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Protein

Epsin-3

Gene

ENT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the recruitment of clathrin to the Golgi network and endosomes to form clathrin coated vesicles. Plays a role in the trafficking of clathrin between the Golgi network and endosomes. Binds to membranes enriched in phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) and, in association with VPS27, is involved in protein sorting at the multivesicular body (MVB).2 Publications

GO - Molecular functioni

  • clathrin binding Source: SGD
  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD
  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • actin cytoskeleton organization Source: SGD
  • actin filament organization Source: SGD
  • early endosome to Golgi transport Source: SGD
  • Golgi to endosome transport Source: SGD
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • multivesicular body sorting pathway Source: SGD
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31746-MONOMER.
ReactomeiR-SCE-8856825. Cargo recognition for clathrin-mediated endocytosis.

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-3
Gene namesi
Name:ENT3
Ordered Locus Names:YJR125C
ORF Names:J2048
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR125C.
SGDiS000003886. ENT3.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi272F → A: Reduced binding to GGA2. 1 Publication1
Mutagenesisi275F → A: Reduced binding to GGA2. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000745262 – 408Epsin-3Add BLAST407

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1
Modified residuei196PhosphoserineCombined sources1
Modified residuei198PhosphoserineCombined sources1
Modified residuei203PhosphoserineCombined sources1
Modified residuei212PhosphoserineCombined sources1
Modified residuei223PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP47160.
PRIDEiP47160.

PTM databases

iPTMnetiP47160.

Interactioni

Subunit structurei

Interacts with the clathrin adapter GGA2, and VPS27.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
VTI1Q043387EBI-25662,EBI-20519

GO - Molecular functioni

  • clathrin binding Source: SGD

Protein-protein interaction databases

BioGridi33881. 171 interactors.
DIPiDIP-1304N.
ELMiP47160.
IntActiP47160. 7 interactors.
MINTiMINT-400227.
STRINGi4932.YJR125C.

Structurei

Secondary structure

1408
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi31 – 39Combined sources9
Helixi49 – 58Combined sources10
Helixi62 – 77Combined sources16
Helixi81 – 83Combined sources3
Helixi84 – 100Combined sources17
Helixi103 – 110Combined sources8
Helixi113 – 117Combined sources5
Helixi118 – 121Combined sources4
Helixi133 – 147Combined sources15
Helixi150 – 162Combined sources13

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3ONKX-ray2.09A28-170[»]
3ONLX-ray2.20A/B28-170[»]
ProteinModelPortaliP47160.
SMRiP47160.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 157ENTHPROSITE-ProRule annotationAdd BLAST134

Phylogenomic databases

GeneTreeiENSGT00550000074611.
HOGENOMiHOG000183501.
InParanoidiP47160.
KOiK12471.
OMAiGMIFRRF.
OrthoDBiEOG092C3ZA8.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiView protein in InterPro
IPR013809. ENTH.
IPR008942. ENTH_VHS.
PfamiView protein in Pfam
PF01417. ENTH. 1 hit.
SMARTiView protein in SMART
SM00273. ENTH. 1 hit.
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiView protein in PROSITE
PS50942. ENTH. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47160-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLEDTLANM SLYDAKKYFR KAQNVVFNYT EMEGKVREAT NNEPWGASST
60 70 80 90 100
LMDQISQGTY NFREREEILS MIFRRFTEKA GSEWRQIYKA LQLLDYLIKH
110 120 130 140 150
GSERFIDDTR NSINLIRILE TFHYIDSQGR DQGINVRTRV KALIELLSDD
160 170 180 190 200
NKIRAERKKA RETAKKYKGV AGGSASADGS LNSKAGFTST KVHGISVSAD
210 220 230 240 250
FDSDNEDNED GSFSQNGYND NASRATSTPG QGKQEPEDFV DFFSSESSKP
260 270 280 290 300
SKELIQEDEK KADEEEDDDD EFSEFQSAVP VTNPANSFNL LNTSPIEGMP
310 320 330 340 350
ATTSSMPFYN SSTTDQGKIT PAIAEPKKVD PFSSLFSTAK ASAEAPSAPK
360 370 380 390 400
ASQAKAAASN PVSNSTTALS TDQDDDDEFG EMHGGAVQQE QNTNNNHTSS

KEIDLLSF
Length:408
Mass (Da):45,091
Last modified:February 1, 1996 - v1
Checksum:iD666ECB1C0D074FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49625 Genomic DNA. Translation: CAA89656.1.
AY558084 Genomic DNA. Translation: AAS56410.1.
BK006943 Genomic DNA. Translation: DAA08910.1.
PIRiS57148.
RefSeqiNP_012659.1. NM_001181783.1.

Genome annotation databases

EnsemblFungiiYJR125C; YJR125C; YJR125C.
GeneIDi853589.
KEGGisce:YJR125C.

Similar proteinsi

Entry informationi

Entry nameiENT3_YEAST
AccessioniPrimary (citable) accession number: P47160
Secondary accession number(s): D6VWU4
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 22, 2017
This is version 144 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names