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Protein

Berbamunine synthase

Gene

CYP80A1

Organism
Berberis stolonifera (Barberry)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Forms the bisbenzylisoquinoline alkaloid berbamunine by phenol oxidation of N-methylcoclaurine without the incorporation of oxygen into the product. Oxidatively couples either two molecules of (R)-N-methylcoclaurine to form the (R,R) dimer guattegaumerine or one molecule each of (R)- and (S)-N-methylcoclaurine to form the (R,S) dimer berbamunine.

Catalytic activityi

(S)-N-methylcoclaurine + (R)-N-methylcoclaurine + NADPH + O2 = berbamunine + NADP+ + 2 H2O.

Cofactori

hemeBy similarity

Pathwayi: berbamunine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes berbamunine from (R)-N-methylcoclaurine and (S)-N-methylcoclaurine.
Proteins known to be involved in this subpathway in this organism are:
  1. Berbamunine synthase (CYP80A1)
This subpathway is part of the pathway berbamunine biosynthesis, which is itself part of Alkaloid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes berbamunine from (R)-N-methylcoclaurine and (S)-N-methylcoclaurine, the pathway berbamunine biosynthesis and in Alkaloid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi429Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Alkaloid metabolism

Keywords - Ligandi

Heme, Iron, Metal-binding, NADP

Enzyme and pathway databases

UniPathwayiUPA00308; UER00446.

Names & Taxonomyi

Protein namesi
Recommended name:
Berbamunine synthase (EC:1.14.21.3)
Alternative name(s):
(S)-N-methylcoclaurine oxidase [C-O phenol-coupling]
CYPLXXX
Cytochrome P450 80
Gene namesi
Name:CYP80A1
Synonyms:CYP80
OrganismiBerberis stolonifera (Barberry)
Taxonomic identifieri33814 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsRanunculalesBerberidaceaeBerberidoideaeBerberis

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000521561 – 487Berbamunine synthaseAdd BLAST487

Structurei

3D structure databases

ProteinModelPortaliP47195.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the cytochrome P450 family.Curated

Phylogenomic databases

KOiK13387.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47195-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYIVGFVSI SLVALLYFLL FKPKHTNLPP SPPAWPIVGH LPDLISKNSP
60 70 80 90 100
PFLDYMSNIA QKYGPLIHLK FGLHSSIFAS TKEAAMEVLQ TNDKVLSGRQ
110 120 130 140 150
PLPCFRIKPH IDYSILWSDS NSYWKKGRKI LHTEIFSQKM LQAQEKNRER
160 170 180 190 200
VAGNLVNFIM TKVGDVVELR SWLFGCALNV LGHVVFSKDV FEYSDQSDEV
210 220 230 240 250
GMDKLIHGML MTGGDFDVAS YFPVLARFDL HGLKRKMDEQ FKLLIKIWEG
260 270 280 290 300
EVLARRANRN PEPKDMLDVL IANDFNEHQI NAMFMETFGP GSDTNSNIIE
310 320 330 340 350
WALAQLIKNP DKLAKLREEL DRVVGRSSTV KESHFSELPY LQACVKETMR
360 370 380 390 400
LYPPISIMIP HRCMETCQVM GYTIPKGMDV HVNAHAIGRD PKDWKDPLKF
410 420 430 440 450
QPERFLDSDI EYNGKQFQFI PFGSGRRICP GRPLAVRIIP LVLASLVHAF
460 470 480
GWELPDGVPN EKLDMEELFT LSLCMAKPLR VIPKVRI
Length:487
Mass (Da):55,539
Last modified:November 1, 1995 - v1
Checksum:iAB2CBE4FFD26C672
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09610 mRNA. Translation: AAC48987.1.

Genome annotation databases

KEGGiag:AAC48987.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09610 mRNA. Translation: AAC48987.1.

3D structure databases

ProteinModelPortaliP47195.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

KEGGiag:AAC48987.

Phylogenomic databases

KOiK13387.

Enzyme and pathway databases

UniPathwayiUPA00308; UER00446.

Family and domain databases

Gene3Di1.10.630.10. 1 hit.
InterProiIPR001128. Cyt_P450.
IPR017972. Cyt_P450_CS.
IPR002401. Cyt_P450_E_grp-I.
[Graphical view]
PfamiPF00067. p450. 1 hit.
[Graphical view]
PRINTSiPR00463. EP450I.
PR00385. P450.
SUPFAMiSSF48264. SSF48264. 1 hit.
PROSITEiPS00086. CYTOCHROME_P450. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiC80A1_BERST
AccessioniPrimary (citable) accession number: P47195
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 5, 2016
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.