ID   ARP3_YEAST              Reviewed;         449 AA.
AC   P47117; D6VWN6;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   27-MAR-2024, entry version 181.
DE   RecName: Full=Actin-related protein 3;
DE   AltName: Full=Actin-like protein ARP3;
DE            Short=Actin-like protein 3;
GN   Name=ARP3; Synonyms=ACT4; OrderedLocusNames=YJR065C; ORFNames=J1760;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8840501;
RX   DOI=10.1002/(sici)1097-0061(199607)12:9<839::aid-yea982>3.0.co;2-8;
RA   Huang M.-E., Souciet J.-L., Chuat J.-C., Galibert F.;
RT   "Identification of ACT4, a novel essential actin-related gene in the yeast
RT   Saccharomyces cerevisiae.";
RL   Yeast 12:839-848(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8840504;
RX   DOI=10.1002/(sici)1097-0061(199607)12:9<869::aid-yea964>3.0.co;2-1;
RA   Huang M.-E., Manus V., Chuat J.-C., Galibert F.;
RT   "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading
RT   frames and a gene cluster with a counterpart on chromosome XI.";
RL   Yeast 12:869-875(1996).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [4]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [5]
RP   IDENTIFICATION IN THE ARP2/3 COMPLEX, AND SUBCELLULAR LOCATION.
RX   PubMed=9210376; DOI=10.1016/s0960-9822(06)00223-5;
RA   Winter D., Podtelejnikov A.V., Mann M., Li R.;
RT   "The complex containing actin-related proteins Arp2 and Arp3 is required
RT   for the motility and integrity of yeast actin patches.";
RL   Curr. Biol. 7:519-529(1997).
RN   [6]
RP   ERRATUM OF PUBMED:9210376.
RA   Winter D., Podtelejnikov A.V., Mann M., Li R.;
RL   Curr. Biol. 7:R593-R593(1997).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
CC   -!- FUNCTION: Functions as ATP-binding component of the Arp2/3 complex
CC       which is involved in regulation of actin polymerization and together
CC       with an activating nucleation-promoting factor (NPF) mediates the
CC       formation of branched actin networks. Seems to contact the pointed end
CC       of the daughter actin filament (By similarity). {ECO:0000250}.
CC   -!- SUBUNIT: Component of the Arp2/3 complex composed of ARP2, ARP3,
CC       ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and
CC       ARC16/p16-ARC. {ECO:0000269|PubMed:9210376}.
CC   -!- INTERACTION:
CC       P47117; P38328: ARC40; NbExp=4; IntAct=EBI-2933, EBI-2777;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, actin patch
CC       {ECO:0000269|PubMed:9210376}.
CC   -!- MISCELLANEOUS: Present with 6650 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the actin family. ARP3 subfamily. {ECO:0000305}.
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DR   EMBL; L37111; AAC37503.1; -; Genomic_DNA.
DR   EMBL; L47993; AAB39291.1; -; Genomic_DNA.
DR   EMBL; Z49565; CAA89593.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08852.1; -; Genomic_DNA.
DR   PIR; S57084; S57084.
DR   RefSeq; NP_012599.3; NM_001181723.3.
DR   AlphaFoldDB; P47117; -.
DR   SMR; P47117; -.
DR   BioGRID; 33822; 825.
DR   ComplexPortal; CPX-607; Actin-related protein 2/3 complex.
DR   DIP; DIP-2649N; -.
DR   IntAct; P47117; 11.
DR   MINT; P47117; -.
DR   STRING; 4932.YJR065C; -.
DR   iPTMnet; P47117; -.
DR   MaxQB; P47117; -.
DR   PaxDb; 4932-YJR065C; -.
DR   PeptideAtlas; P47117; -.
DR   TopDownProteomics; P47117; -.
DR   EnsemblFungi; YJR065C_mRNA; YJR065C; YJR065C.
DR   GeneID; 853528; -.
DR   KEGG; sce:YJR065C; -.
DR   AGR; SGD:S000003826; -.
DR   SGD; S000003826; ARP3.
DR   VEuPathDB; FungiDB:YJR065C; -.
DR   eggNOG; KOG0678; Eukaryota.
DR   HOGENOM; CLU_027965_3_0_1; -.
DR   InParanoid; P47117; -.
DR   OMA; GIHYPIR; -.
DR   OrthoDB; 586415at2759; -.
DR   BioCyc; YEAST:G3O-31698-MONOMER; -.
DR   Reactome; R-SCE-2029482; Regulation of actin dynamics for phagocytic cup formation.
DR   Reactome; R-SCE-5663213; RHO GTPases Activate WASPs and WAVEs.
DR   BioGRID-ORCS; 853528; 2 hits in 10 CRISPR screens.
DR   PRO; PR:P47117; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P47117; Protein.
DR   GO; GO:0030479; C:actin cortical patch; IDA:SGD.
DR   GO; GO:0015629; C:actin cytoskeleton; NAS:ComplexPortal.
DR   GO; GO:0005885; C:Arp2/3 protein complex; IDA:SGD.
DR   GO; GO:0005886; C:plasma membrane; HDA:SGD.
DR   GO; GO:0003779; F:actin binding; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; IDA:SGD.
DR   GO; GO:0044396; P:actin cortical patch organization; IMP:SGD.
DR   GO; GO:0045010; P:actin nucleation; NAS:ComplexPortal.
DR   GO; GO:0034314; P:Arp2/3 complex-mediated actin nucleation; IMP:SGD.
DR   CDD; cd00012; NBD_sugar-kinase_HSP70_actin; 1.
DR   Gene3D; 3.30.420.40; -; 2.
DR   InterPro; IPR004000; Actin.
DR   InterPro; IPR020902; Actin/actin-like_CS.
DR   InterPro; IPR043129; ATPase_NBD.
DR   PANTHER; PTHR11937; ACTIN; 1.
DR   PANTHER; PTHR11937:SF31; ACTIN-RELATED PROTEIN 3; 1.
DR   Pfam; PF00022; Actin; 2.
DR   SMART; SM00268; ACTIN; 1.
DR   SUPFAM; SSF53067; Actin-like ATPase domain; 2.
DR   PROSITE; PS01132; ACTINS_ACT_LIKE; 1.
PE   1: Evidence at protein level;
KW   Actin-binding; ATP-binding; Cytoplasm; Cytoskeleton; Nucleotide-binding;
KW   Reference proteome.
FT   CHAIN           1..449
FT                   /note="Actin-related protein 3"
FT                   /id="PRO_0000089090"
SQ   SEQUENCE   449 AA;  49542 MW;  5BBD87E83F476E9F CRC64;
     MSYLNNPAVV MDNGTGLTKL GFAGNDSPSW VFPTAIATAA PSNTKKSSGV GAPSAVSNEA
     SYFGNSTSAT NFNGATGGLL SNNLSGKRGT EDLDFYIGNE ALVASQGPSY SLSYPIRHGQ
     VENWDHMERF WENSIFKYLR TEPEDHFFLL TEPPLNPPEN REQVAEIFFE SFNCAGLYIA
     VQAVLALAAS WTSSKVTDRS LTGTVIDSGD GVTHVIPVAE GYVIGSAIKN IPIAGRDITL
     FIQSLLRERG EADTSLRTAE KIKQEYCYVC PDIVKEFNKF DKDPSKFAQF VVENQEKTRR
     KVVDIGYERF LAPEIFFNPE IASSDFLTPL PTVVDQTIQA CPIDVRKGLY NNIVLSGGST
     MFKDFGRRLQ RDLKSIVNNR IAQSELLSGT KSTGVDVSVI SHRKQRNAVW FGGSLLAQTA
     EFKGYCHTKK DYEEYGPEIV RNFSLFNMV
//