P47160 (ENT3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Epsin-3

Gene names

Name:	ENT3
Ordered Locus Names:	YJR125C
ORF Names:	J2048

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the recruitment of clathrin to the Golgi network and endosomes to form clathrin coated vesicles. Plays a role in the trafficking of clathrin between the Golgi network and endosomes. Binds to membranes enriched in phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) and, in association with VPS27, is involved in protein sorting at the multivesicular body (MVB). Ref.4 Ref.5
Subunit structure	Interacts with the clathrin adapter GGA2, and VPS27. Ref.4 Ref.5
Subcellular location	Cytoplasm. Golgi apparatus › trans-Golgi network membrane; Peripheral membrane protein. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein. Note: Associates with the trans Golgi network (TGN) and endosomal clathrin coats. Ref.4
Sequence similarities	Contains 1 ENTH (epsin N-terminal homology) domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasm Cytoplasmic vesicle Golgi apparatus Membrane
Ligand	Lipid-binding
PTM	Phosphoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Golgi to endosome transport Inferred from mutant phenotype Ref.4. Source: SGD actin cortical patch assembly Inferred from sequence or structural similarity PubMed 10449404. Source: SGD actin filament organization Inferred from sequence or structural similarity PubMed 10449404. Source: SGD early endosome to Golgi transport Inferred from genetic interaction PubMed 20658963. Source: SGD endocytosis Inferred from sequence or structural similarity PubMed 10449404. Source: SGD late endosome to vacuole transport via multivesicular body sorting pathway Inferred from mutant phenotype Ref.5. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: UniProtKB-SubCell actin cortical patch Traceable author statement PubMed 10652251. Source: SGD clathrin vesicle coat Inferred from direct assay Ref.4. Source: SGD
Molecular_function	phosphatidylinositol-3,5-bisphosphate binding Inferred from direct assay PubMed 14630930. Source: SGD phosphatidylinositol-3-phosphate binding Inferred from direct assay PubMed 14630930. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 408

408

Epsin-3

PRO_0000074526

Regions

Domain

24 – 157

134

ENTH

Amino acid modifications

Modified residue

203

1

Phosphoserine Ref.7 Ref.8

Modified residue

294

1

Phosphoserine Ref.8

Modified residue

320

1

Phosphothreonine Ref.8

Modified residue

365

1

Phosphoserine Ref.8

Modified residue

407

1

Phosphoserine Ref.6

Experimental info

Mutagenesis

272

1

F → A: Reduced binding to GGA2. Ref.4

Mutagenesis

275

1

F → A: Reduced binding to GGA2. Ref.4

Secondary structure

1

.

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408

Helix Strand Turn

Details...

Helix

31 – 39

9

Helix

49 – 58

10

Helix

62 – 77

16

Helix

81 – 83

3

Helix

84 – 100

17

Helix

103 – 110

8

Helix

113 – 117

5

Helix

118 – 121

4

Helix

133 – 147

15

Helix

150 – 162

13

Sequences

	Sequence		Length	Mass (Da)
	P47160 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D666ECB1C0D074FE	FASTA	408	45,091
	10 20 30 40 50 60 MSLEDTLANM SLYDAKKYFR KAQNVVFNYT EMEGKVREAT NNEPWGASST LMDQISQGTY 70 80 90 100 110 120 NFREREEILS MIFRRFTEKA GSEWRQIYKA LQLLDYLIKH GSERFIDDTR NSINLIRILE 130 140 150 160 170 180 TFHYIDSQGR DQGINVRTRV KALIELLSDD NKIRAERKKA RETAKKYKGV AGGSASADGS 190 200 210 220 230 240 LNSKAGFTST KVHGISVSAD FDSDNEDNED GSFSQNGYND NASRATSTPG QGKQEPEDFV 250 260 270 280 290 300 DFFSSESSKP SKELIQEDEK KADEEEDDDD EFSEFQSAVP VTNPANSFNL LNTSPIEGMP 310 320 330 340 350 360 ATTSSMPFYN SSTTDQGKIT PAIAEPKKVD PFSSLFSTAK ASAEAPSAPK ASQAKAAASN 370 380 390 400 PVSNSTTALS TDQDDDDEFG EMHGGAVQQE QNTNNNHTSS KEIDLLSF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif." Duncan M.C., Costaguta G., Payne G.S. Nat. Cell Biol. 5:77-81(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH GGA2, SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-272 AND PHE-275.
[5]	"Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body." Eugster A., Pecheur E.-I., Michel F., Winsor B., Letourneur F., Friant S. Mol. Biol. Cell 15:3031-3041(2004) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH VPS27.
[6]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-407, MASS SPECTROMETRY. Strain: YAL6B.
[7]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, MASS SPECTROMETRY. Strain: ADR376.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203; SER-294; THR-320 AND SER-365, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

Z49625 Genomic DNA. Translation: CAA89656.1.
AY558084 Genomic DNA. Translation: AAS56410.1.
BK006943 Genomic DNA. Translation: DAA08910.1.

PIR

S57148.

RefSeq

NP_012659.1. NM_001181783.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3ONK	X-ray	2.09	A	28-170	[»]
3ONL	X-ray	2.20	A/B	28-170	[»]

ProteinModelPortal

P47160.

SMR

P47160. Positions 28-163.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-1304N.

IntAct

P47160. 5 interactions.

MINT

MINT-400227.

STRING

4932.YJR125C.

Proteomic databases

PaxDb

P47160.

PeptideAtlas

P47160.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YJR125C; YJR125C; YJR125C.

GeneID

853589.

KEGG

sce:YJR125C.

Organism-specific databases

CYGD

YJR125c.

SGD

S000003886. ENT3.

Phylogenomic databases

eggNOG

NOG249411.

GeneTree

ENSGT00550000074611.

HOGENOM

HOG000183501.

OMA

GMIFRRF.

OrthoDB

EOG4T4H4C.

Gene expression databases

Genevestigator

P47160.

GermOnline

YJR125C. Saccharomyces cerevisiae.

Family and domain databases

Gene3D

1.25.40.90. 1 hit.

InterPro

IPR008942. ENTH_VHS.
IPR013809. Epsin-like_N.
IPR001026. Epsin_dom_N.
[Graphical view]

Pfam

PF01417. ENTH. 1 hit.
[Graphical view]

SMART

SM00273. ENTH. 1 hit.
[Graphical view]

SUPFAM

SSF48464. ENTH_VHS. 1 hit.

PROSITE

PS50942. ENTH. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

974394.

Entry information

Entry name

ENT3_YEAST

Accession

Primary (citable) accession number: P47160
Secondary accession number(s): D6VWU4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program