P47247 (DPO3B_MYCGE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA polymerase III subunit beta
EC=2.7.7.7

Gene names

Name:	dnaN
Ordered Locus Names:	MG001

Organism

Mycoplasma genitalium (strain ATCC 33530 / G-37 / NCTC 10195) [Complete proteome] [HAMAP]

Taxonomic identifier

243273 [NCBI]

Taxonomic lineage

Bacteria › Tenericutes › Mollicutes › Mycoplasmataceae › Mycoplasma

Protein attributes

Sequence length	364 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped onto DNA, it slides freely (bidirectional and ATP-independent) along duplex DNA By similarity.
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
Subunit structure	DNA polymerase III contains a core (composed of alpha, epsilon and theta chains) that associates with a tau subunit. This core dimerizes to form the POLIII' complex. PolIII' associates with the gamma complex (composed of gamma, delta, delta', psi and chi chains) and with the beta chain to form the complete DNA polymerase III complex By similarity.
Subcellular location	Cytoplasm By similarity.

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	DNA polymerase III complex Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 364	364	DNA polymerase III subunit beta		PRO_0000105445

Sequences

	Sequence		Length	Mass (Da)
	P47247 [UniParc]. Last modified May 30, 2000. Version 2. Checksum: 4D8C23EF9BC48624	FASTA	364	42,399
	10 20 30 40 50 60 MNNVIISNNK IKPHHSYFLI EAKEKEINFY ANNEYFSVKC NLNKNIDILE QGSLIVKGKI 70 80 90 100 110 120 FNDLINGIKE EIITIQEKDQ TLLVKTKKTS INLNTINVNE FPRIRFNEKN DLSEFNQFKI 130 140 150 160 170 180 NYSLLVKGIK KIFHSVSNNR EISSKFNGVN FNGSNGKEIF LEASDTYKLS VFEIKQETEP 190 200 210 220 230 240 FDFILESNLL SFINSFNPEE DKSIVFYYRK DNKDSFSTEM LISMDNFMIS YTSVNEKFPE 250 260 270 280 290 300 VNYFFEFEPE TKIVVQKNEL KDALQRIQTL AQNERTFLCD MQINSSELKI RAIVNNIGNS 310 320 330 340 350 360 LEEISCLKFE GYKLNISFNP SSLLDHIESF ESNEINFDFQ GNSKYFLITS KSEPELKQIL VPSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The minimal gene complement of Mycoplasma genitalium." Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Science 270:397-403(1995) [PubMed: 7569993] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 33530 / G-37 / NCTC 10195.
[2]	Fraser C.M., Gocayne J.D., White O., Adams M.D., Clayton R.A., Fleischmann R.D., Bult C.J., Kerlavage A.R., Sutton G.G., Kelley J.M., Fritchman J.L., Weidman J.F., Small K.V., Sandusky M., Fuhrmann J.L., Nguyen D.T., Utterback T.R., Saudek D.M. , Phillips C.A., Merrick J.M., Tomb J.-F., Dougherty B.A., Bott K.F., Hu P.-C., Lucier T.S., Peterson S.N., Smith H.O., Hutchison C.A. III, Venter J.C. Submitted (OCT-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"An unusual gene containing a dnaJ N-terminal box flanks the putative origin of replication of Mycoplasma genitalium." Bailey C.C., Bott K.F. J. Bacteriol. 176:5814-5819(1994) [PubMed: 8083173] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 267-364. Strain: ATCC 33530 / G-37 / NCTC 10195.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43967 Genomic DNA. Translation: AAC71217.1. U09251 Genomic DNA. Translation: AAA57069.1.
RefSeq	NP_072661.2. NC_000908.2.
3D structure databases
ProteinModelPortal	P47247.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	875454.
GenomeReviews	Gene locus MG001 in contig L43967_GR.
KEGG	mge:MG_001.
PATRIC	20009378. VBIMycGen98045_0001.
TIGR	MG001.
Phylogenomic databases
HOGENOM	HBG129976.
OMA	ISSINEW.
PhylomeDB	P47247.
ProtClustDB	CLSK437066.
Enzyme and pathway databases
BioCyc	MGEN243273:MG_001-MONOMER.
Family and domain databases
InterPro	IPR001001. DNA_polIII_beta. IPR022635. DNA_polIII_beta_C. IPR024693. DNA_polIII_beta_dom. IPR022634. DNA_polIII_beta_N. [Graphical view]
Gene3D	G3DSA:3.10.150.10. DNA_polIII_beta. 3 hits.
KO	K02338.
Pfam	PF00712. DNA_pol3_beta. 1 hit. PF02768. DNA_pol3_beta_3. 1 hit. [Graphical view]
SMART	SM00480. POL3Bc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00663. Dnan. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DPO3B_MYCGE

Accession

Primary (citable) accession number: P47247

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	May 30, 2000
Last modified:	January 25, 2012
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program