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Protein

Cystathionine gamma-synthase

Gene

STR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

O4-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative cystathionine gamma-synthase YML082W (YML082W), Cystathionine gamma-synthase (STR2), Putative cystathionine gamma-synthase YLL058W (YLL058W)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cystathionine gamma-synthase activity Source: SGD
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfur compound metabolic process Source: SGD
  • transsulfuration Source: SGD

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Methionine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YJR130C-MONOMER
YEAST:YJR130C-MONOMER
UniPathwayiUPA00051; UER00077

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase (EC:2.5.1.48)
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Sulfur transfer protein 2
Gene namesi
Name:STR2
Ordered Locus Names:YJR130C
ORF Names:J2063
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR130C
SGDiS000003891 STR2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147811 – 639Cystathionine gamma-synthaseAdd BLAST639

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei443N6-(pyridoxal phosphate)lysineSequence analysis1

Proteomic databases

MaxQBiP47164
PaxDbiP47164
PRIDEiP47164

PTM databases

iPTMnetiP47164

Interactioni

Protein-protein interaction databases

BioGridi33885, 106 interactors
DIPiDIP-5049N
IntActiP47164, 1 interactor
STRINGi4932.YJR130C

Structurei

3D structure databases

ProteinModelPortaliP47164
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016853
HOGENOMiHOG000193384
InParanoidiP47164
KOiK01739
OMAiYVLLAHY
OrthoDBiEOG092C18EA

Family and domain databases

Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PfamiView protein in Pfam
PF01053 Cys_Met_Meta_PP, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00868 CYS_MET_METAB_PP, 1 hit

Sequencei

Sequence statusi: Complete.

P47164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISRTIGESI PPNTKHAVSV CLPTWEATVG YEEGESSIIN SLTTGYPRFF
60 70 80 90 100
IHKSIKKLCE ILSAKYSMED EACLCFPSYK VANRCREFIK VKTGLSTKVR
110 120 130 140 150
ILQLCTPKPM NQEEKLWRRE CKITVVFVDQ EIFPVMKQYW QHSGEIVSSR
160 170 180 190 200
MAEYILHELQ VKDNLKKMET VDNGKKFMTE DENRVNEEYI ETRFGRNLNF
210 220 230 240 250
LAADKAKYLI RKRIATKVVE KIDSEGLSDL FSFEHYNESN GPFNVGSGEA
260 270 280 290 300
LDDDQLNSDI PAETITSMGE SGSNSTFENT ATDDLKFHVN PNTDVYLFPS
310 320 330 340 350
GMASIFTAHR LLLNFDAKRL SRSSSRQDKL IGYGPPFKKT VMFGFPYTDT
360 370 380 390 400
LSILRKFNHT HFLGQGDSTS MNALKNILHS GEQILAVFIE APSNPLLKMG
410 420 430 440 450
DLQELKRLSD LYSFYIVVDE TVGGFVNIDV LPYADIVCSS LTKIFSGDSN
460 470 480 490 500
VIAGSLVLNP RGKIYEFARK FMKTEDGYED CLWCEDALCL ERNSRDFVER
510 520 530 540 550
TIKVNTNTDI LLKRVLLPQV GKLFKKIYYP SLTSEDTKRN YDSVMSTKDG
560 570 580 590 600
GYGGLFSLTF FNIEEAKKFF NNLELCKGPS LGTNFTLACP YAIIAHYQEL
610 620 630
DEVAQYGVET NLVRVSVGLE NSDVLCNVFQ RAIEKALGE
Length:639
Mass (Da):72,351
Last modified:February 1, 1996 - v1
Checksum:iE94FC84295B538A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49630 Genomic DNA Translation: CAA89661.1
AY692979 Genomic DNA Translation: AAT92998.1
BK006943 Genomic DNA Translation: DAA08914.1
PIRiS57153
RefSeqiNP_012664.1, NM_001181788.1

Genome annotation databases

EnsemblFungiiYJR130C; YJR130C; YJR130C
GeneIDi853594
KEGGisce:YJR130C

Similar proteinsi

Entry informationi

Entry nameiMET7_YEAST
AccessioniPrimary (citable) accession number: P47164
Secondary accession number(s): D6VWU8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 146 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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