P47170 (IML1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar membrane-associated protein IML1

Alternative name(s):
Increased minichromosome loss protein 1
SEH-associated protein 1

Gene names

Name:	IML1
Synonyms:	SEA1
Ordered Locus Names:	YJR138W
ORF Names:	J2129

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	1584 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. Ref.8
Subunit structure	Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1. Ref.8
Subcellular location	Vacuole membrane; Peripheral membrane protein Ref.3 Ref.8.
Miscellaneous	Present with 279 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the IML1 family. Contains 1 DEP domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Vacuole
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Seh1-associated complex Inferred from direct assay Ref.8. Source: SGD extrinsic to fungal-type vacuolar membrane Inferred from direct assay Ref.8. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VAC8	P39968	1	EBI-25710,EBI-20212

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1584	1584	Vacuolar membrane-associated protein IML1		PRO_0000203123
Regions
	Domain	1198 – 1273	76	DEP
Amino acid modifications
	Modified residue	680	1	Phosphoserine Ref.5 Ref.7
	Modified residue	737	1	Phosphoserine Ref.7
	Modified residue	835	1	Phosphoserine Ref.7
	Modified residue	1291	1	Phosphothreonine Ref.6
	Modified residue	1293	1	Phosphoserine Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P47170 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: BB8E73FF5B70CCDF	FASTA	1,584	181,949
	10 20 30 40 50 60 MFAKLHGKKQ RPISSINSQT PRTSNTTHAN SISLSSGNLI VGSNRNLRQK KEQFGSQQRA 70 80 90 100 110 120 SGRKLISNKE NDDNVNNGGD NNYDNGERVH RHHIPGLKIK AYQAELGYHE SRFSENLVML 130 140 150 160 170 180 NLVEFPDIKP GDLVELKTYH KNPSASNGDK KIYFIAKDFD GETKRRAKTS NVSILSGQLQ 190 200 210 220 230 240 TLLDLPSRSR IWIKLKPNKF DLQADVVEFN IKDCLLNRGD MWVLSSKLVD TCVFMDQRLA 250 260 270 280 290 300 FLDSIRGTIK GIYRNGKKIV SGYIGEQTRI IFRSESARLI FLIQITDEMW NFEETGEQLF 310 320 330 340 350 360 QKMVNSFFPK IFKKWKDVDT HHTITIAFAI SMDLSDTSFK DLTPGESLKN SQDYFRIVVD 370 380 390 400 410 420 QVSIIHWVDI METLREEFME IRKDLLNKQT DKGYSVANGR FSPVIKSNFL ELVNFATTIL 430 440 450 460 470 480 TDPFKQLDLR HTTTHVMIIS PGSGLFDVDY SLLRLTGKKL LSLEMTMDLI CLSKAPLHIV 490 500 510 520 530 540 PLFRYRDFEN KLHHCVPLWL SVFFWNDHDK KSNSEWTPRC KIYDLQMMGI TENELIREVD 550 560 570 580 590 600 VEYLQLNKKV KSLSEFMNDY DKNAFEVKIL CAGSNTKQSK KLNSKFDTVF ENDVVVKARK 610 620 630 640 650 660 IPATATTTHG NTKFIWRGPK VALPAIKDIQ KPNVIPDLSI KTIEASFYDD CNTTNDKIST 670 680 690 700 710 720 PTTSNNDNLE MNDSLVSVRS ADNQNTSLAL DSLKGLSKRN SLKDFTQRVI TKFISNIDTS 730 740 750 760 770 780 KNKKIKSTLL RDDVDNSPLG SNTPLPSSES KISGLKLQQK GLADENVISK RGNLIIKKNL 790 800 810 820 830 840 SIFGLPSNEI MSGSPSSYLG SSHTRTSSKL SNMSDKAAFI TEGQKSKHDD SNTYSLTQQL 850 860 870 880 890 900 KHRISETWVD IKSPSIPVSS EFANELLPIR WKDVWPKYVA RKYSKWRSFT TPAELPITIS 910 920 930 940 950 960 DFPSKDDFDR NFIFRNHSVT LNTDQEQYNQ TYKDLLRDMI YMRLLTGFQI CVGRQVEKIE 970 980 990 1000 1010 1020 LSRESGESET VVNKYLDFNQ NDAFKLYLMI DSEIHRITCS SSGIIDVERY LRKDEANLFD 1030 1040 1050 1060 1070 1080 QVPSYIPLVK TRYESSFRDA MIDPLHVKRE SLNWNQIDQV LAGYGDNLID RKWHGFRAKY 1090 1100 1110 1120 1130 1140 VVLPTDIPPN TYSMVINGKS ETLNPEEIRV EGLRRLIGSI TRSRLRTEKE KKGRKTKREE 1150 1160 1170 1180 1190 1200 IQPEVMFYTG PLYNFINEQQ TSLESSAINF KDSIFVNDNN LLNRNVELSK LAYQIQRGED 1210 1220 1230 1240 1250 1260 RITLVNRKWH WKKHEKCFVG SEMVNWLIRN FSDIDTREDA IKYGQKVMKE GLFVHVLNKH 1270 1280 1290 1300 1310 1320 NFLDGHYFYQ FSPEYVMDTN KLEKTNSHRS TLSDPKQMLR KASTGSSNDP SAMTPFSSVV 1330 1340 1350 1360 1370 1380 PAISASNASV ADAKEPSRPI LMLSNSLVID VDPAGKSSKQ ESCTVHYDRV HNPDHCFHIR 1390 1400 1410 1420 1430 1440 LEWLTTTPKL IDDLVGNWSR LCERYGLKMI EIPWEELCTI PSVNPFHSFV EIKLAINPWE 1450 1460 1470 1480 1490 1500 DPEFKDRELF AKSKFYYHVY LLKASGFLLD NRASKFLQNQ DIEFDIMYSW GKPQFKYVQY 1510 1520 1530 1540 1550 1560 IHHTGAYVAE LRENGCLFLA PNNIYISRVN PGNIIGKIHS ASSSSLDAQK VILNFKSTCL 1570 1580 DYQKLRSIFL DAKEMWITGK IVED

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, MASS SPECTROMETRY. Strain: ADR376.
[6]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1291, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680; SER-737; SER-835 AND SER-1293, MASS SPECTROMETRY.
[8]	"A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae." Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C. Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49638 Genomic DNA. Translation: CAA89670.1. BK006943 Genomic DNA. Translation: DAA08923.1.
PIR	S57161.
RefSeq	NP_012672.1. NM_001181796.2.
3D structure databases
ProteinModelPortal	P47170.
SMR	P47170. Positions 1206-1271.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2589N.
IntAct	P47170. 15 interactions.
MINT	MINT-423965.
STRING	4932.YJR138W.
Proteomic databases
PaxDb	P47170.
PeptideAtlas	P47170.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR138W; YJR138W; YJR138W.
GeneID	853603.
KEGG	sce:YJR138W.
Organism-specific databases
CYGD	YJR138w.
SGD	S000003899. IML1.
Phylogenomic databases
eggNOG	NOG235380.
GeneTree	ENSGT00390000016559.
HOGENOM	HOG000093241.
OMA	ELQMMGV.
OrthoDB	EOG4S7NZN.
Gene expression databases
Genevestigator	P47170.
GermOnline	YJR138W. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000591. DEP_dom. IPR027244. IML1. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13179:SF8. PTHR13179:SF8. 1 hit.
Pfam	PF00610. DEP. 1 hit. PF12257. DUF3608. 1 hit. [Graphical view]
SMART	SM00049. DEP. 1 hit. [Graphical view]
PROSITE	PS50186. DEP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974433.

Entry information

Entry name

IML1_YEAST

Accession

Primary (citable) accession number: P47170
Secondary accession number(s): D6VWV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 99 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program