Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

GPN-loop GTPase 1

Gene

NPA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed:20855544, PubMed:21844196). May act at an RNAP assembly step prior to nuclear import (PubMed:23267056). Promotes sister chromatid separation during anaphase (PubMed:21532343).1 Publication4 Publications

Miscellaneous

Present with 15200 molecules/cell in log phase SD medium.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei72Stabilizes the phosphate intermediate; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 18GTPBy similarity6
Nucleotide bindingi173 – 176GTPBy similarity4

GO - Molecular functioni

  • ATPase activity Source: SGD
  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • mitotic sister chromatid cohesion Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • protein import into nucleus Source: SGD

Keywordsi

Molecular functionHydrolase
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31704-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
GPN-loop GTPase 11 Publication (EC:3.6.5.-1 Publication)
Alternative name(s):
Essential PCL1-interacting ATPase 11 Publication
GPN-loop GTPase NPA3Curated
Nucleolar preribosomal-associated protein 31 Publication
Gene namesi
Name:NPA31 Publication
Synonyms:EPA11 Publication, GPN11 Publication
Ordered Locus Names:YJR072CImported
ORF Names:J1821
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR072C
SGDiS000003833 NPA3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106D → A: Impairs nuclear localization of RNAPII. Completely abolishes RNAPII binding. 1 Publication1
Mutagenesisi110Q → L: Impairs nuclear localization of RNAPII, but does not impair RNAPII binding. 1 Publication1
Mutagenesisi112E → K: Impairs heterodimer formation with GPN2 and GPN3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002031011 – 385GPN-loop GTPase 1Add BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei304PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei352PhosphoserineCombined sources1
Cross-linki369Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

Phosphorylated by the cyclin-CDK PCL1-PHO85.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP47122
PaxDbiP47122
PRIDEiP47122

PTM databases

iPTMnetiP47122

Interactioni

Subunit structurei

Heterodimers with GPN2 or GPN3 (PubMed:23324351, PubMed:23267056). Binds to RNA polymerase II (RNAPII) in a GTP-dependent manner (PubMed:20855544, PubMed:21844196). Interacts with nuclear pore protein NUP133 and nuclear export factor CRM1 (PubMed:21844196). Interacts with PCL1 (PubMed:15082539).5 Publications

Protein-protein interaction databases

BioGridi33828, 81 interactors
DIPiDIP-1676N
IntActiP47122, 75 interactors
MINTiP47122
STRINGi4932.YJR072C

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi16 – 29Combined sources14
Beta strandi35 – 38Combined sources4
Beta strandi51 – 54Combined sources4
Turni55 – 57Combined sources3
Helixi60 – 67Combined sources8
Helixi73 – 84Combined sources12
Helixi86 – 95Combined sources10
Turni96 – 99Combined sources4
Beta strandi101 – 106Combined sources6
Helixi112 – 115Combined sources4
Helixi118 – 128Combined sources11
Beta strandi133 – 139Combined sources7
Helixi141 – 143Combined sources3
Helixi147 – 164Combined sources18
Beta strandi168 – 172Combined sources5
Helixi175 – 177Combined sources3
Helixi182 – 197Combined sources16
Helixi216 – 231Combined sources16
Beta strandi232 – 236Combined sources5
Turni239 – 241Combined sources3
Helixi245 – 262Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HCIX-ray2.30A/B/C/D/E/F2-264[»]
5HCNX-ray2.20A2-264[»]
ProteinModelPortaliP47122
SMRiP47122
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi70 – 72Gly-Pro-Asn (GPN)-loop; involved in dimer interfaceBy similarity3

Sequence similaritiesi

Belongs to the GPN-loop GTPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074926
HOGENOMiHOG000191633
InParanoidiP47122
KOiK06883
OMAiESMAQYW
OrthoDBiEOG092C47W3

Family and domain databases

InterProiView protein in InterPro
IPR004130 Gpn
IPR030230 Gpn1/Npa3/XAB1
IPR027417 P-loop_NTPase
PANTHERiPTHR21231 PTHR21231, 1 hit
PTHR21231:SF8 PTHR21231:SF8, 1 hit
PfamiView protein in Pfam
PF03029 ATP_bind_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequencei

Sequence statusi: Complete.

P47122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA
60 70 80 90 100
NIDIRDSIKY KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF
110 120 130 140 150
QNCIIDTPGQ IECFVWSASG AIITESFASS FPTVIAYIVD TPRNSSPTTF
160 170 180 190 200
MSNMLYACSI LYKTKLPMIV VFNKTDVCKA DFAKEWMTDF ESFQAAIKED
210 220 230 240 250
QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF TGDGFDEFMQ
260 270 280 290 300
CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS
310 320 330 340 350
AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE
360 370 380
NSAPDLQRRY QEAMQQVGKT ASSETAENIA KYIRN
Length:385
Mass (Da):43,195
Last modified:February 1, 1996 - v1
Checksum:iEA271D14EA1FFAEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49572 Genomic DNA Translation: CAA89600.1
L47993 Genomic DNA Translation: AAB39297.1
BK006943 Genomic DNA Translation: DAA08858.1
PIRiS57091
RefSeqiNP_012606.3, NM_001181730.3

Genome annotation databases

EnsemblFungiiYJR072C; YJR072C; YJR072C
GeneIDi853535
KEGGisce:YJR072C

Similar proteinsi

Entry informationi

Entry nameiGPN1_YEAST
AccessioniPrimary (citable) accession number: P47122
Secondary accession number(s): D6VWP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health