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P47153 (TDA4_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 87. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Topoisomerase I damage affected protein 4
Gene names
Name:TDA4
Ordered Locus Names:YJR116W
ORF Names:J2031
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length279 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Subcellular location

Membrane; Multi-pass membrane protein.

Disruption phenotype

Leads to cell death when overexpressing the camptothecin mimetic TOP1-T(722)A mutant. Ref.4

Sequence similarities

Belongs to the TMEM56 family.

Contains 1 TLC (TRAM/LAG1/CLN8) domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainTransmembrane
Transmembrane helix
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentintegral to membrane

Inferred from sequence model PubMed 12192589. Source: SGD

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 279279Topoisomerase I damage affected protein 4
PRO_0000185544

Regions

Topological domain1 – 3232Extracellular Potential
Transmembrane33 – 5321Helical; Potential
Topological domain54 – 7926Cytoplasmic Potential
Transmembrane80 – 10021Helical; Potential
Topological domain101 – 11010Extracellular Potential
Transmembrane111 – 13121Helical; Potential
Topological domain132 – 1354Cytoplasmic Potential
Transmembrane136 – 15621Helical; Potential
Topological domain157 – 1626Extracellular Potential
Transmembrane163 – 18321Helical; Potential
Topological domain184 – 1929Cytoplasmic Potential
Transmembrane193 – 21321Helical; Potential
Topological domain214 – 23825Extracellular Potential
Transmembrane239 – 25921Helical; Potential
Topological domain260 – 27920Cytoplasmic Potential
Domain70 – 271202TLC

Sequences

Sequence LengthMass (Da)Tools
P47153 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 6610293B38B7F2B5

FASTA27932,011
        10         20         30         40         50         60 
MNANSTTTAI GLTSPFEKLS FFPHSSNLIL AHLHEIIFSF VFYQLAFSVV APFLNKVVFR 

        70         80         90        100        110        120 
KHYTTIRDPL LKIDFNVHTV SMIQAVVSNT VLLPTLTTPM HYNVVTYTDS YSSMVSSLSA 

       130        140        150        160        170        180 
GYFIWDLTMC VRYFKLYGLE FTGHAIGSVY VMLLSLRPFC QPWIGRFLIY EASTPFVNIN 

       190        200        210        220        230        240 
WFIMQCNAKS KNSIPLWFNV VNGLLLMTVF FVVRICWGSI ASALLFRQMW KVRDELPKFS 

       250        260        270 
AVTMMSLNIF MNLLNVLWFK KMIRIAKKLA KPAPTSKLD

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"A global topology map of the Saccharomyces cerevisiae membrane proteome."
Kim H., Melen K., Oesterberg M., von Heijne G.
Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: ATCC 208353 / W303-1A.
[4]"Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage."
Reid R.J., Gonzalez-Barrera S., Sunjevaric I., Alvaro D., Ciccone S., Wagner M., Rothstein R.
Genome Res. 21:477-486(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		Z49616 Genomic DNA. Translation: CAA89646.1.
BK006943 Genomic DNA. Translation: DAA08901.1.
PIRS57139.
RefSeqNP_012650.1. NM_001181774.1.

3D structure databases

ProteinModelPortalP47153.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4077N.
MINTMINT-554519.
STRING4932.YJR116W.

Proteomic databases

PaxDbP47153.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR116W; YJR116W; YJR116W.
GeneID853580.
KEGGsce:YJR116W.

Organism-specific databases

CYGDYJR116w.
SGDS000003877. TDA4.

Phylogenomic databases

eggNOGNOG327998.
GeneTreeENSGT00530000068374.
OMAPFVNINW.
OrthoDBEOG4GMZ67.

Gene expression databases

GenevestigatorP47153.
GermOnlineYJR116W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR006634. TLC-dom.
[Graphical view]
PfamPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
SMARTSM00724. TLC. 1 hit.
[Graphical view]
PROSITEPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974367.

Entry information

Entry nameTDA4_YEAST
AccessionPrimary (citable) accession number: P47153
Secondary accession number(s): D6VWT5
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 3, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

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