P47235 (GYRA_CAMFE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
DNA gyrase subunit A
EC=5.99.1.3

Gene names

Name:

gyrA

Organism

Campylobacter fetus

Taxonomic identifier

196 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Epsilonproteobacteria › Campylobacterales › Campylobacteraceae › Campylobacter

Protein attributes

Sequence length	862 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA gyrase negatively supercoils closed circular double-stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings. HAMAP MF_01897
Catalytic activity	ATP-dependent breakage, passage and rejoining of double-stranded DNA. HAMAP MF_01897
Subunit structure	Made up of two chains. The A chain is responsible for DNA breakage and rejoining; the B chain catalyzes ATP hydrolysis. The enzyme forms an A2B2 tetramer.
Subcellular location	Cytoplasm Potential HAMAP MF_01897.
Sequence similarities	Belongs to the topoisomerase GyrA/ParC subunit family.

Ontologies

Keywords
Biological process	Antibiotic resistance
Cellular component	Cytoplasm
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Isomerase Topoisomerase
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro response to antibiotic Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase (ATP-hydrolyzing) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 862	862	DNA gyrase subunit A HAMAP MF_01897		PRO_0000145226
Sites
	Active site	126	1	O-(5'-phospho-DNA)-tyrosine intermediate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47235 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E450D81809A10C35	FASTA	862	95,821
	10 20 30 40 50 60 MEENIFSSNQ DIDAIDVEDS IKASYLDYSM SVIIGRALPD ARDGLKPVHR RILYAMNDLG 70 80 90 100 110 120 VGSRSPYKKS ARIVGDVIGK YHPHGDTAVY DALVRMAQNF SMRVPAVDGQ GNFGSVDGDG 130 140 150 160 170 180 AAAMRYTEAR MTVLAEELLR DLDKDTVDFI PNYDDSLSEP DVLPARVPNL LLNGSSGIAV 190 200 210 220 230 240 GMATNIPPHS LDELVNGLLT LLDDKEVGLE DIMTHIKGPD FPTGGIIFGK KGIIEAYKTG 250 260 270 280 290 300 RGRIKLRAKT HIEKKPNKDV IVVDELPYQV NKAKLHADIA DLVKEKLIDG ISEVRDESDR 310 320 330 340 350 360 DGIRLVIELK RDAMSEIVLN NLFKSTQMEV TFGVIMLAIN NKEPKVFSLL ELLKLFLNHR 370 380 390 400 410 420 KTVIIRRTIF ELQKARARAH ILEGLKIALD NIDAVINLIK TSADTNSARD GLMAKFGLSE 430 440 450 460 470 480 LQSNAILDMR LSKLTGLERE KLEAELKEIL ELIEKLDAIL KSETLIENII RDELLEIKSK 490 500 510 520 530 540 FKCPRITDIV DDYDDIDVED LIPNENMVVT ITHRGYIKRV PSKSYEKQKR GGKGKVAVTT 550 560 570 580 590 600 YDDDFIESFF TCMSHDTLMF VTDRGQLYWL KVYKIPEGSR TAKGKAVVNL ISLQADEKIK 610 620 630 640 650 660 AIIPTTDFDE SKSLAFFTKN GIVKRTNLSE FKNIRSIGVK AINLDDNDEL VTVVIANSEP 670 680 690 700 710 720 DESYDDSFED GEGVSNLQTI SEDNSENSLE SGKMLFAVTK KGMCIKFALN KVRQIGRVSR 730 740 750 760 770 780 GVTAIRFKEN LDEVVGAVVI ENDSQEILSV SQKGIGKRTT ADEYRLQSRG GKGVICMKLT 790 800 810 820 830 840 PKTKDLVGVV MVDEEMDLMA LTSSGKMIRV DMQSIRKAGR NTSGVIVVNV DGDEVVSIAR 850 860 CPKEESDDDD IVADDTQEQD ME

References

[1]	"Cloning and nucleotide sequence of the gyrA gene from Campylobacter fetus subsp. fetus ATCC 27374 and characterization of ciprofloxacin-resistant laboratory and clinical isolates." Taylor D.E., Chau A.S. Antimicrob. Agents Chemother. 41:665-671(1997) [PubMed: 9056011] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27374 / UA60.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U25640 Genomic DNA. Translation: AAA67068.1.
3D structure databases
ProteinModelPortal	P47235.
SMR	P47235. Positions 34-489.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
PhylomeDB	P47235.
Family and domain databases
HAMAP	MF_01897. GyrA. [Tree]
InterPro	IPR005743. GyrA. IPR006691. GyrA/parC_pinwhl. IPR002205. Topo_IIA_A/C. IPR013758. Topo_IIA_A/C_ab. IPR013757. Topo_IIA_A_a. IPR013760. Topo_IIA_cen. [Graphical view]
Gene3D	G3DSA:3.90.199.10. Topo_IIA_A/C_ab. 1 hit. G3DSA:1.10.268.10. Topo_IIA_A_a. 1 hit.
Pfam	PF03989. DNA_gyraseA_C. 6 hits. PF00521. DNA_topoisoIV. 1 hit. [Graphical view]
SMART	SM00434. TOP4c. 1 hit. [Graphical view]
SUPFAM	SSF56719. Topo_IIA_cen. 1 hit.
TIGRFAMs	TIGR01063. GyrA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GYRA_CAMFE

Accession

Primary (citable) accession number: P47235

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 25, 2012
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program