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Protein

Cystathionine gamma-synthase

Gene

STR2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

Pathwayi: L-methionine biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine.
Proteins known to be involved in this subpathway in this organism are:
  1. Putative cystathionine gamma-synthase YML082W (YML082W), Cystathionine gamma-synthase (STR2), Putative cystathionine gamma-synthase YLL058W (YLL058W)
This subpathway is part of the pathway L-methionine biosynthesis via de novo pathway, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cystathionine from O-succinyl-L-homoserine, the pathway L-methionine biosynthesis via de novo pathway and in Amino-acid biosynthesis.

GO - Molecular functioni

  • cystathionine gamma-synthase activity Source: SGD
  • pyridoxal phosphate binding Source: InterPro

GO - Biological processi

  • methionine biosynthetic process Source: UniProtKB-KW
  • sulfur compound metabolic process Source: SGD
  • transsulfuration Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-390.
YEAST:YJR130C-MONOMER.
UniPathwayiUPA00051; UER00077.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase (EC:2.5.1.48)
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Sulfur transfer protein 2
Gene namesi
Name:STR2
Ordered Locus Names:YJR130C
ORF Names:J2063
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR130C.
SGDiS000003891. STR2.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 639639Cystathionine gamma-synthasePRO_0000114781Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei275 – 2751PhosphoserineBy similarity
Modified residuei443 – 4431N6-(pyridoxal phosphate)lysineSequence analysis

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47164.

PTM databases

iPTMnetiP47164.

Interactioni

Protein-protein interaction databases

BioGridi33885. 57 interactions.
DIPiDIP-5049N.
IntActiP47164. 1 interaction.
MINTiMINT-570168.

Structurei

3D structure databases

ProteinModelPortaliP47164.
SMRiP47164. Positions 367-635.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00390000016853.
HOGENOMiHOG000193384.
InParanoidiP47164.
KOiK01739.
OMAiCEFPGNP.
OrthoDBiEOG7GFBDM.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47164-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISRTIGESI PPNTKHAVSV CLPTWEATVG YEEGESSIIN SLTTGYPRFF
60 70 80 90 100
IHKSIKKLCE ILSAKYSMED EACLCFPSYK VANRCREFIK VKTGLSTKVR
110 120 130 140 150
ILQLCTPKPM NQEEKLWRRE CKITVVFVDQ EIFPVMKQYW QHSGEIVSSR
160 170 180 190 200
MAEYILHELQ VKDNLKKMET VDNGKKFMTE DENRVNEEYI ETRFGRNLNF
210 220 230 240 250
LAADKAKYLI RKRIATKVVE KIDSEGLSDL FSFEHYNESN GPFNVGSGEA
260 270 280 290 300
LDDDQLNSDI PAETITSMGE SGSNSTFENT ATDDLKFHVN PNTDVYLFPS
310 320 330 340 350
GMASIFTAHR LLLNFDAKRL SRSSSRQDKL IGYGPPFKKT VMFGFPYTDT
360 370 380 390 400
LSILRKFNHT HFLGQGDSTS MNALKNILHS GEQILAVFIE APSNPLLKMG
410 420 430 440 450
DLQELKRLSD LYSFYIVVDE TVGGFVNIDV LPYADIVCSS LTKIFSGDSN
460 470 480 490 500
VIAGSLVLNP RGKIYEFARK FMKTEDGYED CLWCEDALCL ERNSRDFVER
510 520 530 540 550
TIKVNTNTDI LLKRVLLPQV GKLFKKIYYP SLTSEDTKRN YDSVMSTKDG
560 570 580 590 600
GYGGLFSLTF FNIEEAKKFF NNLELCKGPS LGTNFTLACP YAIIAHYQEL
610 620 630
DEVAQYGVET NLVRVSVGLE NSDVLCNVFQ RAIEKALGE
Length:639
Mass (Da):72,351
Last modified:February 1, 1996 - v1
Checksum:iE94FC84295B538A3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49630 Genomic DNA. Translation: CAA89661.1.
AY692979 Genomic DNA. Translation: AAT92998.1.
BK006943 Genomic DNA. Translation: DAA08914.1.
PIRiS57153.
RefSeqiNP_012664.1. NM_001181788.1.

Genome annotation databases

EnsemblFungiiYJR130C; YJR130C; YJR130C.
GeneIDi853594.
KEGGisce:YJR130C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49630 Genomic DNA. Translation: CAA89661.1.
AY692979 Genomic DNA. Translation: AAT92998.1.
BK006943 Genomic DNA. Translation: DAA08914.1.
PIRiS57153.
RefSeqiNP_012664.1. NM_001181788.1.

3D structure databases

ProteinModelPortaliP47164.
SMRiP47164. Positions 367-635.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33885. 57 interactions.
DIPiDIP-5049N.
IntActiP47164. 1 interaction.
MINTiMINT-570168.

PTM databases

iPTMnetiP47164.

Proteomic databases

MaxQBiP47164.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR130C; YJR130C; YJR130C.
GeneIDi853594.
KEGGisce:YJR130C.

Organism-specific databases

EuPathDBiFungiDB:YJR130C.
SGDiS000003891. STR2.

Phylogenomic databases

GeneTreeiENSGT00390000016853.
HOGENOMiHOG000193384.
InParanoidiP47164.
KOiK01739.
OMAiCEFPGNP.
OrthoDBiEOG7GFBDM.

Enzyme and pathway databases

UniPathwayiUPA00051; UER00077.
BioCyciMetaCyc:MONOMER-390.
YEAST:YJR130C-MONOMER.

Miscellaneous databases

NextBioi974406.
PROiP47164.

Family and domain databases

Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 2 hits.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Cysteine is essential for transcriptional regulation of the sulfur assimilation genes in Saccharomyces cerevisiae."
    Hansen J., Johannesen P.F.
    Mol. Gen. Genet. 263:535-542(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiMET7_YEAST
AccessioniPrimary (citable) accession number: P47164
Secondary accession number(s): D6VWU8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.