Reviewed, UniProtKB/Swiss-Prot P47143 (ADK_YEAST)

Names and origin

Protein names

Recommended name:
    Adenosine kinase
    EC=2.7.1.20

Gene names

Name:	ADO1
Ordered Locus Names:	YJR105W
ORF Names:	J1973

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. ADO1 does not play a major role in adenine utilization in yeast. Its physiological role could primarily be to recycle adenosine produced by the methyl cycle.
Catalytic activity	ATP + adenosine = ADP + AMP.
Cofactor	Magnesium By similarity.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine 3',5'-bisphosphate: step 1/1.
Miscellaneous	Present with 22200 molecules/cell in log phase SD medium. Ref.4
Sequence similarities	Belongs to the carbohydrate kinase pfkB family.

Ontologies

Keywords
Biological process	Purine salvage
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base metabolic process Ref.3 Inferred from mutant phenotype. Source: SGD purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW adenosine kinase activity Ref.3 Inferred from direct assay. Source: SGD identical protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
itself		1	EBI-2243,EBI-2243

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Adenosine kinase		PRO_0000080062
Sites
	Active site	293	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47143-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: F0F18B5831F025A5	FASTA	340	36,372
	10 20 30 40 50 60 MTAPLVVLGN PLLDFQADVT AEYLAKYSLK ENDAILVDAK SGDAKMAIFD ELLQMPETKL 70 80 90 100 110 120 VAGGAAQNTA RGAAYVLGAG QVVYFGSVGK DKFSERLLNE NEKAGVKSMY QVQNDIGTGK 130 140 150 160 170 180 CAALITGHNR SLVTDLGAAN FFTPDHLDKH WDLVEAAKLF YIGGFHLTVS PDAIVKLGQH 190 200 210 220 230 240 AKENSKPFVL NFSAPFIPHV FKDALARVLP YATVIIANES EAEAFCDAFQ LDCANTDLEA 250 260 270 280 290 300 IAQRIVKDSP VEKTVIFTHG VEPTVVVSSK GTSTYPVKPL DSSKIVDTNG AGDAFAGGFM 310 320 330 340 AGLTKGEDLE TSIDMGQWLA ALSIQEVGPS YPSEKISYSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[2]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Role of adenosine kinase in Saccharomyces cerevisiae: identification of the ADO1 gene and study of the mutant phenotypes." Lecoq K., Belloc I., Desgranges C., Daignan-Fornier B. Yeast 18:335-342(2001) [PubMed: 11223943] [Abstract] Cited for: CHARACTERIZATION.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Expression in Escherichia coli of a recombinant adenosine kinase from Saccharomyces cerevisiae: purification, kinetics and substrate analyses." Barrado P., Rodriguez M.J., Jimenez A., Lobato M.F. Yeast 20:1145-1150(2003) [PubMed: 14558146] [Abstract] Cited for: CHARACTERIZATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49605 Genomic DNA. Translation: CAA89635.1. AY558082 Genomic DNA. Translation: AAS56408.1.
PIR	S57126.
RefSeq	NP_012639.1.
3D structure databases
SMR	P47143. Positions 1-334.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-4681N.
IntAct	P47143. 6 interactions.
STRING	P47143.
Proteomic databases
PeptideAtlas	P47143.
Genome annotation databases
Ensembl	YJR105W; YJR105W; YJR105W; Saccharomyces cerevisiae. [Genome view]
GeneID	853569.
KEGG	sce:YJR105W.
NMPDR	fig|4932.3.peg.3613.
Organism-specific databases
CYGD	YJR105w.
SGD	S000003866. ADO1.
Phylogenomic databases
eggNOG	fuNOG07084.
HOGENOM	HBG314716.
OMA	AQWMIQQ.
OrthoDB	EOG9WQ2K9.
PhylomeDB	P47143.
Enzyme and pathway databases
BRENDA	2.7.1.20. 250.
Gene expression databases
ArrayExpress	P47143.
Genevestigator	P47143.
GermOnline	YJR105W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR001805. Adenokinase. IPR011611. Carb/pur_kinase. IPR002173. Carboh/pur_kinase_PfkB_CS. [Graphical view]
PANTHER	PTHR10584:SF24. Adenokinase. 1 hit.
Pfam	PF00294. PfkB. 1 hit. [Graphical view]
PRINTS	PR00989. ADENOKINASE.
PROSITE	PS00583. PFKB_KINASES_1. False negative. PS00584. PFKB_KINASES_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974337.

Entry information

Entry name

ADK_YEAST

Accession

Primary (citable) accession number: P47143

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	January 19, 2010
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)