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Protein

Polygalacturonase

Gene

PGU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei200Proton donorBy similarity1
Active sitei222PROSITE-ProRule annotation1

GO - Molecular functioni

  • polygalacturonase activity Source: SGD

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: SGD
  • pseudohyphal growth Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:YJR153W-MONOMER.
BRENDAi3.2.1.15. 984.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28A_YEAST.

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
PGase SM
Pectinase
Gene namesi
Name:PGU1
Synonyms:PGL1, PSM1
Ordered Locus Names:YJR153W
ORF Names:J2235
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR153W.
SGDiS000003914. PGU1.

Subcellular locationi

GO - Cellular componenti

  • extracellular region Source: SGD

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002479719 – 361PolygalacturonaseAdd BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 43By similarity
Disulfide bondi202 ↔ 218By similarity
Glycosylationi318N-linked (GlcNAc...)PROSITE-ProRule annotation1
Glycosylationi330N-linked (GlcNAc...)PROSITE-ProRule annotation1
Disulfide bondi350 ↔ 361By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP47180.

Interactioni

Protein-protein interaction databases

BioGridi33908. 32 interactors.

Structurei

3D structure databases

ProteinModelPortaliP47180.
SMRiP47180.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati155 – 185PbH1 1Sequence analysisAdd BLAST31
Repeati186 – 207PbH1 2Sequence analysisAdd BLAST22
Repeati208 – 228PbH1 3Sequence analysisAdd BLAST21
Repeati237 – 258PbH1 4Sequence analysisAdd BLAST22
Repeati266 – 288PbH1 5Sequence analysisAdd BLAST23

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000193107.
InParanoidiP47180.
KOiK01184.
OMAiVQGFSIQ.
OrthoDBiEOG092C33Y3.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiView protein in InterPro
IPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
PfamiView protein in Pfam
PF00295. Glyco_hydro_28. 1 hit.
SMARTiView protein in SMART
SM00710. PbH1. 5 hits.
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiView protein in PROSITE
PS00502. POLYGALACTURONASE. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISANSLLIS TLCAFAIATP LSKRDSCTLT GSSLSSLSTV KKCSSIVIKD
60 70 80 90 100
LTVPAGQTLD LTGLSSGTTV TFEGTTTFQY KEWSGPLISI SGSKISVVGA
110 120 130 140 150
SGHTIDGQGA KWWDGLGDSG KVKPKFVKLA LTGTSKVTGL NIKNAPHQVF
160 170 180 190 200
SINKCSDLTI SDITIDIRDG DSAGGHNTDG FDVGSSSNVL IQGCTVYNQD
210 220 230 240 250
DCIAVNSGST IKFMNNYCYN GHGISVGSVG GRSDNTVNGF WAENNHVINS
260 270 280 290 300
DNGLRIKTVE GATGTVTNVN FISNKISGIK SYGIVIEGDY LNSKTTGTAT
310 320 330 340 350
GGVPISNLVM KDITGSVNST AKRVKILVKN ATNWQWSGVS ITGGSSYSGC
360
SGIPSGSGAS C
Length:361
Mass (Da):37,287
Last modified:November 1, 1995 - v1
Checksum:iA67798529BFB2EFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23K → R in AAS56418 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011818 Genomic DNA. Translation: BAA25103.1.
Z49653 Genomic DNA. Translation: CAA89686.1.
AY558092 Genomic DNA. Translation: AAS56418.1.
BK006943 Genomic DNA. Translation: DAA08938.1.
PIRiS57182.
RefSeqiNP_012687.3. NM_001181811.3.

Genome annotation databases

EnsemblFungiiYJR153W; YJR153W; YJR153W.
GeneIDi853618.
KEGGisce:YJR153W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011818 Genomic DNA. Translation: BAA25103.1.
Z49653 Genomic DNA. Translation: CAA89686.1.
AY558092 Genomic DNA. Translation: AAS56418.1.
BK006943 Genomic DNA. Translation: DAA08938.1.
PIRiS57182.
RefSeqiNP_012687.3. NM_001181811.3.

3D structure databases

ProteinModelPortaliP47180.
SMRiP47180.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33908. 32 interactors.

Protein family/group databases

CAZyiGH28. Glycoside Hydrolase Family 28.
mycoCLAPiPGA28A_YEAST.

Proteomic databases

PRIDEiP47180.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR153W; YJR153W; YJR153W.
GeneIDi853618.
KEGGisce:YJR153W.

Organism-specific databases

EuPathDBiFungiDB:YJR153W.
SGDiS000003914. PGU1.

Phylogenomic databases

HOGENOMiHOG000193107.
InParanoidiP47180.
KOiK01184.
OMAiVQGFSIQ.
OrthoDBiEOG092C33Y3.

Enzyme and pathway databases

BioCyciYEAST:YJR153W-MONOMER.
BRENDAi3.2.1.15. 984.

Miscellaneous databases

PROiP47180.

Family and domain databases

Gene3Di2.160.20.10. 1 hit.
InterProiView protein in InterPro
IPR000743. Glyco_hydro_28.
IPR006626. PbH1.
IPR012334. Pectin_lyas_fold.
IPR011050. Pectin_lyase_fold/virulence.
PfamiView protein in Pfam
PF00295. Glyco_hydro_28. 1 hit.
SMARTiView protein in SMART
SM00710. PbH1. 5 hits.
SUPFAMiSSF51126. SSF51126. 1 hit.
PROSITEiView protein in PROSITE
PS00502. POLYGALACTURONASE. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPGLR_YEAST
AccessioniPrimary (citable) accession number: P47180
Secondary accession number(s): D6VWX2, E9P8V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 15, 2017
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.