Reviewed, UniProtKB/Swiss-Prot P47195 (C80A1_BERST)

Names and origin

Protein names

Recommended name:
    Berbamunine synthase
    EC=1.14.21.3
Alternative name(s):
    Cytochrome P450 80
    CYPLXXX
    (S)-N-methylcoclaurine oxidase [C-O phenol-coupling]

Gene names

Name:	CYP80A1
Synonyms:	CYP80

Organism

Berberis stolonifera (Barberry)

Taxonomic identifier

33814 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › Ranunculales › Berberidaceae › Berberis

Protein attributes

Sequence length	487 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Forms the bisbenzylisoquinoline alkaloid berbamunine by phenol oxidation of N-methylcoclaurine without the incorporation of oxygen into the product. Oxidatively couples either two molecules of (R)-N-methylcoclaurine to form the (R,R) dimer guattegaumerine or one molecule each of (R)- and (S)-N-methylcoclaurine to form the (R,S) dimer berbamunine.
Catalytic activity	(S)-N-methylcoclaurine + (R)-N-methylcoclaurine + NADPH + O2 = berbamunine + NADP+ + 2 H2O.
Cofactor	Heme group By similarity.
Pathway	Alkaloid biosynthesis; berbamunine biosynthesis; berbamunine from (R)-N-methylcoclaurine and (S)-N-methylcoclaurine: step 1/1.
Subcellular location	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence similarities	Belongs to the cytochrome P450 family.

Ontologies

Keywords
Biological process	Alkaloid metabolism
Cellular component	Endoplasmic reticulum Membrane Microsome
Ligand	Heme Iron Metal-binding NADP
Molecular function	Monooxygenase Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	alkaloid metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	berbamunine synthase activity Inferred from electronic annotation. Source: EC electron carrier activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 487	487	Berbamunine synthase		PRO_0000052156
Sites
	Metal binding	429	1	Iron (heme axial ligand) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47195-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: AB2CBE4FFD26C672	FASTA	487	55,539
	10 20 30 40 50 60 MDYIVGFVSI SLVALLYFLL FKPKHTNLPP SPPAWPIVGH LPDLISKNSP PFLDYMSNIA 70 80 90 100 110 120 QKYGPLIHLK FGLHSSIFAS TKEAAMEVLQ TNDKVLSGRQ PLPCFRIKPH IDYSILWSDS 130 140 150 160 170 180 NSYWKKGRKI LHTEIFSQKM LQAQEKNRER VAGNLVNFIM TKVGDVVELR SWLFGCALNV 190 200 210 220 230 240 LGHVVFSKDV FEYSDQSDEV GMDKLIHGML MTGGDFDVAS YFPVLARFDL HGLKRKMDEQ 250 260 270 280 290 300 FKLLIKIWEG EVLARRANRN PEPKDMLDVL IANDFNEHQI NAMFMETFGP GSDTNSNIIE 310 320 330 340 350 360 WALAQLIKNP DKLAKLREEL DRVVGRSSTV KESHFSELPY LQACVKETMR LYPPISIMIP 370 380 390 400 410 420 HRCMETCQVM GYTIPKGMDV HVNAHAIGRD PKDWKDPLKF QPERFLDSDI EYNGKQFQFI 430 440 450 460 470 480 PFGSGRRICP GRPLAVRIIP LVLASLVHAF GWELPDGVPN EKLDMEELFT LSLCMAKPLR VIPKVRI

References

[1]

"Molecular cloning and heterologous expression of a cDNA encoding berbamunine synthase, a C-O phenol-coupling cytochrome P450 from the higher plant Berberis stolonifera." Kraus P.F.X., Kutchan T.M. Proc. Natl. Acad. Sci. U.S.A. 92:2071-2075(1995) [PubMed: 7892226] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 1-24. Strain: WOLF V29.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09610 mRNA. Translation: AAC48987.1.
3D structure databases
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.14.21.3. 9723.
Family and domain databases
InterPro	IPR001128. Cyt_P450. IPR017973. Cyt_P450_C. IPR017972. Cyt_P450_CS. IPR002401. Cyt_P450_E_grp-I. [Graphical view]
PANTHER	PTHR19383. Cyt_P450. 1 hit.
Pfam	PF00067. p450. 1 hit. [Graphical view]
PRINTS	PR00463. EP450I. PR00385. P450.
PROSITE	PS00086. CYTOCHROME_P450. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

C80A1_BERST

Accession

Primary (citable) accession number: P47195

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)