Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Actin-related protein 3

Gene

ARP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament (By similarity).By similarity

GO - Molecular functioni

  • ATP binding Source: SGD

GO - Biological processi

  • actin filament organization Source: SGD
  • Arp2/3 complex-mediated actin nucleation Source: SGD
  • mitochondrion inheritance Source: SGD
Complete GO annotation...

Keywords - Ligandi

Actin-binding, ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31698-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Actin-related protein 3
Alternative name(s):
Actin-like protein ARP3
Short name:
Actin-like protein 3
Gene namesi
Name:ARP3
Synonyms:ACT4
Ordered Locus Names:YJR065C
ORF Names:J1760
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311 Componenti: Chromosome X

Organism-specific databases

CYGDiYJR065c.
EuPathDBiFungiDB:YJR065C.
SGDiS000003826. ARP3.

Subcellular locationi

GO - Cellular componenti

  • actin cortical patch Source: UniProtKB-SubCell
  • Arp2/3 protein complex Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 449449Actin-related protein 3PRO_0000089090Add
BLAST

Proteomic databases

MaxQBiP47117.
PaxDbiP47117.
PeptideAtlasiP47117.

Interactioni

Subunit structurei

Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
ARC40P383283EBI-2933,EBI-2777

Protein-protein interaction databases

BioGridi33822. 71 interactions.
DIPiDIP-2649N.
IntActiP47117. 10 interactions.
MINTiMINT-526200.
STRINGi4932.YJR065C.

Structurei

3D structure databases

ProteinModelPortaliP47117.
SMRiP47117. Positions 6-445.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the actin family. ARP3 subfamily.Curated

Phylogenomic databases

eggNOGiCOG5277.
GeneTreeiENSGT00550000074695.
HOGENOMiHOG000233339.
InParanoidiP47117.
KOiK18584.
OMAiQVENWDH.
OrthoDBiEOG7TJ3T6.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR015623. Arp3.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF31. PTHR11937:SF31. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47117-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSYLNNPAVV MDNGTGLTKL GFAGNDSPSW VFPTAIATAA PSNTKKSSGV
60 70 80 90 100
GAPSAVSNEA SYFGNSTSAT NFNGATGGLL SNNLSGKRGT EDLDFYIGNE
110 120 130 140 150
ALVASQGPSY SLSYPIRHGQ VENWDHMERF WENSIFKYLR TEPEDHFFLL
160 170 180 190 200
TEPPLNPPEN REQVAEIFFE SFNCAGLYIA VQAVLALAAS WTSSKVTDRS
210 220 230 240 250
LTGTVIDSGD GVTHVIPVAE GYVIGSAIKN IPIAGRDITL FIQSLLRERG
260 270 280 290 300
EADTSLRTAE KIKQEYCYVC PDIVKEFNKF DKDPSKFAQF VVENQEKTRR
310 320 330 340 350
KVVDIGYERF LAPEIFFNPE IASSDFLTPL PTVVDQTIQA CPIDVRKGLY
360 370 380 390 400
NNIVLSGGST MFKDFGRRLQ RDLKSIVNNR IAQSELLSGT KSTGVDVSVI
410 420 430 440
SHRKQRNAVW FGGSLLAQTA EFKGYCHTKK DYEEYGPEIV RNFSLFNMV
Length:449
Mass (Da):49,542
Last modified:November 1, 1995 - v1
Checksum:i5BBD87E83F476E9F
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37111 Genomic DNA. Translation: AAC37503.1.
L47993 Genomic DNA. Translation: AAB39291.1.
Z49565 Genomic DNA. Translation: CAA89593.1.
BK006943 Genomic DNA. Translation: DAA08852.1.
PIRiS57084.
RefSeqiNP_012599.3. NM_001181723.3.

Genome annotation databases

EnsemblFungiiYJR065C; YJR065C; YJR065C.
GeneIDi853528.
KEGGisce:YJR065C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L37111 Genomic DNA. Translation: AAC37503.1.
L47993 Genomic DNA. Translation: AAB39291.1.
Z49565 Genomic DNA. Translation: CAA89593.1.
BK006943 Genomic DNA. Translation: DAA08852.1.
PIRiS57084.
RefSeqiNP_012599.3. NM_001181723.3.

3D structure databases

ProteinModelPortaliP47117.
SMRiP47117. Positions 6-445.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33822. 71 interactions.
DIPiDIP-2649N.
IntActiP47117. 10 interactions.
MINTiMINT-526200.
STRINGi4932.YJR065C.

Proteomic databases

MaxQBiP47117.
PaxDbiP47117.
PeptideAtlasiP47117.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR065C; YJR065C; YJR065C.
GeneIDi853528.
KEGGisce:YJR065C.

Organism-specific databases

CYGDiYJR065c.
EuPathDBiFungiDB:YJR065C.
SGDiS000003826. ARP3.

Phylogenomic databases

eggNOGiCOG5277.
GeneTreeiENSGT00550000074695.
HOGENOMiHOG000233339.
InParanoidiP47117.
KOiK18584.
OMAiQVENWDH.
OrthoDBiEOG7TJ3T6.

Enzyme and pathway databases

BioCyciYEAST:G3O-31698-MONOMER.

Miscellaneous databases

NextBioi974221.
PROiP47117.

Family and domain databases

InterProiIPR004000. Actin.
IPR020902. Actin/actin-like_CS.
IPR015623. Arp3.
[Graphical view]
PANTHERiPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF31. PTHR11937:SF31. 1 hit.
PfamiPF00022. Actin. 1 hit.
[Graphical view]
SMARTiSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of ACT4, a novel essential actin-related gene in the yeast Saccharomyces cerevisiae."
    Huang M.-E., Souciet J.-L., Chuat J.-C., Galibert F.
    Yeast 12:839-848(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches."
    Winter D., Podtelejnikov A.V., Mann M., Li R.
    Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
  6. Erratum
    Winter D., Podtelejnikov A.V., Mann M., Li R.
    Curr. Biol. 7:R593-R593(1997)
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiARP3_YEAST
AccessioniPrimary (citable) accession number: P47117
Secondary accession number(s): D6VWN6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 24, 2015
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6650 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.