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P47130 (CSN12_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 100. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cop9 signalosome complex subunit 12
Gene names
Name:CSN12
Ordered Locus Names:YJR084W
ORF Names:J1860
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length423 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Component of the COP9 signalosome (CSN) complex that acts as an regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunit of SCF-type E3 ubiquitin-protein ligase complexes. The CSN complex is involved in the regulation of the mating pheromone response. CSN12 forms a complex with THP3 that is recruited to transcribed genes and required for transcription elongation. Ref.4 Ref.6 Ref.8 Ref.9

Subunit structure

Component of a COP9 signalosome-like (CSN) complex, composed of RRI1/CSN5, CSN9, RRI2/CSN10, PCI8/CSN11, CSN12 and CSI1. In the complex, it probably interacts directly with RRI1/CSN5, CSN9, RRI2/CSN10 and CSI1. Interacts with SEM1 and THP3. Ref.4 Ref.5 Ref.6 Ref.8 Ref.9

Subcellular location

Cytoplasm. Nucleus Ref.7.

Miscellaneous

Present with 1510 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the CSN12 family.

Contains 1 PCI domain.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

RRI1Q124683EBI-763,EBI-37511
THP3Q120492EBI-763,EBI-34263

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 423423Cop9 signalosome complex subunit 12
PRO_0000121047

Regions

Domain298 – 415118PCI

Sequences

Sequence LengthMass (Da)Tools
P47130 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 750CDA631916A621

FASTA42349,482
        10         20         30         40         50         60 
MDVDIGCYFE EKRYDDKLLD FIRYDVKTPK KTKYILQRPT ATDEESVRLQ RFYQLGVDLK 

        70         80         90        100        110        120 
LKYSKRRSLK KQGRIKNATE ELLRLANEQL KLFNRIVERE TNWIIYPLWV MAKQLIRLAN 

       130        140        150        160        170        180 
ESSELNKDSI EECGRTIHRS FTICLNDRNP RLNENKKIGC YMFANLEFSI YHRLSNKDMI 

       190        200        210        220        230        240 
KNLVKVLESR VNARDIPPLN KSLAMEHKSQ VVLYNYYLGQ YYGCLENDHE RGFFHLNEAL 

       250        260        270        280        290        300 
LQCPMLYVES TGKFVLQGQM EKIMILLVPL ALLTKRLYPH WDHPVIAGVI TRSKRLSQVY 

       310        320        330        340        350        360 
PTLVRSVISG NLSLYEATAA SHERFFLSQG LHVVITLLRE VVFTRLVQRC WQWGNDRKSI 

       370        380        390        400        410        420 
MPLKILLATK QHDSSANEDE EEQLDALECR LASAIASGLL RAYLSHSNRC IVFSKKEPFP 


HSK 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"COP9 signalosome components play a role in the mating pheromone response of S. cerevisiae."
Maytal-Kivity V., Piran R., Pick E., Hofmann K., Glickman M.H.
EMBO Rep. 3:1215-1221(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RRI1; CSN9; RRI2 AND CSI1, IDENTIFICATION IN THE COP9 SIGNALOSOME COMPLEX, FUNCTION OF THE COP9 SIGNALOSOME COMPLEX.
[5]"The COP9 signalosome-like complex in S. cerevisiae and links to other PCI complexes."
Maytal-Kivity V., Pick E., Piran R., Hofmann K., Glickman M.H.
Int. J. Biochem. Cell Biol. 35:706-715(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH RRI1/CSN5; CSN9; RRI2/CSN10 AND CSI1, IDENTIFICATION IN THE COP9 SIGNALOSOME COMPLEX.
[6]"A genetic interaction map of RNA-processing factors reveals links between Sem1/Dss1-containing complexes and mRNA export and splicing."
Wilmes G.M., Bergkessel M., Bandyopadhyay S., Shales M., Braberg H., Cagney G., Collins S.R., Whitworth G.B., Kress T.L., Weissman J.S., Ideker T., Guthrie C., Krogan N.J.
Mol. Cell 32:735-746(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SEM1 AND THP3.
[7]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]"Sem1 is a functional component of the nuclear pore complex-associated messenger RNA export machinery."
Faza M.B., Kemmler S., Jimeno S., Gonzalez-Aguilera C., Aguilera A., Hurt E., Panse V.G.
J. Cell Biol. 184:833-846(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH SEM1 AND THP3.
[9]"New suppressors of THO mutations identify Thp3 (Ypr045c)-Csn12 as a protein complex involved in transcription elongation."
Jimeno S., Tous C., Garcia-Rubio M.L., Ranes M., Gonzalez-Aguilera C., Marin A., Aguilera A.
Mol. Cell. Biol. 31:674-685(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INTERACTION WITH THP3.
[10]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49584 Genomic DNA. Translation: CAA89611.1.
L47993 Genomic DNA. Translation: AAB39307.1.
BK006943 Genomic DNA. Translation: DAA08869.1.
PIRS57103.
RefSeqNP_012617.1. NM_001181741.1.

3D structure databases

ProteinModelPortalP47130.
SMRP47130. Positions 209-420.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33839. 61 interactions.
DIPDIP-6710N.
IntActP47130. 9 interactions.
MINTMINT-655129.
STRING4932.YJR084W.

Proteomic databases

PaxDbP47130.
PeptideAtlasP47130.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR084W; YJR084W; YJR084W.
GeneID853546.
KEGGsce:YJR084W.

Organism-specific databases

CYGDYJR084w.
SGDS000003844. CSN12.

Phylogenomic databases

eggNOGCOG5600.
GeneTreeENSGT00390000001101.
HOGENOMHOG000000818.
OMARNDIYFF.
OrthoDBEOG712V5T.

Enzyme and pathway databases

BioCycYEAST:G3O-31712-MONOMER.

Gene expression databases

GenevestigatorP47130.

Family and domain databases

InterProIPR013143. PAM.
IPR000717. PCI_dom.
[Graphical view]
PfamPF01399. PCI. 1 hit.
[Graphical view]
SMARTSM00753. PAM. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974272.
PROP47130.

Entry information

Entry nameCSN12_YEAST
AccessionPrimary (citable) accession number: P47130
Secondary accession number(s): D6VWQ3
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families