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Protein

Diphthamide biosynthesis protein 4

Gene

JJJ3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.1 Publication

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri91 – 16474DPH-typePROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  • ferrous iron binding Source: SGD

GO - Biological processi

  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15581.
YEAST:G3O-31725-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthamide biosynthesis protein 4
Alternative name(s):
J protein type 3
Gene namesi
Name:JJJ3
Synonyms:DPH4
Ordered Locus Names:YJR097W
ORF Names:J1931
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR097W.
SGDiS000003858. JJJ3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedCombined sources
Chaini2 – 172171Diphthamide biosynthesis protein 4PRO_0000071154Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserineCombined sources

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP47138.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
DPH1P404871EBI-25576,EBI-25162
YNL092WP539341EBI-25576,EBI-28794

Protein-protein interaction databases

BioGridi33852. 48 interactions.
DIPiDIP-4356N.
IntActiP47138. 2 interactions.
MINTiMINT-472665.

Structurei

3D structure databases

ProteinModelPortaliP47138.
SMRiP47138. Positions 9-73.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 7772JPROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the DPH4 family.Curated
Contains 1 DPH-type zinc finger.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri91 – 16474DPH-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000189813.
InParanoidiP47138.
KOiK17867.
OMAiSCSLWIQ.
OrthoDBiEOG7HXD45.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR007872. Znf_DHP.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF05207. zf-CSL. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51074. ZF_DPH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47138-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVNSLTHY EILRIPSDAT QDEIKKAYRN RLLNTHPDKL SKSIHDTVSN
60 70 80 90 100
VTINKIQDAY KILSNIKTRR EYDRLILENY KRQGFHNCGD GLDEFSLDDF
110 120 130 140 150
SFDEDKLEFM MNCPRCQFVG GFHFSESLLD ECIDNVDAME RSHSGYQLLT
160 170
QCSACSLWLK VNFDIEEEQE GQ
Length:172
Mass (Da):20,027
Last modified:February 1, 1996 - v1
Checksum:i3BE6567ABC35EFF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49597 Genomic DNA. Translation: CAA89627.1.
AY558542 Genomic DNA. Translation: AAS56868.1.
BK006943 Genomic DNA. Translation: DAA08882.1.
PIRiS57118.
RefSeqiNP_012631.3. NM_001181755.3.

Genome annotation databases

EnsemblFungiiYJR097W; YJR097W; YJR097W.
GeneIDi853560.
KEGGisce:YJR097W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49597 Genomic DNA. Translation: CAA89627.1.
AY558542 Genomic DNA. Translation: AAS56868.1.
BK006943 Genomic DNA. Translation: DAA08882.1.
PIRiS57118.
RefSeqiNP_012631.3. NM_001181755.3.

3D structure databases

ProteinModelPortaliP47138.
SMRiP47138. Positions 9-73.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33852. 48 interactions.
DIPiDIP-4356N.
IntActiP47138. 2 interactions.
MINTiMINT-472665.

Proteomic databases

MaxQBiP47138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR097W; YJR097W; YJR097W.
GeneIDi853560.
KEGGisce:YJR097W.

Organism-specific databases

EuPathDBiFungiDB:YJR097W.
SGDiS000003858. JJJ3.

Phylogenomic databases

HOGENOMiHOG000189813.
InParanoidiP47138.
KOiK17867.
OMAiSCSLWIQ.
OrthoDBiEOG7HXD45.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-15581.
YEAST:G3O-31725-MONOMER.

Miscellaneous databases

NextBioi974311.
PROiP47138.

Family and domain databases

Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR007872. Znf_DHP.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF05207. zf-CSL. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51074. ZF_DPH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. "The J-protein family: modulating protein assembly, disassembly and translocation."
    Walsh P., Bursac D., Law Y.C., Cyr D., Lithgow T.
    EMBO Rep. 5:567-571(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE NAME.
  5. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Identification of the proteins required for biosynthesis of diphthamide, the target of bacterial ADP-ribosylating toxins on translation elongation factor 2."
    Liu S., Milne G.T., Kuremsky J.G., Fink G.R., Leppla S.H.
    Mol. Cell. Biol. 24:9487-9497(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS], IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiDPH4_YEAST
AccessioniPrimary (citable) accession number: P47138
Secondary accession number(s): D6VWR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.