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Protein

Deoxyhypusine hydroxylase

Gene

LIA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the hydroxylation of the N6-(4-aminobutyl)-L-lysine intermediate to form hypusine, an essential post-translational modification only found in mature eIF-5A factor.UniRule annotation2 Publications

Miscellaneous

Present with 39900 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

[eIF5A]-deoxyhypusine + AH2 + O2 = [eIF5A]-hypusine + A + H2O.UniRule annotation1 Publication

Cofactori

Fe2+UniRule annotation1 PublicationNote: Binds 2 Fe2+ ions per subunit.UniRule annotation1 Publication

pH dependencei

Optimum pH is 7.5. Active from pH 6 to 10.1 Publication

Pathwayi: eIF5A hypusination

This protein is involved in the pathway eIF5A hypusination, which is part of Protein modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway eIF5A hypusination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi79Iron 11
Metal bindingi80Iron 11
Metal bindingi112Iron 11
Metal bindingi113Iron 11
Metal bindingi237Iron 21
Metal bindingi238Iron 21
Metal bindingi270Iron 21
Metal bindingi271Iron 21

GO - Molecular functioni

  • deoxyhypusine monooxygenase activity Source: SGD
  • metal ion binding Source: UniProtKB-KW

GO - Biological processi

  • microtubule cytoskeleton organization Source: SGD
  • peptidyl-lysine modification to peptidyl-hypusine Source: SGD

Keywordsi

Molecular functionMonooxygenase, Oxidoreductase
Biological processHypusine biosynthesis
LigandIron, Metal-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31703-MONOMER
ReactomeiR-SCE-204626 Hypusine synthesis from eIF5A-lysine
UniPathwayiUPA00354

Names & Taxonomyi

Protein namesi
Recommended name:
Deoxyhypusine hydroxylaseUniRule annotation (EC:1.14.99.29UniRule annotation)
Short name:
DOHHUniRule annotation
Alternative name(s):
Deoxyhypusine dioxygenaseUniRule annotation
Deoxyhypusine monooxygenaseUniRule annotation
Ligand of eIF5A protein 1UniRule annotation
Gene namesi
Name:LIA1UniRule annotation
Synonyms:MMD1
Ordered Locus Names:YJR070C
ORF Names:J1814
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR070C
SGDiS000003831 LIA1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi79H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi80E → A: Abolishes enzyme activity and impairs iron-binding. 1 Publication1
Mutagenesisi112H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi113E → A: Abolishes iron-binding, substrate-binding, and enzyme activity. 1 Publication1
Mutagenesisi116E → A: Abolishes substrate-binding and enzyme activity. 1 Publication1
Mutagenesisi116E → D: Reduces enzyme activity by 97%. 1 Publication1
Mutagenesisi237H → A: Abolishes both iron-binding and enzyme activity. 1 Publication1
Mutagenesisi238E → A: Abolishes substrate-binding, enzyme activity, and impairs iron-binding. 1 Publication1
Mutagenesisi270H → A: Abolishes iron-binding, substrate-binding, and enzyme activity. 1 Publication1
Mutagenesisi271E → A: Abolishes iron-binding, substrate-binding, and enzyme activity. 1 Publication1
Mutagenesisi274E → A: Abolishes substrate-binding. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation1 Publication
ChainiPRO_00002031002 – 325Deoxyhypusine hydroxylaseAdd BLAST324

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineUniRule annotation1 Publication1
Modified residuei126PhosphoserineCombined sources1
Modified residuei187PhosphothreonineCombined sources1
Modified residuei281PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP47120
PaxDbiP47120
PRIDEiP47120

PTM databases

iPTMnetiP47120

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
HYP2P233012EBI-25526,EBI-9033

Protein-protein interaction databases

BioGridi33827, 237 interactors
DIPiDIP-2829N
IntActiP47120, 16 interactors
MINTiP47120
STRINGi4932.YJR070C

Structurei

3D structure databases

ProteinModelPortaliP47120
SMRiP47120
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati77 – 103HEAT-like PBS-type 1Add BLAST27
Repeati110 – 136HEAT-like PBS-type 2Add BLAST27
Repeati202 – 231HEAT-like PBS-type 3Add BLAST30
Repeati235 – 261HEAT-like PBS-type 4Add BLAST27
Repeati268 – 294HEAT-like PBS-type 5Add BLAST27

Sequence similaritiesi

Belongs to the deoxyhypusine hydroxylase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00500000044957
HOGENOMiHOG000248665
InParanoidiP47120
KOiK06072
OMAiPEFQYAD
OrthoDBiEOG092C3MKB

Family and domain databases

Gene3Di1.25.10.10, 2 hits
HAMAPiMF_03101 Deoxyhypusine_hydroxylase, 1 hit
InterProiView protein in InterPro
IPR011989 ARM-like
IPR016024 ARM-type_fold
IPR027517 Deoxyhypusine_hydroxylase
IPR004155 PBS_lyase_HEAT
SMARTiView protein in SMART
SM00567 EZ_HEAT, 5 hits
SUPFAMiSSF48371 SSF48371, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P47120-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTNFEKHFQ ENVDECTLEQ LRDILVNKSG KTVLANRFRA LFNLKTVAEE
60 70 80 90 100
FATKPEEAKK AIEYIAESFV NDKSELLKHE VAYVLGQTKN LDAAPTLRHV
110 120 130 140 150
MLDQNQEPMV RHEAAEALGA LGDKDSLDDL NKAAKEDPHV AVRETCELAI
160 170 180 190 200
NRINWTHGGA KDKENLQQSL YSSIDPAPPL PLEKDATIPE LQALLNDPKQ
210 220 230 240 250
PLFQRYRAMF RLRDIGTDEA ILALATGFSA ESSLFKHEIA YVFGQIGSPA
260 270 280 290 300
AVPSLIEVLG RKEEAPMVRH EAAEALGAIA SPEVVDVLKS YLNDEVDVVR
310 320
ESCIVALDMY DYENSNELEY APTAN
Length:325
Mass (Da):36,165
Last modified:February 1, 1996 - v1
Checksum:iAB36A73B40466CD5
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Z49570 Genomic DNA Translation: CAA89598.1
L47993 Genomic DNA Translation: AAB39296.1
BK006943 Genomic DNA Translation: DAA08857.1
PIRiS57089
RefSeqiNP_012604.1, NM_001181728.1

Genome annotation databases

EnsemblFungiiYJR070C; YJR070C; YJR070C
GeneIDi853534
KEGGisce:YJR070C

Similar proteinsi

Entry informationi

Entry nameiDOHH_YEAST
AccessioniPrimary (citable) accession number: P47120
Secondary accession number(s): D6VWP1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: March 28, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health