Reviewed,
UniProtKB/Swiss-Prot P47197 (AKT2_RAT)
Last modified
November 25, 2008.
Version 67.
History...
Clusters with 100%,
90%,
50% identity |
Documents (1) |
Third-party data |
Customize display | text xml rdf/xml gff fasta |
Names and origin
| Protein names | Recommended name: RAC-beta serine/threonine-protein kinase EC=2.7.11.1 Alternative name(s): RAC-PK-beta Protein kinase Akt-2 Protein kinase B, beta Short name=PKB beta | ||
| Gene names |
| ||
| Organism | Rattus norvegicus (Rat) | ||
| Taxonomic identifier | 10116 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus |
Protein attributes
| Sequence length | 481 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is not processed. |
| Protein existence | Evidence at transcript level. |
General annotation (Comments)
| Function | General protein kinase capable of phosphorylating several known proteins. |
| Catalytic activity | ATP + a protein = ADP + a phosphoprotein. |
| Enzyme regulation | Two specific sites, one in the kinase domain (Thr-309) and the other in the C-terminal regulatory region (Ser-474), need to be phosphorylated for its full activation By similarity. |
| Subunit structure | Interacts (via PH domain) with MTCP1, TCL1A AND TCL1B By similarity. |
| Sequence similarities | Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. Contains 1 AGC-kinase C-terminal domain. Contains 1 PH domain. Contains 1 protein kinase domain. |
Ontologies
Keywords | |
|---|---|
| Ligand | ATP-binding Nucleotide-binding |
| Molecular function | Kinase Serine/threonine-protein kinase Transferase |
| PTM | Phosphoprotein |
Gene Ontology (GO) | |
| Biological process | protein amino acid phosphorylation Inferred from electronic annotation. Source: InterPro |
| Cellular component | cytosol Inferred from Experiment. Source: Reactome nucleoplasmInferred from Experiment. Source: Reactome |
| Molecular function | ATP binding Inferred from electronic annotation. Source: InterPro protein serine/threonine kinase activityInferred from electronic annotation. Source: InterPro |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 481 | 481 | RAC-beta serine/threonine-protein kinase | PRO_0000085610 | |||||
Regions | |||||||||
| Domain | 5 – 108 | 104 | PH | ||||||
| Domain | 152 – 409 | 258 | Protein kinase | ||||||
| Domain | 410 – 481 | 72 | AGC-kinase C-terminal | ||||||
| Nucleotide binding | 158 – 166 | 9 | ATP By similarity | ||||||
Sites | |||||||||
| Active site | 275 | 1 | Proton acceptor By similarity | ||||||
| Binding site | 181 | 1 | ATP By similarity | ||||||
Amino acid modifications | |||||||||
| Modified residue | 126 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 309 | 1 | Phosphothreonine; by PDPK1 By similarity | ||||||
| Modified residue | 474 | 1 | Phosphoserine By similarity | ||||||
Sequences
| ||||||||||||||||||
References
| [1] | "Molecular cloning of rat RAC protein kinase alpha and beta and their association with protein kinase C zeta." Konishi H., Shinomura T., Kuroda S.I., Ono Y., Kikkawa U. Biochem. Biophys. Res. Commun. 205:817-825(1994) [PubMed: 7999118] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Testis. |
Cross-references
Sequence databases | |
|---|---|
| D30041 mRNA. Translation: BAA06280.1. | |
| PIR | JC2438. |
| RefSeq | NP_058789.1. |
| UniGene | Rn.87066 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1GZK based on UniProtKB P31751. |
| SMR | P47197. Positions 1-111. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P47197. |
Genome annotation databases | |
| Ensembl | ENSRNOG00000018677. Rattus norvegicus. [Contig view] |
| GeneID | 25233. |
| KEGG | rno:25233. |
Organism-specific databases | |
| RGD | 2082. Akt2. |
Phylogenomic databases | |
| HOVERGEN | P47197. |
Gene expression databases | |
| ArrayExpress | P47197. |
| GermOnline | ENSRNOG00000018677. Rattus norvegicus. |
Family and domain databases | |
| InterPro | IPR015744. Akt. IPR001849. PH. IPR011993. PH_type. IPR000961. Pkinase_C. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_bd_CS. IPR017442. Se/Thr_pkinase-rel. IPR008271. Ser_thr_pkin_AS. IPR002290. Ser_thr_pkinase. [Graphical view] |
| Gene3D | G3DSA:2.30.29.30. PH_type. 1 hit. |
| PANTHER | PTHR22985:SF69. Akt. 1 hit. |
| Pfam | PF00169. PH. 1 hit. PF00069. Pkinase. 1 hit. PF00433. Pkinase_C. 1 hit. [Graphical view] |
| ProDom | PD000001. Prot_kinase. 1 hit. [Graphical view] [Entries sharing at least one domain] |
| SMART | SM00233. PH. 1 hit. SM00133. S_TK_X. 1 hit. SM00220. S_TKc. 1 hit. [Graphical view] |
| PROSITE | PS51285. AGC_KINASE_CTER. 1 hit. PS50003. PH_DOMAIN. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| NextBio | 605799. |
Entry information
| Entry name | AKT2_RAT | ||||||||
| Accession | Primary (citable) accession number: P47197 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||

Clusters with


