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P47143 (ADK_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 106. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Adenosine kinase

EC=2.7.1.20
Gene names
Name:ADO1
Ordered Locus Names:YJR105W
ORF Names:J1973
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length340 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATP dependent phosphorylation of adenosine and other related nucleoside analogs to monophosphate derivatives. ADO1 does not play a major role in adenine utilization in yeast. Its physiological role could primarily be to recycle adenosine produced by the methyl cycle.

Catalytic activity

ATP + adenosine = ADP + AMP.

Cofactor

Magnesium By similarity.

Pathway

Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenosine: step 1/1.

Miscellaneous

Present with 22200 molecules/cell in log phase SD medium. Ref.5

Sequence similarities

Belongs to the carbohydrate kinase pfkB family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

itself1EBI-2243,EBI-2243

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 340340Adenosine kinase
PRO_0000080062

Sites

Active site2931 By similarity

Sequences

Sequence LengthMass (Da)Tools
P47143 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: F0F18B5831F025A5

FASTA34036,372
        10         20         30         40         50         60 
MTAPLVVLGN PLLDFQADVT AEYLAKYSLK ENDAILVDAK SGDAKMAIFD ELLQMPETKL 

        70         80         90        100        110        120 
VAGGAAQNTA RGAAYVLGAG QVVYFGSVGK DKFSERLLNE NEKAGVKSMY QVQNDIGTGK 

       130        140        150        160        170        180 
CAALITGHNR SLVTDLGAAN FFTPDHLDKH WDLVEAAKLF YIGGFHLTVS PDAIVKLGQH 

       190        200        210        220        230        240 
AKENSKPFVL NFSAPFIPHV FKDALARVLP YATVIIANES EAEAFCDAFQ LDCANTDLEA 

       250        260        270        280        290        300 
IAQRIVKDSP VEKTVIFTHG VEPTVVVSSK GTSTYPVKPL DSSKIVDTNG AGDAFAGGFM 

       310        320        330        340 
AGLTKGEDLE TSIDMGQWLA ALSIQEVGPS YPSEKISYSK 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 96604 / S288c / FY1679.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Role of adenosine kinase in Saccharomyces cerevisiae: identification of the ADO1 gene and study of the mutant phenotypes."
Lecoq K., Belloc I., Desgranges C., Daignan-Fornier B.
Yeast 18:335-342(2001) [PubMed: 11223943] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"Expression in Escherichia coli of a recombinant adenosine kinase from Saccharomyces cerevisiae: purification, kinetics and substrate analyses."
Barrado P., Rodriguez M.J., Jimenez A., Lobato M.F.
Yeast 20:1145-1150(2003) [PubMed: 14558146] [Abstract]
Cited for: CHARACTERIZATION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49605 Genomic DNA. Translation: CAA89635.1.
AY558082 Genomic DNA. Translation: AAS56408.1.
BK006943 Genomic DNA. Translation: DAA08890.1.
PIRS57126.
RefSeqNP_012639.1. NM_001181763.1.

3D structure databases

ProteinModelPortalP47143.
SMRP47143. Positions 1-335.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-4681N.
IntActP47143. 6 interactions.
MINTMINT-542654.
STRINGP47143.

Proteomic databases

PeptideAtlasP47143.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR105W; YJR105W; YJR105W.
GeneID853569.
KEGGsce:YJR105W.
NMPDRfig|4932.3.peg.3613.

Organism-specific databases

CYGDYJR105w.
SGDS000003866. ADO1.

Phylogenomic databases

eggNOGfuNOG07084.
GeneTreeEFGT00050000002260.
HOGENOMHBG314716.
OMAAQWMIQQ.
OrthoDBEOG4004T0.

Gene expression databases

ArrayExpressP47143.
GenevestigatorP47143.
GermOnlineYJR105W. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR001805. Adenokinase.
IPR011611. Carb/pur_kinase.
IPR002173. Carboh/pur_kinase_PfkB_CS.
[Graphical view]
KOK00856.
PANTHERPTHR10584:SF24. Adenokinase. 1 hit.
PfamPF00294. PfkB. 1 hit.
[Graphical view]
PRINTSPR00989. ADENOKINASE.
PROSITEPS00583. PFKB_KINASES_1. False negative.
PS00584. PFKB_KINASES_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974337.

Entry information

Entry nameADK_YEAST
AccessionPrimary (citable) accession number: P47143
Secondary accession number(s): D6VWS4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: December 14, 2011
This is version 106 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families