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Protein

Epsin-3

Gene

ENT3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the recruitment of clathrin to the Golgi network and endosomes to form clathrin coated vesicles. Plays a role in the trafficking of clathrin between the Golgi network and endosomes. Binds to membranes enriched in phosphatidylinositol-3,5-bisphosphate (PtdIns(3,5)P2) and, in association with VPS27, is involved in protein sorting at the multivesicular body (MVB).2 Publications

GO - Molecular functioni

  • clathrin binding Source: SGD
  • phosphatidylinositol-3,5-bisphosphate binding Source: SGD
  • phosphatidylinositol-3-phosphate binding Source: SGD

GO - Biological processi

  • actin cortical patch assembly Source: SGD
  • actin cytoskeleton organization Source: SGD
  • actin filament organization Source: SGD
  • early endosome to Golgi transport Source: SGD
  • Golgi to endosome transport Source: SGD
  • late endosome to vacuole transport via multivesicular body sorting pathway Source: SGD
  • multivesicular body sorting pathway Source: SGD
  • protein transport Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

Lipid-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31746-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Epsin-3
Gene namesi
Name:ENT3
Ordered Locus Names:YJR125C
ORF Names:J2048
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR125C.
SGDiS000003886. ENT3.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: SGD
  • clathrin vesicle coat Source: SGD
  • cytosol Source: GOC
  • endosome Source: SGD
  • Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoplasmic vesicle, Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi272 – 2721F → A: Reduced binding to GGA2. 1 Publication
Mutagenesisi275 – 2751F → A: Reduced binding to GGA2. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 408407Epsin-3PRO_0000074526Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources
Modified residuei196 – 1961PhosphoserineCombined sources
Modified residuei198 – 1981PhosphoserineCombined sources
Modified residuei203 – 2031PhosphoserineCombined sources
Modified residuei212 – 2121PhosphoserineCombined sources
Modified residuei223 – 2231PhosphoserineCombined sources

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP47160.
PeptideAtlasiP47160.

PTM databases

iPTMnetiP47160.

Interactioni

Subunit structurei

Interacts with the clathrin adapter GGA2, and VPS27.2 Publications

GO - Molecular functioni

  • clathrin binding Source: SGD

Protein-protein interaction databases

BioGridi33881. 58 interactions.
DIPiDIP-1304N.
IntActiP47160. 6 interactions.
MINTiMINT-400227.

Structurei

Secondary structure

1
408
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi31 – 399Combined sources
Helixi49 – 5810Combined sources
Helixi62 – 7716Combined sources
Helixi81 – 833Combined sources
Helixi84 – 10017Combined sources
Helixi103 – 1108Combined sources
Helixi113 – 1175Combined sources
Helixi118 – 1214Combined sources
Helixi133 – 14715Combined sources
Helixi150 – 16213Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ONKX-ray2.09A28-170[»]
3ONLX-ray2.20A/B28-170[»]
ProteinModelPortaliP47160.
SMRiP47160. Positions 28-163.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 157134ENTHPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ENTH (epsin N-terminal homology) domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000074611.
HOGENOMiHOG000183501.
InParanoidiP47160.
KOiK12471.
OMAiGMIFRRF.
OrthoDBiEOG7B8SFN.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47160-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLEDTLANM SLYDAKKYFR KAQNVVFNYT EMEGKVREAT NNEPWGASST
60 70 80 90 100
LMDQISQGTY NFREREEILS MIFRRFTEKA GSEWRQIYKA LQLLDYLIKH
110 120 130 140 150
GSERFIDDTR NSINLIRILE TFHYIDSQGR DQGINVRTRV KALIELLSDD
160 170 180 190 200
NKIRAERKKA RETAKKYKGV AGGSASADGS LNSKAGFTST KVHGISVSAD
210 220 230 240 250
FDSDNEDNED GSFSQNGYND NASRATSTPG QGKQEPEDFV DFFSSESSKP
260 270 280 290 300
SKELIQEDEK KADEEEDDDD EFSEFQSAVP VTNPANSFNL LNTSPIEGMP
310 320 330 340 350
ATTSSMPFYN SSTTDQGKIT PAIAEPKKVD PFSSLFSTAK ASAEAPSAPK
360 370 380 390 400
ASQAKAAASN PVSNSTTALS TDQDDDDEFG EMHGGAVQQE QNTNNNHTSS

KEIDLLSF
Length:408
Mass (Da):45,091
Last modified:February 1, 1996 - v1
Checksum:iD666ECB1C0D074FE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49625 Genomic DNA. Translation: CAA89656.1.
AY558084 Genomic DNA. Translation: AAS56410.1.
BK006943 Genomic DNA. Translation: DAA08910.1.
PIRiS57148.
RefSeqiNP_012659.1. NM_001181783.1.

Genome annotation databases

EnsemblFungiiYJR125C; YJR125C; YJR125C.
GeneIDi853589.
KEGGisce:YJR125C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49625 Genomic DNA. Translation: CAA89656.1.
AY558084 Genomic DNA. Translation: AAS56410.1.
BK006943 Genomic DNA. Translation: DAA08910.1.
PIRiS57148.
RefSeqiNP_012659.1. NM_001181783.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3ONKX-ray2.09A28-170[»]
3ONLX-ray2.20A/B28-170[»]
ProteinModelPortaliP47160.
SMRiP47160. Positions 28-163.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33881. 58 interactions.
DIPiDIP-1304N.
IntActiP47160. 6 interactions.
MINTiMINT-400227.

PTM databases

iPTMnetiP47160.

Proteomic databases

MaxQBiP47160.
PeptideAtlasiP47160.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR125C; YJR125C; YJR125C.
GeneIDi853589.
KEGGisce:YJR125C.

Organism-specific databases

EuPathDBiFungiDB:YJR125C.
SGDiS000003886. ENT3.

Phylogenomic databases

GeneTreeiENSGT00550000074611.
HOGENOMiHOG000183501.
InParanoidiP47160.
KOiK12471.
OMAiGMIFRRF.
OrthoDBiEOG7B8SFN.

Enzyme and pathway databases

BioCyciYEAST:G3O-31746-MONOMER.

Miscellaneous databases

PROiP47160.

Family and domain databases

Gene3Di1.25.40.90. 1 hit.
InterProiIPR013809. ENTH.
IPR008942. ENTH_VHS.
[Graphical view]
PfamiPF01417. ENTH. 1 hit.
[Graphical view]
SMARTiSM00273. ENTH. 1 hit.
[Graphical view]
SUPFAMiSSF48464. SSF48464. 1 hit.
PROSITEiPS50942. ENTH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Yeast epsin-related proteins required for Golgi-endosome traffic define a gamma-adaptin ear-binding motif."
    Duncan M.C., Costaguta G., Payne G.S.
    Nat. Cell Biol. 5:77-81(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH GGA2, SUBCELLULAR LOCATION, MUTAGENESIS OF PHE-272 AND PHE-275.
  5. "Ent5p is required with Ent3p and Vps27p for ubiquitin-dependent protein sorting into the multivesicular body."
    Eugster A., Pecheur E.-I., Michel F., Winsor B., Letourneur F., Friant S.
    Mol. Biol. Cell 15:3031-3041(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH VPS27.
  6. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  7. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-203, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  8. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-196; SER-198; SER-203; SER-212 AND SER-223, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiENT3_YEAST
AccessioniPrimary (citable) accession number: P47160
Secondary accession number(s): D6VWU4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.