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Protein

Putative transferase CAF17, mitochondrial

Gene

IBA57

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for lysine and glutamate prototrophy and mitochondrial genome maintenance. Has a role in the maturation of mitochondrial aconitase-type and radical-SAM Fe/S proteins biotin synthase and lipoic acid synthase.1 Publication

GO - Molecular functioni

  1. transferase activity Source: UniProtKB-KW

GO - Biological processi

  1. iron-sulfur cluster assembly Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciYEAST:G3O-31743-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Putative transferase CAF17, mitochondrial (EC:2.1.-.-)
Alternative name(s):
57 kDa iron-sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins
CCR4-associated factor 17
Gene namesi
Name:IBA57
Synonyms:CAF17
Ordered Locus Names:YJR122W
ORF Names:J2043
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR122w.
SGDiS000003883. IBA57.

Subcellular locationi

Mitochondrion matrix 3 Publications

GO - Cellular componenti

  1. mitochondrial matrix Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 2727MitochondrionSequence AnalysisAdd
BLAST
Chaini28 – 497470Putative transferase CAF17, mitochondrialPRO_0000203117Add
BLAST

Proteomic databases

MaxQBiP47158.
PaxDbiP47158.

Expressioni

Inductioni

Up-regulated during calcium shortage.1 Publication

Gene expression databases

GenevestigatoriP47158.

Interactioni

Subunit structurei

Interacts with CCR4, ISA1 and ISA2.2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
YNL295WP485641EBI-25654,EBI-2046889
YNR075C-AQ8TGJ21EBI-25654,EBI-2047109

Protein-protein interaction databases

BioGridi33878. 12 interactions.
DIPiDIP-1404N.
IntActiP47158. 13 interactions.
MINTiMINT-385256.
STRINGi4932.YJR122W.

Structurei

3D structure databases

ProteinModelPortaliP47158.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GcvT family. CAF17 subfamily.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0354.
GeneTreeiENSGT00390000006465.
HOGENOMiHOG000000825.
InParanoidiP47158.
KOiK06980.
OrthoDBiEOG7P2Z2N.

Family and domain databases

InterProiIPR017703. YgfZ/GcvT_CS.
[Graphical view]
TIGRFAMsiTIGR03317. ygfZ_signature. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47158-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFISRRCRIK GFTLKNLLWF RSSSTRFVST ESPDASAITK PDGIFNYSLL
60 70 80 90 100
ENRTYIRIRG PDTVKFLNGL VTSKLLPHFI KKNLTTVEEN EVPTEEGTTK
110 120 130 140 150
VDPIIPVPEF DARLGNWGLY NEKGIQGPYI SRFGLYSAFL NGKGKLITDT
160 170 180 190 200
IIYPTPVTVS EQISNYPEYL LELHGNVVDK ILHVLQTHKL ANKIKFEKID
210 220 230 240 250
HSSLKTWDVE VQFPNLPKDI ENPWFDNLLD PMALPKNSID ANNFAVNVLN
260 270 280 290 300
SLFNSDPRIL GIYVERRTES MSRHYSTFPQ SFRVVTSEQV DDLSKLFNFN
310 320 330 340 350
VFDFPFQVNK KASVQVREIR FQKGLIDSTE DYISETLLPL ELNFDFFPNT
360 370 380 390 400
ISTNKGCYVG QELTARTYAT GILRKRLVPV KLDNYQLLDT DPERKYAEFH
410 420 430 440 450
IDNVVEKSLA ENEPTLNPFT NKPPERTKRK QRPAGLLISN EGLYGVALLR
460 470 480 490
TEHFSAAFSS DEPVEFYITT TKGENIKITP QKPFWFSDWK NNNGPHK
Length:497
Mass (Da):57,055
Last modified:January 31, 1996 - v1
Checksum:iA9970EBD3BF6019C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49622 Genomic DNA. Translation: CAA89653.1.
BK006943 Genomic DNA. Translation: DAA08907.1.
PIRiS57145.
RefSeqiNP_012656.1. NM_001181780.1.

Genome annotation databases

EnsemblFungiiYJR122W; YJR122W; YJR122W.
GeneIDi853586.
KEGGisce:YJR122W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49622 Genomic DNA. Translation: CAA89653.1.
BK006943 Genomic DNA. Translation: DAA08907.1.
PIRiS57145.
RefSeqiNP_012656.1. NM_001181780.1.

3D structure databases

ProteinModelPortaliP47158.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33878. 12 interactions.
DIPiDIP-1404N.
IntActiP47158. 13 interactions.
MINTiMINT-385256.
STRINGi4932.YJR122W.

Proteomic databases

MaxQBiP47158.
PaxDbiP47158.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR122W; YJR122W; YJR122W.
GeneIDi853586.
KEGGisce:YJR122W.

Organism-specific databases

CYGDiYJR122w.
SGDiS000003883. IBA57.

Phylogenomic databases

eggNOGiCOG0354.
GeneTreeiENSGT00390000006465.
HOGENOMiHOG000000825.
InParanoidiP47158.
KOiK06980.
OrthoDBiEOG7P2Z2N.

Enzyme and pathway databases

BioCyciYEAST:G3O-31743-MONOMER.

Miscellaneous databases

NextBioi974385.

Gene expression databases

GenevestigatoriP47158.

Family and domain databases

InterProiIPR017703. YgfZ/GcvT_CS.
[Graphical view]
TIGRFAMsiTIGR03317. ygfZ_signature. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "Genome-wide analysis of yeast transcription upon calcium shortage."
    Lombardia L.J., Becerra M., Rodriguez-Belmonte E., Hauser N.C., Cerdan M.E.
    Cell Calcium 32:83-91(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: INDUCTION.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Systematic mutagenesis of the leucine-rich repeat (LRR) domain of CCR4 reveals specific sites for binding to CAF1 and a separate critical role for the LRR in CCR4 deadenylase activity."
    Clark L.B., Viswanathan P., Quigley G., Chiang Y.-C., McMahon J.S., Yao G., Chen J., Nelsbach A., Denis C.L.
    J. Biol. Chem. 279:13616-13623(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CCR4.
  7. "Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
    Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
    J. Proteome Res. 5:1543-1554(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Mitochondrial Iba57p is required for Fe/S cluster formation on aconitase and activation of radical SAM enzymes."
    Gelling C., Dawes I.W., Richhardt N., Lill R., Muehlenhoff U.
    Mol. Cell. Biol. 28:1851-1861(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ISA1 AND ISA2.

Entry informationi

Entry nameiCAF17_YEAST
AccessioniPrimary (citable) accession number: P47158
Secondary accession number(s): D6VWU1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 31, 1996
Last sequence update: January 31, 1996
Last modified: January 6, 2015
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2960 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.