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Protein

GPN-loop GTPase 1

Gene

NPA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed:20855544, PubMed:21844196). May act at an RNAP assembly step prior to nuclear import (PubMed:23267056). Promotes sister chromatid separation during anaphase (PubMed:21532343).1 Publication4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei72Stabilizes the phosphate intermediate; shared with dimeric partnerBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi13 – 18GTPBy similarity6
Nucleotide bindingi173 – 176GTPBy similarity4

GO - Molecular functioni

  • ATPase activity Source: SGD
  • GTPase activity Source: SGD
  • GTP binding Source: UniProtKB-KW

GO - Biological processi

  • mitotic sister chromatid cohesion Source: SGD
  • nucleocytoplasmic transport Source: SGD
  • RNA polymerase II complex import to nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31704-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GPN-loop GTPase 11 Publication (EC:3.6.5.-1 Publication)
Alternative name(s):
Essential PCL1-interacting ATPase 11 Publication
GPN-loop GTPase NPA3Curated
Nucleolar preribosomal-associated protein 31 Publication
Gene namesi
Name:NPA31 Publication
Synonyms:EPA11 Publication, GPN11 Publication
Ordered Locus Names:YJR072CImported
ORF Names:J1821
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR072C.
SGDiS000003833. NPA3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi106D → A: Impairs nuclear localization of RNAPII. Completely abolishes RNAPII binding. 1 Publication1
Mutagenesisi110Q → L: Impairs nuclear localization of RNAPII, but does not impair RNAPII binding. 1 Publication1
Mutagenesisi112E → K: Impairs heterodimer formation with GPN2 and GPN3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002031011 – 385GPN-loop GTPase 1Add BLAST385

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei304PhosphoserineCombined sources1
Modified residuei308PhosphoserineCombined sources1
Modified residuei313PhosphoserineCombined sources1
Modified residuei352PhosphoserineCombined sources1
Cross-linki369Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)Combined sources

Post-translational modificationi

Phosphorylated by the cyclin-CDK PCL1-PHO85.1 Publication

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiP47122.
PRIDEiP47122.

PTM databases

iPTMnetiP47122.

Interactioni

Subunit structurei

Heterodimers with GPN2 or GPN3 (PubMed:23324351, PubMed:23267056). Binds to RNA polymerase II (RNAPII) in a GTP-dependent manner (PubMed:20855544, PubMed:21844196). Interacts with nuclear pore protein NUP133 and nuclear export factor CRM1 (PubMed:21844196). Interacts with PCL1 (PubMed:15082539).5 Publications

Protein-protein interaction databases

BioGridi33828. 64 interactors.
DIPiDIP-1676N.
IntActiP47122. 66 interactors.
MINTiMINT-395463.

Structurei

Secondary structure

1385
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 9Combined sources6
Helixi16 – 29Combined sources14
Beta strandi35 – 38Combined sources4
Beta strandi51 – 54Combined sources4
Turni55 – 57Combined sources3
Helixi60 – 67Combined sources8
Helixi73 – 84Combined sources12
Helixi86 – 95Combined sources10
Turni96 – 99Combined sources4
Beta strandi101 – 106Combined sources6
Helixi112 – 115Combined sources4
Helixi118 – 128Combined sources11
Beta strandi133 – 139Combined sources7
Helixi141 – 143Combined sources3
Helixi147 – 164Combined sources18
Beta strandi168 – 172Combined sources5
Helixi175 – 177Combined sources3
Helixi182 – 197Combined sources16
Helixi216 – 231Combined sources16
Beta strandi232 – 236Combined sources5
Turni239 – 241Combined sources3
Helixi245 – 262Combined sources18

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HCIX-ray2.30A/B/C/D/E/F2-264[»]
5HCNX-ray2.20A2-264[»]
ProteinModelPortaliP47122.
SMRiP47122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi70 – 72Gly-Pro-Asn (GPN)-loop; involved in dimer interfaceBy similarity3

Sequence similaritiesi

Belongs to the GPN-loop GTPase family.Curated

Phylogenomic databases

GeneTreeiENSGT00550000074926.
HOGENOMiHOG000191633.
InParanoidiP47122.
KOiK06883.
OMAiKPVCLIV.
OrthoDBiEOG092C47W3.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR004130. Gpn.
IPR030230. Gpn1/Npa3/XAB1.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR21231. PTHR21231. 1 hit.
PTHR21231:SF8. PTHR21231:SF8. 1 hit.
PfamiPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P47122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA
60 70 80 90 100
NIDIRDSIKY KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF
110 120 130 140 150
QNCIIDTPGQ IECFVWSASG AIITESFASS FPTVIAYIVD TPRNSSPTTF
160 170 180 190 200
MSNMLYACSI LYKTKLPMIV VFNKTDVCKA DFAKEWMTDF ESFQAAIKED
210 220 230 240 250
QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF TGDGFDEFMQ
260 270 280 290 300
CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS
310 320 330 340 350
AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE
360 370 380
NSAPDLQRRY QEAMQQVGKT ASSETAENIA KYIRN
Length:385
Mass (Da):43,195
Last modified:February 1, 1996 - v1
Checksum:iEA271D14EA1FFAEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49572 Genomic DNA. Translation: CAA89600.1.
L47993 Genomic DNA. Translation: AAB39297.1.
BK006943 Genomic DNA. Translation: DAA08858.1.
PIRiS57091.
RefSeqiNP_012606.3. NM_001181730.3.

Genome annotation databases

EnsemblFungiiYJR072C; YJR072C; YJR072C.
GeneIDi853535.
KEGGisce:YJR072C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49572 Genomic DNA. Translation: CAA89600.1.
L47993 Genomic DNA. Translation: AAB39297.1.
BK006943 Genomic DNA. Translation: DAA08858.1.
PIRiS57091.
RefSeqiNP_012606.3. NM_001181730.3.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5HCIX-ray2.30A/B/C/D/E/F2-264[»]
5HCNX-ray2.20A2-264[»]
ProteinModelPortaliP47122.
SMRiP47122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33828. 64 interactors.
DIPiDIP-1676N.
IntActiP47122. 66 interactors.
MINTiMINT-395463.

PTM databases

iPTMnetiP47122.

Proteomic databases

MaxQBiP47122.
PRIDEiP47122.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR072C; YJR072C; YJR072C.
GeneIDi853535.
KEGGisce:YJR072C.

Organism-specific databases

EuPathDBiFungiDB:YJR072C.
SGDiS000003833. NPA3.

Phylogenomic databases

GeneTreeiENSGT00550000074926.
HOGENOMiHOG000191633.
InParanoidiP47122.
KOiK06883.
OMAiKPVCLIV.
OrthoDBiEOG092C47W3.

Enzyme and pathway databases

BioCyciYEAST:G3O-31704-MONOMER.

Miscellaneous databases

PROiP47122.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR004130. Gpn.
IPR030230. Gpn1/Npa3/XAB1.
IPR027417. P-loop_NTPase.
[Graphical view]
PANTHERiPTHR21231. PTHR21231. 1 hit.
PTHR21231:SF8. PTHR21231:SF8. 1 hit.
PfamiPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGPN1_YEAST
AccessioniPrimary (citable) accession number: P47122
Secondary accession number(s): D6VWP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 133 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.