P47117 (ARP3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Actin-related protein 3

Alternative name(s):
Actin-like protein ARP3
Short name=Actin-like protein 3

Gene names

Name:	ARP3
Synonyms:	ACT4
Ordered Locus Names:	YJR065C
ORF Names:	J1760

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	449 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity.
Subunit structure	Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC. Ref.5
Subcellular location	Cytoplasm › cytoskeleton › actin patch Ref.5.
Miscellaneous	Present with 6650 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the actin family. ARP3 subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm Cytoskeleton
Ligand	ATP-binding Actin-binding Nucleotide-binding
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	Arp2/3 complex-mediated actin nucleation Inferred from mutant phenotype PubMed 16862144. Source: SGD mitochondrion inheritance Traceable author statement PubMed 11248049. Source: SGD
Cellular_component	Arp2/3 protein complex Inferred from direct assay PubMed 10377407. Source: SGD actin cortical patch Inferred from electronic annotation. Source: UniProtKB-SubCell plasma membrane Inferred from direct assay PubMed 16622836. Source: SGD
Molecular_function	ATP binding Inferred from mutant phenotype PubMed 15657399. Source: SGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
ARC40	P38328	5	EBI-2933,EBI-2777
ARP2	P32381	11	EBI-2933,EBI-2927

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 449	449	Actin-related protein 3		PRO_0000089090
Amino acid modifications
	Modified residue	388	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P47117 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5BBD87E83F476E9F	FASTA	449	49,542
	10 20 30 40 50 60 MSYLNNPAVV MDNGTGLTKL GFAGNDSPSW VFPTAIATAA PSNTKKSSGV GAPSAVSNEA 70 80 90 100 110 120 SYFGNSTSAT NFNGATGGLL SNNLSGKRGT EDLDFYIGNE ALVASQGPSY SLSYPIRHGQ 130 140 150 160 170 180 VENWDHMERF WENSIFKYLR TEPEDHFFLL TEPPLNPPEN REQVAEIFFE SFNCAGLYIA 190 200 210 220 230 240 VQAVLALAAS WTSSKVTDRS LTGTVIDSGD GVTHVIPVAE GYVIGSAIKN IPIAGRDITL 250 260 270 280 290 300 FIQSLLRERG EADTSLRTAE KIKQEYCYVC PDIVKEFNKF DKDPSKFAQF VVENQEKTRR 310 320 330 340 350 360 KVVDIGYERF LAPEIFFNPE IASSDFLTPL PTVVDQTIQA CPIDVRKGLY NNIVLSGGST 370 380 390 400 410 420 MFKDFGRRLQ RDLKSIVNNR IAQSELLSGT KSTGVDVSVI SHRKQRNAVW FGGSLLAQTA 430 440 EFKGYCHTKK DYEEYGPEIV RNFSLFNMV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of ACT4, a novel essential actin-related gene in the yeast Saccharomyces cerevisiae." Huang M.-E., Souciet J.-L., Chuat J.-C., Galibert F. Yeast 12:839-848(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI." Huang M.-E., Manus V., Chuat J.-C., Galibert F. Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches." Winter D., Podtelejnikov A.V., Mann M., Li R. Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
[6]	Erratum Winter D., Podtelejnikov A.V., Mann M., Li R. Curr. Biol. 7:R593-R593(1997)
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-388, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L37111 Genomic DNA. Translation: AAC37503.1. L47993 Genomic DNA. Translation: AAB39291.1. Z49565 Genomic DNA. Translation: CAA89593.1. BK006943 Genomic DNA. Translation: DAA08852.1.
PIR	S57084.
RefSeq	NP_012599.3. NM_001181723.3.
3D structure databases
ProteinModelPortal	P47117.
SMR	P47117. Positions 6-445.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2649N.
IntAct	P47117. 9 interactions.
MINT	MINT-526200.
STRING	4932.YJR065C.
Proteomic databases
PaxDb	P47117.
PeptideAtlas	P47117.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR065C; YJR065C; YJR065C.
GeneID	853528.
KEGG	sce:YJR065C. sce:YJR068W.
Organism-specific databases
CYGD	YJR065c.
SGD	S000003826. ARP3.
Phylogenomic databases
eggNOG	COG5277.
GeneTree	ENSGT00550000074695.
HOGENOM	HOG000233339.
KO	K10755.
OMA	DNVIQNC.
OrthoDB	EOG45XC4K.
Gene expression databases
Genevestigator	P47117.
GermOnline	YJR065C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR004000. Actin-related. IPR020902. Actin/actin-like_CS. IPR015623. Arp3. [Graphical view]
PANTHER	PTHR11937. PTHR11937. 1 hit. PTHR11937:SF31. PTHR11937:SF31. 1 hit.
Pfam	PF00022. Actin. 1 hit. [Graphical view]
SMART	SM00268. ACTIN. 1 hit. [Graphical view]
PROSITE	PS01132. ACTINS_ACT_LIKE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974221.

Entry information

Entry name

ARP3_YEAST

Accession

Primary (citable) accession number: P47117
Secondary accession number(s): D6VWN6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 108 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program