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Protein

Central kinetochore subunit MCM22

Gene

MCM22

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the central kinetochore, which mediates the attachment of the centromere to the mitotic spindle by forming essential interactions between the microtubule-associated outer kinetochore proteins and the centromere-associated inner kinetochore proteins. Required for establishing bipolar spindle-microtubule attachments and proper chromosome segregation.1 Publication

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • establishment of mitotic sister chromatid cohesion Source: SGD
  • meiotic cell cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Meiosis, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31752-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Central kinetochore subunit MCM22
Alternative name(s):
Minichromosome maintenance protein 22
Gene namesi
Name:MCM22
Ordered Locus Names:YJR135C
ORF Names:J2122
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR135C.
SGDiS000003896. MCM22.

Subcellular locationi

GO - Cellular componenti

  • condensed nuclear chromosome kinetochore Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239Central kinetochore subunit MCM22PRO_0000096283Add
BLAST

Proteomic databases

MaxQBiP47167.

Interactioni

Subunit structurei

Heterotrimer. CTF3, MCM16 and MCM22 form the CTF3 kinetochore subcomplex. The CTF3 subcomplex is part of a larger central kinetochore CTF19 complex, which is composed of at least AME1, CHL4, CTF3, CTF19, IML3, MCM16, MCM21, MCM22, NKP1, NKP2 and OKP1. Interacts with CTF19.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CTF3Q127482EBI-25691,EBI-30457
MCM16Q122627EBI-25691,EBI-31487

Protein-protein interaction databases

BioGridi33890. 98 interactions.
DIPiDIP-1431N.
IntActiP47167. 8 interactions.
MINTiMINT-393689.

Structurei

3D structure databases

ProteinModelPortaliP47167.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000000826.
InParanoidiP47167.
OMAiSERADPI.
OrthoDBiEOG741ZCV.

Sequencei

Sequence statusi: Complete.

P47167-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDVEKDVLDV YIKNLENQIG NKRYFLKQAQ GAIDEITKRS LDTEGKPVNS
60 70 80 90 100
EVFTELLRKP MFFSERADPI GFSLTSNFLS LRAQSSSEWL SLMNDQSVDQ
110 120 130 140 150
KAMLLLQNNI NSDLKELLRK LQHQMTIMDS KKQDHAHIRT RKARNKELWD
160 170 180 190 200
SLADFLKGYL VPNLDDNDES IDSLTNEVML LMKRLIEHDL NLTLNDFSSK
210 220 230
TIPIYRLLLR ANIITVIEGS TNPGTKYIKL IDFNETSLT
Length:239
Mass (Da):27,568
Last modified:February 1, 1996 - v1
Checksum:i342C2A95CDBA4878
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49635 Genomic DNA. Translation: CAA89666.1.
BK006943 Genomic DNA. Translation: DAA08919.1.
PIRiS57158.
RefSeqiNP_012669.1. NM_001181793.1.

Genome annotation databases

EnsemblFungiiYJR135C; YJR135C; YJR135C.
GeneIDi853599.
KEGGisce:YJR135C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49635 Genomic DNA. Translation: CAA89666.1.
BK006943 Genomic DNA. Translation: DAA08919.1.
PIRiS57158.
RefSeqiNP_012669.1. NM_001181793.1.

3D structure databases

ProteinModelPortaliP47167.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33890. 98 interactions.
DIPiDIP-1431N.
IntActiP47167. 8 interactions.
MINTiMINT-393689.

Proteomic databases

MaxQBiP47167.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR135C; YJR135C; YJR135C.
GeneIDi853599.
KEGGisce:YJR135C.

Organism-specific databases

EuPathDBiFungiDB:YJR135C.
SGDiS000003896. MCM22.

Phylogenomic databases

HOGENOMiHOG000000826.
InParanoidiP47167.
OMAiSERADPI.
OrthoDBiEOG741ZCV.

Enzyme and pathway databases

BioCyciYEAST:G3O-31752-MONOMER.

Miscellaneous databases

PROiP47167.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "MCM21 and MCM22, two novel genes of the yeast Saccharomyces cerevisiae are required for chromosome transmission."
    Poddar A., Roy N., Sinha P.
    Mol. Microbiol. 31:349-360(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  4. "Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p."
    Cheeseman I.M., Anderson S., Jwa M., Green E.M., Kang J.-S., Yates J.R. III, Chan C.S.M., Drubin D.G., Barnes G.
    Cell 111:163-172(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, COMPONENT OF CTF19 COMPLEX.
  5. "Ctf3p, the Mis6 budding yeast homolog, interacts with Mcm22p and Mcm16p at the yeast outer kinetochore."
    Measday V., Hailey D.W., Pot I., Givan S.A., Hyland K.M., Cagney G., Fields S., Davis T.N., Hieter P.
    Genes Dev. 16:101-113(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CTF3; CTF19 AND MCM16, SUBCELLULAR LOCATION.
  6. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMCM22_YEAST
AccessioniPrimary (citable) accession number: P47167
Secondary accession number(s): D6VWV3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1026 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.