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P47142 (VPS25_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Vacuolar protein-sorting-associated protein 25
Alternative name(s):
ESCRT-II complex subunit VPS25
Gene names
Name:VPS25
Synonyms:VPT25
Ordered Locus Names:YJR102C
ORF Names:J1957
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length202 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the ESCRT-II complex (endosomal sorting complex required for transport II), which is required for multivesicular body (MVB) formation and sorting of endosomal cargo proteins into MVBs. The MVB pathway mediates delivery of transmembrane proteins into the lumen of the lysosome for degradation. The ESCRT-II complex is probably involved in the recruitment of the ESCRT-III complex. Ref.3

Subunit structure

Homodimer. Component of the endosomal sorting complex required for transport II (ESCRT-II), which consists of 2 copies of VPS25, 1 copy of SNF8, and 1 copy of VPS36. The ESCRT-II complex interacts directly with the VPS20 subunit of the ESCRT-III complex. Ref.3 Ref.4

Subcellular location

Cytoplasm. Endosome membrane; Peripheral membrane protein Ref.3.

Sequence similarities

Belongs to the VPS25 family.

Binary interactions

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 202202Vacuolar protein-sorting-associated protein 25
PRO_0000215221

Secondary structure

................................ 202
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P47142 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 6A4545A8BC772CA2

FASTA20223,558
        10         20         30         40         50         60 
MSALPPVYSF PPLYTRQPNS LTRRQQISTW IDIISQYCKT KKIWYMSVDG TVINDNELDS 

        70         80         90        100        110        120 
GSTDNDDSKK ISKNLFNNED IQRSVSQVFI DEIWSQMTKE GKCLPIDQSG RRSSNTTTTR 

       130        140        150        160        170        180 
YFILWKSLDS WASLILQWFE DSGKLNQVIT LYELSEGDET VNWEFHRMPE SLLYYCLKPL 

       190        200 
CDRNRATMLK DENDKVIAIK VV 

« Hide

References

« Hide 'large scale' references
[1]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[3]"Endosome-associated complex, ESCRT-II, recruits transport machinery for protein sorting at the multivesicular body."
Babst M., Katzmann D.J., Snyder W.B., Wendland B., Emr S.D.
Dev. Cell 3:283-289(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT, SUBCELLULAR LOCATION.
[4]"Crystal structure of subunit VPS25 of the endosomal trafficking complex ESCRT-II."
Wernimont A.K., Weissenhorn W.
BMC Struct. Biol. 4:10-10(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.1 ANGSTROMS), SUBUNIT.
[5]"ESCRT-II, an endosome-associated complex required for protein sorting: crystal structure and interactions with ESCRT-III and membranes."
Teo H., Perisic O., Gonzalez B., Williams R.L.
Dev. Cell 7:559-569(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH SNF8 AND VPS36.
[6]"Structure of the ESCRT-II endosomal trafficking complex."
Hierro A., Sun J., Rusnak A.S., Kim J., Prag G., Emr S.D., Hurley J.H.
Nature 431:221-225(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (3.6 ANGSTROMS) IN COMPLEX WITH SNF8 AND VPS36.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49602 Genomic DNA. Translation: CAA89632.1.
BK006943 Genomic DNA. Translation: DAA08887.1.
PIRS57123.
RefSeqNP_012636.1. NM_001181760.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1U5TX-ray3.60C/D1-202[»]
1W7PX-ray3.60B/C1-202[»]
1XB4X-ray3.10A/B/C/D1-202[»]
ProteinModelPortalP47142.
SMRP47142. Positions 1-199.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33858. 58 interactions.
DIPDIP-4446N.
IntActP47142. 8 interactions.
MINTMINT-486583.
STRING4932.YJR102C.

Proteomic databases

PaxDbP47142.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR102C; YJR102C; YJR102C.
GeneID853566.
KEGGsce:YJR102C.

Organism-specific databases

CYGDYJR102c.
SGDS000003863. VPS25.

Phylogenomic databases

eggNOGNOG270942.
GeneTreeENSGT00390000014892.
HOGENOMHOG000191978.
KOK12189.
OMAPPFFTIQ.
OrthoDBEOG7QRR76.

Enzyme and pathway databases

BioCycYEAST:G3O-31730-MONOMER.

Gene expression databases

GenevestigatorP47142.

Family and domain databases

Gene3D1.10.10.10. 1 hit.
1.10.10.570. 1 hit.
InterProIPR008570. ESCRT-II_cplx_vps25-sub.
IPR014041. ESCRT-II_cplx_Vps25-sub_N.
IPR011991. WHTH_DNA-bd_dom.
[Graphical view]
PANTHERPTHR13149. PTHR13149. 1 hit.
PfamPF05871. ESCRT-II. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP47142.
NextBio974328.
PROP47142.

Entry information

Entry nameVPS25_YEAST
AccessionPrimary (citable) accession number: P47142
Secondary accession number(s): D6VWS1
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references