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P47177 (2NDP_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Putative nitronate monooxygenase
EC=1.13.12.16
Alternative name(s):
Nitroalkane oxidase
Gene names
Ordered Locus Names:YJR149W
ORF Names:J2213
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length404 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites By similarity.

Catalytic activity

Ethylnitronate + O2 = acetaldehyde + nitrite + other products.

Cofactor

Binds 1 FMN per subunit By similarity.

Subcellular location

Cytoplasm Ref.3.

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the nitronate monooxygenase family.

Ontologies

Keywords
   Cellular componentCytoplasm
   LigandFMN
Flavoprotein
   Molecular functionMonooxygenase
Oxidoreductase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from direct assay Ref.3. Source: SGD

   Molecular_functionnitronate monooxygenase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

PRP40P332031EBI-25740,EBI-701
RSP5P399401EBI-25740,EBI-16219

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 404404Putative nitronate monooxygenase
PRO_0000203128

Regions

Nucleotide binding41 – 433FMN By similarity
Nucleotide binding270 – 2723FMN By similarity
Nucleotide binding293 – 2942FMN By similarity

Sites

Active site2241Proton acceptor Potential
Binding site2241Substrate By similarity

Experimental info

Sequence conflict4021D → V in CAA89682. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P47177 [UniParc].

Last modified September 21, 2011. Version 2.
Checksum: E3FEE0E6C76ED7FA

FASTA40445,159
        10         20         30         40         50         60 
MYFLNQLIFQ DVSVMSVDKR EDMSRSFQKC LNLRYPIIQA PMAGVTTIEM AAKACIAGAI 

        70         80         90        100        110        120 
ASLPLSHLDF RKVNDIEKLK LMVSQFRDQV ADESLEGNLN LNFFCHDIVD KPTDLQTANW 

       130        140        150        160        170        180 
AKLYRKSMNV PIDMNEIKFD NGNVSFKAFE KENALQDFFQ YLSDGFRPKI ISFHFGHPSK 

       190        200        210        220        230        240 
STIEYLQKIG ILIFVTATSV REVRLLARLG INGIVCQGYE AGGHRGNFLV NDPKDDENLS 

       250        260        270        280        290        300 
TVQLVKRTVD ELAEMKNKGL IHATPFVIAA GGIMDSKDIS YMLSQQADAV QVGTAFLGCS 

       310        320        330        340        350        360 
ESNASKNFSS PFTRETTTKM VNIISGKPAR TISTPFIEKV IANFQGEELP PYGYMYSAFK 

       370        380        390        400 
QVRKKYPELA NFILAGQGFQ NVQSGITTDK KIETMGARLK IDGK

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (MAR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 402.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z49649 Genomic DNA. Translation: CAA89682.1.
BK006943 Genomic DNA. Translation: DAA08933.2.
PIRS57178.
RefSeqNP_012683.4. NM_001181807.4.

3D structure databases

ProteinModelPortalP47177.
SMRP47177. Positions 169-399.
ModBaseSearch...

Protein-protein interaction databases

IntActP47177. 3 interactions.
MINTMINT-4083947.
STRING4932.YJR149W.

Proteomic databases

PaxDbP47177.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR149W; YJR149W; YJR149W.
GeneID853614.
KEGGsce:YJR149W.
sce:YJR152W.

Organism-specific databases

CYGDYJR149w.
SGDS000003910. YJR149W.

Phylogenomic databases

eggNOGCOG2070.
HOGENOMHOG000123287.
KOK00459.
K08192.
OMAPLNLNFF.
OrthoDBEOG4ZGSNJ.

Gene expression databases

GenevestigatorP47177.
GermOnlineYJR149W. Saccharomyces cerevisiae.

Family and domain databases

Gene3D3.20.20.70. 2 hits.
InterProIPR004136. 2Npropane_dOase.
IPR013785. Aldolase_TIM.
[Graphical view]
PfamPF03060. NMO. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974463.

Entry information

Entry name2NDP_YEAST
AccessionPrimary (citable) accession number: P47177
Secondary accession number(s): D6VWW7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 21, 2011
Last modified: May 1, 2013
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

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