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P47158 (CAF17_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 96. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Putative transferase CAF17, mitochondrial

EC=2.1.-.-
Alternative name(s):
57 kDa iron-sulfur cluster assembly factor for biotin synthase- and aconitase-like mitochondrial proteins
CCR4-associated factor 17
Gene names
Name:IBA57
Synonyms:CAF17
Ordered Locus Names:YJR122W
ORF Names:J2043
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for lysine and glutamate prototrophy and mitochondrial genome maintenance. Has a role in the maturation of mitochondrial aconitase-type and radical-SAM Fe/S proteins biotin synthase and lipoic acid synthase. Ref.8

Subunit structure

Interacts with CCR4, ISA1 and ISA2. Ref.6 Ref.8

Subcellular location

Mitochondrion matrix Ref.4 Ref.7 Ref.8.

Induction

Up-regulated during calcium shortage. Ref.3

Miscellaneous

Present with 2960 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GcvT family. CAF17 subfamily.

Ontologies

Keywords
   Cellular componentMitochondrion
   DomainTransit peptide
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processiron-sulfur cluster assembly

Inferred from mutant phenotype Ref.8. Source: SGD

   Cellular_componentmitochondrial matrix

Inferred from direct assay Ref.8. Source: SGD

   Molecular_functiontransferase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

YNL295WP485641EBI-25654,EBI-2046889
YNR075C-AQ8TGJ21EBI-25654,EBI-2047109

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 2727Mitochondrion Potential
Chain28 – 497470Putative transferase CAF17, mitochondrial
PRO_0000203117

Sequences

Sequence LengthMass (Da)Tools
P47158 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: A9970EBD3BF6019C

FASTA49757,055
        10         20         30         40         50         60 
MFISRRCRIK GFTLKNLLWF RSSSTRFVST ESPDASAITK PDGIFNYSLL ENRTYIRIRG 

        70         80         90        100        110        120 
PDTVKFLNGL VTSKLLPHFI KKNLTTVEEN EVPTEEGTTK VDPIIPVPEF DARLGNWGLY 

       130        140        150        160        170        180 
NEKGIQGPYI SRFGLYSAFL NGKGKLITDT IIYPTPVTVS EQISNYPEYL LELHGNVVDK 

       190        200        210        220        230        240 
ILHVLQTHKL ANKIKFEKID HSSLKTWDVE VQFPNLPKDI ENPWFDNLLD PMALPKNSID 

       250        260        270        280        290        300 
ANNFAVNVLN SLFNSDPRIL GIYVERRTES MSRHYSTFPQ SFRVVTSEQV DDLSKLFNFN 

       310        320        330        340        350        360 
VFDFPFQVNK KASVQVREIR FQKGLIDSTE DYISETLLPL ELNFDFFPNT ISTNKGCYVG 

       370        380        390        400        410        420 
QELTARTYAT GILRKRLVPV KLDNYQLLDT DPERKYAEFH IDNVVEKSLA ENEPTLNPFT 

       430        440        450        460        470        480 
NKPPERTKRK QRPAGLLISN EGLYGVALLR TEHFSAAFSS DEPVEFYITT TKGENIKITP 

       490 
QKPFWFSDWK NNNGPHK 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Genome-wide analysis of yeast transcription upon calcium shortage."
Lombardia L.J., Becerra M., Rodriguez-Belmonte E., Hauser N.C., Cerdan M.E.
Cell Calcium 32:83-91(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INDUCTION.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Systematic mutagenesis of the leucine-rich repeat (LRR) domain of CCR4 reveals specific sites for binding to CAF1 and a separate critical role for the LRR in CCR4 deadenylase activity."
Clark L.B., Viswanathan P., Quigley G., Chiang Y.-C., McMahon J.S., Yao G., Chen J., Nelsbach A., Denis C.L.
J. Biol. Chem. 279:13616-13623(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CCR4.
[7]"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics."
Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A.
J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Mitochondrial Iba57p is required for Fe/S cluster formation on aconitase and activation of radical SAM enzymes."
Gelling C., Dawes I.W., Richhardt N., Lill R., Muehlenhoff U.
Mol. Cell. Biol. 28:1851-1861(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH ISA1 AND ISA2.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49622 Genomic DNA. Translation: CAA89653.1.
BK006943 Genomic DNA. Translation: DAA08907.1.
PIRS57145.
RefSeqNP_012656.1. NM_001181780.1.

3D structure databases

ProteinModelPortalP47158.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33878. 12 interactions.
DIPDIP-1404N.
IntActP47158. 13 interactions.
MINTMINT-385256.
STRING4932.YJR122W.

Proteomic databases

PaxDbP47158.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR122W; YJR122W; YJR122W.
GeneID853586.
KEGGsce:YJR122W.

Organism-specific databases

CYGDYJR122w.
SGDS000003883. IBA57.

Phylogenomic databases

eggNOGCOG0354.
GeneTreeENSGT00390000006465.
HOGENOMHOG000000825.
KOK06980.
OrthoDBEOG7P2Z2N.

Enzyme and pathway databases

BioCycYEAST:G3O-31743-MONOMER.

Gene expression databases

GenevestigatorP47158.

Family and domain databases

InterProIPR017703. YgfZ/GcvT_CS.
[Graphical view]
TIGRFAMsTIGR03317. ygfZ_signature. 1 hit.
ProtoNetSearch...

Other

NextBio974385.

Entry information

Entry nameCAF17_YEAST
AccessionPrimary (citable) accession number: P47158
Secondary accession number(s): D6VWU1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 19, 2014
This is version 96 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families