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Protein

Vacuolar protein-sorting-associated protein 25

Gene

VPS25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: GO_Central
  • structural molecule activity Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
  • protein targeting to vacuole Source: SGD
  • protein targeting to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31730-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 25
Alternative name(s):
ESCRT-II complex subunit VPS25
Gene namesi
Name:VPS25
Synonyms:VPT25
Ordered Locus Names:YJR102C
ORF Names:J1957
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR102C.
SGDiS000003863. VPS25.

Subcellular locationi

GO - Cellular componenti

  • ESCRT II complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152211 – 202Vacuolar protein-sorting-associated protein 25Add BLAST202

Proteomic databases

PRIDEiP47142.

Interactioni

Subunit structurei

Homodimer. Component of the endosomal sorting complex required for transport II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex.4 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
SNF8Q124834EBI-25595,EBI-30277
VPS20Q042723EBI-25595,EBI-28157
VPS36Q066963EBI-25595,EBI-36540

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33858. 66 interactors.
DIPiDIP-4446N.
IntActiP47142. 8 interactors.
MINTiMINT-486583.

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi11 – 13Combined sources3
Helixi20 – 40Combined sources21
Beta strandi45 – 47Combined sources3
Beta strandi50 – 52Combined sources3
Turni79 – 82Combined sources4
Helixi87 – 99Combined sources13
Beta strandi102 – 111Combined sources10
Beta strandi120 – 123Combined sources4
Helixi128 – 140Combined sources13
Helixi151 – 154Combined sources4
Turni164 – 167Combined sources4
Helixi170 – 181Combined sources12
Helixi182 – 184Combined sources3
Beta strandi187 – 190Combined sources4
Beta strandi196 – 200Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60C/D1-202[»]
1W7PX-ray3.60B/C1-202[»]
1XB4X-ray3.10A/B/C/D1-202[»]
ProteinModelPortaliP47142.
SMRiP47142.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47142.

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS25 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014892.
HOGENOMiHOG000191978.
InParanoidiP47142.
KOiK12189.
OMAiKYLRHTN.
OrthoDBiEOG092C58X3.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13149. PTHR13149. 2 hits.
PfamiPF05871. ESCRT-II. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.

Sequencei

Sequence statusi: Complete.

P47142-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALPPVYSF PPLYTRQPNS LTRRQQISTW IDIISQYCKT KKIWYMSVDG
60 70 80 90 100
TVINDNELDS GSTDNDDSKK ISKNLFNNED IQRSVSQVFI DEIWSQMTKE
110 120 130 140 150
GKCLPIDQSG RRSSNTTTTR YFILWKSLDS WASLILQWFE DSGKLNQVIT
160 170 180 190 200
LYELSEGDET VNWEFHRMPE SLLYYCLKPL CDRNRATMLK DENDKVIAIK

VV
Length:202
Mass (Da):23,558
Last modified:February 1, 1996 - v1
Checksum:i6A4545A8BC772CA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49602 Genomic DNA. Translation: CAA89632.1.
BK006943 Genomic DNA. Translation: DAA08887.1.
PIRiS57123.
RefSeqiNP_012636.1. NM_001181760.1.

Genome annotation databases

EnsemblFungiiYJR102C; YJR102C; YJR102C.
GeneIDi853566.
KEGGisce:YJR102C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49602 Genomic DNA. Translation: CAA89632.1.
BK006943 Genomic DNA. Translation: DAA08887.1.
PIRiS57123.
RefSeqiNP_012636.1. NM_001181760.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60C/D1-202[»]
1W7PX-ray3.60B/C1-202[»]
1XB4X-ray3.10A/B/C/D1-202[»]
ProteinModelPortaliP47142.
SMRiP47142.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33858. 66 interactors.
DIPiDIP-4446N.
IntActiP47142. 8 interactors.
MINTiMINT-486583.

Proteomic databases

PRIDEiP47142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR102C; YJR102C; YJR102C.
GeneIDi853566.
KEGGisce:YJR102C.

Organism-specific databases

EuPathDBiFungiDB:YJR102C.
SGDiS000003863. VPS25.

Phylogenomic databases

GeneTreeiENSGT00390000014892.
HOGENOMiHOG000191978.
InParanoidiP47142.
KOiK12189.
OMAiKYLRHTN.
OrthoDBiEOG092C58X3.

Enzyme and pathway databases

BioCyciYEAST:G3O-31730-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP47142.
PROiP47142.

Family and domain databases

Gene3Di1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERiPTHR13149. PTHR13149. 2 hits.
PfamiPF05871. ESCRT-II. 1 hit.
[Graphical view]
SUPFAMiSSF46785. SSF46785. 2 hits.
ProtoNetiSearch...

Entry informationi

Entry nameiVPS25_YEAST
AccessioniPrimary (citable) accession number: P47142
Secondary accession number(s): D6VWS1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.