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Protein

Deoxyhypusine hydroxylase

Gene

LIA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N(6)-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation2 Publications

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation1 Publication

Cofactori

Fe2+UniRule annotation1 PublicationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation1 Publication

pH dependencei

Optimum pH is 7.5. Active from pH 6 to 10.1 Publication

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Iron 11
Metal bindingi80Iron 11
Metal bindingi112Iron 11
Metal bindingi113Iron 11
Metal bindingi237Iron 21
Metal bindingi238Iron 21
Metal bindingi270Iron 21
Metal bindingi271Iron 21

GO - Molecular functioni

  • deoxyhypusine monooxygenase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • microtubule cytoskeleton organization Source: SGD
  • peptidyl-lysine modification to peptidyl-hypusine Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Monooxygenase, Oxidoreductase

Keywords - Biological processi

Hypusine biosynthesis

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31703-MONOMER.
ReactomeiR-SCE-204626. Hypusine synthesis from eIF5A-lysine.
UniPathwayiUPA00354.

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Ligand of eIF5A protein 1UniRule annotation
Gene namesi
Name:LIA1UniRule annotation
Synonyms:MMD1
Ordered Locus Names:YJR070C
ORF Names:J1814
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR070C.
SGDiS000003831. LIA1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi79H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi80E → A: Abolishes enzyme activity and impairs iron-binding. 1 Publication1
Mutagenesisi112H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi113E → A: Abolishes iron-binding, substrate-binding, and enzyme activity. 1 Publication1
Mutagenesisi116E → A: Abolishes substrate-binding and enzyme activity. 1 Publication1
Mutagenesisi116E → D: Reduces enzyme activity by 97%. 1 Publication1
Mutagenesisi237H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi238E → A: Abolishes substrate-binding, enzyme activity, and impairs iron-binding. 1 Publication1
Mutagenesisi270H → A: Abolishes iron-binding, substrate-binding, and enzyme activity. 1 Publication1
Mutagenesisi271E → A: Abolishes iron-binding, substrate-binding, and enzyme activity. 1 Publication1
Mutagenesisi274E → A: Abolishes substrate-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation1 Publication
ChainiPRO_00002031002 – 325Deoxyhypusine hydroxylaseAdd BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineUniRule annotation1 Publication1
Modified residuei126PhosphoserineCombined sources1
Modified residuei187PhosphothreonineCombined sources1
Modified residuei281PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP47120.
PRIDEiP47120.

PTM databases

iPTMnetiP47120.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HYP2P233012EBI-25526,EBI-9033

Protein-protein interaction databases

BioGridi33827. 68 interactors.
DIPiDIP-2829N.
IntActiP47120. 6 interactors.
MINTiMINT-539495.

Structurei

3D structure databases

ProteinModelPortaliP47120.
SMRiP47120.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati77 – 103HEAT-like PBS-type 1Add BLAST27
Repeati110 – 136HEAT-like PBS-type 2Add BLAST27
Repeati202 – 231HEAT-like PBS-type 3Add BLAST30
Repeati235 – 261HEAT-like PBS-type 4Add BLAST27
Repeati268 – 294HEAT-like PBS-type 5Add BLAST27

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation
Contains 5 HEAT-like PBS-type repeats.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
InParanoidiP47120.
KOiK06072.
OMAiGQLCNPV.
OrthoDBiEOG092C3MKB.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNFEKHFQ ENVDECTLEQ LRDILVNKSG KTVLANRFRA LFNLKTVAEE
60 70 80 90 100
FATKPEEAKK AIEYIAESFV NDKSELLKHE VAYVLGQTKN LDAAPTLRHV
110 120 130 140 150
MLDQNQEPMV RHEAAEALGA LGDKDSLDDL NKAAKEDPHV AVRETCELAI
160 170 180 190 200
NRINWTHGGA KDKENLQQSL YSSIDPAPPL PLEKDATIPE LQALLNDPKQ
210 220 230 240 250
PLFQRYRAMF RLRDIGTDEA ILALATGFSA ESSLFKHEIA YVFGQIGSPA
260 270 280 290 300
AVPSLIEVLG RKEEAPMVRH EAAEALGAIA SPEVVDVLKS YLNDEVDVVR
310 320
ESCIVALDMY DYENSNELEY APTAN
Length:325
Mass (Da):36,165
Last modified:February 1, 1996 - v1
Checksum:iAB36A73B40466CD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49570 Genomic DNA. Translation: CAA89598.1.
L47993 Genomic DNA. Translation: AAB39296.1.
BK006943 Genomic DNA. Translation: DAA08857.1.
PIRiS57089.
RefSeqiNP_012604.1. NM_001181728.1.

Genome annotation databases

EnsemblFungiiYJR070C; YJR070C; YJR070C.
GeneIDi853534.
KEGGisce:YJR070C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49570 Genomic DNA. Translation: CAA89598.1.
L47993 Genomic DNA. Translation: AAB39296.1.
BK006943 Genomic DNA. Translation: DAA08857.1.
PIRiS57089.
RefSeqiNP_012604.1. NM_001181728.1.

3D structure databases

ProteinModelPortaliP47120.
SMRiP47120.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33827. 68 interactors.
DIPiDIP-2829N.
IntActiP47120. 6 interactors.
MINTiMINT-539495.

PTM databases

iPTMnetiP47120.

Proteomic databases

MaxQBiP47120.
PRIDEiP47120.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR070C; YJR070C; YJR070C.
GeneIDi853534.
KEGGisce:YJR070C.

Organism-specific databases

EuPathDBiFungiDB:YJR070C.
SGDiS000003831. LIA1.

Phylogenomic databases

GeneTreeiENSGT00500000044957.
HOGENOMiHOG000248665.
InParanoidiP47120.
KOiK06072.
OMAiGQLCNPV.
OrthoDBiEOG092C3MKB.

Enzyme and pathway databases

UniPathwayiUPA00354.
BioCyciYEAST:G3O-31703-MONOMER.
ReactomeiR-SCE-204626. Hypusine synthesis from eIF5A-lysine.

Miscellaneous databases

PROiP47120.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
HAMAPiMF_03101. Deoxyhypusine_hydroxylase. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR027517. Deoxyhypusine_hydroxylase.
IPR004155. PBS_lyase_HEAT.
[Graphical view]
SMARTiSM00567. EZ_HEAT. 5 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiDOHH_YEAST
AccessioniPrimary (citable) accession number: P47120
Secondary accession number(s): D6VWP1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 39900 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.