Reviewed, UniProtKB/Swiss-Prot P47243 (DBFB_PSEPA)

Names and origin

Protein names

Recommended name:
    2,2',3-trihydroxybiphenyl dioxygenase
    EC=1.13.11.-

Gene names

Name:

dbfB

Organism

Pseudomonas paucimobilis (Sphingomonas paucimobilis)

Taxonomic identifier

13689 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Sphingomonadales › Sphingomonadaceae › Sphingomonas

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Responsible for meta-cleavage of the first aromatic ring of 2,2',3-trihydroxybiphenyl and 2,3-dihydroxybiphenyl. 2,2',3-trihydroxydiphenyl ether, catechol, 3-methylcatechol, and 4-methylcatechol are oxidized less efficiently and 3,4-dihydroxybiphenyl is oxidized considerably less efficiently.
Cofactor	Fe2+ ion.
Pathway	Xenobiotic degradation; dibenzo-p-dioxin degradation; 2-hydroxymuconic acid and catechol from dibenzo-p-dioxin: step 2/3. Xenobiotic degradation; dibenzofuran degradation; 2-hydroxy-2,4-pentadienoic acid and salicylic acid from dibenzofuran: step 2/3.
Subunit structure	Monomer.
Sequence similarities	Belongs to the extradiol ring-cleavage dioxygenase family.

Ontologies

Keywords
Biological process	Aromatic hydrocarbons catabolism
Ligand	Iron Metal-binding
Molecular function	Dioxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic compound catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ferrous iron binding Inferred from electronic annotation. Source: InterPro oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed Ref.1
	Chain	2 – 294	293	2,2',3-trihydroxybiphenyl dioxygenase		PRO_0000085041
Sites
	Metal binding	147	1	Iron By similarity
	Metal binding	209	1	Iron By similarity
	Metal binding	260	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47243-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 9315F5B922E1BF8D	FASTA	294	32,278
	10 20 30 40 50 60 MSVKQLGYLI FECRADVLEQ MVVVYQDIIG AVVERDEGGR ALVRLDGRPF RIRLDPGPAN 70 80 90 100 110 120 RLAAIGWNVD PSDLAAIAEQ VEKACYSVVT ADAELAADRA AAQVRQFADN DGFTHELYVE 130 140 150 160 170 180 SSFPTDPVLE SLFVCGEEAN GIFGLGHLVV IVADRAKTQS FFTDVLGFGL SDRVTWPEAD 190 200 210 220 230 240 IFFLHCNQRH HTVALSAPAL GLKPGMVHHL MLEAKSKEQV DRAFAAVKRL GYDVLMTIGQ 250 260 270 280 290 HSNDKVYSFY MMAPAGFAVE LGFGGQVIGD LESWHVGFYD APSIWGHELQ LPAH

References

[1]	"Characterization of 2,2',3-trihydroxybiphenyl dioxygenase, an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1." Happe B., Eltis L.D., Poth H., Hedderich R., Timmis K.N. J. Bacteriol. 175:7313-7320(1993) [PubMed: 8226678] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-64. Strain: RW1.
[2]	Armengaud J. Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X72850 Genomic DNA. Translation: CAA51364.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHY based on UniProtKB P17297.
ModBase	Search...
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR000486. Xdiol_dOase_1_2. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
ProDom	PD002334. Gly_diox. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00082. EXTRADIOL_DIOXYGENAS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DBFB_PSEPA

Accession

Primary (citable) accession number: P47243

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	January 23, 2007
Last modified:	November 25, 2008
This is version 46 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)