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P47117 (ARP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Actin-related protein 3
Alternative name(s):
Actin-like protein ARP3
Short name=Actin-like protein 3
Gene names
Name:ARP3
Synonyms:ACT4
Ordered Locus Names:YJR065C
ORF Names:J1760
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length449 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity.

Subunit structure

Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC. Ref.5

Subcellular location

Cytoplasmcytoskeletonactin patch Ref.5.

Miscellaneous

Present with 6650 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the actin family. ARP3 subfamily.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

ARC40P383283EBI-2933,EBI-2777

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 449449Actin-related protein 3
PRO_0000089090

Sequences

Sequence LengthMass (Da)Tools
P47117 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 5BBD87E83F476E9F

FASTA44949,542
        10         20         30         40         50         60 
MSYLNNPAVV MDNGTGLTKL GFAGNDSPSW VFPTAIATAA PSNTKKSSGV GAPSAVSNEA 

        70         80         90        100        110        120 
SYFGNSTSAT NFNGATGGLL SNNLSGKRGT EDLDFYIGNE ALVASQGPSY SLSYPIRHGQ 

       130        140        150        160        170        180 
VENWDHMERF WENSIFKYLR TEPEDHFFLL TEPPLNPPEN REQVAEIFFE SFNCAGLYIA 

       190        200        210        220        230        240 
VQAVLALAAS WTSSKVTDRS LTGTVIDSGD GVTHVIPVAE GYVIGSAIKN IPIAGRDITL 

       250        260        270        280        290        300 
FIQSLLRERG EADTSLRTAE KIKQEYCYVC PDIVKEFNKF DKDPSKFAQF VVENQEKTRR 

       310        320        330        340        350        360 
KVVDIGYERF LAPEIFFNPE IASSDFLTPL PTVVDQTIQA CPIDVRKGLY NNIVLSGGST 

       370        380        390        400        410        420 
MFKDFGRRLQ RDLKSIVNNR IAQSELLSGT KSTGVDVSVI SHRKQRNAVW FGGSLLAQTA 

       430        440 
EFKGYCHTKK DYEEYGPEIV RNFSLFNMV 

« Hide

References

« Hide 'large scale' references
[1]"Identification of ACT4, a novel essential actin-related gene in the yeast Saccharomyces cerevisiae."
Huang M.-E., Souciet J.-L., Chuat J.-C., Galibert F.
Yeast 12:839-848(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
Huang M.-E., Manus V., Chuat J.-C., Galibert F.
Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[5]"The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches."
Winter D., Podtelejnikov A.V., Mann M., Li R.
Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
[6]Erratum
Winter D., Podtelejnikov A.V., Mann M., Li R.
Curr. Biol. 7:R593-R593(1997)
[7]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L37111 Genomic DNA. Translation: AAC37503.1.
L47993 Genomic DNA. Translation: AAB39291.1.
Z49565 Genomic DNA. Translation: CAA89593.1.
BK006943 Genomic DNA. Translation: DAA08852.1.
PIRS57084.
RefSeqNP_012599.3. NM_001181723.3.

3D structure databases

ProteinModelPortalP47117.
SMRP47117. Positions 6-445.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33822. 68 interactions.
DIPDIP-2649N.
IntActP47117. 10 interactions.
MINTMINT-526200.
STRING4932.YJR065C.

Proteomic databases

MaxQBP47117.
PaxDbP47117.
PeptideAtlasP47117.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR065C; YJR065C; YJR065C.
GeneID853528.
KEGGsce:YJR065C.

Organism-specific databases

CYGDYJR065c.
SGDS000003826. ARP3.

Phylogenomic databases

eggNOGCOG5277.
GeneTreeENSGT00550000074695.
HOGENOMHOG000233339.
OMAGDPRKWI.
OrthoDBEOG7TJ3T6.

Enzyme and pathway databases

BioCycYEAST:G3O-31698-MONOMER.

Gene expression databases

GenevestigatorP47117.

Family and domain databases

InterProIPR004000. Actin-related.
IPR020902. Actin/actin-like_CS.
IPR015623. Arp3.
[Graphical view]
PANTHERPTHR11937. PTHR11937. 1 hit.
PTHR11937:SF31. PTHR11937:SF31. 1 hit.
PfamPF00022. Actin. 1 hit.
[Graphical view]
SMARTSM00268. ACTIN. 1 hit.
[Graphical view]
PROSITEPS01132. ACTINS_ACT_LIKE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio974221.
PROP47117.

Entry information

Entry nameARP3_YEAST
AccessionPrimary (citable) accession number: P47117
Secondary accession number(s): D6VWN6
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 11, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families