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P47142

- VPS25_YEAST

UniProt

P47142 - VPS25_YEAST

Protein

Vacuolar protein-sorting-associated protein 25

Gene

VPS25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 109 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex.1 Publication

    GO - Molecular functioni

    1. protein binding Source: IntAct
    2. structural molecule activity Source: SGD

    GO - Biological processi

    1. negative regulation of transcription from RNA polymerase II promoter by glucose Source: SGD
    2. protein targeting to vacuole Source: SGD
    3. ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

    Keywords - Biological processi

    Protein transport, Transport

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31730-MONOMER.
    ReactomeiREACT_189189. Endosomal Sorting Complex Required For Transport (ESCRT).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Vacuolar protein-sorting-associated protein 25
    Alternative name(s):
    ESCRT-II complex subunit VPS25
    Gene namesi
    Name:VPS25
    Synonyms:VPT25
    Ordered Locus Names:YJR102C
    ORF Names:J1957
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR102c.
    SGDiS000003863. VPS25.

    Subcellular locationi

    Cytoplasm 1 Publication. Endosome membrane 1 Publication; Peripheral membrane protein 1 Publication

    GO - Cellular componenti

    1. ESCRT II complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Endosome, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 202202Vacuolar protein-sorting-associated protein 25PRO_0000215221Add
    BLAST

    Proteomic databases

    PaxDbiP47142.

    Expressioni

    Gene expression databases

    GenevestigatoriP47142.

    Interactioni

    Subunit structurei

    Homodimer. Component of the endosomal sorting complex required for transport II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex.4 Publications

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    SNF8Q124834EBI-25595,EBI-30277
    VPS20Q042723EBI-25595,EBI-28157
    VPS36Q066963EBI-25595,EBI-36540

    Protein-protein interaction databases

    BioGridi33858. 58 interactions.
    DIPiDIP-4446N.
    IntActiP47142. 8 interactions.
    MINTiMINT-486583.
    STRINGi4932.YJR102C.

    Structurei

    Secondary structure

    1
    202
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi7 – 93
    Helixi11 – 133
    Helixi20 – 4021
    Beta strandi45 – 473
    Beta strandi50 – 523
    Turni79 – 824
    Helixi87 – 9913
    Beta strandi102 – 11110
    Beta strandi120 – 1234
    Helixi128 – 14013
    Helixi151 – 1544
    Turni164 – 1674
    Helixi170 – 18112
    Helixi182 – 1843
    Beta strandi187 – 1904
    Beta strandi196 – 2005

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1U5TX-ray3.60C/D1-202[»]
    1W7PX-ray3.60B/C1-202[»]
    1XB4X-ray3.10A/B/C/D1-202[»]
    ProteinModelPortaliP47142.
    SMRiP47142. Positions 1-199.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP47142.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the VPS25 family.Curated

    Phylogenomic databases

    eggNOGiNOG270942.
    GeneTreeiENSGT00390000014892.
    HOGENOMiHOG000191978.
    KOiK12189.
    OMAiPPFFTIQ.
    OrthoDBiEOG7QRR76.

    Family and domain databases

    Gene3Di1.10.10.10. 1 hit.
    1.10.10.570. 1 hit.
    InterProiIPR008570. ESCRT-II_cplx_vps25-sub.
    IPR014041. ESCRT-II_cplx_Vps25-sub_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view]
    PANTHERiPTHR13149. PTHR13149. 1 hit.
    PfamiPF05871. ESCRT-II. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47142-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSALPPVYSF PPLYTRQPNS LTRRQQISTW IDIISQYCKT KKIWYMSVDG    50
    TVINDNELDS GSTDNDDSKK ISKNLFNNED IQRSVSQVFI DEIWSQMTKE 100
    GKCLPIDQSG RRSSNTTTTR YFILWKSLDS WASLILQWFE DSGKLNQVIT 150
    LYELSEGDET VNWEFHRMPE SLLYYCLKPL CDRNRATMLK DENDKVIAIK 200
    VV 202
    Length:202
    Mass (Da):23,558
    Last modified:February 1, 1996 - v1
    Checksum:i6A4545A8BC772CA2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49602 Genomic DNA. Translation: CAA89632.1.
    BK006943 Genomic DNA. Translation: DAA08887.1.
    PIRiS57123.
    RefSeqiNP_012636.1. NM_001181760.1.

    Genome annotation databases

    EnsemblFungiiYJR102C; YJR102C; YJR102C.
    GeneIDi853566.
    KEGGisce:YJR102C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49602 Genomic DNA. Translation: CAA89632.1 .
    BK006943 Genomic DNA. Translation: DAA08887.1 .
    PIRi S57123.
    RefSeqi NP_012636.1. NM_001181760.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1U5T X-ray 3.60 C/D 1-202 [» ]
    1W7P X-ray 3.60 B/C 1-202 [» ]
    1XB4 X-ray 3.10 A/B/C/D 1-202 [» ]
    ProteinModelPortali P47142.
    SMRi P47142. Positions 1-199.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33858. 58 interactions.
    DIPi DIP-4446N.
    IntActi P47142. 8 interactions.
    MINTi MINT-486583.
    STRINGi 4932.YJR102C.

    Proteomic databases

    PaxDbi P47142.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR102C ; YJR102C ; YJR102C .
    GeneIDi 853566.
    KEGGi sce:YJR102C.

    Organism-specific databases

    CYGDi YJR102c.
    SGDi S000003863. VPS25.

    Phylogenomic databases

    eggNOGi NOG270942.
    GeneTreei ENSGT00390000014892.
    HOGENOMi HOG000191978.
    KOi K12189.
    OMAi PPFFTIQ.
    OrthoDBi EOG7QRR76.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31730-MONOMER.
    Reactomei REACT_189189. Endosomal Sorting Complex Required For Transport (ESCRT).

    Miscellaneous databases

    EvolutionaryTracei P47142.
    NextBioi 974328.
    PROi P47142.

    Gene expression databases

    Genevestigatori P47142.

    Family and domain databases

    Gene3Di 1.10.10.10. 1 hit.
    1.10.10.570. 1 hit.
    InterProi IPR008570. ESCRT-II_cplx_vps25-sub.
    IPR014041. ESCRT-II_cplx_Vps25-sub_N.
    IPR011991. WHTH_DNA-bd_dom.
    [Graphical view ]
    PANTHERi PTHR13149. PTHR13149. 1 hit.
    Pfami PF05871. ESCRT-II. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    3. "Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body."
      Babst M., Katzmann D.J., Snyder W.B., Wendland B., Emr S.D.
      Dev. Cell 3:283-289(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
    4. "Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II."
      Wernimont A.K., Weissenhorn W.
      BMC Struct. Biol. 4:10-10(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS), SUBUNIT.
    5. "ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes."
      Teo H., Perisic O., Gonzalez B., Williams R.L.
      Dev. Cell 7:559-569(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH SNF8 AND VPS36.
    6. "Structure of the ESCRT-II endosomal trafficking complex."
      Hierro A., Sun J., Rusnak A.S., Kim J., Prag G., Emr S.D., Hurley J.H.
      Nature 431:221-225(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH SNF8 AND VPS36.

    Entry informationi

    Entry nameiVPS25_YEAST
    AccessioniPrimary (citable) accession number: P47142
    Secondary accession number(s): D6VWS1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 109 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families
    3. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    4. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3