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P47117

- ARP3_YEAST

UniProt

P47117 - ARP3_YEAST

Protein

Actin-related protein 3

Gene

ARP3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Functions as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. Seems to contact the pointed end of the daughter actin filament By similarity.By similarity

    GO - Molecular functioni

    1. ATP binding Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. actin filament organization Source: SGD
    2. Arp2/3 complex-mediated actin nucleation Source: SGD
    3. mitochondrion inheritance Source: SGD

    Keywords - Ligandi

    Actin-binding, ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31698-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Actin-related protein 3
    Alternative name(s):
    Actin-like protein ARP3
    Short name:
    Actin-like protein 3
    Gene namesi
    Name:ARP3
    Synonyms:ACT4
    Ordered Locus Names:YJR065C
    ORF Names:J1760
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR065c.
    SGDiS000003826. ARP3.

    Subcellular locationi

    Cytoplasmcytoskeletonactin patch 1 Publication

    GO - Cellular componenti

    1. actin cortical patch Source: UniProtKB-SubCell
    2. Arp2/3 protein complex Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 449449Actin-related protein 3PRO_0000089090Add
    BLAST

    Proteomic databases

    MaxQBiP47117.
    PaxDbiP47117.
    PeptideAtlasiP47117.

    Expressioni

    Gene expression databases

    GenevestigatoriP47117.

    Interactioni

    Subunit structurei

    Component of the Arp2/3 complex composed of ARP2, ARP3, ARC40/p41-ARC, ARC35/p34-ARC, ARC18/p21-ARC, ARC19/p20-ARC and ARC16/p16-ARC.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    ARC40P383283EBI-2933,EBI-2777

    Protein-protein interaction databases

    BioGridi33822. 68 interactions.
    DIPiDIP-2649N.
    IntActiP47117. 10 interactions.
    MINTiMINT-526200.
    STRINGi4932.YJR065C.

    Structurei

    3D structure databases

    ProteinModelPortaliP47117.
    SMRiP47117. Positions 6-445.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the actin family. ARP3 subfamily.Curated

    Phylogenomic databases

    eggNOGiCOG5277.
    GeneTreeiENSGT00550000074695.
    HOGENOMiHOG000233339.
    OMAiGDPRKWI.
    OrthoDBiEOG7TJ3T6.

    Family and domain databases

    InterProiIPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR015623. Arp3.
    [Graphical view]
    PANTHERiPTHR11937. PTHR11937. 1 hit.
    PTHR11937:SF31. PTHR11937:SF31. 1 hit.
    PfamiPF00022. Actin. 1 hit.
    [Graphical view]
    SMARTiSM00268. ACTIN. 1 hit.
    [Graphical view]
    PROSITEiPS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P47117-1 [UniParc]FASTAAdd to Basket

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    MSYLNNPAVV MDNGTGLTKL GFAGNDSPSW VFPTAIATAA PSNTKKSSGV    50
    GAPSAVSNEA SYFGNSTSAT NFNGATGGLL SNNLSGKRGT EDLDFYIGNE 100
    ALVASQGPSY SLSYPIRHGQ VENWDHMERF WENSIFKYLR TEPEDHFFLL 150
    TEPPLNPPEN REQVAEIFFE SFNCAGLYIA VQAVLALAAS WTSSKVTDRS 200
    LTGTVIDSGD GVTHVIPVAE GYVIGSAIKN IPIAGRDITL FIQSLLRERG 250
    EADTSLRTAE KIKQEYCYVC PDIVKEFNKF DKDPSKFAQF VVENQEKTRR 300
    KVVDIGYERF LAPEIFFNPE IASSDFLTPL PTVVDQTIQA CPIDVRKGLY 350
    NNIVLSGGST MFKDFGRRLQ RDLKSIVNNR IAQSELLSGT KSTGVDVSVI 400
    SHRKQRNAVW FGGSLLAQTA EFKGYCHTKK DYEEYGPEIV RNFSLFNMV 449
    Length:449
    Mass (Da):49,542
    Last modified:November 1, 1995 - v1
    Checksum:i5BBD87E83F476E9F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L37111 Genomic DNA. Translation: AAC37503.1.
    L47993 Genomic DNA. Translation: AAB39291.1.
    Z49565 Genomic DNA. Translation: CAA89593.1.
    BK006943 Genomic DNA. Translation: DAA08852.1.
    PIRiS57084.
    RefSeqiNP_012599.3. NM_001181723.3.

    Genome annotation databases

    EnsemblFungiiYJR065C; YJR065C; YJR065C.
    GeneIDi853528.
    KEGGisce:YJR065C.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L37111 Genomic DNA. Translation: AAC37503.1 .
    L47993 Genomic DNA. Translation: AAB39291.1 .
    Z49565 Genomic DNA. Translation: CAA89593.1 .
    BK006943 Genomic DNA. Translation: DAA08852.1 .
    PIRi S57084.
    RefSeqi NP_012599.3. NM_001181723.3.

    3D structure databases

    ProteinModelPortali P47117.
    SMRi P47117. Positions 6-445.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33822. 68 interactions.
    DIPi DIP-2649N.
    IntActi P47117. 10 interactions.
    MINTi MINT-526200.
    STRINGi 4932.YJR065C.

    Proteomic databases

    MaxQBi P47117.
    PaxDbi P47117.
    PeptideAtlasi P47117.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR065C ; YJR065C ; YJR065C .
    GeneIDi 853528.
    KEGGi sce:YJR065C.

    Organism-specific databases

    CYGDi YJR065c.
    SGDi S000003826. ARP3.

    Phylogenomic databases

    eggNOGi COG5277.
    GeneTreei ENSGT00550000074695.
    HOGENOMi HOG000233339.
    OMAi GDPRKWI.
    OrthoDBi EOG7TJ3T6.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31698-MONOMER.

    Miscellaneous databases

    NextBioi 974221.
    PROi P47117.

    Gene expression databases

    Genevestigatori P47117.

    Family and domain databases

    InterProi IPR004000. Actin-related.
    IPR020902. Actin/actin-like_CS.
    IPR015623. Arp3.
    [Graphical view ]
    PANTHERi PTHR11937. PTHR11937. 1 hit.
    PTHR11937:SF31. PTHR11937:SF31. 1 hit.
    Pfami PF00022. Actin. 1 hit.
    [Graphical view ]
    SMARTi SM00268. ACTIN. 1 hit.
    [Graphical view ]
    PROSITEi PS01132. ACTINS_ACT_LIKE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of ACT4, a novel essential actin-related gene in the yeast Saccharomyces cerevisiae."
      Huang M.-E., Souciet J.-L., Chuat J.-C., Galibert F.
      Yeast 12:839-848(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
      Huang M.-E., Manus V., Chuat J.-C., Galibert F.
      Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    4. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    5. "The complex containing actin-related proteins Arp2 and Arp3 is required for the motility and integrity of yeast actin patches."
      Winter D., Podtelejnikov A.V., Mann M., Li R.
      Curr. Biol. 7:519-529(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION IN THE ARP2/3 COMPLEX, SUBCELLULAR LOCATION.
    6. Erratum
      Winter D., Podtelejnikov A.V., Mann M., Li R.
      Curr. Biol. 7:R593-R593(1997)
    7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiARP3_YEAST
    AccessioniPrimary (citable) accession number: P47117
    Secondary accession number(s): D6VWN6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6650 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3