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Protein

Assembly-complementing factor 4

Gene

ACF4

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be involved in actin cytoskeleton organization and biogenesis.1 Publication

Enzyme and pathway databases

BioCyciYEAST:G3O-31711-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Assembly-complementing factor 4
Gene namesi
Name:ACF4
Ordered Locus Names:YJR083C
ORF Names:J1857
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR083c.
EuPathDBiFungiDB:YJR083C.
SGDiS000003843. ACF4.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 309309Assembly-complementing factor 4PRO_0000203104Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei44 – 441Phosphoserine1 Publication
Modified residuei71 – 711Phosphoserine2 Publications
Modified residuei74 – 741Phosphoserine3 Publications
Modified residuei78 – 781Phosphoserine2 Publications
Modified residuei165 – 1651Phosphoserine3 Publications
Modified residuei288 – 2881Phosphoserine3 Publications

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47129.
PaxDbiP47129.

Expressioni

Gene expression databases

GenevestigatoriP47129.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
LSB3P436034EBI-25556,EBI-22980
RVS167P397435EBI-25556,EBI-14500
YSC84P327934EBI-25556,EBI-24460

Protein-protein interaction databases

BioGridi33838. 23 interactions.
DIPiDIP-2890N.
IntActiP47129. 8 interactions.
MINTiMINT-399509.
STRINGi4932.YJR083C.

Structurei

3D structure databases

ProteinModelPortaliP47129.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Phylogenomic databases

eggNOGiNOG68626.
InParanoidiP47129.
OMAiKHREINE.
OrthoDBiEOG7P2Z44.

Sequencei

Sequence statusi: Complete.

P47129-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEDQRVISQ PIELHKLSIV DKHSQGQQQQ PHQKQHEVQP ESKSPRVTTP
60 70 80 90 100
LKPKRLAIPI SSPQRSTTNQ SPVSDHASPI STDQDLIYKL AAKHREINEL
110 120 130 140 150
SFKLEVAQKE LKQLELQFKD TLPRNGQQKL GNQNPSEYLS TFTKRIQQTF
160 170 180 190 200
VDVNNSPNML KGKKSINDFF SKPNNNVNSN INNTLPNRKP NPPPNRSQRM
210 220 230 240 250
QNIAPSRSSE STPTSGPPLL PPRNTMKNAN TTATAGENTP FLQRILNKFN
260 270 280 290 300
QMNMEEDEFD DLLEKRKSKK DHYYIKENLG YEYDEVRSED EDDEEFEPMG

DIPVHLFKR
Length:309
Mass (Da):35,521
Last modified:February 1, 1996 - v1
Checksum:iFE29E8D0192B2113
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49583 Genomic DNA. Translation: CAA89610.1.
L47993 Genomic DNA. Translation: AAB39306.1.
AY557912 Genomic DNA. Translation: AAS56238.1.
BK006943 Genomic DNA. Translation: DAA08868.1.
PIRiS57102.
RefSeqiNP_012616.1. NM_001181740.1.

Genome annotation databases

EnsemblFungiiYJR083C; YJR083C; YJR083C.
GeneIDi853545.
KEGGisce:YJR083C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49583 Genomic DNA. Translation: CAA89610.1.
L47993 Genomic DNA. Translation: AAB39306.1.
AY557912 Genomic DNA. Translation: AAS56238.1.
BK006943 Genomic DNA. Translation: DAA08868.1.
PIRiS57102.
RefSeqiNP_012616.1. NM_001181740.1.

3D structure databases

ProteinModelPortaliP47129.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33838. 23 interactions.
DIPiDIP-2890N.
IntActiP47129. 8 interactions.
MINTiMINT-399509.
STRINGi4932.YJR083C.

Proteomic databases

MaxQBiP47129.
PaxDbiP47129.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR083C; YJR083C; YJR083C.
GeneIDi853545.
KEGGisce:YJR083C.

Organism-specific databases

CYGDiYJR083c.
EuPathDBiFungiDB:YJR083C.
SGDiS000003843. ACF4.

Phylogenomic databases

eggNOGiNOG68626.
InParanoidiP47129.
OMAiKHREINE.
OrthoDBiEOG7P2Z44.

Enzyme and pathway databases

BioCyciYEAST:G3O-31711-MONOMER.

Miscellaneous databases

NextBioi974269.
PROiP47129.

Gene expression databases

GenevestigatoriP47129.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "Predicting protein functions from redundancies in large-scale protein interaction networks."
    Samanta M.P., Liang S.
    Proc. Natl. Acad. Sci. U.S.A. 100:12579-12583(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION PREDICTION.
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; SER-74; SER-78; SER-165 AND SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; SER-74; SER-165 AND SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-74; SER-78; SER-165 AND SER-288, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiACF4_YEAST
AccessioniPrimary (citable) accession number: P47129
Secondary accession number(s): D6VWQ2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 29, 2015
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1140 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.