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P47177

- 2NDP_YEAST

UniProt

P47177 - 2NDP_YEAST

Protein

Putative nitronate monooxygenase

Gene

YJR149W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 2 (21 Sep 2011)
      Previous versions | rss
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    Functioni

    Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites.By similarity

    Catalytic activityi

    Ethylnitronate + O2 = acetaldehyde + nitrite + other products.

    Cofactori

    Binds 1 FMN per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei224 – 2241Proton acceptorSequence Analysis
    Binding sitei224 – 2241SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi41 – 433FMNBy similarity
    Nucleotide bindingi270 – 2723FMNBy similarity
    Nucleotide bindingi293 – 2942FMNBy similarity

    GO - Molecular functioni

    1. nitronate monooxygenase activity Source: UniProtKB-EC

    Keywords - Molecular functioni

    Monooxygenase, Oxidoreductase

    Keywords - Ligandi

    Flavoprotein, FMN

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31762-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Putative nitronate monooxygenase (EC:1.13.12.16)
    Alternative name(s):
    Nitroalkane oxidase
    Gene namesi
    Ordered Locus Names:YJR149W
    ORF Names:J2213
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR149w.
    SGDiS000003910. YJR149W.

    Subcellular locationi

    Cytoplasm 1 Publication

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 404404Putative nitronate monooxygenasePRO_0000203128Add
    BLAST

    Proteomic databases

    PaxDbiP47177.

    Expressioni

    Gene expression databases

    GenevestigatoriP47177.

    Interactioni

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    PRP40P332031EBI-25740,EBI-701
    RSP5P399401EBI-25740,EBI-16219

    Protein-protein interaction databases

    BioGridi33904. 8 interactions.
    IntActiP47177. 4 interactions.
    MINTiMINT-4083947.
    STRINGi4932.YJR149W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47177.
    SMRiP47177. Positions 169-399.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the nitronate monooxygenase family.Curated

    Phylogenomic databases

    eggNOGiCOG2070.
    HOGENOMiHOG000123287.
    KOiK00459.
    OMAiTQVGTMA.
    OrthoDBiEOG74N5SH.

    Family and domain databases

    Gene3Di3.20.20.70. 2 hits.
    InterProiIPR004136. 2Npropane_dOase.
    IPR013785. Aldolase_TIM.
    [Graphical view]
    PfamiPF03060. NMO. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47177-1 [UniParc]FASTAAdd to Basket

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    MYFLNQLIFQ DVSVMSVDKR EDMSRSFQKC LNLRYPIIQA PMAGVTTIEM    50
    AAKACIAGAI ASLPLSHLDF RKVNDIEKLK LMVSQFRDQV ADESLEGNLN 100
    LNFFCHDIVD KPTDLQTANW AKLYRKSMNV PIDMNEIKFD NGNVSFKAFE 150
    KENALQDFFQ YLSDGFRPKI ISFHFGHPSK STIEYLQKIG ILIFVTATSV 200
    REVRLLARLG INGIVCQGYE AGGHRGNFLV NDPKDDENLS TVQLVKRTVD 250
    ELAEMKNKGL IHATPFVIAA GGIMDSKDIS YMLSQQADAV QVGTAFLGCS 300
    ESNASKNFSS PFTRETTTKM VNIISGKPAR TISTPFIEKV IANFQGEELP 350
    PYGYMYSAFK QVRKKYPELA NFILAGQGFQ NVQSGITTDK KIETMGARLK 400
    IDGK 404
    Length:404
    Mass (Da):45,159
    Last modified:September 21, 2011 - v2
    Checksum:iE3FEE0E6C76ED7FA
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti402 – 4021D → V in CAA89682. (PubMed:8641269)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49649 Genomic DNA. Translation: CAA89682.1.
    BK006943 Genomic DNA. Translation: DAA08933.2.
    PIRiS57178.
    RefSeqiNP_012683.4. NM_001181807.4.

    Genome annotation databases

    EnsemblFungiiYJR149W; YJR149W; YJR149W.
    GeneIDi853614.
    KEGGisce:YJR149W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49649 Genomic DNA. Translation: CAA89682.1 .
    BK006943 Genomic DNA. Translation: DAA08933.2 .
    PIRi S57178.
    RefSeqi NP_012683.4. NM_001181807.4.

    3D structure databases

    ProteinModelPortali P47177.
    SMRi P47177. Positions 169-399.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33904. 8 interactions.
    IntActi P47177. 4 interactions.
    MINTi MINT-4083947.
    STRINGi 4932.YJR149W.

    Proteomic databases

    PaxDbi P47177.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR149W ; YJR149W ; YJR149W .
    GeneIDi 853614.
    KEGGi sce:YJR149W.

    Organism-specific databases

    CYGDi YJR149w.
    SGDi S000003910. YJR149W.

    Phylogenomic databases

    eggNOGi COG2070.
    HOGENOMi HOG000123287.
    KOi K00459.
    OMAi TQVGTMA.
    OrthoDBi EOG74N5SH.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31762-MONOMER.

    Miscellaneous databases

    NextBioi 974463.

    Gene expression databases

    Genevestigatori P47177.

    Family and domain databases

    Gene3Di 3.20.20.70. 2 hits.
    InterProi IPR004136. 2Npropane_dOase.
    IPR013785. Aldolase_TIM.
    [Graphical view ]
    Pfami PF03060. NMO. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 402.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry namei2NDP_YEAST
    AccessioniPrimary (citable) accession number: P47177
    Secondary accession number(s): D6VWW7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: September 21, 2011
    Last modified: October 1, 2014
    This is version 103 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 172 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3