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P47170

- IML1_YEAST

UniProt

P47170 - IML1_YEAST

Protein

Vacuolar membrane-associated protein IML1

Gene

IML1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 110 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.1 Publication

    GO - Molecular functioni

    1. GTPase activator activity Source: SGD

    GO - Biological processi

    1. intracellular signal transduction Source: InterPro
    2. positive regulation of GTPase activity Source: GOC
    3. protein transport Source: UniProtKB-KW
    4. regulation of autophagic vacuole assembly Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31754-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar membrane-associated protein IML1
    Alternative name(s):
    Increased minichromosome loss protein 1
    SEH-associated protein 1
    Gene namesi
    Name:IML1
    Synonyms:SEA1
    Ordered Locus Names:YJR138W
    ORF Names:J2129
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR138w.
    SGDiS000003899. IML1.

    Subcellular locationi

    Vacuole membrane 2 Publications; Peripheral membrane protein 2 Publications

    GO - Cellular componenti

    1. extrinsic component of fungal-type vacuolar membrane Source: SGD
    2. Iml1 complex Source: SGD
    3. Seh1-associated complex Source: SGD

    Keywords - Cellular componenti

    Membrane, Vacuole

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 15841584Vacuolar membrane-associated protein IML1PRO_0000203123Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei680 – 6801Phosphoserine3 Publications
    Modified residuei737 – 7371Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47170.
    PaxDbiP47170.
    PeptideAtlasiP47170.

    Expressioni

    Gene expression databases

    GenevestigatoriP47170.

    Interactioni

    Subunit structurei

    Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    VAC8P399681EBI-25710,EBI-20212

    Protein-protein interaction databases

    BioGridi33894. 34 interactions.
    DIPiDIP-2589N.
    IntActiP47170. 21 interactions.
    MINTiMINT-423965.
    STRINGi4932.YJR138W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47170.
    SMRiP47170. Positions 1206-1271.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini1198 – 127376DEPPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the IML1 family.Curated
    Contains 1 DEP domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiNOG235380.
    GeneTreeiENSGT00390000016559.
    HOGENOMiHOG000093241.
    OMAiSMSNEIH.
    OrthoDBiEOG7M3J7T.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    InterProiIPR000591. DEP_dom.
    IPR027244. IML1.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR13179. PTHR13179. 1 hit.
    PfamiPF00610. DEP. 1 hit.
    PF12257. DUF3608. 1 hit.
    [Graphical view]
    SMARTiSM00049. DEP. 1 hit.
    [Graphical view]
    PROSITEiPS50186. DEP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47170-1 [UniParc]FASTAAdd to Basket

    « Hide

    MFAKLHGKKQ RPISSINSQT PRTSNTTHAN SISLSSGNLI VGSNRNLRQK     50
    KEQFGSQQRA SGRKLISNKE NDDNVNNGGD NNYDNGERVH RHHIPGLKIK 100
    AYQAELGYHE SRFSENLVML NLVEFPDIKP GDLVELKTYH KNPSASNGDK 150
    KIYFIAKDFD GETKRRAKTS NVSILSGQLQ TLLDLPSRSR IWIKLKPNKF 200
    DLQADVVEFN IKDCLLNRGD MWVLSSKLVD TCVFMDQRLA FLDSIRGTIK 250
    GIYRNGKKIV SGYIGEQTRI IFRSESARLI FLIQITDEMW NFEETGEQLF 300
    QKMVNSFFPK IFKKWKDVDT HHTITIAFAI SMDLSDTSFK DLTPGESLKN 350
    SQDYFRIVVD QVSIIHWVDI METLREEFME IRKDLLNKQT DKGYSVANGR 400
    FSPVIKSNFL ELVNFATTIL TDPFKQLDLR HTTTHVMIIS PGSGLFDVDY 450
    SLLRLTGKKL LSLEMTMDLI CLSKAPLHIV PLFRYRDFEN KLHHCVPLWL 500
    SVFFWNDHDK KSNSEWTPRC KIYDLQMMGI TENELIREVD VEYLQLNKKV 550
    KSLSEFMNDY DKNAFEVKIL CAGSNTKQSK KLNSKFDTVF ENDVVVKARK 600
    IPATATTTHG NTKFIWRGPK VALPAIKDIQ KPNVIPDLSI KTIEASFYDD 650
    CNTTNDKIST PTTSNNDNLE MNDSLVSVRS ADNQNTSLAL DSLKGLSKRN 700
    SLKDFTQRVI TKFISNIDTS KNKKIKSTLL RDDVDNSPLG SNTPLPSSES 750
    KISGLKLQQK GLADENVISK RGNLIIKKNL SIFGLPSNEI MSGSPSSYLG 800
    SSHTRTSSKL SNMSDKAAFI TEGQKSKHDD SNTYSLTQQL KHRISETWVD 850
    IKSPSIPVSS EFANELLPIR WKDVWPKYVA RKYSKWRSFT TPAELPITIS 900
    DFPSKDDFDR NFIFRNHSVT LNTDQEQYNQ TYKDLLRDMI YMRLLTGFQI 950
    CVGRQVEKIE LSRESGESET VVNKYLDFNQ NDAFKLYLMI DSEIHRITCS 1000
    SSGIIDVERY LRKDEANLFD QVPSYIPLVK TRYESSFRDA MIDPLHVKRE 1050
    SLNWNQIDQV LAGYGDNLID RKWHGFRAKY VVLPTDIPPN TYSMVINGKS 1100
    ETLNPEEIRV EGLRRLIGSI TRSRLRTEKE KKGRKTKREE IQPEVMFYTG 1150
    PLYNFINEQQ TSLESSAINF KDSIFVNDNN LLNRNVELSK LAYQIQRGED 1200
    RITLVNRKWH WKKHEKCFVG SEMVNWLIRN FSDIDTREDA IKYGQKVMKE 1250
    GLFVHVLNKH NFLDGHYFYQ FSPEYVMDTN KLEKTNSHRS TLSDPKQMLR 1300
    KASTGSSNDP SAMTPFSSVV PAISASNASV ADAKEPSRPI LMLSNSLVID 1350
    VDPAGKSSKQ ESCTVHYDRV HNPDHCFHIR LEWLTTTPKL IDDLVGNWSR 1400
    LCERYGLKMI EIPWEELCTI PSVNPFHSFV EIKLAINPWE DPEFKDRELF 1450
    AKSKFYYHVY LLKASGFLLD NRASKFLQNQ DIEFDIMYSW GKPQFKYVQY 1500
    IHHTGAYVAE LRENGCLFLA PNNIYISRVN PGNIIGKIHS ASSSSLDAQK 1550
    VILNFKSTCL DYQKLRSIFL DAKEMWITGK IVED 1584
    Length:1,584
    Mass (Da):181,949
    Last modified:February 1, 1996 - v1
    Checksum:iBB8E73FF5B70CCDF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49638 Genomic DNA. Translation: CAA89670.1.
    BK006943 Genomic DNA. Translation: DAA08923.1.
    PIRiS57161.
    RefSeqiNP_012672.1. NM_001181796.2.

    Genome annotation databases

    EnsemblFungiiYJR138W; YJR138W; YJR138W.
    GeneIDi853603.
    KEGGisce:YJR138W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49638 Genomic DNA. Translation: CAA89670.1 .
    BK006943 Genomic DNA. Translation: DAA08923.1 .
    PIRi S57161.
    RefSeqi NP_012672.1. NM_001181796.2.

    3D structure databases

    ProteinModelPortali P47170.
    SMRi P47170. Positions 1206-1271.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33894. 34 interactions.
    DIPi DIP-2589N.
    IntActi P47170. 21 interactions.
    MINTi MINT-423965.
    STRINGi 4932.YJR138W.

    Proteomic databases

    MaxQBi P47170.
    PaxDbi P47170.
    PeptideAtlasi P47170.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR138W ; YJR138W ; YJR138W .
    GeneIDi 853603.
    KEGGi sce:YJR138W.

    Organism-specific databases

    CYGDi YJR138w.
    SGDi S000003899. IML1.

    Phylogenomic databases

    eggNOGi NOG235380.
    GeneTreei ENSGT00390000016559.
    HOGENOMi HOG000093241.
    OMAi SMSNEIH.
    OrthoDBi EOG7M3J7T.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31754-MONOMER.

    Miscellaneous databases

    NextBioi 974433.
    PROi P47170.

    Gene expression databases

    Genevestigatori P47170.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    InterProi IPR000591. DEP_dom.
    IPR027244. IML1.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR13179. PTHR13179. 1 hit.
    Pfami PF00610. DEP. 1 hit.
    PF12257. DUF3608. 1 hit.
    [Graphical view ]
    SMARTi SM00049. DEP. 1 hit.
    [Graphical view ]
    PROSITEi PS50186. DEP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680 AND SER-737, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae."
      Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C.
      Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.

    Entry informationi

    Entry nameiIML1_YEAST
    AccessioniPrimary (citable) accession number: P47170
    Secondary accession number(s): D6VWV7
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 110 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 279 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3