Sulfite reductase [NADPH] subunit beta - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47169 (MET5_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 29, 2013. Version 121. History...

Clusters with 100%, 90%, 50% identity |

Documents (4) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sulfite reductase [NADPH] subunit beta
EC=1.8.1.2

Alternative name(s):
Extracellular mutant protein 17

Gene names

Name:	MET5
Synonyms:	ECM17
Ordered Locus Names:	YJR137C
ORF Names:	J2126

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1442 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the reduction of sulfite to sulfide, one of several activities required for the biosynthesis of L-cysteine from sulfate.
Catalytic activity	H₂S + 3 NADP⁺ + 3 H₂O = sulfite + 3 NADPH.
Cofactor	Binds 1 siroheme per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Sulfur metabolism; hydrogen sulfide biosynthesis; hydrogen sulfide from sulfite (NADPH route): step 1/1.
Subunit structure	Alpha(2)-beta₂. The alpha component is a flavoprotein, the beta component is a hemoprotein.
Subcellular location	Cytoplasm Ref.6.
Miscellaneous	Present with 2900 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the nitrite and sulfite reductase 4Fe-4S domain family. Contains 1 flavodoxin-like domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	4Fe-4S Heme Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cysteine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW hydrogen sulfide biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW sulfate assimilation Inferred from mutant phenotype PubMed 19236486. Source: SGD sulfur amino acid biosynthetic process Inferred from mutant phenotype PubMed 1479340. Source: SGD
Cellular_component	cytoplasm Inferred from direct assay Ref.6. Source: SGD sulfite reductase complex (NADPH) Inferred from direct assay Ref.3 PubMed 9003798. Source: SGD
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW FMN binding Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: InterPro sulfite reductase (NADPH) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1442

1442

Sulfite reductase [NADPH] subunit beta

PRO_0000199968

Regions

Domain

682 – 831

150

Flavodoxin-like

Sites

Metal binding

1300

Iron-sulfur (4Fe-4S) By similarity

Metal binding

1306

Iron-sulfur (4Fe-4S) By similarity

Metal binding

1345

Iron-sulfur (4Fe-4S) By similarity

Metal binding

1349

Iron (siroheme axial ligand) By similarity

Metal binding

1349

Iron-sulfur (4Fe-4S) By similarity

Amino acid modifications

Modified residue

246

Phosphoserine Ref.8

Modified residue

1055

Phosphoserine Ref.8

Modified residue

1298

Phosphoserine Ref.8

Modified residue

1299

Phosphoserine Ref.8

Modified residue

1438

Phosphoserine Ref.8

Modified residue

1442

Phosphotyrosine Ref.8

Sequences

Sequence

Length

Mass (Da)

Tools

P47169 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: E34695088BA9FE94
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FASTA

1,442

161,219

        10         20         30         40         50         60 
MTASDLLTLP QLLAQYSSSA PQNKVFYTTS TKNSHSSFKG LESVATDATH LLNNQDPLNT 

        70         80         90        100        110        120 
IKDQLSKDIL TTVFTDETTL VKSIHHLYSL PNKLPLVITV DLNLQDYSAI PALKDLSFPI 

       130        140        150        160        170        180 
LISSDLQTAI SNADSSYKIA TSSLTPVFHF LNLEKIGTST AIEQDIDFPT LEIANEETKV 

       190        200        210        220        230        240 
ALSEATDSLT NFELVKGKES ITTVIVNLSP YDAEFSSVLP SNVGLIKIRV YRPWNFSKFL 

       250        260        270        280        290        300 
EILPSSVTKI AVLQGVSKKS QSNEFQPFLL DFFGNFNELV SRNIEQVVLT NIGNVNDYGN 

       310        320        330        340        350        360 
VINTVISNIN KKEPDNNLFL GESNEKAEEQ AEVTQLISSV KKVVNLEDAY IKVLKQLFSS 

       370        380        390        400        410        420 
NLQILNQFSS ETIEPSNPEF GFGRFLKQEA QREELISLAK TSLDPSLYLS EDANKIVQLL 

       430        440        450        460        470        480 
SKWLSFNGRD LDEAQLQEAN ATGLEIFQLL QSNQDSSTVL KFLKIAPTSD SFIFKSSWLI 

       490        500        510        520        530        540 
GSDAWSYDLG HSGIQQVLSS RKNINVLLID SEPYDHRKQN QDRKKDVGLY AMNYYSAYVA 

       550        560        570        580        590        600 
SVAVYASYTQ LLTAIIEASK YNGPSIVLAY LPYNSENDTP LEVLKETKNA VESGYWPLYR 

       610        620        630        640        650        660 
FNPVYDDPST DKEAFSLDSS VIRKQLQDFL DRENKLTLLT RKDPSLSRNL KQSAGDALTR 

       670        680        690        700        710        720 
KQEKRSKAAF DQLLEGLSGP PLHVYYASDG GNAANLAKRL AARASARGLK ATVLSMDDII 

       730        740        750        760        770        780 
LEELPGEENV VFITSTAGQG EFPQDGKSFW EALKNDTDLD LASLNVAVFG LGDSEYWPRK 

       790        800        810        820        830        840 
EDKHYFNKPS QDLFKRLELL SAKALIPLGL GDDQDADGFQ TAYSEWEPKL WEALGVSGAA 

       850        860        870        880        890        900 
VDDEPKPVTN EDIKRESNFL RGTISENLKD TSSGGVTHAN EQLMKFHGIY TQDDRDIREI 

       910        920        930        940        950        960 
RKSQGLEPYY MFMARARLPG GKTTPQQWLA LDHLSDTSGN GTLKLTTRAT FQIHGVLKKN 

       970        980        990       1000       1010       1020 
LKHTLRGMNA VLMDTLAAAG DVNRNVMVSA LPTNAKVHQQ IADMGKLISD HFLPKTTAYH 

      1030       1040       1050       1060       1070       1080 
EVWLEGPEEQ DDDPSWPSIF ENRKDGPRKK KTLVSGNALV DIEPIYGPTY LPRKFKFNIA 

      1090       1100       1110       1120       1130       1140 
VPPYNDVDVL SIDVGLVAIV NPETQIVEGY NVFVGGGMGT THNNKKTYPR LGSCLGFVKT 

      1150       1160       1170       1180       1190       1200 
EDIIPPLEGI VIVQRDHGDR KDRKHARLKY TVDDMGVEGF KQKVEEYWGK KFEPERPFEF 

      1210       1220       1230       1240       1250       1260 
KSNIDYFGWI KDETGLNHFT AFIENGRVED TPDLPQKTGI RKVAEYMLKT NSGHFRLTGN 

      1270       1280       1290       1300       1310       1320 
QHLVISNITD EHVAGIKSIL KTYKLDNTDF SGLRLSSSSC VGLPTCGLAF AESERFLPDI 

      1330       1340       1350       1360       1370       1380 
ITQLEDCLEE YGLRHDSIIM RMTGCPNGCS RPWLGELALV GKAPHTYNLM LGGGYLGQRL 

      1390       1400       1410       1420       1430       1440 
NKLYKANVKD EEIVDYIKPL FKRYALEREE GEHFGDFCIR VGIIKPTTEG KYFHEDVSED 


AY

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References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Studies on yeast sulfite reductase. IV. Structure and steady-state kinetics." Kobayashi K., Yoshimoto A. Biochim. Biophys. Acta 705:348-356(1982) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Linking genome and proteome by mass spectrometry: large-scale identification of yeast proteins from two dimensional gels." Shevchenko A., Jensen O.N., Podtelejnikov A.V., Sagliocco F., Wilm M., Vorm O., Mortensen P., Shevchenko A., Boucherie H., Mann M. Proc. Natl. Acad. Sci. U.S.A. 93:14440-14445(1996) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[5]	"Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae." Lussier M., White A.-M., Sheraton J., di Paolo T., Treadwell J., Southard S.B., Horenstein C.I., Chen-Weiner J., Ram A.F.J., Kapteyn J.C., Roemer T.W., Vo D.H., Bondoc D.C., Hall J., Zhong W.-W., Sdicu A.-M., Davies J., Klis F.M., Robbins P.W., Bussey H. Genetics 147:435-450(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-246; SER-1055; SER-1298; SER-1299; SER-1438 AND TYR-1442, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49637 Genomic DNA. Translation: CAA89669.1. BK006943 Genomic DNA. Translation: DAA08922.1.
PIR	S57160.
RefSeq	NP_116579.1. NM_001181795.1.
3D structure databases
ProteinModelPortal	P47169.
SMR	P47169. Positions 347-834, 886-1429.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47169. 8 interactions.
MINT	MINT-4492135.
Proteomic databases
PaxDb	P47169.
PeptideAtlas	P47169.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR137C; YJR137C; YJR137C.
GeneID	853602.
KEGG	sce:YJR137C.
Organism-specific databases
CYGD	YJR137c.
SGD	S000003898. MET5.
Phylogenomic databases
eggNOG	COG0674.
HOGENOM	HOG000167149.
KO	K00381.
OMA	QGEFPQN.
OrthoDB	EOG43BQWZ.
Enzyme and pathway databases
BioCyc	YEAST:MONOMER3O-22.
UniPathway	UPA00140; UER00207.
Gene expression databases
ArrayExpress	P47169.
Genevestigator	P47169.
GermOnline	YJR137C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	3.40.50.920. 1 hit. 3.90.480.10. 2 hits.
InterPro	IPR001094. Flavdoxin. IPR008254. Flavodoxin/NO_synth. IPR005117. NiRdtase/SiRdtase_haem-b_fer. IPR006067. NO2/SO3_Rdtase_4Fe4S_dom. IPR006066. NO2/SO3_Rdtase_FeS/sirohaem_BS. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. [Graphical view]
Pfam	PF00258. Flavodoxin_1. 1 hit. PF01077. NIR_SIR. 1 hit. PF03460. NIR_SIR_ferr. 2 hits. [Graphical view]
PRINTS	PR00369. FLAVODOXIN. PR00397. SIROHAEM.
SUPFAM	SSF55124. NiR_SiRalpha_1/3. 2 hits. SSF52922. Transketo_C_like. 1 hit.
PROSITE	PS50902. FLAVODOXIN_LIKE. 1 hit. PS00365. NIR_SIR. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974430.

Entry information

Entry name

MET5_YEAST

Accession

Primary (citable) accession number: P47169
Secondary accession number(s): D6VWV6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 121 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents