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P47170 (IML1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar membrane-associated protein IML1
Alternative name(s):
Increased minichromosome loss protein 1
SEH-associated protein 1
Gene names
Name:IML1
Synonyms:SEA1
Ordered Locus Names:YJR138W
ORF Names:J2129
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1584 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the SEA complex which coats the vacuolar membrane and is involved in intracellular trafficking, autophagy, response to nitrogen starvation, and amino acid biogenesis. Ref.8

Subunit structure

Component of the SEA complex composed of at least IML1/SEA1, RTC1/SEA2, MTC5/SEA3, NPR2, NPR3, SEA4, SEC13 and SEH1. Ref.8

Subcellular location

Vacuole membrane; Peripheral membrane protein Ref.3 Ref.8.

Miscellaneous

Present with 279 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the IML1 family.

Contains 1 DEP domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

VAC8P399681EBI-25710,EBI-20212

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 15841584Vacuolar membrane-associated protein IML1
PRO_0000203123

Regions

Domain1198 – 127376DEP

Amino acid modifications

Modified residue6801Phosphoserine Ref.5 Ref.6 Ref.7
Modified residue7371Phosphoserine Ref.6

Sequences

Sequence LengthMass (Da)Tools
P47170 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: BB8E73FF5B70CCDF

FASTA1,584181,949
        10         20         30         40         50         60 
MFAKLHGKKQ RPISSINSQT PRTSNTTHAN SISLSSGNLI VGSNRNLRQK KEQFGSQQRA 

        70         80         90        100        110        120 
SGRKLISNKE NDDNVNNGGD NNYDNGERVH RHHIPGLKIK AYQAELGYHE SRFSENLVML 

       130        140        150        160        170        180 
NLVEFPDIKP GDLVELKTYH KNPSASNGDK KIYFIAKDFD GETKRRAKTS NVSILSGQLQ 

       190        200        210        220        230        240 
TLLDLPSRSR IWIKLKPNKF DLQADVVEFN IKDCLLNRGD MWVLSSKLVD TCVFMDQRLA 

       250        260        270        280        290        300 
FLDSIRGTIK GIYRNGKKIV SGYIGEQTRI IFRSESARLI FLIQITDEMW NFEETGEQLF 

       310        320        330        340        350        360 
QKMVNSFFPK IFKKWKDVDT HHTITIAFAI SMDLSDTSFK DLTPGESLKN SQDYFRIVVD 

       370        380        390        400        410        420 
QVSIIHWVDI METLREEFME IRKDLLNKQT DKGYSVANGR FSPVIKSNFL ELVNFATTIL 

       430        440        450        460        470        480 
TDPFKQLDLR HTTTHVMIIS PGSGLFDVDY SLLRLTGKKL LSLEMTMDLI CLSKAPLHIV 

       490        500        510        520        530        540 
PLFRYRDFEN KLHHCVPLWL SVFFWNDHDK KSNSEWTPRC KIYDLQMMGI TENELIREVD 

       550        560        570        580        590        600 
VEYLQLNKKV KSLSEFMNDY DKNAFEVKIL CAGSNTKQSK KLNSKFDTVF ENDVVVKARK 

       610        620        630        640        650        660 
IPATATTTHG NTKFIWRGPK VALPAIKDIQ KPNVIPDLSI KTIEASFYDD CNTTNDKIST 

       670        680        690        700        710        720 
PTTSNNDNLE MNDSLVSVRS ADNQNTSLAL DSLKGLSKRN SLKDFTQRVI TKFISNIDTS 

       730        740        750        760        770        780 
KNKKIKSTLL RDDVDNSPLG SNTPLPSSES KISGLKLQQK GLADENVISK RGNLIIKKNL 

       790        800        810        820        830        840 
SIFGLPSNEI MSGSPSSYLG SSHTRTSSKL SNMSDKAAFI TEGQKSKHDD SNTYSLTQQL 

       850        860        870        880        890        900 
KHRISETWVD IKSPSIPVSS EFANELLPIR WKDVWPKYVA RKYSKWRSFT TPAELPITIS 

       910        920        930        940        950        960 
DFPSKDDFDR NFIFRNHSVT LNTDQEQYNQ TYKDLLRDMI YMRLLTGFQI CVGRQVEKIE 

       970        980        990       1000       1010       1020 
LSRESGESET VVNKYLDFNQ NDAFKLYLMI DSEIHRITCS SSGIIDVERY LRKDEANLFD 

      1030       1040       1050       1060       1070       1080 
QVPSYIPLVK TRYESSFRDA MIDPLHVKRE SLNWNQIDQV LAGYGDNLID RKWHGFRAKY 

      1090       1100       1110       1120       1130       1140 
VVLPTDIPPN TYSMVINGKS ETLNPEEIRV EGLRRLIGSI TRSRLRTEKE KKGRKTKREE 

      1150       1160       1170       1180       1190       1200 
IQPEVMFYTG PLYNFINEQQ TSLESSAINF KDSIFVNDNN LLNRNVELSK LAYQIQRGED 

      1210       1220       1230       1240       1250       1260 
RITLVNRKWH WKKHEKCFVG SEMVNWLIRN FSDIDTREDA IKYGQKVMKE GLFVHVLNKH 

      1270       1280       1290       1300       1310       1320 
NFLDGHYFYQ FSPEYVMDTN KLEKTNSHRS TLSDPKQMLR KASTGSSNDP SAMTPFSSVV 

      1330       1340       1350       1360       1370       1380 
PAISASNASV ADAKEPSRPI LMLSNSLVID VDPAGKSSKQ ESCTVHYDRV HNPDHCFHIR 

      1390       1400       1410       1420       1430       1440 
LEWLTTTPKL IDDLVGNWSR LCERYGLKMI EIPWEELCTI PSVNPFHSFV EIKLAINPWE 

      1450       1460       1470       1480       1490       1500 
DPEFKDRELF AKSKFYYHVY LLKASGFLLD NRASKFLQNQ DIEFDIMYSW GKPQFKYVQY 

      1510       1520       1530       1540       1550       1560 
IHHTGAYVAE LRENGCLFLA PNNIYISRVN PGNIIGKIHS ASSSSLDAQK VILNFKSTCL 

      1570       1580 
DYQKLRSIFL DAKEMWITGK IVED 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[4]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[6]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680 AND SER-737, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-680, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[8]"A conserved coatomer-related complex containing Sec13 and Seh1 dynamically associates with the vacuole in Saccharomyces cerevisiae."
Dokudovskaya S., Waharte F., Schlessinger A., Pieper U., Devos D.P., Cristea I.M., Williams R., Salamero J., Chait B.T., Sali A., Field M.C., Rout M.P., Dargemont C.
Mol. Cell. Proteomics 10:M110.006478.1-M110.006478.17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION, IDENTIFICATION IN THE SEA COMPLEX, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49638 Genomic DNA. Translation: CAA89670.1.
BK006943 Genomic DNA. Translation: DAA08923.1.
PIRS57161.
RefSeqNP_012672.1. NM_001181796.2.

3D structure databases

ProteinModelPortalP47170.
SMRP47170. Positions 1206-1271.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33894. 34 interactions.
DIPDIP-2589N.
IntActP47170. 21 interactions.
MINTMINT-423965.
STRING4932.YJR138W.

Proteomic databases

PaxDbP47170.
PeptideAtlasP47170.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR138W; YJR138W; YJR138W.
GeneID853603.
KEGGsce:YJR138W.

Organism-specific databases

CYGDYJR138w.
SGDS000003899. IML1.

Phylogenomic databases

eggNOGNOG235380.
GeneTreeENSGT00390000016559.
HOGENOMHOG000093241.
OMASMSNEIH.
OrthoDBEOG7M3J7T.

Enzyme and pathway databases

BioCycYEAST:G3O-31754-MONOMER.

Gene expression databases

GenevestigatorP47170.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
InterProIPR000591. DEP_dom.
IPR027244. IML1.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR13179:SF8. PTHR13179:SF8. 1 hit.
PfamPF00610. DEP. 1 hit.
PF12257. DUF3608. 1 hit.
[Graphical view]
SMARTSM00049. DEP. 1 hit.
[Graphical view]
PROSITEPS50186. DEP. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974433.
PROP47170.

Entry information

Entry nameIML1_YEAST
AccessionPrimary (citable) accession number: P47170
Secondary accession number(s): D6VWV7
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families