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P47122

- NPA3_YEAST

UniProt

P47122 - NPA3_YEAST

Protein

GTPase NPA3

Gene

NPA3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 112 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    GTPase essential for viability. May have a role in mitotic exit.1 Publication

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi10 – 178GTP (Pootential)

    GO - Molecular functioni

    1. ATPase activity Source: SGD
    2. GTPase activity Source: SGD
    3. GTP binding Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. GTP catabolic process Source: GOC
    3. mitotic sister chromatid cohesion Source: SGD
    4. nucleocytoplasmic transport Source: SGD
    5. RNA polymerase II complex localization to nucleus Source: SGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    GTP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31704-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    GTPase NPA3 (EC:3.6.-.-)
    Alternative name(s):
    Essential PCL1-interacting ATPase 1
    Gene namesi
    Name:NPA3
    Synonyms:EPA1
    Ordered Locus Names:YJR072C
    ORF Names:J1821
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR072c.
    SGDiS000003833. NPA3.

    Subcellular locationi

    Cytoplasm 2 Publications

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 385385GTPase NPA3PRO_0000203101Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei304 – 3041Phosphoserine2 Publications
    Modified residuei308 – 3081Phosphoserine2 Publications
    Modified residuei313 – 3131Phosphoserine3 Publications
    Modified residuei352 – 3521Phosphoserine3 Publications

    Post-translational modificationi

    Phosphorylated by the cyclin-CDK PCL1-PHO85.3 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47122.
    PaxDbiP47122.
    PeptideAtlasiP47122.

    Expressioni

    Gene expression databases

    GenevestigatoriP47122.

    Interactioni

    Subunit structurei

    Interacts with PCL1.1 Publication

    Protein-protein interaction databases

    BioGridi33828. 64 interactions.
    DIPiDIP-1676N.
    IntActiP47122. 65 interactions.
    MINTiMINT-395463.
    STRINGi4932.YJR072C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47122.
    SMRiP47122. Positions 3-41.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the GPN-loop GTPase family.Curated

    Phylogenomic databases

    eggNOGiCOG1100.
    GeneTreeiENSGT00550000074926.
    HOGENOMiHOG000191633.
    KOiK06883.
    OMAiKPVCLIV.
    OrthoDBiEOG76MKK1.

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR004130. Uncharacterised_ATP-bd.
    [Graphical view]
    PfamiPF03029. ATP_bind_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P47122-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA    50
    NIDIRDSIKY KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF 100
    QNCIIDTPGQ IECFVWSASG AIITESFASS FPTVIAYIVD TPRNSSPTTF 150
    MSNMLYACSI LYKTKLPMIV VFNKTDVCKA DFAKEWMTDF ESFQAAIKED 200
    QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF TGDGFDEFMQ 250
    CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS 300
    AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE 350
    NSAPDLQRRY QEAMQQVGKT ASSETAENIA KYIRN 385
    Length:385
    Mass (Da):43,195
    Last modified:February 1, 1996 - v1
    Checksum:iEA271D14EA1FFAEF
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49572 Genomic DNA. Translation: CAA89600.1.
    L47993 Genomic DNA. Translation: AAB39297.1.
    BK006943 Genomic DNA. Translation: DAA08858.1.
    PIRiS57091.
    RefSeqiNP_012606.3. NM_001181730.3.

    Genome annotation databases

    EnsemblFungiiYJR072C; YJR072C; YJR072C.
    GeneIDi853535.
    KEGGisce:YJR072C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z49572 Genomic DNA. Translation: CAA89600.1 .
    L47993 Genomic DNA. Translation: AAB39297.1 .
    BK006943 Genomic DNA. Translation: DAA08858.1 .
    PIRi S57091.
    RefSeqi NP_012606.3. NM_001181730.3.

    3D structure databases

    ProteinModelPortali P47122.
    SMRi P47122. Positions 3-41.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33828. 64 interactions.
    DIPi DIP-1676N.
    IntActi P47122. 65 interactions.
    MINTi MINT-395463.
    STRINGi 4932.YJR072C.

    Proteomic databases

    MaxQBi P47122.
    PaxDbi P47122.
    PeptideAtlasi P47122.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR072C ; YJR072C ; YJR072C .
    GeneIDi 853535.
    KEGGi sce:YJR072C.

    Organism-specific databases

    CYGDi YJR072c.
    SGDi S000003833. NPA3.

    Phylogenomic databases

    eggNOGi COG1100.
    GeneTreei ENSGT00550000074926.
    HOGENOMi HOG000191633.
    KOi K06883.
    OMAi KPVCLIV.
    OrthoDBi EOG76MKK1.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31704-MONOMER.

    Miscellaneous databases

    NextBioi 974239.
    PROi P47122.

    Gene expression databases

    Genevestigatori P47122.

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR004130. Uncharacterised_ATP-bd.
    [Graphical view ]
    Pfami PF03029. ATP_bind_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    5. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    6. "The identification of Pcl1-interacting proteins that genetically interact with Cla4 may indicate a link between G1 progression and mitotic exit."
      Keniry M.E., Kemp H.A., Rivers D.M., Sprague G.F. Jr.
      Genetics 166:1177-1186(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, PHOSPHORYLATION, INTERACTION WITH PCL1.
    7. "Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification."
      Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B., Caizergues-Ferrer M., Henry Y.
      Mol. Cell. Biol. 24:6324-6337(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY, SUBCELLULAR LOCATION.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-313 AND SER-352, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-304; SER-308; SER-313 AND SER-352, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Sites of ubiquitin attachment in Saccharomyces cerevisiae."
      Starita L.M., Lo R.S., Eng J.K., von Haller P.D., Fields S.
      Proteomics 12:236-240(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiNPA3_YEAST
    AccessioniPrimary (citable) accession number: P47122
    Secondary accession number(s): D6VWP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 112 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 15200 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3