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Protein

Putative nitronate monooxygenase

Gene

YJR149W

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the oxidation of alkyl nitronates to produce the corresponding carbonyl compounds and nitrites.By similarity

Miscellaneous

Present with 172 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

Ethylnitronate + O2 = acetaldehyde + nitrite + other products.

Cofactori

FMNBy similarityNote: Binds 1 FMN per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei224Proton acceptorSequence analysis1
Binding sitei224SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi41 – 43FMNBy similarity3
Nucleotide bindingi270 – 272FMNBy similarity3
Nucleotide bindingi293 – 294FMNBy similarity2

GO - Molecular functioni

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
LigandFlavoprotein, FMN

Enzyme and pathway databases

BioCyciYEAST:G3O-31762-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Putative nitronate monooxygenase (EC:1.13.12.16)
Alternative name(s):
Nitroalkane oxidase
Gene namesi
Ordered Locus Names:YJR149W
ORF Names:J2213
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR149W
SGDiS000003910 YJR149W

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002031281 – 404Putative nitronate monooxygenaseAdd BLAST404

Proteomic databases

PaxDbiP47177
PRIDEiP47177

Interactioni

Protein-protein interaction databases

BioGridi33904, 68 interactors
IntActiP47177, 4 interactors
MINTiP47177
STRINGi4932.YJR149W

Structurei

3D structure databases

ProteinModelPortaliP47177
SMRiP47177
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the nitronate monooxygenase family.Curated

Phylogenomic databases

HOGENOMiHOG000123287
InParanoidiP47177
KOiK00459
OMAiQGMALWA
OrthoDBiEOG092C3IJS

Family and domain databases

Gene3Di3.20.20.70, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR004136 NMO
PfamiView protein in Pfam
PF03060 NMO, 1 hit

Sequencei

Sequence statusi: Complete.

P47177-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MYFLNQLIFQ DVSVMSVDKR EDMSRSFQKC LNLRYPIIQA PMAGVTTIEM
60 70 80 90 100
AAKACIAGAI ASLPLSHLDF RKVNDIEKLK LMVSQFRDQV ADESLEGNLN
110 120 130 140 150
LNFFCHDIVD KPTDLQTANW AKLYRKSMNV PIDMNEIKFD NGNVSFKAFE
160 170 180 190 200
KENALQDFFQ YLSDGFRPKI ISFHFGHPSK STIEYLQKIG ILIFVTATSV
210 220 230 240 250
REVRLLARLG INGIVCQGYE AGGHRGNFLV NDPKDDENLS TVQLVKRTVD
260 270 280 290 300
ELAEMKNKGL IHATPFVIAA GGIMDSKDIS YMLSQQADAV QVGTAFLGCS
310 320 330 340 350
ESNASKNFSS PFTRETTTKM VNIISGKPAR TISTPFIEKV IANFQGEELP
360 370 380 390 400
PYGYMYSAFK QVRKKYPELA NFILAGQGFQ NVQSGITTDK KIETMGARLK

IDGK
Length:404
Mass (Da):45,159
Last modified:September 21, 2011 - v2
Checksum:iE3FEE0E6C76ED7FA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti402D → V in CAA89682 (PubMed:8641269).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49649 Genomic DNA Translation: CAA89682.1
BK006943 Genomic DNA Translation: DAA08933.2
PIRiS57178
RefSeqiNP_012683.4, NM_001181807.4

Genome annotation databases

EnsemblFungiiYJR149W; YJR149W; YJR149W
GeneIDi853614
KEGGisce:YJR149W

Similar proteinsi

Entry informationi

Entry namei2NDP_YEAST
AccessioniPrimary (citable) accession number: P47177
Secondary accession number(s): D6VWW7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 21, 2011
Last modified: April 25, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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