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Protein

Kinetochore-associated protein NNF1

Gene

NNF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Acts as essential component of the kinetochore MIND complex, which is required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules. NNF1 is required for a number of nuclear functions. Cells depleted of NNF11 or containing a temperature-sensitive NNF1 mutation have elongated microtubules and become bi- and multinucleate. They also have a fragmented nucleolus and accumulate poly(A)+ RNA inside the nucleus.2 Publications

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31735-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore-associated protein NNF1
Gene namesi
Name:NNF1
Ordered Locus Names:YJR112W
ORF Names:J2011
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR112W.
SGDiS000003873. NNF1.

Subcellular locationi

GO - Cellular componenti

  • kinetochore Source: SGD
  • nuclear MIS12/MIND complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968851 – 201Kinetochore-associated protein NNF1Add BLAST201

Proteomic databases

MaxQBiP47149.
PRIDEiP47149.

Interactioni

Subunit structurei

Component of the MIND kinetochore complex, which is composed of at least MTW1, NNF1, NSL1 and DSN1.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
MTW1P397316EBI-12098,EBI-11606
PAC1P399463EBI-12098,EBI-12838
SMC1P329086EBI-12098,EBI-17402

Protein-protein interaction databases

BioGridi33868. 109 interactors.
DIPiDIP-3017N.
IntActiP47149. 23 interactors.
MINTiMINT-434685.

Structurei

3D structure databases

ProteinModelPortaliP47149.
SMRiP47149.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000248864.
InParanoidiP47149.
KOiK11562.
OMAiISKLQNW.
OrthoDBiEOG092C5A3C.

Family and domain databases

InterProiIPR016851. Kinetochore-assoc_NNF1.
IPR007128. PMF1/NNF1.
[Graphical view]
PfamiPF03980. Nnf1. 1 hit.
[Graphical view]
PIRSFiPIRSF027153. Nnf1p. 1 hit.

Sequencei

Sequence statusi: Complete.

P47149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNSHGIRYI RLKQVFNRAL DQSISKLQSW DKVSSCFPQY VNSKQGAINV
60 70 80 90 100
ANCQRQLTEF WTELCQREFK EIMEERNVEQ KLNELDELIL EAKERYTDRD
110 120 130 140 150
QDEVNKGPAI DELSSKELVE CHLYSQRMHA IHEIDERLAK VNEMNDQLAQ
160 170 180 190 200
ELKDLETQVE VEKNEIGKMY DEYLGSHTDQ PANVLLVQSL NDMVLELKEN

Y
Length:201
Mass (Da):23,640
Last modified:September 27, 2004 - v3
Checksum:i53568A8E6EA95694
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55R → S in CAA63725 (PubMed:9247195).Curated1
Sequence conflicti89I → M in CAA63725 (PubMed:9247195).Curated1
Sequence conflicti152L → F in CAA63725 (PubMed:9247195).Curated1
Sequence conflicti164 – 165NE → KQ in CAA63725 (PubMed:9247195).Curated2
Sequence conflicti193M → K in CAA63725 (PubMed:9247195).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93350 Genomic DNA. Translation: CAA63725.1.
Z49612 Genomic DNA. Translation: CAA89642.1.
AY558256 Genomic DNA. Translation: AAS56582.1.
BK006943 Genomic DNA. Translation: DAA08897.1.
PIRiS57135.
RefSeqiNP_012646.3. NM_001181770.3.

Genome annotation databases

EnsemblFungiiYJR112W; YJR112W; YJR112W.
GeneIDi853576.
KEGGisce:YJR112W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93350 Genomic DNA. Translation: CAA63725.1.
Z49612 Genomic DNA. Translation: CAA89642.1.
AY558256 Genomic DNA. Translation: AAS56582.1.
BK006943 Genomic DNA. Translation: DAA08897.1.
PIRiS57135.
RefSeqiNP_012646.3. NM_001181770.3.

3D structure databases

ProteinModelPortaliP47149.
SMRiP47149.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33868. 109 interactors.
DIPiDIP-3017N.
IntActiP47149. 23 interactors.
MINTiMINT-434685.

Proteomic databases

MaxQBiP47149.
PRIDEiP47149.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR112W; YJR112W; YJR112W.
GeneIDi853576.
KEGGisce:YJR112W.

Organism-specific databases

EuPathDBiFungiDB:YJR112W.
SGDiS000003873. NNF1.

Phylogenomic databases

HOGENOMiHOG000248864.
InParanoidiP47149.
KOiK11562.
OMAiISKLQNW.
OrthoDBiEOG092C5A3C.

Enzyme and pathway databases

BioCyciYEAST:G3O-31735-MONOMER.

Miscellaneous databases

PROiP47149.

Family and domain databases

InterProiIPR016851. Kinetochore-assoc_NNF1.
IPR007128. PMF1/NNF1.
[Graphical view]
PfamiPF03980. Nnf1. 1 hit.
[Graphical view]
PIRSFiPIRSF027153. Nnf1p. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiNNF1_YEAST
AccessioniPrimary (citable) accession number: P47149
Secondary accession number(s): D6VWT1, Q06779
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 27, 2004
Last modified: November 2, 2016
This is version 126 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.