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Protein

Kinetochore-associated protein NNF1

Gene

NNF1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts as essential component of the kinetochore MIND complex, which is required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules. NNF1 is required for a number of nuclear functions. Cells depleted of NNF11 or containing a temperature-sensitive NNF1 mutation have elongated microtubules and become bi- and multinucleate. They also have a fragmented nucleolus and accumulate poly(A)+ RNA inside the nucleus.2 Publications

Miscellaneous

Present with 2070 molecules/cell in log phase SD medium.1 Publication

GO - Biological processi

  • cell cycle Source: UniProtKB-KW
  • cell division Source: UniProtKB-KW
  • chromosome segregation Source: SGD

Keywordsi

Biological processCell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31735-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Kinetochore-associated protein NNF1
Gene namesi
Name:NNF1
Ordered Locus Names:YJR112W
ORF Names:J2011
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR112W
SGDiS000003873 NNF1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000968851 – 201Kinetochore-associated protein NNF1Add BLAST201

Proteomic databases

MaxQBiP47149
PaxDbiP47149
PRIDEiP47149

Interactioni

Subunit structurei

Component of the MIND kinetochore complex, which is composed of at least MTW1, NNF1, NSL1 and DSN1.4 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi33868, 250 interactors
DIPiDIP-3017N
IntActiP47149, 28 interactors
MINTiP47149
STRINGi4932.YJR112W

Structurei

3D structure databases

ProteinModelPortaliP47149
SMRiP47149
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

HOGENOMiHOG000248864
InParanoidiP47149
KOiK11562
OMAiFWHANSL
OrthoDBiEOG092C5A3C

Family and domain databases

InterProiView protein in InterPro
IPR016851 Kinetochore-assoc_NNF1
IPR007128 PMF1/NNF1
PfamiView protein in Pfam
PF03980 Nnf1, 1 hit
PIRSFiPIRSF027153 Nnf1p, 1 hit

Sequencei

Sequence statusi: Complete.

P47149-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVNSHGIRYI RLKQVFNRAL DQSISKLQSW DKVSSCFPQY VNSKQGAINV
60 70 80 90 100
ANCQRQLTEF WTELCQREFK EIMEERNVEQ KLNELDELIL EAKERYTDRD
110 120 130 140 150
QDEVNKGPAI DELSSKELVE CHLYSQRMHA IHEIDERLAK VNEMNDQLAQ
160 170 180 190 200
ELKDLETQVE VEKNEIGKMY DEYLGSHTDQ PANVLLVQSL NDMVLELKEN

Y
Length:201
Mass (Da):23,640
Last modified:September 27, 2004 - v3
Checksum:i53568A8E6EA95694
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti55R → S in CAA63725 (PubMed:9247195).Curated1
Sequence conflicti89I → M in CAA63725 (PubMed:9247195).Curated1
Sequence conflicti152L → F in CAA63725 (PubMed:9247195).Curated1
Sequence conflicti164 – 165NE → KQ in CAA63725 (PubMed:9247195).Curated2
Sequence conflicti193M → K in CAA63725 (PubMed:9247195).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X93350 Genomic DNA Translation: CAA63725.1
Z49612 Genomic DNA Translation: CAA89642.1
AY558256 Genomic DNA Translation: AAS56582.1
BK006943 Genomic DNA Translation: DAA08897.1
PIRiS57135
RefSeqiNP_012646.3, NM_001181770.3

Genome annotation databases

EnsemblFungiiYJR112W; YJR112W; YJR112W
GeneIDi853576
KEGGisce:YJR112W

Similar proteinsi

Entry informationi

Entry nameiNNF1_YEAST
AccessioniPrimary (citable) accession number: P47149
Secondary accession number(s): D6VWT1, Q06779
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: September 27, 2004
Last modified: March 28, 2018
This is version 137 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health