P47119 (ITPA_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inosine triphosphate pyrophosphatase
Short name=ITPase
Short name=Inosine triphosphatase
EC=3.6.1.19

Alternative name(s):
Hydroxylaminopurine sensitivity protein 1
Non-canonical purine NTP pyrophosphatase
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Name:	HAM1
Ordered Locus Names:	YJR069C
ORF Names:	J1811

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	197 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes the non-canonical purine nucleotides inosine triphosphate (ITP), deoxyinosine triphosphate (dITP) as well as 2'-deoxy-N-6-hydroxylaminopurine triposphate (dHAPTP) and 5-bromodeoxyuridine 5'-triphosphate (BrdUTP) to their respective monophosphate derivatives. Xanthosine 5'-triphosphate (XTP) is also a potential substrate. The enzyme does not distinguish between the deoxy- and ribose forms. Probably excludes non-canonical purines from RNA and DNA precursor pools, thus preventing their incorporation into RNA and DNA and avoiding chromosomal lesions. Ref.6 Ref.9 Ref.10
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate.
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm. Nucleus Ref.7.
Miscellaneous	Present with 3490 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the HAM1 NTPase family.
Biophysicochemical properties	Kinetic parameters: Vmax values are similar for dITP, dHAPTP and dGTP. KM=13.4 µM for dITP Ref.10 KM=26.9 µM for dHAPTP KM=722 µM for dGTP

Ontologies

Keywords
Biological process	Nucleotide metabolism
Cellular component	Cytoplasm Nucleus
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	deoxyribonucleoside triphosphate catabolic process Inferred from direct assay Ref.10. Source: SGD response to DNA damage stimulus Inferred from mutant phenotype Ref.1. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.7. Source: SGD nucleus Inferred from direct assay Ref.7. Source: SGD
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphate diphosphatase activity Inferred from direct assay Ref.10. Source: SGD nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 197	197	Inosine triphosphate pyrophosphatase		PRO_0000178279
Regions
	Region	10 – 15	6	Substrate binding By similarity
	Region	76 – 77	2	Substrate binding By similarity
	Region	151 – 154	4	Substrate binding By similarity
	Region	180 – 181	2	Substrate binding By similarity
Sites
	Metal binding	45	1	Magnesium or manganese By similarity
	Metal binding	76	1	Magnesium or manganese By similarity
	Binding site	58	1	Substrate By similarity
	Binding site	175	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P47119 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 1C7AA19465467C07	FASTA	197	22,093
	10 20 30 40 50 60 MSNNEIVFVT GNANKLKEVQ SILTQEVDNN NKTIHLINEA LDLEELQDTD LNAIALAKGK 70 80 90 100 110 120 QAVAALGKGK PVFVEDTALR FDEFNGLPGA YIKWFLKSMG LEKIVKMLEP FENKNAEAVT 130 140 150 160 170 180 TICFADSRGE YHFFQGITRG KIVPSRGPTT FGWDSIFEPF DSHGLTYAEM SKDAKNAISH 190 RGKAFAQFKE YLYQNDF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"HAM1, the gene controlling 6-N-hydroxylaminopurine sensitivity and mutagenesis in the yeast Saccharomyces cerevisiae." Noskov V.N., Staak K., Shcherbakova P.V., Kozmin S.G., Negishi K., Ono B.-C., Hayatsu H., Pavlov Y.I. Yeast 12:17-29(1996) [PubMed: 8789257] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed: 8840504] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Overexpression of the yeast HAM1 gene prevents 6-N-hydroxylaminopurine mutagenesis in Escherichia coli." Kozmin S.G., Leroy P., Pavlov Y.I. Acta Biochim. Pol. 45:645-652(1998) [PubMed: 9918490] [Abstract] Cited for: FUNCTION.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"Overexpression of HAM1 gene detoxifies 5-bromodeoxyuridine in the yeast Saccharomyces cerevisiae." Takayama S., Fujii M., Kurosawa A., Adachi N., Ayusawa D. Curr. Genet. 52:203-211(2007) [PubMed: 17899088] [Abstract] Cited for: FUNCTION.
[10]	"Substrate specificity of RdgB protein, a deoxyribonucleoside triphosphate pyrophosphohydrolase." Burgis N.E., Cunningham R.P. J. Biol. Chem. 282:3531-3538(2007) [PubMed: 17090528] [Abstract] Cited for: SUBSTRATE SPECIFICITY, FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49569 Genomic DNA. Translation: CAA89597.1. L47993 Genomic DNA. Translation: AAB39295.1. AY557897 Genomic DNA. Translation: AAS56223.1. BK006943 Genomic DNA. Translation: DAA08856.1.
PIR	S57088.
RefSeq	NP_012603.1. NM_001181727.1.
3D structure databases
ProteinModelPortal	P47119.
SMR	P47119. Positions 1-194.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5491N.
IntAct	P47119. 6 interactions.
MINT	MINT-476946.
STRING	P47119.
Proteomic databases
PeptideAtlas	P47119.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR069C; YJR069C; YJR069C.
GeneID	853532.
KEGG	sce:YJR069C.
NMPDR	fig|4932.3.peg.3577.
Organism-specific databases
CYGD	YJR069c.
SGD	S000003830. HAM1.
Phylogenomic databases
eggNOG	fuNOG09584.
GeneTree	EFGT00050000005897.
HOGENOM	HBG697237.
OMA	ARQVQGP.
OrthoDB	EOG4C2MM1.
Gene expression databases
ArrayExpress	P47119.
Genevestigator	P47119.
GermOnline	YJR069C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002637. Ham1p-like. [Graphical view]
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
TIGRFAMs	TIGR00042. TIGR00042. 1 hit.
ProtoNet	Search...
Other
NextBio	974233.

Entry information

Entry name

ITPA_YEAST

Accession

Primary (citable) accession number: P47119
Secondary accession number(s): D6VWP0

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program