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Protein

Diphthamide biosynthesis protein 4

Gene

JJJ3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the first step of diphthamide biosynthesis, the transfer of 3-amino-3-carboxypropyl from S-adenosyl-L-methionine to a histidine residue. Diphthamide is a post-translational modification of histidine which occurs in elongation factor 2.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri91 – 164DPH-typePROSITE-ProRule annotationAdd BLAST74

GO - Molecular functioni

  • ferrous iron binding Source: SGD

GO - Biological processi

  • peptidyl-diphthamide biosynthetic process from peptidyl-histidine Source: SGD
Complete GO annotation...

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31725-MONOMER.
YEAST:G3O-31725-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Diphthamide biosynthesis protein 4
Alternative name(s):
J protein type 3
Gene namesi
Name:JJJ3
Synonyms:DPH4
Ordered Locus Names:YJR097W
ORF Names:J1931
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR097W.
SGDiS000003858. JJJ3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000711542 – 172Diphthamide biosynthesis protein 4Add BLAST171

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineCombined sources1

Keywords - PTMi

Acetylation

Proteomic databases

MaxQBiP47138.
PRIDEiP47138.

Interactioni

Protein-protein interaction databases

BioGridi33852. 49 interactors.
DIPiDIP-4356N.
IntActiP47138. 2 interactors.
MINTiMINT-472665.

Structurei

3D structure databases

ProteinModelPortaliP47138.
SMRiP47138.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 77JPROSITE-ProRule annotationAdd BLAST72

Sequence similaritiesi

Belongs to the DPH4 family.Curated
Contains 1 DPH-type zinc finger.PROSITE-ProRule annotation
Contains 1 J domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri91 – 164DPH-typePROSITE-ProRule annotationAdd BLAST74

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOGENOMiHOG000189813.
InParanoidiP47138.
KOiK17867.
OMAiCNSCSLW.
OrthoDBiEOG092C4Z2R.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR007872. Znf_DHP.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF05207. zf-CSL. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51074. ZF_DPH. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47138-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLVNSLTHY EILRIPSDAT QDEIKKAYRN RLLNTHPDKL SKSIHDTVSN
60 70 80 90 100
VTINKIQDAY KILSNIKTRR EYDRLILENY KRQGFHNCGD GLDEFSLDDF
110 120 130 140 150
SFDEDKLEFM MNCPRCQFVG GFHFSESLLD ECIDNVDAME RSHSGYQLLT
160 170
QCSACSLWLK VNFDIEEEQE GQ
Length:172
Mass (Da):20,027
Last modified:February 1, 1996 - v1
Checksum:i3BE6567ABC35EFF3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49597 Genomic DNA. Translation: CAA89627.1.
AY558542 Genomic DNA. Translation: AAS56868.1.
BK006943 Genomic DNA. Translation: DAA08882.1.
PIRiS57118.
RefSeqiNP_012631.3. NM_001181755.3.

Genome annotation databases

EnsemblFungiiYJR097W; YJR097W; YJR097W.
GeneIDi853560.
KEGGisce:YJR097W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49597 Genomic DNA. Translation: CAA89627.1.
AY558542 Genomic DNA. Translation: AAS56868.1.
BK006943 Genomic DNA. Translation: DAA08882.1.
PIRiS57118.
RefSeqiNP_012631.3. NM_001181755.3.

3D structure databases

ProteinModelPortaliP47138.
SMRiP47138.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33852. 49 interactors.
DIPiDIP-4356N.
IntActiP47138. 2 interactors.
MINTiMINT-472665.

Proteomic databases

MaxQBiP47138.
PRIDEiP47138.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR097W; YJR097W; YJR097W.
GeneIDi853560.
KEGGisce:YJR097W.

Organism-specific databases

EuPathDBiFungiDB:YJR097W.
SGDiS000003858. JJJ3.

Phylogenomic databases

HOGENOMiHOG000189813.
InParanoidiP47138.
KOiK17867.
OMAiCNSCSLW.
OrthoDBiEOG092C4Z2R.

Enzyme and pathway databases

BioCyciMetaCyc:G3O-31725-MONOMER.
YEAST:G3O-31725-MONOMER.

Miscellaneous databases

PROiP47138.

Family and domain databases

CDDicd06257. DnaJ. 1 hit.
Gene3Di1.10.287.110. 1 hit.
InterProiIPR001623. DnaJ_domain.
IPR007872. Znf_DHP.
[Graphical view]
PfamiPF00226. DnaJ. 1 hit.
PF05207. zf-CSL. 1 hit.
[Graphical view]
PRINTSiPR00625. JDOMAIN.
SMARTiSM00271. DnaJ. 1 hit.
[Graphical view]
SUPFAMiSSF46565. SSF46565. 1 hit.
PROSITEiPS50076. DNAJ_2. 1 hit.
PS51074. ZF_DPH. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDPH4_YEAST
AccessioniPrimary (citable) accession number: P47138
Secondary accession number(s): D6VWR6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2010 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.