Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P47122

- GPN1_YEAST

UniProt

P47122 - GPN1_YEAST

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

GPN-loop GTPase 1

Gene

NPA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Small GTPase required for proper nuclear import of RNA polymerase II (RNAPII) (PubMed:20855544, PubMed:21844196). May act at an RNAP assembly step prior to nuclear import (PubMed:23267056). Promotes sister chromatid separation during anaphase (PubMed:21532343).4 Publications1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei72 – 721Stabilizes the phosphate intermediate; shared with dimeric partnerBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 186GTPBy similarity
Nucleotide bindingi173 – 1764GTPBy similarity

GO - Molecular functioni

  1. ATPase activity Source: SGD
  2. GTPase activity Source: SGD
  3. GTP binding Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. GTP catabolic process Source: GOC
  3. mitotic sister chromatid cohesion Source: SGD
  4. nucleocytoplasmic transport Source: SGD
  5. RNA polymerase II complex localization to nucleus Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31704-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
GPN-loop GTPase 11 Publication (EC:3.6.5.-1 Publication)
Alternative name(s):
Essential PCL1-interacting ATPase 11 Publication
GPN-loop GTPase NPA3Curated
Nucleolar preribosomal-associated protein 31 Publication
Gene namesi
Name:NPA31 Publication
Synonyms:EPA11 Publication, GPN11 Publication
Ordered Locus Names:YJR072CImported
ORF Names:J1821
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR072c.
SGDiS000003833. NPA3.

Subcellular locationi

Cytoplasm 2 Publications

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi106 – 1061D → A: Impairs nuclear localization of RNAPII. Completely abolishes RNAPII binding. 1 Publication
Mutagenesisi110 – 1101Q → L: Impairs nuclear localization of RNAPII, but does not impair RNAPII binding. 1 Publication
Mutagenesisi112 – 1121E → K: Impairs heterodimer formation with GPN2 and GPN3. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 385385GPN-loop GTPase 1PRO_0000203101Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei304 – 3041Phosphoserine1 Publication
Modified residuei308 – 3081Phosphoserine1 Publication
Modified residuei313 – 3131Phosphoserine2 Publications
Modified residuei352 – 3521Phosphoserine2 Publications

Post-translational modificationi

Phosphorylated by the cyclin-CDK PCL1-PHO85.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47122.
PaxDbiP47122.
PeptideAtlasiP47122.

Expressioni

Gene expression databases

GenevestigatoriP47122.

Interactioni

Subunit structurei

Heterodimers with GPN2 or GPN3 (PubMed:23324351, PubMed:23267056). Binds to RNA polymerase II (RNAPII) in a GTP-dependent manner (PubMed:20855544, PubMed:21844196). Interacts with nuclear pore protein NUP133 and nuclear export factor CRM1 (PubMed:21844196). Interacts with PCL1 (PubMed:15082539).5 Publications

Protein-protein interaction databases

BioGridi33828. 64 interactions.
DIPiDIP-1676N.
IntActiP47122. 65 interactions.
MINTiMINT-395463.
STRINGi4932.YJR072C.

Structurei

3D structure databases

ProteinModelPortaliP47122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi70 – 723Gly-Pro-Asn (GPN)-loop; involved in dimer interfaceBy similarity

Sequence similaritiesi

Belongs to the GPN-loop GTPase family.Curated

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00550000074926.
HOGENOMiHOG000191633.
InParanoidiP47122.
KOiK06883.
OMAiKPVCLIV.
OrthoDBiEOG76MKK1.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR004130. Uncharacterised_ATP-bd.
[Graphical view]
PfamiPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.

Sequencei

Sequence statusi: Complete.

P47122-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA
60 70 80 90 100
NIDIRDSIKY KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF
110 120 130 140 150
QNCIIDTPGQ IECFVWSASG AIITESFASS FPTVIAYIVD TPRNSSPTTF
160 170 180 190 200
MSNMLYACSI LYKTKLPMIV VFNKTDVCKA DFAKEWMTDF ESFQAAIKED
210 220 230 240 250
QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF TGDGFDEFMQ
260 270 280 290 300
CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS
310 320 330 340 350
AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE
360 370 380
NSAPDLQRRY QEAMQQVGKT ASSETAENIA KYIRN
Length:385
Mass (Da):43,195
Last modified:February 1, 1996 - v1
Checksum:iEA271D14EA1FFAEF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49572 Genomic DNA. Translation: CAA89600.1.
L47993 Genomic DNA. Translation: AAB39297.1.
BK006943 Genomic DNA. Translation: DAA08858.1.
PIRiS57091.
RefSeqiNP_012606.3. NM_001181730.3.

Genome annotation databases

EnsemblFungiiYJR072C; YJR072C; YJR072C.
GeneIDi853535.
KEGGisce:YJR072C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49572 Genomic DNA. Translation: CAA89600.1 .
L47993 Genomic DNA. Translation: AAB39297.1 .
BK006943 Genomic DNA. Translation: DAA08858.1 .
PIRi S57091.
RefSeqi NP_012606.3. NM_001181730.3.

3D structure databases

ProteinModelPortali P47122.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33828. 64 interactions.
DIPi DIP-1676N.
IntActi P47122. 65 interactions.
MINTi MINT-395463.
STRINGi 4932.YJR072C.

Proteomic databases

MaxQBi P47122.
PaxDbi P47122.
PeptideAtlasi P47122.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR072C ; YJR072C ; YJR072C .
GeneIDi 853535.
KEGGi sce:YJR072C.

Organism-specific databases

CYGDi YJR072c.
SGDi S000003833. NPA3.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00550000074926.
HOGENOMi HOG000191633.
InParanoidi P47122.
KOi K06883.
OMAi KPVCLIV.
OrthoDBi EOG76MKK1.

Enzyme and pathway databases

BioCyci YEAST:G3O-31704-MONOMER.

Miscellaneous databases

NextBioi 974239.
PROi P47122.

Gene expression databases

Genevestigatori P47122.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR004130. Uncharacterised_ATP-bd.
[Graphical view ]
Pfami PF03029. ATP_bind_1. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  6. "The identification of Pcl1-interacting proteins that genetically interact with Cla4 may indicate a link between G1 progression and mitotic exit."
    Keniry M.E., Kemp H.A., Rivers D.M., Sprague G.F. Jr.
    Genetics 166:1177-1186(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH PCL1.
  7. "Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification."
    Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y.
    Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313 AND SER-352, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304; SER-308; SER-313 AND SER-352, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  10. "The protein interaction network of the human transcription machinery reveals a role for the conserved GTPase RPAP4/GPN1 and microtubule assembly in nuclear import and biogenesis of RNA polymerase II."
    Forget D., Lacombe A.A., Cloutier P., Al-Khoury R., Bouchard A., Lavallee-Adam M., Faubert D., Jeronimo C., Blanchette M., Coulombe B.
    Mol. Cell. Proteomics 9:2827-2839(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BINDING TO RNA POYMERASE II.
  11. "A role for GPN-loop GTPase yGPN1 in sister chromatid cohesion."
    Alonso B., Chaussinand G., Armengaud J., Godon C.
    Cell Cycle 10:1828-1837(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  12. "GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein."
    Staresincic L., Walker J., Dirac-Svejstrup A.B., Mitter R., Svejstrup J.Q.
    J. Biol. Chem. 286:35553-35561(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, BINDING TO RNA POYMERASE II, INTERACTION WITH NUP133 AND CRM1, MUTAGENESIS OF ASP-106 AND GLN-110.
  13. "Eukaryotic GPN-loop GTPases paralogs use a dimeric assembly reminiscent of archeal GPN."
    Alonso B., Beraud C., Meguellati S., Chen S.W., Pellequer J.L., Armengaud J., Godon C.
    Cell Cycle 12:463-472(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GPN2 AND GPN3, MUTAGENESIS OF GLU-112.
  14. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
    Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
    Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Biogenesis of RNA polymerases II and III requires the conserved GPN small GTPases in Saccharomyces cerevisiae."
    Minaker S.W., Filiatrault M.C., Ben-Aroya S., Hieter P., Stirling P.C.
    Genetics 193:853-864(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH GPN2.

Entry informationi

Entry nameiGPN1_YEAST
AccessioniPrimary (citable) accession number: P47122
Secondary accession number(s): D6VWP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 26, 2014
This is version 114 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 15200 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3