P47180 (PGLR_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Polygalacturonase
Short name=PG
EC=3.2.1.15

Alternative name(s):
PGase SM
Pectinase

Gene names

Name:	PGU1
Synonyms:	PGL1, PSM1
Ordered Locus Names:	YJR153W
ORF Names:	J2235

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	361 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.
Sequence similarities	Belongs to the glycosyl hydrolase 28 family. Contains 5 PbH1 repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Domain	Repeat Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW pectin catabolic process Inferred from direct assay. Source: SGD pseudohyphal growth Non-traceable author statement. Source: SGD
Cellular component	extracellular region Inferred from direct assay. Source: SGD
Molecular function	polygalacturonase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Chain	19 – 361	343	Polygalacturonase		PRO_0000024797
Sites
	Active site	200	1	Proton donor By similarity
	Active site	222	1	By similarity
Amino acid modifications
	Glycosylation	318	1	N-linked (GlcNAc...) Potential
	Glycosylation	330	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	23	1	K → R in AAS56418. Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P47180 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A67798529BFB2EFB	FASTA	361	37,287
	10 20 30 40 50 60 MISANSLLIS TLCAFAIATP LSKRDSCTLT GSSLSSLSTV KKCSSIVIKD LTVPAGQTLD 70 80 90 100 110 120 LTGLSSGTTV TFEGTTTFQY KEWSGPLISI SGSKISVVGA SGHTIDGQGA KWWDGLGDSG 130 140 150 160 170 180 KVKPKFVKLA LTGTSKVTGL NIKNAPHQVF SINKCSDLTI SDITIDIRDG DSAGGHNTDG 190 200 210 220 230 240 FDVGSSSNVL IQGCTVYNQD DCIAVNSGST IKFMNNYCYN GHGISVGSVG GRSDNTVNGF 250 260 270 280 290 300 WAENNHVINS DNGLRIKTVE GATGTVTNVN FISNKISGIK SYGIVIEGDY LNSKTTGTAT 310 320 330 340 350 360 GGVPISNLVM KDITGSVNST AKRVKILVKN ATNWQWSGVS ITGGSSYSGC SGIPSGSGAS C

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Endo-polygalacturonase gene." Hirose N., Kishida M., Kawasaki H., Sakai T. Submitted (MAR-1998) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SSM52.
[2]	"Cloning, molecular characterization, and expression of an endo-polygalacturonase-encoding gene from Saccharomyces cerevisiae IM1-8b." Blanco P., Sieiro C., Reboredo N.M., Villa T.G. FEMS Microbiol. Lett. 164:249-255(1998) [PubMed: 9682473] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Production and partial characterization of an endopolygalacturonase from Saccharomyces cerevisiae." Blanco P., Sieiro C., Diaz A., Villa T.G. Can. J. Microbiol. 40:974-977(1994) [PubMed: 7804908] [Abstract] Cited for: CHARACTERIZATION. Strain: CECT 1389.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AB011818 Genomic DNA. Translation: BAA25103.1. Z49653 Genomic DNA. Translation: CAA89686.1. AY558092 Genomic DNA. Translation: AAS56418.1. BK006943 Genomic DNA. Translation: DAA08938.1.
PIR	S57182.
RefSeq	NP_012687.1. NM_001181811.1.
3D structure databases
ProteinModelPortal	P47180.
SMR	P47180. Positions 25-359.
ModBase	Search...
Protein-protein interaction databases
STRING	P47180.
Protein family/group databases
CAZy	GH28. Glycoside Hydrolase Family 28.
Proteomic databases
PeptideAtlas	P47180.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR153W; YJR153W; YJR153W.
GeneID	853618.
KEGG	sce:YJR153W.
NMPDR	fig|4932.3.peg.3665.
Organism-specific databases
CYGD	YJR153w.
SGD	S000003914. PGU1.
Phylogenomic databases
eggNOG	fuNOG07644.
HOGENOM	HBG324519.
OMA	LAINSGT.
OrthoDB	EOG46DQBJ.
Gene expression databases
ArrayExpress	P47180.
Genevestigator	P47180.
GermOnline	YJR153W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000743. Glyco_hydro_28. IPR006626. PbH1. IPR012334. Pectin_lyas_fold. IPR011050. Pectin_lyase_fold/virulence. [Graphical view]
Gene3D	G3DSA:2.160.20.10. Pectin_lyas_fold. 1 hit.
KO	K01184.
Pfam	PF00295. Glyco_hydro_28. 1 hit. [Graphical view]
SMART	SM00710. PbH1. 5 hits. [Graphical view]
SUPFAM	SSF51126. Pectin_lyas_like. 1 hit.
PROSITE	PS00502. POLYGALACTURONASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974475.

Entry information

Entry name

PGLR_YEAST

Accession

Primary (citable) accession number: P47180
Secondary accession number(s): D6VWX2, E9P8V0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	December 14, 2011
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program