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P47122 (NPA3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
GTPase NPA3

EC=3.6.-.-
Alternative name(s):
Essential PCL1-interacting ATPase 1
Gene names
Name:NPA3
Synonyms:EPA1
Ordered Locus Names:YJR072C
ORF Names:J1821
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length385 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

GTPase essential for viability. May have a role in mitotic exit. Ref.6

Subunit structure

Interacts with PCL1. Ref.6

Subcellular location

Cytoplasm Ref.4 Ref.7.

Post-translational modification

Phosphorylated by the cyclin-CDK PCL1-PHO85. Ref.6

Miscellaneous

Present with 15200 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the GPN-loop GTPase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 385385GTPase NPA3
PRO_0000203101

Regions

Nucleotide binding10 – 178GTP (Pootential)

Amino acid modifications

Modified residue3041Phosphoserine Ref.9
Modified residue3081Phosphoserine Ref.9
Modified residue3131Phosphoserine Ref.8 Ref.9
Modified residue3521Phosphoserine Ref.8 Ref.9

Sequences

Sequence LengthMass (Da)Tools
P47122 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: EA271D14EA1FFAEF

FASTA38543,195
        10         20         30         40         50         60 
MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA NIDIRDSIKY 

        70         80         90        100        110        120 
KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF QNCIIDTPGQ IECFVWSASG 

       130        140        150        160        170        180 
AIITESFASS FPTVIAYIVD TPRNSSPTTF MSNMLYACSI LYKTKLPMIV VFNKTDVCKA 

       190        200        210        220        230        240 
DFAKEWMTDF ESFQAAIKED QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF 

       250        260        270        280        290        300 
TGDGFDEFMQ CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS 

       310        320        330        340        350        360 
AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE NSAPDLQRRY 

       370        380 
QEAMQQVGKT ASSETAENIA KYIRN 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Global analysis of protein localization in budding yeast."
Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K.
Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[5]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]"The identification of Pcl1-interacting proteins that genetically interact with Cla4 may indicate a link between G1 progression and mitotic exit."
Keniry M.E., Kemp H.A., Rivers D.M., Sprague G.F. Jr.
Genetics 166:1177-1186(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH PCL1.
[7]"Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification."
Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y.
Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313 AND SER-352, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304; SER-308; SER-313 AND SER-352, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[10]"Sites of ubiquitin attachment in Saccharomyces cerevisiae."
Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
Z49572 Genomic DNA. Translation: CAA89600.1.
L47993 Genomic DNA. Translation: AAB39297.1.
BK006943 Genomic DNA. Translation: DAA08858.1.
PIRS57091.
RefSeqNP_012606.3. NM_001181730.3.

3D structure databases

ProteinModelPortalP47122.
SMRP47122. Positions 1-255.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33828. 64 interactions.
DIPDIP-1676N.
IntActP47122. 65 interactions.
MINTMINT-395463.
STRING4932.YJR072C.

Proteomic databases

PaxDbP47122.
PeptideAtlasP47122.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR072C; YJR072C; YJR072C.
GeneID853535.
KEGGsce:YJR072C.

Organism-specific databases

CYGDYJR072c.
SGDS000003833. NPA3.

Phylogenomic databases

eggNOGCOG1100.
GeneTreeENSGT00550000074926.
HOGENOMHOG000191633.
KOK06883.
OMAKPVCLIV.
OrthoDBEOG76MKK1.

Enzyme and pathway databases

BioCycYEAST:G3O-31704-MONOMER.

Gene expression databases

GenevestigatorP47122.

Family and domain databases

Gene3D3.40.50.300. 1 hit.
InterProIPR027417. P-loop_NTPase.
IPR004130. Uncharacterised_ATP-bd.
[Graphical view]
PfamPF03029. ATP_bind_1. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 1 hit.
ProtoNetSearch...

Other

NextBio974239.
PROP47122.

Entry information

Entry nameNPA3_YEAST
AccessionPrimary (citable) accession number: P47122
Secondary accession number(s): D6VWP2
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families