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Protein

Vacuolar protein-sorting-associated protein 25

Gene

VPS25

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex.1 Publication

GO - Molecular functioni

  • protein homodimerization activity Source: GO_Central
  • structural molecule activity Source: SGD

GO - Biological processi

  • ATP export Source: SGD
  • carbon catabolite repression of transcription from RNA polymerase II promoter by glucose Source: SGD
  • protein targeting to vacuole Source: SGD
  • protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: GO_Central
  • ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway Source: SGD

Keywordsi

Biological processProtein transport, Transport

Enzyme and pathway databases

BioCyciYEAST:G3O-31730-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Vacuolar protein-sorting-associated protein 25
Alternative name(s):
ESCRT-II complex subunit VPS25
Gene namesi
Name:VPS25
Synonyms:VPT25
Ordered Locus Names:YJR102C
ORF Names:J1957
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR102C
SGDiS000003863 VPS25

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002152211 – 202Vacuolar protein-sorting-associated protein 25Add BLAST202

Proteomic databases

PaxDbiP47142
PRIDEiP47142

Interactioni

Subunit structurei

Homodimer. Component of the endosomal sorting complex required for transport II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex.4 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi33858, 108 interactors
DIPiDIP-4446N
IntActiP47142, 8 interactors
MINTiP47142
STRINGi4932.YJR102C

Structurei

Secondary structure

1202
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 9Combined sources3
Helixi11 – 13Combined sources3
Helixi20 – 40Combined sources21
Beta strandi45 – 47Combined sources3
Beta strandi50 – 52Combined sources3
Turni79 – 82Combined sources4
Helixi87 – 99Combined sources13
Beta strandi102 – 111Combined sources10
Beta strandi120 – 123Combined sources4
Helixi128 – 140Combined sources13
Helixi151 – 154Combined sources4
Turni164 – 167Combined sources4
Helixi170 – 181Combined sources12
Helixi182 – 184Combined sources3
Beta strandi187 – 190Combined sources4
Beta strandi196 – 200Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60C/D1-202[»]
1W7PX-ray3.60B/C1-202[»]
1XB4X-ray3.10A/B/C/D1-202[»]
ProteinModelPortaliP47142
SMRiP47142
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP47142

Family & Domainsi

Sequence similaritiesi

Belongs to the VPS25 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000014892
HOGENOMiHOG000191978
InParanoidiP47142
KOiK12189
OMAiPPFFTIQ
OrthoDBiEOG092C58X3

Family and domain databases

Gene3Di1.10.10.10, 1 hit
1.10.10.570, 1 hit
InterProiView protein in InterPro
IPR008570 ESCRT-II_cplx_Vps25-sub
IPR014041 ESCRT-II_cplx_Vps25-sub_N
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13149 PTHR13149, 1 hit
PfamiView protein in Pfam
PF05871 ESCRT-II, 1 hit
SUPFAMiSSF46785 SSF46785, 2 hits

Sequencei

Sequence statusi: Complete.

P47142-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSALPPVYSF PPLYTRQPNS LTRRQQISTW IDIISQYCKT KKIWYMSVDG
60 70 80 90 100
TVINDNELDS GSTDNDDSKK ISKNLFNNED IQRSVSQVFI DEIWSQMTKE
110 120 130 140 150
GKCLPIDQSG RRSSNTTTTR YFILWKSLDS WASLILQWFE DSGKLNQVIT
160 170 180 190 200
LYELSEGDET VNWEFHRMPE SLLYYCLKPL CDRNRATMLK DENDKVIAIK

VV
Length:202
Mass (Da):23,558
Last modified:February 1, 1996 - v1
Checksum:i6A4545A8BC772CA2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49602 Genomic DNA Translation: CAA89632.1
BK006943 Genomic DNA Translation: DAA08887.1
PIRiS57123
RefSeqiNP_012636.1, NM_001181760.1

Genome annotation databases

EnsemblFungiiYJR102C; YJR102C; YJR102C
GeneIDi853566
KEGGisce:YJR102C

Similar proteinsi

Entry informationi

Entry nameiVPS25_YEAST
AccessioniPrimary (citable) accession number: P47142
Secondary accession number(s): D6VWS1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

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