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P47177

- 2NDP_YEAST

UniProt

P47177 - 2NDP_YEAST

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Protein
Putative nitronate monooxygenase
Gene
YJR149W, J2213
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites By similarity.

Catalytic activityi

Ethylnitronate + O2 = acetaldehyde + nitrite + other products.

Cofactori

Binds 1 FMN per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei224 – 2241Proton acceptor Reviewed prediction
Binding sitei224 – 2241Substrate By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi41 – 433FMN By similarity
Nucleotide bindingi270 – 2723FMN By similarity
Nucleotide bindingi293 – 2942FMN By similarity

GO - Molecular functioni

  1. nitronate monooxygenase activity Source: UniProtKB-EC
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Ligandi

Flavoprotein, FMN

Enzyme and pathway databases

BioCyciYEAST:G3O-31762-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative nitronate monooxygenase (EC:1.13.12.16)
Alternative name(s):
Nitroalkane oxidase
Gene namesi
Ordered Locus Names:YJR149W
ORF Names:J2213
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR149w.
SGDiS000003910. YJR149W.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 404404Putative nitronate monooxygenase
PRO_0000203128Add
BLAST

Proteomic databases

PaxDbiP47177.

Expressioni

Gene expression databases

GenevestigatoriP47177.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PRP40P332031EBI-25740,EBI-701
RSP5P399401EBI-25740,EBI-16219

Protein-protein interaction databases

BioGridi33904. 8 interactions.
IntActiP47177. 4 interactions.
MINTiMINT-4083947.
STRINGi4932.YJR149W.

Structurei

3D structure databases

ProteinModelPortaliP47177.
SMRiP47177. Positions 169-399.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG2070.
HOGENOMiHOG000123287.
KOiK00459.
OMAiTQVGTMA.
OrthoDBiEOG74N5SH.

Family and domain databases

Gene3Di3.20.20.70. 2 hits.
InterProiIPR004136. 2Npropane_dOase.
IPR013785. Aldolase_TIM.
[Graphical view]
PfamiPF03060. NMO. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47177-1 [UniParc]FASTAAdd to Basket

« Hide

MYFLNQLIFQ DVSVMSVDKR EDMSRSFQKC LNLRYPIIQA PMAGVTTIEM    50
AAKACIAGAI ASLPLSHLDF RKVNDIEKLK LMVSQFRDQV ADESLEGNLN 100
LNFFCHDIVD KPTDLQTANW AKLYRKSMNV PIDMNEIKFD NGNVSFKAFE 150
KENALQDFFQ YLSDGFRPKI ISFHFGHPSK STIEYLQKIG ILIFVTATSV 200
REVRLLARLG INGIVCQGYE AGGHRGNFLV NDPKDDENLS TVQLVKRTVD 250
ELAEMKNKGL IHATPFVIAA GGIMDSKDIS YMLSQQADAV QVGTAFLGCS 300
ESNASKNFSS PFTRETTTKM VNIISGKPAR TISTPFIEKV IANFQGEELP 350
PYGYMYSAFK QVRKKYPELA NFILAGQGFQ NVQSGITTDK KIETMGARLK 400
IDGK 404
Length:404
Mass (Da):45,159
Last modified:September 21, 2011 - v2
Checksum:iE3FEE0E6C76ED7FA
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti402 – 4021D → V in CAA89682. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49649 Genomic DNA. Translation: CAA89682.1.
BK006943 Genomic DNA. Translation: DAA08933.2.
PIRiS57178.
RefSeqiNP_012683.4. NM_001181807.4.

Genome annotation databases

EnsemblFungiiYJR149W; YJR149W; YJR149W.
GeneIDi853614.
KEGGisce:YJR149W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49649 Genomic DNA. Translation: CAA89682.1 .
BK006943 Genomic DNA. Translation: DAA08933.2 .
PIRi S57178.
RefSeqi NP_012683.4. NM_001181807.4.

3D structure databases

ProteinModelPortali P47177.
SMRi P47177. Positions 169-399.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33904. 8 interactions.
IntActi P47177. 4 interactions.
MINTi MINT-4083947.
STRINGi 4932.YJR149W.

Proteomic databases

PaxDbi P47177.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR149W ; YJR149W ; YJR149W .
GeneIDi 853614.
KEGGi sce:YJR149W.

Organism-specific databases

CYGDi YJR149w.
SGDi S000003910. YJR149W.

Phylogenomic databases

eggNOGi COG2070.
HOGENOMi HOG000123287.
KOi K00459.
OMAi TQVGTMA.
OrthoDBi EOG74N5SH.

Enzyme and pathway databases

BioCyci YEAST:G3O-31762-MONOMER.

Miscellaneous databases

NextBioi 974463.

Gene expression databases

Genevestigatori P47177.

Family and domain databases

Gene3Di 3.20.20.70. 2 hits.
InterProi IPR004136. 2Npropane_dOase.
IPR013785. Aldolase_TIM.
[Graphical view ]
Pfami PF03060. NMO. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 402.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry namei2NDP_YEAST
AccessioniPrimary (citable) accession number: P47177
Secondary accession number(s): D6VWW7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 21, 2011
Last modified: June 11, 2014
This is version 102 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

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