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Protein

Cell wall protein DAN4

Gene

DAN4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Component of the cell wall.2 Publications

GO - Biological processi

Complete GO annotation...

Enzyme and pathway databases

BioCyciYEAST:G3O-31764-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell wall protein DAN4Curated
Alternative name(s):
Delayed anaerobic protein 41 Publication
Gene namesi
Name:DAN41 Publication
Ordered Locus Names:YJR151CImported
ORF Names:J2223Imported
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR151C.
SGDiS000003912. DAN4.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cell wall, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1919Sequence analysisAdd
BLAST
Chaini20 – 11371118Cell wall protein DAN4PRO_0000033243Add
BLAST
Propeptidei1138 – 116124Removed in mature formSequence analysisPRO_0000033244Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Lipidationi1137 – 11371GPI-anchor amidated asparagineSequence analysis

Post-translational modificationi

Extensively O-glycosylated.Curated
The GPI-anchor is attached to the protein in the endoplasmic reticulum and serves to target the protein to the cell surface. There, the glucosamine-inositol phospholipid moiety is cleaved off and the GPI-modified mannoprotein is covalently attached via its lipidless GPI glycan remnant to the 1,6-beta-glucan of the outer cell wall layer.Curated

Keywords - PTMi

Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Inductioni

Induced during anaerobic growth and completely repressed during aerobic growth.2 Publications

Interactioni

Protein-protein interaction databases

BioGridi33906. 23 interactions.

Structurei

3D structure databases

ProteinModelPortaliP47179.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati373 – 384121-11 PublicationAdd
BLAST
Repeati385 – 396121-21 PublicationAdd
BLAST
Repeati397 – 408121-31 PublicationAdd
BLAST
Repeati409 – 420121-41 PublicationAdd
BLAST
Repeati421 – 432121-51 PublicationAdd
BLAST
Repeati433 – 444121-61 PublicationAdd
BLAST
Repeati445 – 456121-71 PublicationAdd
BLAST
Repeati457 – 468121-81 PublicationAdd
BLAST
Repeati469 – 480121-91 PublicationAdd
BLAST
Repeati481 – 492121-101 PublicationAdd
BLAST
Repeati493 – 504121-111 PublicationAdd
BLAST
Repeati505 – 516121-121 PublicationAdd
BLAST
Repeati517 – 528121-131 PublicationAdd
BLAST
Repeati529 – 540121-141 PublicationAdd
BLAST
Repeati826 – 913882-11 PublicationAdd
BLAST
Repeati914 – 1001882-21 PublicationAdd
BLAST
Repeati1002 – 1040392-3; truncated1 PublicationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 28615346 X 3 AA tandem repeats of T-[SP]-TAdd
BLAST
Regioni373 – 54016814 X 12 AA approximate tandem repeatsAdd
BLAST
Regioni826 – 10402152.5 X 88 AA approximate tandem repeatsAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi124 – 794671Ser/Thr-richAdd
BLAST

Domaini

The number of the intragenic tandem repeats varies between different S.cerevisiae strains.1 Publication

Sequence similaritiesi

Belongs to the SRP1/TIP1 family.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

GeneTreeiENSGT00730000111524.
InParanoidiP47179.
OMAiPNVGQQF.
OrthoDBiEOG7NCVGR.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47179-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNISIVAGI VALATSAAAI TATTTLSPYD ERVNLIELAV YVSDIRAHIF
60 70 80 90 100
QYYSFRNHHK TETYPSEIAA AVFDYGDFTT RLTGISGDEV TRMITGVPWY
110 120 130 140 150
STRLKPAISS ALSKDGIYTA IPTSTSTTTT KSSTSTTPTT TITSTTSTTS
160 170 180 190 200
TTPTTSTTST TPTTSTTSTT PTTSTTSTTP TTSTTSTTPT TSTTSTTPTT
210 220 230 240 250
STTSTTPTTS TTSTTPTTST TSTTPTTSTT PTTSTTSTTS QTSTKSTTPT
260 270 280 290 300
TSSTSTTPTT STTPTTSTTS TAPTTSTTST TSTTSTISTA PTTSTTSSTF
310 320 330 340 350
STSSASASSV ISTTATTSTT FASLTTPATS TASTDHTTSS VSTTNAFTTS
360 370 380 390 400
ATTTTTSDTY ISSSSPSQVT SSAEPTTVSE VTSSVEPTRS SQVTSSAEPT
410 420 430 440 450
TVSEFTSSVE PTRSSQVTSS AEPTTVSEFT SSVEPTRSSQ VTSSAEPTTV
460 470 480 490 500
SEFTSSVEPT RSSQVTSSAE PTTVSEFTSS VEPTRSSQVT SSAEPTTVSE
510 520 530 540 550
FTSSVEPIRS SQVTSSAEPT TVSEVTSSVE PIRSSQVTTT EPVSSFGSTF
560 570 580 590 600
SEITSSAEPL SFSKATTSAE SISSNQITIS SELIVSSVIT SSSEIPSSIE
610 620 630 640 650
VLTSSGISSS VEPTSLVGPS SDESISSTES LSATSTFTSA VVSSSKAADF
660 670 680 690 700
FTRSTVSAKS DVSGNSSTQS TTFFATPSTP LAVSSTVVTS STDSVSPNIP
710 720 730 740 750
FSEISSSPES STAITSTSTS FIAERTSSLY LSSSNMSSFT LSTFTVSQSI
760 770 780 790 800
VSSFSMEPTS SVASFASSSP LLVSSRSNCS DARSSNTISS GLFSTIENVR
810 820 830 840 850
NATSTFTNLS TDEIVITSCK SSCTNEDSVL TKTQVSTVET TITSCSGGIC
860 870 880 890 900
TTLMSPVTTI NAKANTLTTT ETSTVETTIT TCPGGVCSTL TVPVTTITSE
910 920 930 940 950
ATTTATISCE DNEEDITSTE TELLTLETTI TSCSGGICTT LMSPVTTINA
960 970 980 990 1000
KANTLTTTET STVETTITTC SGGVCSTLTV PVTTITSEAT TTATISCEDN
1010 1020 1030 1040 1050
EEDVASTKTE LLTMETTITS CSGGICTTLM SPVSSFNSKA TTSNNAESTI
1060 1070 1080 1090 1100
PKAIKVSCSA GACTTLTTVD AGISMFTRTG LSITQTTVTN CSGGTCTMLT
1110 1120 1130 1140 1150
APIATATSKV ISPIPKASSA TSIAHSSASY TVSINTNGAY NFDKDNIFGT
1160
AIVAVVALLL L
Length:1,161
Mass (Da):118,360
Last modified:February 1, 1996 - v1
Checksum:i7954C15D69F0CA58
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49651 Genomic DNA. Translation: CAA89684.1.
BK006943 Genomic DNA. Translation: DAA08935.1.
PIRiS57180.
RefSeqiNP_012685.1. NM_001181809.2.

Genome annotation databases

EnsemblFungiiYJR151C; YJR151C; YJR151C.
GeneIDi853616.
KEGGisce:YJR151C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49651 Genomic DNA. Translation: CAA89684.1.
BK006943 Genomic DNA. Translation: DAA08935.1.
PIRiS57180.
RefSeqiNP_012685.1. NM_001181809.2.

3D structure databases

ProteinModelPortaliP47179.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33906. 23 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR151C; YJR151C; YJR151C.
GeneIDi853616.
KEGGisce:YJR151C.

Organism-specific databases

EuPathDBiFungiDB:YJR151C.
SGDiS000003912. DAN4.

Phylogenomic databases

GeneTreeiENSGT00730000111524.
InParanoidiP47179.
OMAiPNVGQQF.
OrthoDBiEOG7NCVGR.

Enzyme and pathway databases

BioCyciYEAST:G3O-31764-MONOMER.

Miscellaneous databases

PROiP47179.

Family and domain databases

InterProiIPR000992. SRP1_TIP1.
[Graphical view]
PfamiPF00660. SRP1_TIP1. 1 hit.
[Graphical view]
PROSITEiPS00724. SRP1_TIP1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Screening for glycosylphosphatidylinositol (GPI)-dependent cell wall proteins in Saccharomyces cerevisiae."
    Hamada K., Fukuchi S., Arisawa M., Baba M., Kitada K.
    Mol. Gen. Genet. 258:53-59(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION.
  4. "Reciprocal regulation of anaerobic and aerobic cell wall mannoprotein gene expression in Saccharomyces cerevisiae."
    Abramova N.E., Sertil O., Mehta S., Lowry C.V.
    J. Bacteriol. 183:2881-2887(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  5. "Induction and repression of DAN1 and the family of anaerobic mannoprotein genes in Saccharomyces cerevisiae occurs through a complex array of regulatory sites."
    Cohen B.D., Sertil O., Abramova N.E., Davies K.J.A., Lowry C.V.
    Nucleic Acids Res. 29:799-808(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  6. "Intragenic tandem repeats generate functional variability."
    Verstrepen K.J., Jansen A., Lewitter F., Fink G.R.
    Nat. Genet. 37:986-990(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: REPEATS.

Entry informationi

Entry nameiDAN4_YEAST
AccessioniPrimary (citable) accession number: P47179
Secondary accession number(s): D6VWW9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 112 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.