Reviewed, UniProtKB/Swiss-Prot P47129 (ACF4_YEAST)

Names and origin

Protein names

Recommended name:
    Assembly-complementing factor 4

Gene names

Name:	ACF4
Ordered Locus Names:	YJR083C
ORF Names:	J1857

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	309 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	May be involved in actin cytoskeleton organization and biogenesis. Ref.5
Miscellaneous	Present with 1140 molecules/cell in log phase SD medium. Ref.4

Ontologies

Keywords
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ATP14	Q12349	1	EBI-25556,EBI-3071
HSP82	P02829	1	EBI-25556,EBI-8659
LSB3	P43603	2	EBI-25556,EBI-22980
RVS167	P39743	1	EBI-25556,EBI-14500
YSC84	P32793	2	EBI-25556,EBI-24460

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 309	309	Assembly-complementing factor 4		PRO_0000203104
Amino acid modifications
	Modified residue	44	1	Phosphoserine Ref.7 Ref.8
	Modified residue	49	1	Phosphothreonine Ref.7 Ref.8 Ref.9
	Modified residue	61	1	Phosphoserine Ref.8 Ref.9
	Modified residue	62	1	Phosphoserine Ref.8 Ref.9
	Modified residue	71	1	Phosphoserine Ref.8 Ref.9 Ref.6
	Modified residue	74	1	Phosphoserine Ref.8 Ref.9 Ref.6
	Modified residue	78	1	Phosphoserine Ref.8 Ref.9 Ref.6
	Modified residue	165	1	Phosphoserine Ref.8 Ref.9 Ref.6
	Modified residue	288	1	Phosphoserine Ref.9 Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P47129-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: FE29E8D0192B2113	FASTA	309	35,521
	10 20 30 40 50 60 MSEDQRVISQ PIELHKLSIV DKHSQGQQQQ PHQKQHEVQP ESKSPRVTTP LKPKRLAIPI 70 80 90 100 110 120 SSPQRSTTNQ SPVSDHASPI STDQDLIYKL AAKHREINEL SFKLEVAQKE LKQLELQFKD 130 140 150 160 170 180 TLPRNGQQKL GNQNPSEYLS TFTKRIQQTF VDVNNSPNML KGKKSINDFF SKPNNNVNSN 190 200 210 220 230 240 INNTLPNRKP NPPPNRSQRM QNIAPSRSSE STPTSGPPLL PPRNTMKNAN TTATAGENTP 250 260 270 280 290 300 FLQRILNKFN QMNMEEDEFD DLLEKRKSKK DHYYIKENLG YEYDEVRSED EDDEEFEPMG DIPVHLFKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed: 8840504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[5]	"Predicting protein functions from redundancies in large-scale protein interaction networks." Samanta M.P., Liang S. Proc. Natl. Acad. Sci. U.S.A. 100:12579-12583(2003) [PubMed: 14566057] [Abstract] Cited for: FUNCTION PREDICTION.
[6]	"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae." Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P. J. Proteome Res. 6:1190-1197(2007) [PubMed: 17330950] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-71; SER-74; SER-78; SER-165 AND SER-288, MASS SPECTROMETRY.
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44 AND THR-49, MASS SPECTROMETRY.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-44; THR-49; SER-61; SER-62; SER-71; SER-74; SER-78 AND SER-165, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-49; SER-61; SER-62; SER-71; SER-74; SER-78; SER-165 AND SER-288, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z49583 Genomic DNA. Translation: CAA89610.1. L47993 Genomic DNA. Translation: AAB39306.1. AY557912 Genomic DNA. Translation: AAS56238.1.
PIR	S57102.
RefSeq	NP_012616.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2890N.
IntAct	P47129. 11 interactions.
STRING	P47129.
Genome annotation databases
Ensembl	YJR083C; YJR083C; YJR083C; Saccharomyces cerevisiae. [Genome view]
GeneID	853545.
KEGG	sce:YJR083C.
NMPDR	fig|4932.3.peg.3590.
Organism-specific databases
CYGD	YJR083c.
SGD	S000003843. ACF4.
Phylogenomic databases
HOGENOM	P47129.
OMA	SNAGNSN
OrthoDB	EOG9Q5B6T
Gene expression databases
ArrayExpress	P47129.
Genevestigator	P47129.
GermOnline	YJR083C. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other Resources
NextBio	974269.

Entry information

Entry name

ACF4_YEAST

Accession

Primary (citable) accession number: P47129

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)