ID   YJ94_YEAST              Reviewed;         448 AA.
AC   P47159; D6VWU3; Q6Q5M7;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   24-JAN-2024, entry version 152.
DE   RecName: Full=Uncharacterized membrane protein YJR124C;
GN   OrderedLocusNames=YJR124C; ORFNames=J2046;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [4]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
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DR   EMBL; Z49624; CAA89655.1; -; Genomic_DNA.
DR   EMBL; AY557924; AAS56250.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08909.1; -; Genomic_DNA.
DR   PIR; S57147; S57147.
DR   RefSeq; NP_012658.3; NM_001181782.3.
DR   AlphaFoldDB; P47159; -.
DR   SMR; P47159; -.
DR   BioGRID; 33880; 51.
DR   DIP; DIP-7198N; -.
DR   MINT; P47159; -.
DR   STRING; 4932.YJR124C; -.
DR   TCDB; 2.A.1.63.4; the major facilitator superfamily (mfs).
DR   iPTMnet; P47159; -.
DR   MaxQB; P47159; -.
DR   PaxDb; 4932-YJR124C; -.
DR   PeptideAtlas; P47159; -.
DR   EnsemblFungi; YJR124C_mRNA; YJR124C; YJR124C.
DR   GeneID; 853588; -.
DR   KEGG; sce:YJR124C; -.
DR   AGR; SGD:S000003885; -.
DR   SGD; S000003885; YJR124C.
DR   VEuPathDB; FungiDB:YJR124C; -.
DR   eggNOG; ENOG502QTZH; Eukaryota.
DR   HOGENOM; CLU_025894_2_0_1; -.
DR   InParanoid; P47159; -.
DR   OMA; LGYGFMP; -.
DR   OrthoDB; 1700720at2759; -.
DR   BioCyc; YEAST:G3O-31745-MONOMER; -.
DR   BioGRID-ORCS; 853588; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P47159; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P47159; Protein.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; HDA:SGD.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   Gene3D; 1.20.1250.20; MFS general substrate transporter like domains; 1.
DR   InterPro; IPR011701; MFS.
DR   InterPro; IPR020846; MFS_dom.
DR   InterPro; IPR036259; MFS_trans_sf.
DR   PANTHER; PTHR23520:SF2; ABR173CP; 1.
DR   PANTHER; PTHR23520; TRANSPORTER, PUTATIVE (AFU_ORTHOLOGUE AFUA_3G04000)-RELATED; 1.
DR   Pfam; PF07690; MFS_1; 2.
DR   SUPFAM; SSF103473; MFS general substrate transporter; 1.
DR   PROSITE; PS50850; MFS; 1.
PE   1: Evidence at protein level;
KW   ATP-binding; Membrane; Nucleotide-binding; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..448
FT                   /note="Uncharacterized membrane protein YJR124C"
FT                   /id="PRO_0000203118"
FT   TOPO_DOM        1..50
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        72..93
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        94..114
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        115..146
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        147..167
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        168..184
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        185..205
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        206..260
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        261..281
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        282..287
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        288..308
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        309..333
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        334..354
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        355..386
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        387..407
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        408..416
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        417..437
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        438..448
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   BINDING         386..393
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        448
FT                   /note="H -> R (in Ref. 3; AAS56250)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   448 AA;  49664 MW;  2319CF9DD1220C14 CRC64;
     MAPEIFVKFK CASRDIKLLW ASVFLRLLSY GLTNQVLTLF LNAINMTEDK IGLFMSLTLA
     GDVICSYILT WYADSWGRRR VLVYGCAMML LSGLVFSFSE NFTLLLVFAI FGVISPSSDE
     VGPFKSIEEA MIAHLSPHNA RPEIYAIHAL VGTIGSALGA IICGIFVDLL KRTGLAATDL
     QCYKLVFLLY AFFAFCKMVI MLLLSDATEL DGHYEHTDCN EETAEPLDVN DETAPLMRQA
     THPEERSNKL SKETVSVLMK LLVIFMVDSL GSGFMTSGWM VYYYSKQFLM GSLALGTLFF
     ITQLVMASST IPSSIIARCF GPVRATLLVQ IPSGIFSILI PMAKNYLPLS ILFLNLHFAT
     TAMDVTPRQI LLTNIIKPRD LTKVMGVVNI GKTFARCVGP IFTGILANNN YLWLCYIISG
     SLVITADLIL ACMFLGVDAK IKKQMNRH
//