Reviewed, UniProtKB/Swiss-Prot P47250 (GYRA_MYCGE)

Names and origin

Protein names

Recommended name:
    DNA gyrase subunit A
    EC=5.99.1.3

Gene names

Name:	gyrA
Ordered Locus Names:	MG004

Organism

Mycoplasma genitalium [Complete proteome] [HAMAP]

Taxonomic identifier

2097 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	836 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings.
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA.
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Sequence similarities	Belongs to the topoisomerase gyrA/parC subunit family.

Ontologies

Keywords
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 836	836	DNA gyrase subunit A		PRO_0000145240
Sites
	Active site	134	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity
Experimental info
	Sequence conflict	68 – 76	9	GMHHDRPFK → WSCTMIVLL Ref.3
	Sequence conflict	648	1	R → L in AAA57072. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P47250-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 364A78ED5B060BCE	FASTA	836	93,682
	10 20 30 40 50 60 MAKQQDQVDK IRENLDNSTV KSISLANELE RSFMEYAMSV IVARALPDAR DGLKPVHRRV 70 80 90 100 110 120 LYGAYIGGMH HDRPFKKSAR IVGDVMSKFH PHGDMAIYDT MSRMAQDFSL RYLLIDGHGN 130 140 150 160 170 180 FGSIDGDRPA AQRYTEARLS KLAAELLKDI DKDTVDFIAN YDGEEKEPTV LPAAFPNLLA 190 200 210 220 230 240 NGSSGIAVGM STSIPSHNLS ELIAGLIMLI DNPQCTFQEL LTVIKGPDFP TGANIIYTKG 250 260 270 280 290 300 IESYFETGKG NVVIRSKVEI EQLQTRSALV VTEIPYMVNK TTLIEKIVEL VKAEEISGIA 310 320 330 340 350 360 DIRDESSREG IRLVIEVKRD TVPEVLLNQL FKSTRLQVRF PVNMLALVKG APVLLNMKQA 370 380 390 400 410 420 LEVYLDHQID VLVRKTKFVL NKQQERYHIL SGLLIAALNI DEVVAIIKKS ANNQEAINTL 430 440 450 460 470 480 NTKFKLDEIQ AKAVLDMRLR SLSVLEVNKL QTEQKELKDS IEFCKKVLAD QKLQLKIIKE 490 500 510 520 530 540 ELQKINDQFG DERRSEILYD ISEEIDDESL IKVENVVITM STNGYLKRIG VDAYNLQHRG 550 560 570 580 590 600 GVGVKGLTTY VDDSISQLLV CSTHSDLLFF TDKGKVYRIR AHQIPYGFRT NKGIPAVNLI 610 620 630 640 650 660 KIEKDERICS LLSVNNYDDG YFFFCTKNGI VKRTSLNEFI NILSNGKRAI SFDDNDTLYS 670 680 690 700 710 720 VIKTHGNDEI FIGSTNGFVV RFHENQLRVL SRTARGVFGI SLNKGEFVNG LSTSSNGSLL 730 740 750 760 770 780 LSVGQNGIGK LTSIDKYRLT KRNAKGVKTL RVTDRTGPVV TTTTVFGNED LLMISSAGKI 790 800 810 820 830 VRTSLQELSE QGKNTSGVKL IRLKDNERLE RVTIFKEELE DKEMQLEDVG SKQITQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium." Bailey C.C., Bott K.F. J. Bacteriol. 176:5814-5819(1994) [PubMed: 8083173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-77 AND 205-302. Strain: ATCC 33530 / G-37 / NCTC 10195.
[4]	"A random sequencing approach for placing markers on the physical map of Mycoplasma genitalium." Peterson S.N., Schramm N., Hu P.-C., Bott K.F., Hutchison C.A. III Nucleic Acids Res. 19:6027-6031(1991) [PubMed: 1945886] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 509-639. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09251 Genomic DNA. Translation: AAA57072.1. L43967 Genomic DNA. Translation: AAC71220.1. U02211 Genomic DNA. Translation: AAD12504.1. U01696 Genomic DNA. Translation: AAB01009.1. X61533 Genomic DNA. Translation: CAA43745.1.
PIR	D64200.
RefSeq	NP_072664.1.
3D structure databases
SMR	P47250. Positions 42-497, 514-816.
ModBase	Search...
Genome annotation databases
GeneID	875558.
GenomeReviews	Gene locus MG004 in contig L43967_GR.
KEGG	mge:MG_004.
TIGR	MG004.
Phylogenomic databases
HOGENOM	HBG635183.
OMA	NTHDILL.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_004-MONOMER.
BRENDA	5.99.1.3. 110.
Family and domain databases
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
TIGRFAMs	TIGR01063. gyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_MYCGE

Accession

Primary (citable) accession number: P47250
Secondary accession number(s): Q49325

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 19, 2010
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)