P47131 (YJ55_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UPF0136 membrane protein YJR085C

Gene names

Ordered Locus Names:	YJR085C
ORF Names:	J1863

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	105 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Subcellular location	Mitochondrion. Membrane; Multi-pass membrane protein Potential Ref.6 Ref.7.
Induction	By the DNA-damaging agent methyl methanesulphonate (MMS) (at protein level). Ref.8
Miscellaneous	Present with 2100 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the UPF0136 (TMEM14) family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD mitochondrion Inferred from direct assay Ref.6Ref.7. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
TSA2	Q04120	1	EBI-25560,EBI-19631

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 105	105	UPF0136 membrane protein YJR085C		PRO_0000203105
Regions
	Transmembrane	26 – 46	21	Helical; Potential
	Transmembrane	53 – 73	21	Helical; Potential
	Transmembrane	77 – 97	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P47131 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: C35A1E8AB98CFFA0	FASTA	105	11,250
	10 20 30 40 50 60 MEHPAYTLSL LTTAGGLMGY YRKGSIPSLV SGLVFGSVYG IAGYLLHMNR DGGLEMALGA 70 80 90 100 STLLLGAGVI RGMPSRFTKP VPVVLTALGG LGSYYYYNKY KEFYP

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
[7]	"Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics." Reinders J., Zahedi R.P., Pfanner N., Meisinger C., Sickmann A. J. Proteome Res. 5:1543-1554(2006) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Global protein expression profiling of budding yeast in response to DNA damage." Lee M.-W., Kim B.-J., Choi H.-K., Ryu M.-J., Kim S.-B., Kang K.-M., Cho E.-J., Youn H.-D., Huh W.-K., Kim S.-T. Yeast 24:145-154(2007) [PubMed] [Europe PMC] [Abstract] Cited for: INDUCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49585 Genomic DNA. Translation: CAA89612.1. L47993 Genomic DNA. Translation: AAB39308.1. AY557887 Genomic DNA. Translation: AAS56213.1. BK006943 Genomic DNA. Translation: DAA08870.1.
PIR	S57104.
RefSeq	NP_012618.3. NM_001181742.3.
3D structure databases
ProteinModelPortal	P47131.
ModBase	Search...
Protein-protein interaction databases
IntAct	P47131. 2 interactions.
MINT	MINT-4491887.
STRING	4932.YJR085C.
Proteomic databases
PaxDb	P47131.
PeptideAtlas	P47131.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR085C; YJR085C; YJR085C.
GeneID	853547.
KEGG	sce:YJR085C.
Organism-specific databases
CYGD	YJR085c.
SGD	S000003845. YJR085C.
Phylogenomic databases
eggNOG	COG5548.
HOGENOM	HOG000211932.
OMA	KYKEFYP.
OrthoDB	EOG44QX9J.
Enzyme and pathway databases
BioCyc	YEAST:G3O-31713-MONOMER.
Gene expression databases
Genevestigator	P47131.
GermOnline	YJR085C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR005349. UPF0136_TM. [Graphical view]
PANTHER	PTHR12668. PTHR12668. 1 hit.
Pfam	PF03647. Tmemb_14. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974275.

Entry information

Entry name

YJ55_YEAST

Accession

Primary (citable) accession number: P47131
Secondary accession number(s): D6VWQ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	May 29, 2013
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program