:	mnmE
Synonyms:	thdF, trmE
Ordered Locus Names:	MG008

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm₅s₂U34 By similarity. HAMAP MF_00379
Cofactor	Binds 1 potassium ion per subunit By similarity. HAMAP MF_00379
Subunit structure	Homodimer By similarity. Heterotetramer of two MnmE and two MnmG subunits By similarity. HAMAP MF_00379
Subcellular location	Cytoplasm By similarity HAMAP MF_00379.
Sequence similarities	Belongs to the Era/MnmE GTP-binding protein family. MnmE subfamily. Contains 1 G (guanine nucleotide-binding) domain.
Sequence caution	The sequence AAD12512.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Cytoplasm
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA modification Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 442

442

tRNA modification GTPase MnmE HAMAP MF_00379

PRO_0000188891

Regions

Domain

229 – 332

104

Nucleotide binding

227 – 232

GTP By similarity

Nucleotide binding

246 – 252

GTP By similarity

Nucleotide binding

271 – 274

GTP By similarity

Sites

Metal binding

227

Potassium By similarity

Metal binding

231

Magnesium By similarity

Metal binding

246

Potassium; via carbonyl oxygen By similarity

Metal binding

248

Potassium; via carbonyl oxygen By similarity

Metal binding

251

Potassium By similarity

Metal binding

252

Magnesium By similarity

Binding site

Formyltetrahydrofolate By similarity

Binding site

Formyltetrahydrofolate By similarity

Binding site

121

Formyltetrahydrofolate By similarity

Binding site

442

Formyltetrahydrofolate By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P47254 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: C6D50D3274B0EB32
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FASTA

442

50,806

        10         20         30         40         50         60 
MKSEINIFAL ATAPFNSALH IIRFSGPDVY EILNKITNKK ITRKGMQIQR TWIVDENNKR 

        70         80         90        100        110        120 
IDDVLLFKFV SPNSYTGEDL IEISCHGNML IVNEICALLL KKGGVYAKPG EFTQRSFLNG 

       130        140        150        160        170        180 
KMSLQQASAV NKLILSPNLL VKDIVLNNLA GEMDQQLEQI AQQVNQLVMQ MEVNIDYPEY 

       190        200        210        220        230        240 
LDEQVELSTL NNKVKLIIEK LKRIIENSKQ LKKLHDPFKI AIIGETNVGK SSLLNALLNQ 

       250        260        270        280        290        300 
DKAIVSNIKG STRDVVEGDF NLNGYLIKIL DTAGIRKHKS GLEKAGIKKS FESIKQANLV 

       310        320        330        340        350        360 
IYLLDATHPK KDLELISFFK KNKKDFFVFY NKKDLITNKF ENSISAKQKD IKELVDLLTK 

       370        380        390        400        410        420 
YINEFYKKID QKIYLIENWQ QILIEKIKEQ LEQFLKQQKK YLFFDVLVTH LREAQQDILK 

       430        440 
LLGKDVGFDL VNEIFNNFCL GK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-88. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71224.1. U02216 Genomic DNA. Translation: AAD12512.1. Different initiation.
PIR	H64200.
RefSeq	NP_072668.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47254.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875731.
GenomeReviews	Gene locus MG008 in contig L43967_GR.
KEGG	mge:MG_008.
PATRIC	20009392. VBIMycGen98045_0008.
TIGR	MG008.
Phylogenomic databases
HOGENOM	HBG746795.
OMA	SEINIFA.
PhylomeDB	P47254.
ProtClustDB	PRK05291.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_008-MONOMER.
Family and domain databases
HAMAP	MF_00379. GTPase_MnmE. [Tree]
InterPro	IPR018948. GTP-bd_TrmE_N. IPR006073. GTP_binding_domain. IPR004520. GTPase_TrmE. IPR005225. Small_GTP-bd_dom. [Graphical view]
Gene3D	G3DSA:3.30.1360.120. GTP_bd_TrmE_N. 1 hit.
KO	K03650.
Pfam	PF01926. MMR_HSR1. 1 hit. PF10396. TrmE_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00450. MnmE_trmE_thdF. 1 hit. TIGR00231. Small_GTP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MNME_MYCGE

Accession

Primary (citable) accession number: P47254
Secondary accession number(s): Q49330

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Mycoplasma genitalium

Mycoplasma genitalium (strain G-37): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protocols and materials databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Entry information

Relevant documents