Protein JSN1 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47135 (JSN1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 116. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Protein JSN1

Alternative name(s):
Pumilio homology domain family member 1

Gene names

Name:	JSN1
Synonyms:	PUF1
Ordered Locus Names:	YJR091C
ORF Names:	J1890

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces ›

Protein attributes

Sequence length	1091 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Miscellaneous

Present with 784 molecules/cell in log phase SD medium.

Sequence similarities

Contains 1 PUM-HD domain.

Contains 5 pumilio repeats.

Contains 1 RRM (RNA recognition motif) domain.

Ontologies

Keywords
Domain	Repeat
Ligand	RNA-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	cellular protein complex localization Inferred from mutant phenotype PubMed 16107558. Source: SGD establishment of mitochondrion localization Inferred from mutant phenotype PubMed 16107558. Source: SGD nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay Inferred from genetic interaction PubMed 11101532. Source: SGD
Cellular_component	external side of mitochondrial outer membrane Inferred from direct assay PubMed 16107558. Source: SGD
Molecular_function	mRNA binding Inferred from direct assay PubMed 15024427. Source: SGD nucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1091

1091

Protein JSN1

PRO_0000075922

Regions

Domain

340 – 426

RRM

Domain

557 – 913

357

PUM-HD

Repeat

617 – 652

Pumilio 1

Repeat

653 – 689

Pumilio 2

Repeat

690 – 724

Pumilio 3

Repeat

725 – 760

Pumilio 4

Repeat

801 – 837

Pumilio 5

Compositional bias

507 – 510

Poly-Asn

Compositional bias

515 – 518

Poly-Asn

Compositional bias

923 – 927

Poly-Gln

Compositional bias

967 – 970

Poly-Thr

Compositional bias

1065 – 1076

Poly-Asn

Amino acid modifications

Modified residue

129

Phosphoserine Ref.7

Modified residue

131

Phosphothreonine Ref.7

Modified residue

157

Phosphoserine Ref.6 Ref.7

Modified residue

158

Phosphoserine Ref.7

Modified residue

160

Phosphoserine Ref.7

Modified residue

273

Phosphoserine Ref.7

Modified residue

913

Phosphoserine Ref.6 Ref.7

Modified residue

916

Phosphoserine Ref.7

Modified residue

951

Phosphoserine Ref.7

Modified residue

954

Phosphoserine Ref.7

Sequences

Sequence

Length

Mass (Da)

Tools

P47135 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: A168489189C67B22
Show »

FASTA

1,091

120,374

        10         20         30         40         50         60 
MDKSKQMNIN NLSNIPEVID PGITIPIYEE EYENNGESNS QLQQQPQKLG SYRSRAGKFS 

        70         80         90        100        110        120 
NTLSNLLPSI SAKLHHSKKN SHGKNGAEFS SSNNSSQSTV ASKTPRASPS RSKMMESSID 

       130        140        150        160        170        180 
GVTMDRPGSL TPPQDMEKLV HFPDSSNNFL IPAPRGSSDS FNLPHQISRT RNNTMSSQIT 

       190        200        210        220        230        240 
SISSIAPKPR TSSGIWSSNA SANDPMQQHL LQQLQPTTSN NTTNSNTLND YSTKTAYFDN 

       250        260        270        280        290        300 
MVSTSGSQMA DNKMNTNNLA IPNSVWSNTR QRSQSNASSI YTDAPLYEQP ARASISSHYT 

       310        320        330        340        350        360 
IPTQESPLIA DEIDPQSINW VTMDPTVPSI NQISNLLPTN TISISNVFPL QHQQPQLNNA 

       370        380        390        400        410        420 
INLTSTSLAT LCSKYGEVIS ARTLRNLNMA LVEFSSVESA VKALDSLQGK EVSMIGAPSK 

       430        440        450        460        470        480 
ISFAKILPMH QQPPQFLLNS QGLPLGLENN NLQPQPLLQE QLFNGAVTFQ QQGNVSIPVF 

       490        500        510        520        530        540 
NQQSQQSQHQ NHSSGSAGFS NVLHGYNNNN SMHGNNNNSA NEKEQCPFPL PPPNVNEKED 

       550        560        570        580        590        600 
LLREIIELFE ANSDEYQINS LIKKSLNHKG TSDTQNFGPL PEPLSGREFD PPKLRELRKS 

       610        620        630        640        650        660 
IDSNAFSDLE IEQLAIAMLD ELPELSSDYL GNTIVQKLFE HSSDIIKDIM LRKTSKYLTS 

       670        680        690        700        710        720 
MGVHKNGTWA CQKMITMAHT PRQIMQVTQG VKDYCTPLIN DQFGNYVIQC VLKFGFPWNQ 

       730        740        750        760        770        780 
FIFESIIANF WVIVQNRYGA RAVRACLEAH DIVTPEQSIV LSAMIVTYAE YLSTNSNGAL 

       790        800        810        820        830        840 
LVTWFLDTSV LPNRHSILAP RLTKRIVELC GHRLASLTIL KVLNYRGDDN ARKIILDSLF 

       850        860        870        880        890        900 
GNVNAHDSSP PKELTKLLCE TNYGPTFVHK VLAMPLLEDD LRAHIIKQVR KVLTDSTQIQ 

       910        920        930        940        950        960 
PSRRLLEEVG LASPSSTHNK TKQQQQQHHN SSISHMFATP DTSGQHMRGL SVSSVKSGGS 

       970        980        990       1000       1010       1020 
KHTTMNTTTT NGSSASTLSP GQPLNANSNS SMGYFSYPGV FPVSGFSGNA SNGYAMNNDD 

      1030       1040       1050       1060       1070       1080 
LSSQFDMLNF NNGTRLSLPQ LSLTNHNNTT MELVNNVGSS QPHTNNNNNN NNTNYNDDNT 

      1090 
VFETLTLHSA N

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant." Machin N., Lee J.S., Barnes G. Mol. Biol. Cell 6:1241-1259(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 204-1091. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-157 AND SER-913, MASS SPECTROMETRY.
[7]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-129; THR-131; SER-157; SER-158; SER-160; SER-273; SER-913; SER-916; SER-951 AND SER-954, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L43493 Genomic DNA. Translation: AAC41666.1. Z49591 Genomic DNA. Translation: CAA89618.1. L47993 Genomic DNA. Translation: AAB39314.1. BK006943 Genomic DNA. Translation: DAA08875.1.
PIR	S57112.
RefSeq	NP_012624.1. NM_001181748.1.
3D structure databases
ProteinModelPortal	P47135.
SMR	P47135. Positions 620-850.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-1281N.
IntAct	P47135. 37 interactions.
MINT	MINT-405099.
STRING	4932.YJR091C.
Proteomic databases
PaxDb	P47135.
PeptideAtlas	P47135.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR091C; YJR091C; YJR091C.
GeneID	853553.
KEGG	sce:YJR091C.
Organism-specific databases
CYGD	YJR091c.
SGD	S000003851. JSN1.
Phylogenomic databases
eggNOG	COG5099.
GeneTree	ENSGT00690000103065.
HOGENOM	HOG000113136.
OMA	CLEAHDI.
OrthoDB	EOG4MWCFG.
Gene expression databases
Genevestigator	P47135.
GermOnline	YJR091C. Saccharomyces cerevisiae.
Family and domain databases
Gene3D	1.25.10.10. 1 hit. 3.30.70.330. 1 hit.
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR012677. Nucleotide-bd_a/b_plait. IPR001313. Pumilio_RNA-bd_rpt. IPR000504. RRM_dom. [Graphical view]
Pfam	PF00806. PUF. 3 hits. PF00076. RRM_1. 1 hit. [Graphical view]
SMART	SM00025. Pumilio. 6 hits. SM00360. RRM. 1 hit. [Graphical view]
SUPFAM	SSF48371. ARM-type_fold. 1 hit.
PROSITE	PS50302. PUM. 5 hits. PS50303. PUM_HD. 1 hit. PS50102. RRM. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974290.

Entry information

Entry name

JSN1_YEAST

Accession

Primary (citable) accession number: P47135
Secondary accession number(s): D6VWQ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 1, 2013
This is version 116 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents