Vacuolar membrane-associated protein IML1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47170 (IML1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 103. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Vacuolar membrane-associated protein IML1

Alternative name(s):
Increased minichromosome loss protein 1
SEH-associated protein 1

Gene names

Name:	IML1
Synonyms:	SEA1
Ordered Locus Names:	YJR138W
ORF Names:	J2129

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1584 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. Ref.8
Subunit structure	Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1. Ref.8
Subcellular location	Vacuole membrane; Peripheral membrane protein Ref.3 Ref.8.
Miscellaneous	Present with 279 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the IML1 family. Contains 1 DEP domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Membrane Vacuole
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	intracellular signal transduction Inferred from electronic annotation. Source: InterPro protein transport Inferred from electronic annotation. Source: UniProtKB-KW regulation of autophagic vacuole assembly Inferred from mutant phenotype PubMed 21900499. Source: SGD
Cellular_component	Iml1 complex Inferred from direct assay PubMed 21900499. Source: SGD Seh1-associated complex Inferred from direct assay Ref.8. Source: SGD extrinsic to fungal-type vacuolar membrane Inferred from direct assay Ref.8. Source: SGD
Molecular_function	GTPase activator activity Inferred from direct assay PubMed 23716719. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
VAC8	P39968	1	EBI-25710,EBI-20212

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1584

1584

Vacuolar membrane-associated protein IML1

PRO_0000203123

Regions

Domain

1198 – 1273

DEP

Amino acid modifications

Modified residue

680

Phosphoserine Ref.5 Ref.6 Ref.7

Modified residue

737

Phosphoserine Ref.6

Sequences

Sequence

Length

Mass (Da)

Tools

P47170 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: BB8E73FF5B70CCDF
Show »

FASTA

1,584

181,949

        10         20         30         40         50         60 
MFAKLHGKKQ RPISSINSQT PRTSNTTHAN SISLSSGNLI VGSNRNLRQK KEQFGSQQRA 

        70         80         90        100        110        120 
SGRKLISNKE NDDNVNNGGD NNYDNGERVH RHHIPGLKIK AYQAELGYHE SRFSENLVML 

       130        140        150        160        170        180 
NLVEFPDIKP GDLVELKTYH KNPSASNGDK KIYFIAKDFD GETKRRAKTS NVSILSGQLQ 

       190        200        210        220        230        240 
TLLDLPSRSR IWIKLKPNKF DLQADVVEFN IKDCLLNRGD MWVLSSKLVD TCVFMDQRLA 

       250        260        270        280        290        300 
FLDSIRGTIK GIYRNGKKIV SGYIGEQTRI IFRSESARLI FLIQITDEMW NFEETGEQLF 

       310        320        330        340        350        360 
QKMVNSFFPK IFKKWKDVDT HHTITIAFAI SMDLSDTSFK DLTPGESLKN SQDYFRIVVD 

       370        380        390        400        410        420 
QVSIIHWVDI METLREEFME IRKDLLNKQT DKGYSVANGR FSPVIKSNFL ELVNFATTIL 

       430        440        450        460        470        480 
TDPFKQLDLR HTTTHVMIIS PGSGLFDVDY SLLRLTGKKL LSLEMTMDLI CLSKAPLHIV 

       490        500        510        520        530        540 
PLFRYRDFEN KLHHCVPLWL SVFFWNDHDK KSNSEWTPRC KIYDLQMMGI TENELIREVD 

       550        560        570        580        590        600 
VEYLQLNKKV KSLSEFMNDY DKNAFEVKIL CAGSNTKQSK KLNSKFDTVF ENDVVVKARK 

       610        620        630        640        650        660 
IPATATTTHG NTKFIWRGPK VALPAIKDIQ KPNVIPDLSI KTIEASFYDD CNTTNDKIST 

       670        680        690        700        710        720 
PTTSNNDNLE MNDSLVSVRS ADNQNTSLAL DSLKGLSKRN SLKDFTQRVI TKFISNIDTS 

       730        740        750        760        770        780 
KNKKIKSTLL RDDVDNSPLG SNTPLPSSES KISGLKLQQK GLADENVISK RGNLIIKKNL 

       790        800        810        820        830        840 
SIFGLPSNEI MSGSPSSYLG SSHTRTSSKL SNMSDKAAFI TEGQKSKHDD SNTYSLTQQL 

       850        860        870        880        890        900 
KHRISETWVD IKSPSIPVSS EFANELLPIR WKDVWPKYVA RKYSKWRSFT TPAELPITIS 

       910        920        930        940        950        960 
DFPSKDDFDR NFIFRNHSVT LNTDQEQYNQ TYKDLLRDMI YMRLLTGFQI CVGRQVEKIE 

       970        980        990       1000       1010       1020 
LSRESGESET VVNKYLDFNQ NDAFKLYLMI DSEIHRITCS SSGIIDVERY LRKDEANLFD 

      1030       1040       1050       1060       1070       1080 
QVPSYIPLVK TRYESSFRDA MIDPLHVKRE SLNWNQIDQV LAGYGDNLID RKWHGFRAKY 

      1090       1100       1110       1120       1130       1140 
VVLPTDIPPN TYSMVINGKS ETLNPEEIRV EGLRRLIGSI TRSRLRTEKE KKGRKTKREE 

      1150       1160       1170       1180       1190       1200 
IQPEVMFYTG PLYNFINEQQ TSLESSAINF KDSIFVNDNN LLNRNVELSK LAYQIQRGED 

      1210       1220       1230       1240       1250       1260 
RITLVNRKWH WKKHEKCFVG SEMVNWLIRN FSDIDTREDA IKYGQKVMKE GLFVHVLNKH 

      1270       1280       1290       1300       1310       1320 
NFLDGHYFYQ FSPEYVMDTN KLEKTNSHRS TLSDPKQMLR KASTGSSNDP SAMTPFSSVV 

      1330       1340       1350       1360       1370       1380 
PAISASNASV ADAKEPSRPI LMLSNSLVID VDPAGKSSKQ ESCTVHYDRV HNPDHCFHIR 

      1390       1400       1410       1420       1430       1440 
LEWLTTTPKL IDDLVGNWSR LCERYGLKMI EIPWEELCTI PSVNPFHSFV EIKLAINPWE 

      1450       1460       1470       1480       1490       1500 
DPEFKDRELF AKSKFYYHVY LLKASGFLLD NRASKFLQNQ DIEFDIMYSW GKPQFKYVQY 

      1510       1520       1530       1540       1550       1560 
IHHTGAYVAE LRENGCLFLA PNNIYISRVN PGNIIGKIHS ASSSSLDAQK VILNFKSTCL 

      1570       1580 
DYQKLRSIFL DAKEMWITGK IVED

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, MASS SPECTROMETRY. Strain: ADR376.
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680 AND SER-737, MASS SPECTROMETRY.
[7]	"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution." Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O. Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, MASS SPECTROMETRY.
[8]	"A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae." Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C. Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49638 Genomic DNA. Translation: CAA89670.1. BK006943 Genomic DNA. Translation: DAA08923.1.
PIR	S57161.
RefSeq	NP_012672.1. NM_001181796.2.
3D structure databases
ProteinModelPortal	P47170.
SMR	P47170. Positions 1206-1271.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2589N.
IntAct	P47170. 21 interactions.
MINT	MINT-423965.
STRING	4932.YJR138W.
Proteomic databases
PaxDb	P47170.
PeptideAtlas	P47170.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR138W; YJR138W; YJR138W.
GeneID	853603.
KEGG	sce:YJR138W.
Organism-specific databases
CYGD	YJR138w.
SGD	S000003899. IML1.
Phylogenomic databases
eggNOG	NOG235380.
GeneTree	ENSGT00390000016559.
HOGENOM	HOG000093241.
OMA	SMSNEIH.
OrthoDB	EOG7M3J7T.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31754-MONOMER.
Gene expression databases
Genevestigator	P47170.
Family and domain databases
Gene3D	1.10.10.10. 1 hit.
InterPro	IPR000591. DEP_dom. IPR027244. IML1. IPR011991. WHTH_DNA-bd_dom. [Graphical view]
PANTHER	PTHR13179:SF8. PTHR13179:SF8. 1 hit.
Pfam	PF00610. DEP. 1 hit. PF12257. DUF3608. 1 hit. [Graphical view]
SMART	SM00049. DEP. 1 hit. [Graphical view]
PROSITE	PS50186. DEP. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974433.
PRO	P47170.

Entry information

Entry name

IML1_YEAST

Accession

Primary (citable) accession number: P47170
Secondary accession number(s): D6VWV7

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 13, 2013
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Binary interactions

With

Entry

#Exp.

IntAct

Notes

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents