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Protein

Topoisomerase I damage affected protein 4

Gene

TDA4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Enzyme and pathway databases

BioCyciYEAST:G3O-31738-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Topoisomerase I damage affected protein 4
Gene namesi
Name:TDA4
Ordered Locus Names:YJR116W
ORF Names:J2031
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR116w.
EuPathDBiFungiDB:YJR116W.
SGDiS000003877. TDA4.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3232ExtracellularSequence AnalysisAdd
BLAST
Transmembranei33 – 5321HelicalSequence AnalysisAdd
BLAST
Topological domaini54 – 7926CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei80 – 10021HelicalSequence AnalysisAdd
BLAST
Topological domaini101 – 11010ExtracellularSequence Analysis
Transmembranei111 – 13121HelicalSequence AnalysisAdd
BLAST
Topological domaini132 – 1354CytoplasmicSequence Analysis
Transmembranei136 – 15621HelicalSequence AnalysisAdd
BLAST
Topological domaini157 – 1626ExtracellularSequence Analysis
Transmembranei163 – 18321HelicalSequence AnalysisAdd
BLAST
Topological domaini184 – 1929CytoplasmicSequence Analysis
Transmembranei193 – 21321HelicalSequence AnalysisAdd
BLAST
Topological domaini214 – 23825ExtracellularSequence AnalysisAdd
BLAST
Transmembranei239 – 25921HelicalSequence AnalysisAdd
BLAST
Topological domaini260 – 27920CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Disruption phenotypei

Leads to cell death when overexpressing the camptothecin mimetic TOP1-T(722)A mutant.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 279279Topoisomerase I damage affected protein 4PRO_0000185544Add
BLAST

Proteomic databases

PaxDbiP47153.

Interactioni

Protein-protein interaction databases

BioGridi33872. 7 interactions.
DIPiDIP-4077N.
MINTiMINT-554519.

Structurei

3D structure databases

ProteinModelPortaliP47153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini70 – 271202TLCPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the TMEM56 family.Curated
Contains 1 TLC (TRAM/LAG1/CLN8) domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG327998.
GeneTreeiENSGT00530000068374.
InParanoidiP47153.
OMAiYGGIMSY.
OrthoDBiEOG74J9MF.

Family and domain databases

InterProiIPR006634. TLC-dom.
[Graphical view]
PfamiPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47153-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNANSTTTAI GLTSPFEKLS FFPHSSNLIL AHLHEIIFSF VFYQLAFSVV
60 70 80 90 100
APFLNKVVFR KHYTTIRDPL LKIDFNVHTV SMIQAVVSNT VLLPTLTTPM
110 120 130 140 150
HYNVVTYTDS YSSMVSSLSA GYFIWDLTMC VRYFKLYGLE FTGHAIGSVY
160 170 180 190 200
VMLLSLRPFC QPWIGRFLIY EASTPFVNIN WFIMQCNAKS KNSIPLWFNV
210 220 230 240 250
VNGLLLMTVF FVVRICWGSI ASALLFRQMW KVRDELPKFS AVTMMSLNIF
260 270
MNLLNVLWFK KMIRIAKKLA KPAPTSKLD
Length:279
Mass (Da):32,011
Last modified:February 1, 1996 - v1
Checksum:i6610293B38B7F2B5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49616 Genomic DNA. Translation: CAA89646.1.
BK006943 Genomic DNA. Translation: DAA08901.1.
PIRiS57139.
RefSeqiNP_012650.1. NM_001181774.1.

Genome annotation databases

EnsemblFungiiYJR116W; YJR116W; YJR116W.
GeneIDi853580.
KEGGisce:YJR116W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49616 Genomic DNA. Translation: CAA89646.1.
BK006943 Genomic DNA. Translation: DAA08901.1.
PIRiS57139.
RefSeqiNP_012650.1. NM_001181774.1.

3D structure databases

ProteinModelPortaliP47153.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33872. 7 interactions.
DIPiDIP-4077N.
MINTiMINT-554519.

Proteomic databases

PaxDbiP47153.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR116W; YJR116W; YJR116W.
GeneIDi853580.
KEGGisce:YJR116W.

Organism-specific databases

CYGDiYJR116w.
EuPathDBiFungiDB:YJR116W.
SGDiS000003877. TDA4.

Phylogenomic databases

eggNOGiNOG327998.
GeneTreeiENSGT00530000068374.
InParanoidiP47153.
OMAiYGGIMSY.
OrthoDBiEOG74J9MF.

Enzyme and pathway databases

BioCyciYEAST:G3O-31738-MONOMER.

Miscellaneous databases

NextBioi974367.
PROiP47153.

Family and domain databases

InterProiIPR006634. TLC-dom.
[Graphical view]
PfamiPF03798. TRAM_LAG1_CLN8. 1 hit.
[Graphical view]
SMARTiSM00724. TLC. 1 hit.
[Graphical view]
PROSITEiPS50922. TLC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  4. "Selective ploidy ablation, a high-throughput plasmid transfer protocol, identifies new genes affecting topoisomerase I-induced DNA damage."
    Reid R.J., Gonzalez-Barrera S., Sunjevaric I., Alvaro D., Ciccone S., Wagner M., Rothstein R.
    Genome Res. 21:477-486(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiTDA4_YEAST
AccessioniPrimary (citable) accession number: P47153
Secondary accession number(s): D6VWT5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 22, 2015
This is version 101 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.