SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P47190

- PMT3_YEAST

UniProt

P47190 - PMT3_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Dolichyl-phosphate-mannose--protein mannosyltransferase 3
Gene
PMT3, YOR321W, O6148
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins By similarity.

Catalytic activityi

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

GO - Molecular functioni

  1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: SGD
  2. protein binding Source: IntAct

GO - Biological processi

  1. protein O-linked glycosylation Source: SGD
  2. protein O-linked mannosylation Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YOR321W-MONOMER.
BRENDAi2.4.1.109. 984.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 3 (EC:2.4.1.109)
Gene namesi
Name:PMT3
Ordered Locus Names:YOR321W
ORF Names:O6148
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome XV

Organism-specific databases

SGDiS000005848. PMT3.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 5050Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei51 – 7121Helical; Reviewed prediction
Add
BLAST
Topological domaini72 – 14877Lumenal Reviewed prediction
Add
BLAST
Transmembranei149 – 16921Helical; Reviewed prediction
Add
BLAST
Topological domaini170 – 1745Cytoplasmic Reviewed prediction
Transmembranei175 – 19521Helical; Reviewed prediction
Add
BLAST
Topological domaini196 – 23540Lumenal Reviewed prediction
Add
BLAST
Transmembranei236 – 25621Helical; Reviewed prediction
Add
BLAST
Topological domaini257 – 28226Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei283 – 30321Helical; Reviewed prediction
Add
BLAST
Topological domaini304 – 602299Lumenal Reviewed prediction
Add
BLAST
Transmembranei603 – 62321Helical; Reviewed prediction
Add
BLAST
Topological domaini624 – 63916Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei640 – 66021Helical; Reviewed prediction
Add
BLAST
Topological domaini661 – 6655Lumenal Reviewed prediction
Transmembranei666 – 68621Helical; Reviewed prediction
Add
BLAST
Topological domaini687 – 70317Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei704 – 72421Helical; Reviewed prediction
Add
BLAST
Topological domaini725 – 75329Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt3p dimer complex Source: SGD
  2. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt3p dimer complex Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 753753Dolichyl-phosphate-mannose--protein mannosyltransferase 3
PRO_0000121493Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi124 – 1241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi324 – 3241N-linked (GlcNAc...) Reviewed prediction
Glycosylationi398 – 3981N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Glycoprotein

Proteomic databases

MaxQBiP47190.
PaxDbiP47190.

Expressioni

Gene expression databases

GenevestigatoriP47190.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PMT1P337753EBI-13579,EBI-13567

Protein-protein interaction databases

BioGridi34707. 24 interactions.
DIPiDIP-5158N.
IntActiP47190. 4 interactions.
MINTiMINT-574047.
STRINGi4932.YOR321W.

Structurei

3D structure databases

ProteinModelPortaliP47190.
SMRiP47190. Positions 351-504.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini332 – 38756MIR 1
Add
BLAST
Domaini401 – 45757MIR 2
Add
BLAST
Domaini465 – 52359MIR 3
Add
BLAST

Sequence similaritiesi

Contains 3 MIR domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1928.
GeneTreeiENSGT00740000115531.
HOGENOMiHOG000157526.
KOiK00728.
OMAiFAAHFHI.
OrthoDBiEOG7BP89X.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47190-1 [UniParc]FASTAAdd to Basket

« Hide

MPYRVATGYS EKSTDDDLIW RTPIVKEELE DADNFLKDDA ELYDKVKNES    50
AVSHLDTIVM PIIFTVLGMF TRMYKIGRNN HVVWDEAHFG KFGSYYLRHE 100
FYHDVHPPLG KMLVGLSGYL AGYNGSWDFP SGEVYPDYID YVKMRLFQAM 150
FSSLCVPLAY FTGRAIGFSR LSVWLFTILV IFENSYATLG KFILLDSMLL 200
FFTVSSYFCL AKFHTMRKSP FSARWWLWLC LTGLNLGCAI SVKMVGLFII 250
SVVGIYTISE LWNLLSDRSV SWKVYVNHWL ARIFGLIIIP VCVFLLCFKI 300
HFDLLSNSGP GDSTMPSLFQ ASLNGTKVGK GPRDVALGSS IISIKNQALG 350
GALLHSHVQP FPEGSEQQQV TVYGYSDANN EWFFQRIRGV EPWTDAENKT 400
IEFVKGGEMY RLMHRLTGKN LHTHEVPAPI SKSEYEVSAY GDVDLGDYKD 450
NWIIEIVEQV GEEDPTLLHP LSTSFRIKNS ILGCYLAQSG KHLPEWGFRQ 500
GEVVCLKHAS KRDKRTWWNI ETHENERLPQ GEDFVYPKTS FFRNFMQLNS 550
AMMATNNALV PNPEKFDGIA SSAWQWPTLN VGVRLCEWSE KSIKYFLLGS 600
PASVWPSSIA VCALIIHVIF LTLKWQRQCV ILSDPVERDV FVMAAFYPLL 650
AWLLHYMPFV VMSRVVYAHH YLPTLYFALM ILSYYFDMIT KRWATRNTGK 700
FLRLGAYIVY GSIVIAGFFY FSPFSFGMDG PVDDYAYLAW LPTWQIVEDI 750
RNT 753
Length:753
Mass (Da):86,323
Last modified:October 5, 2010 - v2
Checksum:iF076473B41EB6CBA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti397 – 3971E → H in CAA58728. 1 Publication
Sequence conflicti567 – 5671D → N in CAA58728. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83797 Genomic DNA. Translation: CAA58728.1.
X90565 Genomic DNA. Translation: CAA62176.1.
Z75229 Genomic DNA. Translation: CAA99641.1.
BK006948 Genomic DNA. Translation: DAA11085.1.
PIRiS58331.
RefSeqiNP_014966.1. NM_001183741.1.

Genome annotation databases

EnsemblFungiiYOR321W; YOR321W; YOR321W.
GeneIDi854499.
KEGGisce:YOR321W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X83797 Genomic DNA. Translation: CAA58728.1 .
X90565 Genomic DNA. Translation: CAA62176.1 .
Z75229 Genomic DNA. Translation: CAA99641.1 .
BK006948 Genomic DNA. Translation: DAA11085.1 .
PIRi S58331.
RefSeqi NP_014966.1. NM_001183741.1.

3D structure databases

ProteinModelPortali P47190.
SMRi P47190. Positions 351-504.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 34707. 24 interactions.
DIPi DIP-5158N.
IntActi P47190. 4 interactions.
MINTi MINT-574047.
STRINGi 4932.YOR321W.

Protein family/group databases

CAZyi GT39. Glycosyltransferase Family 39.

Proteomic databases

MaxQBi P47190.
PaxDbi P47190.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YOR321W ; YOR321W ; YOR321W .
GeneIDi 854499.
KEGGi sce:YOR321W.

Organism-specific databases

SGDi S000005848. PMT3.

Phylogenomic databases

eggNOGi COG1928.
GeneTreei ENSGT00740000115531.
HOGENOMi HOG000157526.
KOi K00728.
OMAi FAAHFHI.
OrthoDBi EOG7BP89X.

Enzyme and pathway databases

BioCyci YEAST:YOR321W-MONOMER.
BRENDAi 2.4.1.109. 984.

Miscellaneous databases

NextBioi 976837.

Gene expression databases

Genevestigatori P47190.

Family and domain databases

InterProi IPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view ]
PANTHERi PTHR10050. PTHR10050. 1 hit.
Pfami PF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view ]
SMARTi SM00472. MIR. 3 hits.
[Graphical view ]
SUPFAMi SSF82109. SSF82109. 1 hit.
PROSITEi PS50919. MIR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "PMT3 and PMT4, two new members of the protein-O-mannosyltransferase gene family of Saccharomyces cerevisiae."
    Immervoll T., Gentzsch M., Tanner W.
    Yeast 11:1345-1351(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Sequencing of a 35.71 kb DNA segment on the right arm of yeast chromosome XV reveals regions of similarity to chromosomes I and XIII."
    Pearson B.M., Hernando Y., Payne J., Wolf S.S., Kalogeropoulos A., Schweizer M.
    Yeast 12:1021-1031(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 96604 / S288c / FY1679.
  3. "The nucleotide sequence of Saccharomyces cerevisiae chromosome XV."
    Dujon B., Albermann K., Aldea M., Alexandraki D., Ansorge W., Arino J., Benes V., Bohn C., Bolotin-Fukuhara M., Bordonne R., Boyer J., Camasses A., Casamayor A., Casas C., Cheret G., Cziepluch C., Daignan-Fornier B., Dang V.-D.
    , de Haan M., Delius H., Durand P., Fairhead C., Feldmann H., Gaillon L., Galisson F., Gamo F.-J., Gancedo C., Goffeau A., Goulding S.E., Grivell L.A., Habbig B., Hand N.J., Hani J., Hattenhorst U., Hebling U., Hernando Y., Herrero E., Heumann K., Hiesel R., Hilger F., Hofmann B., Hollenberg C.P., Hughes B., Jauniaux J.-C., Kalogeropoulos A., Katsoulou C., Kordes E., Lafuente M.J., Landt O., Louis E.J., Maarse A.C., Madania A., Mannhaupt G., Marck C., Martin R.P., Mewes H.-W., Michaux G., Paces V., Parle-McDermott A.G., Pearson B.M., Perrin A., Pettersson B., Poch O., Pohl T.M., Poirey R., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rechmann S., Schwager C., Schweizer M., Sor F., Sterky F., Tarassov I.A., Teodoru C., Tettelin H., Thierry A., Tobiasch E., Tzermia M., Uhlen M., Unseld M., Valens M., Vandenbol M., Vetter I., Vlcek C., Voet M., Volckaert G., Voss H., Wambutt R., Wedler H., Wiemann S., Winsor B., Wolfe K.H., Zollner A., Zumstein E., Kleine K.
    Nature 387:98-102(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.

Entry informationi

Entry nameiPMT3_YEAST
AccessioniPrimary (citable) accession number: P47190
Secondary accession number(s): D6W319
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: October 5, 2010
Last modified: June 11, 2014
This is version 123 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2720 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome XV
    Yeast (Saccharomyces cerevisiae) chromosome XV: entries and gene names

External Data

Dasty 3

Similar proteinsi