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Protein

Polygalacturonase

Gene

PGU1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

Random hydrolysis of (1->4)-alpha-D-galactosiduronic linkages in pectate and other galacturonans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei200Proton donorBy similarity1
Active sitei222PROSITE-ProRule annotation1

GO - Molecular functioni

  • polygalacturonase activity Source: SGD

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • pectin catabolic process Source: SGD
  • pseudohyphal growth Source: SGD

Keywordsi

Molecular functionGlycosidase, Hydrolase
Biological processCell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciYEAST:YJR153W-MONOMER
BRENDAi3.2.1.15 984

Protein family/group databases

CAZyiGH28 Glycoside Hydrolase Family 28
mycoCLAPiPGA28A_YEAST

Names & Taxonomyi

Protein namesi
Recommended name:
Polygalacturonase (EC:3.2.1.15)
Short name:
PG
Alternative name(s):
PGase SM
Pectinase
Gene namesi
Name:PGU1
Synonyms:PGL1, PSM1
Ordered Locus Names:YJR153W
ORF Names:J2235
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR153W
SGDiS000003914 PGU1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000002479719 – 361PolygalacturonaseAdd BLAST343

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi27 ↔ 43By similarity
Disulfide bondi202 ↔ 218By similarity
Glycosylationi318N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Glycosylationi330N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation1
Disulfide bondi350 ↔ 361By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP47180
PRIDEiP47180

Interactioni

Protein-protein interaction databases

BioGridi33908, 33 interactors
STRINGi4932.YJR153W

Structurei

3D structure databases

ProteinModelPortaliP47180
SMRiP47180
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati155 – 185PbH1 1Sequence analysisAdd BLAST31
Repeati186 – 207PbH1 2Sequence analysisAdd BLAST22
Repeati208 – 228PbH1 3Sequence analysisAdd BLAST21
Repeati237 – 258PbH1 4Sequence analysisAdd BLAST22
Repeati266 – 288PbH1 5Sequence analysisAdd BLAST23

Sequence similaritiesi

Belongs to the glycosyl hydrolase 28 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

HOGENOMiHOG000193107
InParanoidiP47180
KOiK01184
OMAiNTPVQGF
OrthoDBiEOG092C33Y3

Family and domain databases

Gene3Di2.160.20.10, 1 hit
InterProiView protein in InterPro
IPR000743 Glyco_hydro_28
IPR006626 PbH1
IPR012334 Pectin_lyas_fold
IPR011050 Pectin_lyase_fold/virulence
PfamiView protein in Pfam
PF00295 Glyco_hydro_28, 1 hit
SMARTiView protein in SMART
SM00710 PbH1, 5 hits
SUPFAMiSSF51126 SSF51126, 1 hit
PROSITEiView protein in PROSITE
PS00502 POLYGALACTURONASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47180-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISANSLLIS TLCAFAIATP LSKRDSCTLT GSSLSSLSTV KKCSSIVIKD
60 70 80 90 100
LTVPAGQTLD LTGLSSGTTV TFEGTTTFQY KEWSGPLISI SGSKISVVGA
110 120 130 140 150
SGHTIDGQGA KWWDGLGDSG KVKPKFVKLA LTGTSKVTGL NIKNAPHQVF
160 170 180 190 200
SINKCSDLTI SDITIDIRDG DSAGGHNTDG FDVGSSSNVL IQGCTVYNQD
210 220 230 240 250
DCIAVNSGST IKFMNNYCYN GHGISVGSVG GRSDNTVNGF WAENNHVINS
260 270 280 290 300
DNGLRIKTVE GATGTVTNVN FISNKISGIK SYGIVIEGDY LNSKTTGTAT
310 320 330 340 350
GGVPISNLVM KDITGSVNST AKRVKILVKN ATNWQWSGVS ITGGSSYSGC
360
SGIPSGSGAS C
Length:361
Mass (Da):37,287
Last modified:November 1, 1995 - v1
Checksum:iA67798529BFB2EFB
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti23K → R in AAS56418 (PubMed:17322287).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB011818 Genomic DNA Translation: BAA25103.1
Z49653 Genomic DNA Translation: CAA89686.1
AY558092 Genomic DNA Translation: AAS56418.1
BK006943 Genomic DNA Translation: DAA08938.1
PIRiS57182
RefSeqiNP_012687.3, NM_001181811.3

Genome annotation databases

EnsemblFungiiYJR153W; YJR153W; YJR153W
GeneIDi853618
KEGGisce:YJR153W

Similar proteinsi

Entry informationi

Entry nameiPGLR_YEAST
AccessioniPrimary (citable) accession number: P47180
Secondary accession number(s): D6VWX2, E9P8V0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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