Reviewed, UniProtKB/Swiss-Prot P47164 (MET7_YEAST)

Names and origin

Protein names

Recommended name:
    Cystathionine gamma-synthase
    EC=2.5.1.48
Alternative name(s):
    O-succinylhomoserine (thiol)-lyase
    Sulfur transfer protein 2

Gene names

Name:	STR2
Ordered Locus Names:	YJR130C
ORF Names:	J2063

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway.
Subcellular location	Cytoplasm. Nucleus. Ref.4
Miscellaneous	Present with 2070 molecules/cell in log phase SD medium. Ref.5
Sequence similarities	Belongs to the trans-sulfuration enzymes family. MET7 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm Nucleus
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW transsulfuration Ref.3 Inferred from mutant phenotype. Source: SGD
Cellular component	cytoplasm Ref.4 Inferred from direct assay. Source: SGD nucleus Ref.4 Inferred from direct assay. Source: SGD
Molecular function	cystathionine gamma-synthase activity Ref.3 Inferred from mutant phenotype. Source: SGD pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 639	639	Cystathionine gamma-synthase		PRO_0000114781
Amino acid modifications
	Modified residue	443	1	N6-(pyridoxal phosphate)lysine Potential

Sequences

	Sequence		Length	Mass (Da)
	P47164-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: E94FC84295B538A3	FASTA	639	72,351
	10 20 30 40 50 60 MISRTIGESI PPNTKHAVSV CLPTWEATVG YEEGESSIIN SLTTGYPRFF IHKSIKKLCE 70 80 90 100 110 120 ILSAKYSMED EACLCFPSYK VANRCREFIK VKTGLSTKVR ILQLCTPKPM NQEEKLWRRE 130 140 150 160 170 180 CKITVVFVDQ EIFPVMKQYW QHSGEIVSSR MAEYILHELQ VKDNLKKMET VDNGKKFMTE 190 200 210 220 230 240 DENRVNEEYI ETRFGRNLNF LAADKAKYLI RKRIATKVVE KIDSEGLSDL FSFEHYNESN 250 260 270 280 290 300 GPFNVGSGEA LDDDQLNSDI PAETITSMGE SGSNSTFENT ATDDLKFHVN PNTDVYLFPS 310 320 330 340 350 360 GMASIFTAHR LLLNFDAKRL SRSSSRQDKL IGYGPPFKKT VMFGFPYTDT LSILRKFNHT 370 380 390 400 410 420 HFLGQGDSTS MNALKNILHS GEQILAVFIE APSNPLLKMG DLQELKRLSD LYSFYIVVDE 430 440 450 460 470 480 TVGGFVNIDV LPYADIVCSS LTKIFSGDSN VIAGSLVLNP RGKIYEFARK FMKTEDGYED 490 500 510 520 530 540 CLWCEDALCL ERNSRDFVER TIKVNTNTDI LLKRVLLPQV GKLFKKIYYP SLTSEDTKRN 550 560 570 580 590 600 YDSVMSTKDG GYGGLFSLTF FNIEEAKKFF NNLELCKGPS LGTNFTLACP YAIIAHYQEL 610 620 630 DEVAQYGVET NLVRVSVGLE NSDVLCNVFQ RAIEKALGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae." Hansen J., Johannesen P.F. Mol. Gen. Genet. 263:535-542(2000) [PubMed: 10821189] [Abstract] Cited for: IDENTIFICATION.
[4]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49630 Genomic DNA. Translation: CAA89661.1. AY692979 Genomic DNA. Translation: AAT92998.1.
PIR	S57153.
RefSeq	NP_012664.1.
3D structure databases
HSSP	HSSP built from PDB template 1GC0 based on UniProtKB P13254.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:5049N.
IntAct	P47164. 2 interactions.
STRING	P47164.
Genome annotation databases
Ensembl	YJR130C; YJR130C; YJR130C; Saccharomyces cerevisiae. [Genome view]
GeneID	853594.
GenomeReviews	Gene locus YJR130C in contig Y13136_GR.
KEGG	sce:YJR130C.
NMPDR	fig|4932.3.peg.3639.
Organism-specific databases
CYGD	YJR130c.
SGD	S000003891. STR2.
Phylogenomic databases
HOGENOM	P47164.
OMA	ALFCEFP.
Enzyme and pathway databases
BioCyc	MetaCyc:MON-390.
BRENDA	2.5.1.48. 250.
Gene expression databases
ArrayExpress	P47164.
Genevestigator	P47164.
GermOnline	YJR130C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974406.

Entry information

Entry name

MET7_YEAST

Accession

Primary (citable) accession number: P47164

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 3, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)