P47249 (GYRB_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit B
EC=5.99.1.3

Gene names

Name:	gyrB
Ordered Locus Names:	MG003

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	650 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01898
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01898
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01898.
Sequence similarities	Belongs to the type II topoisomerase family. Contains 1 Toprim domain.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 650	650	DNA gyrase subunit B HAMAP MF_01898		PRO_0000145319
Regions
	Domain	435 – 546	112	Toprim

Sequences

	Sequence		Length	Mass (Da)
	P47249 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 750BA6035A5CB55E	FASTA	650	73,605
	10 20 30 40 50 60 MEENNKANIY DSSSIKVLEG LEAVRKRPGM YIGSTGEEGL HHMIWEIVDN SIDEAMGGFA 70 80 90 100 110 120 SFVKLTLEDN FVTRVEDDGR GIPVDIHPKT NRSTVETVFT VLHAGGKFDN DSYKVSGGLH 130 140 150 160 170 180 GVGASVVNAL SSSFKVWVFR QNKKYFLSFS DGGKVIGDLV QEGNSEKEHG TIVEFVPDFS 190 200 210 220 230 240 VMEKSDYKQT VIVSRLQQLA FLNKGIRIDF VDNRKQNPQS FSWKYDGGLV EYIHHLNNEK 250 260 270 280 290 300 EPLFNEVIAD EKTETVKAVN RDENYTVKVE VAFQYNKTYN QSIFSFCNNI NTTEGGTHVE 310 320 330 340 350 360 GFRNALVKII NRFAVENKFL KDSDEKINRD DVCEGLTAII SIKHPNPQYE GQTKKKLGNT 370 380 390 400 410 420 EVRPLVNSVV SEIFERFMLE NPQEANAIIR KTLLAQEARR RSQEARELTR RKSPFDSGSL 430 440 450 460 470 480 PGKLADCTTR DPSISELYIV EGDSAGGTAK TGRDRYFQAI LPLRGKILNV EKSNFEQIFN 490 500 510 520 530 540 NAEISALVMA IGCGIKPDFE LEKLRYSKIV IMTDADVDGA HIRTLLLTFF FRFMYPLVEQ 550 560 570 580 590 600 GNIFIAQPPL YKVSYSHKDL YMHTDVQLEQ WKSQNPNVKF GLQRYKGLGE MDALQLWETT 610 620 630 640 650 MDPKVRTLLK VTVEDASIAD KAFSLLMGDE VPPRREFIEK NARSVKNIDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium." Bailey C.C., Bott K.F. J. Bacteriol. 176:5814-5819(1994) [PubMed: 8083173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[3]	"A survey of the Mycoplasma genitalium genome by using random sequencing." Peterson S.N., Hu P.-C., Bott K.F., Hutchison C.A. III J. Bacteriol. 175:7918-7930(1993) [PubMed: 8253680] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 199-304; 404-507 AND 605-650. Strain: ATCC 33530 / G-37 / NCTC 10195.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U09251 Genomic DNA. Translation: AAA57071.1. L43967 Genomic DNA. Translation: AAC71219.1. U02187 Genomic DNA. Translation: AAD12471.1. U02199 Genomic DNA. Translation: AAD12487.1. U02211 Genomic DNA. Translation: AAD12503.1.
PIR	C64200.
RefSeq	NP_072663.1. NC_000908.2.
3D structure databases
ProteinModelPortal	P47249.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875545.
GenomeReviews	Gene locus MG003 in contig L43967_GR.
KEGG	mge:MG_003.
PATRIC	20009382. VBIMycGen98045_0003.
TIGR	MG003.
Phylogenomic databases
HOGENOM	HBG304889.
OMA	MDRILAN.
PhylomeDB	P47249.
ProtClustDB	PRK05644.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_003-MONOMER.
Family and domain databases
HAMAP	MF_01898. GyrB. [Tree]
InterPro	IPR003594. ATPase-like_ATP-bd. IPR011557. GyrB. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR001241. Topo_IIA. IPR013759. Topo_IIA_B/N_ab. IPR002288. Topo_IIA_B_C. IPR013506. Topo_IIA_bsu_dom2. IPR013760. Topo_IIA_cen. IPR018522. TopoIIA_CS. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:3.30.565.10. ATP_bd_ATPase. 1 hit. G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit. G3DSA:3.40.50.670. Topo_IIA_B/N_ab. 1 hit.
KO	K02470.
Pfam	PF00204. DNA_gyraseB. 1 hit. PF00986. DNA_gyraseB_C. 1 hit. PF02518. HATPase_c. 1 hit. PF01751. Toprim. 1 hit. [Graphical view]
PRINTS	PR00418. TPI2FAMILY.
SMART	SM00387. HATPase_c. 1 hit. SM00433. TOP2c. 1 hit. [Graphical view]
SUPFAM	SSF55874. ATP_bd_ATPase. 1 hit. SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit. SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01059. GyrB. 1 hit.
PROSITE	PS00177. TOPOISOMERASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GYRB_MYCGE

Accession

Primary (citable) accession number: P47249
Secondary accession number(s): Q49307

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program