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P47170

- IML1_YEAST

UniProt

P47170 - IML1_YEAST

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Protein
Vacuolar membrane-associated protein IML1
Gene
IML1, SEA1, YJR138W, J2129
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis.1 Publication

GO - Molecular functioni

  1. GTPase activator activity Source: SGD

GO - Biological processi

  1. intracellular signal transduction Source: InterPro
  2. positive regulation of GTPase activity Source: GOC
  3. protein transport Source: UniProtKB-KW
  4. regulation of autophagic vacuole assembly Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31754-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar membrane-associated protein IML1
Alternative name(s):
Increased minichromosome loss protein 1
SEH-associated protein 1
Gene namesi
Name:IML1
Synonyms:SEA1
Ordered Locus Names:YJR138W
ORF Names:J2129
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR138w.
SGDiS000003899. IML1.

Subcellular locationi

Vacuole membrane; Peripheral membrane protein 2 Publications

GO - Cellular componenti

  1. Iml1 complex Source: SGD
  2. Seh1-associated complex Source: SGD
  3. extrinsic component of fungal-type vacuolar membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Vacuole

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 15841584Vacuolar membrane-associated protein IML1
PRO_0000203123Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei680 – 6801Phosphoserine3 Publications
Modified residuei737 – 7371Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47170.
PaxDbiP47170.
PeptideAtlasiP47170.

Expressioni

Gene expression databases

GenevestigatoriP47170.

Interactioni

Subunit structurei

Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
VAC8P399681EBI-25710,EBI-20212

Protein-protein interaction databases

BioGridi33894. 34 interactions.
DIPiDIP-2589N.
IntActiP47170. 21 interactions.
MINTiMINT-423965.
STRINGi4932.YJR138W.

Structurei

3D structure databases

ProteinModelPortaliP47170.
SMRiP47170. Positions 1206-1271.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1198 – 127376DEP
Add
BLAST

Sequence similaritiesi

Belongs to the IML1 family.
Contains 1 DEP domain.

Phylogenomic databases

eggNOGiNOG235380.
GeneTreeiENSGT00390000016559.
HOGENOMiHOG000093241.
OMAiSMSNEIH.
OrthoDBiEOG7M3J7T.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
InterProiIPR000591. DEP_dom.
IPR027244. IML1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13179. PTHR13179. 1 hit.
PfamiPF00610. DEP. 1 hit.
PF12257. DUF3608. 1 hit.
[Graphical view]
SMARTiSM00049. DEP. 1 hit.
[Graphical view]
PROSITEiPS50186. DEP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47170-1 [UniParc]FASTAAdd to Basket

« Hide

MFAKLHGKKQ RPISSINSQT PRTSNTTHAN SISLSSGNLI VGSNRNLRQK     50
KEQFGSQQRA SGRKLISNKE NDDNVNNGGD NNYDNGERVH RHHIPGLKIK 100
AYQAELGYHE SRFSENLVML NLVEFPDIKP GDLVELKTYH KNPSASNGDK 150
KIYFIAKDFD GETKRRAKTS NVSILSGQLQ TLLDLPSRSR IWIKLKPNKF 200
DLQADVVEFN IKDCLLNRGD MWVLSSKLVD TCVFMDQRLA FLDSIRGTIK 250
GIYRNGKKIV SGYIGEQTRI IFRSESARLI FLIQITDEMW NFEETGEQLF 300
QKMVNSFFPK IFKKWKDVDT HHTITIAFAI SMDLSDTSFK DLTPGESLKN 350
SQDYFRIVVD QVSIIHWVDI METLREEFME IRKDLLNKQT DKGYSVANGR 400
FSPVIKSNFL ELVNFATTIL TDPFKQLDLR HTTTHVMIIS PGSGLFDVDY 450
SLLRLTGKKL LSLEMTMDLI CLSKAPLHIV PLFRYRDFEN KLHHCVPLWL 500
SVFFWNDHDK KSNSEWTPRC KIYDLQMMGI TENELIREVD VEYLQLNKKV 550
KSLSEFMNDY DKNAFEVKIL CAGSNTKQSK KLNSKFDTVF ENDVVVKARK 600
IPATATTTHG NTKFIWRGPK VALPAIKDIQ KPNVIPDLSI KTIEASFYDD 650
CNTTNDKIST PTTSNNDNLE MNDSLVSVRS ADNQNTSLAL DSLKGLSKRN 700
SLKDFTQRVI TKFISNIDTS KNKKIKSTLL RDDVDNSPLG SNTPLPSSES 750
KISGLKLQQK GLADENVISK RGNLIIKKNL SIFGLPSNEI MSGSPSSYLG 800
SSHTRTSSKL SNMSDKAAFI TEGQKSKHDD SNTYSLTQQL KHRISETWVD 850
IKSPSIPVSS EFANELLPIR WKDVWPKYVA RKYSKWRSFT TPAELPITIS 900
DFPSKDDFDR NFIFRNHSVT LNTDQEQYNQ TYKDLLRDMI YMRLLTGFQI 950
CVGRQVEKIE LSRESGESET VVNKYLDFNQ NDAFKLYLMI DSEIHRITCS 1000
SSGIIDVERY LRKDEANLFD QVPSYIPLVK TRYESSFRDA MIDPLHVKRE 1050
SLNWNQIDQV LAGYGDNLID RKWHGFRAKY VVLPTDIPPN TYSMVINGKS 1100
ETLNPEEIRV EGLRRLIGSI TRSRLRTEKE KKGRKTKREE IQPEVMFYTG 1150
PLYNFINEQQ TSLESSAINF KDSIFVNDNN LLNRNVELSK LAYQIQRGED 1200
RITLVNRKWH WKKHEKCFVG SEMVNWLIRN FSDIDTREDA IKYGQKVMKE 1250
GLFVHVLNKH NFLDGHYFYQ FSPEYVMDTN KLEKTNSHRS TLSDPKQMLR 1300
KASTGSSNDP SAMTPFSSVV PAISASNASV ADAKEPSRPI LMLSNSLVID 1350
VDPAGKSSKQ ESCTVHYDRV HNPDHCFHIR LEWLTTTPKL IDDLVGNWSR 1400
LCERYGLKMI EIPWEELCTI PSVNPFHSFV EIKLAINPWE DPEFKDRELF 1450
AKSKFYYHVY LLKASGFLLD NRASKFLQNQ DIEFDIMYSW GKPQFKYVQY 1500
IHHTGAYVAE LRENGCLFLA PNNIYISRVN PGNIIGKIHS ASSSSLDAQK 1550
VILNFKSTCL DYQKLRSIFL DAKEMWITGK IVED 1584
Length:1,584
Mass (Da):181,949
Last modified:February 1, 1996 - v1
Checksum:iBB8E73FF5B70CCDF
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49638 Genomic DNA. Translation: CAA89670.1.
BK006943 Genomic DNA. Translation: DAA08923.1.
PIRiS57161.
RefSeqiNP_012672.1. NM_001181796.2.

Genome annotation databases

EnsemblFungiiYJR138W; YJR138W; YJR138W.
GeneIDi853603.
KEGGisce:YJR138W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Z49638 Genomic DNA. Translation: CAA89670.1 .
BK006943 Genomic DNA. Translation: DAA08923.1 .
PIRi S57161.
RefSeqi NP_012672.1. NM_001181796.2.

3D structure databases

ProteinModelPortali P47170.
SMRi P47170. Positions 1206-1271.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33894. 34 interactions.
DIPi DIP-2589N.
IntActi P47170. 21 interactions.
MINTi MINT-423965.
STRINGi 4932.YJR138W.

Proteomic databases

MaxQBi P47170.
PaxDbi P47170.
PeptideAtlasi P47170.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR138W ; YJR138W ; YJR138W .
GeneIDi 853603.
KEGGi sce:YJR138W.

Organism-specific databases

CYGDi YJR138w.
SGDi S000003899. IML1.

Phylogenomic databases

eggNOGi NOG235380.
GeneTreei ENSGT00390000016559.
HOGENOMi HOG000093241.
OMAi SMSNEIH.
OrthoDBi EOG7M3J7T.

Enzyme and pathway databases

BioCyci YEAST:G3O-31754-MONOMER.

Miscellaneous databases

NextBioi 974433.
PROi P47170.

Gene expression databases

Genevestigatori P47170.

Family and domain databases

Gene3Di 1.10.10.10. 1 hit.
InterProi IPR000591. DEP_dom.
IPR027244. IML1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view ]
PANTHERi PTHR13179. PTHR13179. 1 hit.
Pfami PF00610. DEP. 1 hit.
PF12257. DUF3608. 1 hit.
[Graphical view ]
SMARTi SM00049. DEP. 1 hit.
[Graphical view ]
PROSITEi PS50186. DEP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  4. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  5. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  6. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680 AND SER-737, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae."
    Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C.
    Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.

Entry informationi

Entry nameiIML1_YEAST
AccessioniPrimary (citable) accession number: P47170
Secondary accession number(s): D6VWV7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 11, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi