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Protein

Adenosine kinase

Gene

ADO1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. ADO1 does not play a major role in adenine utilization in yeast. Its physiological role could primarily be to recycle adenosine produced by the methyl cycle.

Catalytic activityi

ATP + adenosine = ADP + AMP.

Cofactori

Mg2+By similarity

Pathwayi: AMP biosynthesis via salvage pathway

This protein is involved in step 1 of the subpathway that synthesizes AMP from adenosine.
Proteins known to be involved in this subpathway in this organism are:
  1. Adenosine kinase (ADO1)
This subpathway is part of the pathway AMP biosynthesis via salvage pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes AMP from adenosine, the pathway AMP biosynthesis via salvage pathway and in Purine metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei293 – 2931By similarity

GO - Molecular functioni

  • adenosine kinase activity Source: SGD
  • ATP binding Source: UniProtKB-KW

GO - Biological processi

  • AMP salvage Source: UniProtKB-UniPathway
  • purine nucleobase metabolic process Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Purine salvage

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:YJR105W-MONOMER.
ReactomeiR-SCE-74217. Purine salvage.
UniPathwayiUPA00588; UER00659.

Names & Taxonomyi

Protein namesi
Recommended name:
Adenosine kinase (EC:2.7.1.20)
Gene namesi
Name:ADO1
Ordered Locus Names:YJR105W
ORF Names:J1973
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR105W.
SGDiS000003866. ADO1.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Adenosine kinasePRO_0000080062Add
BLAST

Proteomic databases

MaxQBiP47143.

Interactioni

Protein-protein interaction databases

BioGridi33861. 167 interactions.
DIPiDIP-4681N.
IntActiP47143. 1 interaction.
MINTiMINT-542654.

Structurei

3D structure databases

ProteinModelPortaliP47143.
SMRiP47143. Positions 8-321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the carbohydrate kinase PfkB family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014320.
HOGENOMiHOG000172419.
InParanoidiP47143.
KOiK00856.
OMAiISQFYMA.
OrthoDBiEOG79W9FW.

Family and domain databases

Gene3Di3.40.1190.20. 2 hits.
InterProiIPR001805. Adenokinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10584:SF24. PTHR10584:SF24. 1 hit.
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00989. ADENOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47143-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTAPLVVLGN PLLDFQADVT AEYLAKYSLK ENDAILVDAK SGDAKMAIFD
60 70 80 90 100
ELLQMPETKL VAGGAAQNTA RGAAYVLGAG QVVYFGSVGK DKFSERLLNE
110 120 130 140 150
NEKAGVKSMY QVQNDIGTGK CAALITGHNR SLVTDLGAAN FFTPDHLDKH
160 170 180 190 200
WDLVEAAKLF YIGGFHLTVS PDAIVKLGQH AKENSKPFVL NFSAPFIPHV
210 220 230 240 250
FKDALARVLP YATVIIANES EAEAFCDAFQ LDCANTDLEA IAQRIVKDSP
260 270 280 290 300
VEKTVIFTHG VEPTVVVSSK GTSTYPVKPL DSSKIVDTNG AGDAFAGGFM
310 320 330 340
AGLTKGEDLE TSIDMGQWLA ALSIQEVGPS YPSEKISYSK
Length:340
Mass (Da):36,372
Last modified:February 1, 1996 - v1
Checksum:iF0F18B5831F025A5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49605 Genomic DNA. Translation: CAA89635.1.
AY558082 Genomic DNA. Translation: AAS56408.1.
BK006943 Genomic DNA. Translation: DAA08890.1.
PIRiS57126.
RefSeqiNP_012639.1. NM_001181763.1.

Genome annotation databases

EnsemblFungiiYJR105W; YJR105W; YJR105W.
GeneIDi853569.
KEGGisce:YJR105W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49605 Genomic DNA. Translation: CAA89635.1.
AY558082 Genomic DNA. Translation: AAS56408.1.
BK006943 Genomic DNA. Translation: DAA08890.1.
PIRiS57126.
RefSeqiNP_012639.1. NM_001181763.1.

3D structure databases

ProteinModelPortaliP47143.
SMRiP47143. Positions 8-321.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33861. 167 interactions.
DIPiDIP-4681N.
IntActiP47143. 1 interaction.
MINTiMINT-542654.

Proteomic databases

MaxQBiP47143.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR105W; YJR105W; YJR105W.
GeneIDi853569.
KEGGisce:YJR105W.

Organism-specific databases

EuPathDBiFungiDB:YJR105W.
SGDiS000003866. ADO1.

Phylogenomic databases

GeneTreeiENSGT00390000014320.
HOGENOMiHOG000172419.
InParanoidiP47143.
KOiK00856.
OMAiISQFYMA.
OrthoDBiEOG79W9FW.

Enzyme and pathway databases

UniPathwayiUPA00588; UER00659.
BioCyciYEAST:YJR105W-MONOMER.
ReactomeiR-SCE-74217. Purine salvage.

Miscellaneous databases

PROiP47143.

Family and domain databases

Gene3Di3.40.1190.20. 2 hits.
InterProiIPR001805. Adenokinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
IPR011611. PfkB_dom.
IPR029056. Ribokinase-like.
[Graphical view]
PANTHERiPTHR10584:SF24. PTHR10584:SF24. 1 hit.
PfamiPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSiPR00989. ADENOKINASE.
SUPFAMiSSF53613. SSF53613. 1 hit.
PROSITEiPS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "Role of adenosine kinase in Saccharomyces cerevisiae: identification of the ADO1 gene and study of the mutant phenotypes."
    Lecoq K., Belloc I., Desgranges C., Daignan-Fornier B.
    Yeast 18:335-342(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Expression in Escherichia coli of a recombinant adenosine kinase from Saccharomyces cerevisiae: purification, kinetics and substrate analyses."
    Barrado P., Rodriguez M.J., Jimenez A., Lobato M.F.
    Yeast 20:1145-1150(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.

Entry informationi

Entry nameiADK_YEAST
AccessioniPrimary (citable) accession number: P47143
Secondary accession number(s): D6VWS4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: July 6, 2016
This is version 146 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 22200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.