Reviewed, UniProtKB/Swiss-Prot P47205 (LPXC_PSEAE)

Names and origin

Protein names

Recommended name:
    UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase
    EC=3.5.1.-
Alternative name(s):
    UDP-3-O-acyl-GlcNAc deacetylase
    Protein envA

Gene names

Name:	lpxC
Synonyms:	envA
Ordered Locus Names:	PA4406

Organism

Pseudomonas aeruginosa [Complete proteome] [HAMAP]

Taxonomic identifier

287 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Pseudomonas

Protein attributes

Sequence length	303 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Involved in the biosynthesis of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. HAMAP MF_00388
Catalytic activity	UDP-3-O-(3-hydroxytetradecanoyl)-N-acetylglucosamine + H2O = UDP-3-O-(3-hydroxytetradecanoyl)-glucosamine + acetate. HAMAP MF_00388
Pathway	Glycolipid biosynthesis; lipid (IV)A biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-D-glucosamine: step 2/6. HAMAP MF_00388
Sequence similarities	Belongs to the lpxC family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid synthesis
Molecular function	Hydrolase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 303

303

UDP-3-O-[3-hydroxymyristoyl] N-acetylglucosamine deacetylase HAMAP MF_00388

PRO_0000191945

Secondary structure

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303

Helix Strand Turn

Details...

Beta strand

3 – 9

7

Beta strand

11 – 16

6

Turn

18 – 20

3

Beta strand

22 – 30

9

Beta strand

37 – 41

5

Beta strand

44 – 46

3

Beta strand

48 – 51

4

Helix

54 – 56

3

Beta strand

61 – 63

3

Beta strand

66 – 68

3

Beta strand

71 – 74

4

Helix

77 – 86

10

Beta strand

91 – 99

9

Beta strand

105 – 107

3

Helix

108 – 117

10

Beta strand

119 – 125

7

Beta strand

128 – 131

4

Beta strand

135 – 139

5

Beta strand

142 – 147

6

Beta strand

150 – 157

8

Turn

163 – 167

5

Beta strand

171 – 175

5

Turn

176 – 178

3

Helix

181 – 184

4

Turn

185 – 187

3

Beta strand

191 – 193

3

Helix

194 – 202

9

Beta strand

213 – 217

5

Beta strand

219 – 222

4

Helix

233 – 246

14

Helix

247 – 249

3

Beta strand

250 – 261

12

Helix

264 – 276

13

Helix

278 – 280

3

Beta strand

281 – 284

4

Helix

289 – 291

3

Sequences

	Sequence		Length	Mass (Da)
	P47205-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 454403711DCC9B24	FASTA	303	33,435
	10 20 30 40 50 60 MIKQRTLKNI IRATGVGLHS GEKVYLTLKP APVDTGIVFC RTDLDPVVEI PARAENVGET 70 80 90 100 110 120 TMSTTLVKGD VKVDTVEHLL SAMAGLGIDN AYVELSASEV PIMDGSAGPF VFLIQSAGLQ 130 140 150 160 170 180 EQEAAKKFIR IKREVSVEEG DKRAVFVPFD GFKVSFEIDF DHPVFRGRTQ QASVDFSSTS 190 200 210 220 230 240 FVKEVSRART FGFMRDIEYL RSQNLALGGS VENAIVVDEN RVLNEDGLRY EDEFVKHKIL 250 260 270 280 290 300 DAIGDLYLLG NSLIGEFRGF KSGHALNNQL LRTLIADKDA WEVVTFEDAR TAPISYMRPA AAV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Levesque R.C. Submitted (JAN-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[2]	"Cloning, expression, and purification of UDP-3-O-acyl-GlcNAc deacetylase from Pseudomonas aeruginosa: a metalloamidase of the lipid A biosynthesis pathway." Hyland S.A., Eveland S.S., Anderson M.S. J. Bacteriol. 179:2029-2037(1997) [PubMed: 9068651] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 27853 / DSM 1117 / LMG 6395 / NCIMB 12469.
[3]	"Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic pathogen." Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P., Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M., Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y., Brody L.L., Coulter S.N., Folger K.R. , Kas A., Larbig K., Lim R.M., Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J., Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V. Nature 406:959-964(2000) [PubMed: 10984043] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
[4]	"Physical mapping of 32 genetic markers on the Pseudomonas aeruginosa PAO1 chromosome." Liao X., Charlebois I., Ouellet C., Morency M.J., Dewar K., Lightfoot J., Foster J., Siehnel R., Schweizer H., Lam J.S., Hancock R.E., Levesque R.C. Microbiology 142:79-86(1996) [PubMed: 8581173] [Abstract] Cited for: PRELIMINARY NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-42. Strain: ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

U19797 Genomic DNA. Translation: AAA95994.2.
U67855 Genomic DNA. Translation: AAC44974.1.
AE004091 Genomic DNA. Translation: AAG07794.1.

PIR

G83093.

RefSeq

NP_253096.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2VES	X-ray	1.90	A/B/C	1-299	[»]

ModBase

Search...

Genome annotation databases

GeneID

881292.

GenomeReviews

Gene locus PA4406 in contig AE004091_GR.

KEGG

pae:PA4406.

Organism-specific databases

PseudoCAP

PA4406.

CMR

Search...

Phylogenomic databases

HOGENOM

P47205.

OMA

P47205. TGVGVHS.

Enzyme and pathway databases

BioCyc

PAER208964:PA4406-MON.

Family and domain databases

HAMAP

MF_00388.
[Tree]

InterPro

IPR004463. UDP-acyl_GlcNac_deAcase.
IPR011334. UDP-acyl_GlcNac_deAcase_C.
IPR015870. UDP-acyl_N-AcGlcN_deAcase_N.
[Graphical view]

Gene3D

G3DSA:3.30.1700.10. UDP-acyl_N-AcGlcN_deAcase_C. 1 hit.
G3DSA:3.30.230.20. UDP-acyl_N-AcGlcN_deAcase_N. 1 hit.

Pfam

PF03331. LpxC. 1 hit.
[Graphical view]

ProDom

PD011499. Lipid_A_LpxC. 1 hit.
[Graphical view] [Entries sharing at least one domain]

TIGRFAMs

TIGR00325. lpxC. 1 hit.

ProtoNet

Search...

Entry information

Entry name

LPXC_PSEAE

Accession

Primary (citable) accession number: P47205
Secondary accession number(s): P97050

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	December 8, 2000
Last modified:	June 16, 2009
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)