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Protein

Serine/threonine-protein kinase PTK2/STK2

Gene

PTK2

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential determinant for high-affinity spermidine transport. Required for the activation of the plasma membrane proton pump PMA1 via phosphorylation of 'Ser-899'.2 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei285 – 2851ATPPROSITE-ProRule annotation
Active sitei388 – 3881Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi261 – 2699ATPPROSITE-ProRule annotation

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein kinase activity Source: SGD
  • protein serine/threonine kinase activity Source: UniProtKB-KW

GO - Biological processi

  • cellular ion homeostasis Source: SGD
  • G1/S transition of mitotic cell cycle Source: SGD
  • polyamine transport Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31692-MONOMER.
BRENDAi2.7.11.1. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PTK2/STK2 (EC:2.7.11.1)
Gene namesi
Name:PTK2
Synonyms:STK2
Ordered Locus Names:YJR059W
ORF Names:J1725
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR059W.
SGDiS000003820. PTK2.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
  • plasma membrane Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 818818Serine/threonine-protein kinase PTK2/STK2PRO_0000086591Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei56 – 561PhosphothreonineCombined sources
Modified residuei59 – 591PhosphoserineCombined sources
Modified residuei80 – 801PhosphoserineCombined sources
Modified residuei623 – 6231PhosphoserineCombined sources
Modified residuei632 – 6321PhosphoserineCombined sources
Modified residuei694 – 6941PhosphoserineCombined sources
Modified residuei700 – 7001PhosphothreonineCombined sources
Modified residuei711 – 7111PhosphoserineCombined sources
Modified residuei737 – 7371PhosphothreonineCombined sources
Modified residuei752 – 7521PhosphoserineCombined sources
Modified residuei755 – 7551PhosphoserineCombined sources
Modified residuei778 – 7781PhosphoserineCombined sources
Modified residuei781 – 7811PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47116.
PeptideAtlasiP47116.
TopDownProteomicsiP47116.

PTM databases

iPTMnetiP47116.

Interactioni

Protein-protein interaction databases

BioGridi33816. 440 interactions.
DIPiDIP-7167N.
IntActiP47116. 9 interactions.
MINTiMINT-2781034.

Structurei

3D structure databases

ProteinModelPortaliP47116.
SMRiP47116. Positions 261-607.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini255 – 562308Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00550000075309.
HOGENOMiHOG000115766.
InParanoidiP47116.
KOiK08286.
OMAiLNMIYHE.
OrthoDBiEOG79SF5K.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47116-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGNGKDKEV DKSPSVSTLK LLGKRLFNSS SHTDNSSLLL SAEQLGNGRS
60 70 80 90 100
LRKRPTSPSI SGSGSGGNSP SSSAGARQRS ASLHRRKNNA SVGFSNGSVS
110 120 130 140 150
SHKSSVALQD LIKHNNNPYL NSPSDILGTG TGIASTRDRD RAVLDREKEK
160 170 180 190 200
ERARNKERNT HHAGLPQRSN SMASHHFPNE NIVYNPYGIS PNHARPDTAF
210 220 230 240 250
ADTLNTNKEN DLSFYMHDGN SKIRMLPLPI ANPNDFLPED MKQYSVHLTD
260 270 280 290 300
NFVFDTDNKP IGSGGSSEVR KVKSSYRQKD VYALKKLNMI YHESPEKFYK
310 320 330 340 350
RCSKEFIIAK HLSHNVHITN TFYLLKVPTT TYTTRGWGFI MELGVKDLFQ
360 370 380 390 400
LMERTGWKNV PFNEKYCLFK QVAQGIKFCH DNGIAHRDLK PENVLISKEG
410 420 430 440 450
ICKLTDFGIS DWYHVIPHDY TSPVKTCQGM IGSPPYTPPE VMYFDAKKHY
460 470 480 490 500
PEKFQKPYNP LAMDSYALGI MLITMINNII PFIDSCNTDA RFREFEVSYD
510 520 530 540 550
NFINHQNPHF RDKGCHKPGP GSEYSLARNF KNTDATRIAW RLADPNPATR
560 570 580 590 600
YTMDDLFNDP FFQQIETCVE PNDDDLVRVP ELRKSTSTND FSENSLDAPH
610 620 630 640 650
DQEVIHTSNP FLKKETLTSK PRSMLEIAES PSLKQKSKVK DSAKTKTHDV
660 670 680 690 700
GDEGGNESTK PKQQDKKENL KKDEVKNGDK DKVIEEATTT NVDSILEKPT
710 720 730 740 750
PTSTKVEDNL SEDDSTMKEL KSMLNSTPTT PTHNGPTPLP AKAGTQLDKR
760 770 780 790 800
MSDLSLKSET PASTKNFSAP NVSSSSNSLR SLGSPSVSSS KKKKVIHHHL
810
DITNSVTNMS SVSAFISR
Length:818
Mass (Da):91,400
Last modified:February 1, 1996 - v1
Checksum:i55B6C882894FB4BD
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti801 – 8011D → G in AAU09754 (PubMed:17322287).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87274 Genomic DNA. Translation: BAA13325.1.
Z49559 Genomic DNA. Translation: CAA89587.1.
L47993 Genomic DNA. Translation: AAB39285.1.
AY723837 Genomic DNA. Translation: AAU09754.1.
BK006943 Genomic DNA. Translation: DAA08846.1.
PIRiS57078.
RefSeqiNP_012593.3. NM_001181717.3.

Genome annotation databases

EnsemblFungiiYJR059W; YJR059W; YJR059W.
GeneIDi853522.
KEGGisce:YJR059W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D87274 Genomic DNA. Translation: BAA13325.1.
Z49559 Genomic DNA. Translation: CAA89587.1.
L47993 Genomic DNA. Translation: AAB39285.1.
AY723837 Genomic DNA. Translation: AAU09754.1.
BK006943 Genomic DNA. Translation: DAA08846.1.
PIRiS57078.
RefSeqiNP_012593.3. NM_001181717.3.

3D structure databases

ProteinModelPortaliP47116.
SMRiP47116. Positions 261-607.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33816. 440 interactions.
DIPiDIP-7167N.
IntActiP47116. 9 interactions.
MINTiMINT-2781034.

PTM databases

iPTMnetiP47116.

Proteomic databases

MaxQBiP47116.
PeptideAtlasiP47116.
TopDownProteomicsiP47116.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR059W; YJR059W; YJR059W.
GeneIDi853522.
KEGGisce:YJR059W.

Organism-specific databases

EuPathDBiFungiDB:YJR059W.
SGDiS000003820. PTK2.

Phylogenomic databases

GeneTreeiENSGT00550000075309.
HOGENOMiHOG000115766.
InParanoidiP47116.
KOiK08286.
OMAiLNMIYHE.
OrthoDBiEOG79SF5K.

Enzyme and pathway databases

BioCyciYEAST:G3O-31692-MONOMER.
BRENDAi2.7.11.1. 984.

Miscellaneous databases

NextBioi974203.
PROiP47116.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A second gene encoding a putative serine/threonine protein kinase which enhances spermine uptake in Saccharomyces cerevisiae."
    Nozaki T., Nishimura K., Michael A.J., Maruyama T., Kakinuma Y., Igarashi K.
    Biochem. Biophys. Res. Commun. 228:452-458(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of a 62 kb DNA sequence of chromosome X reveals 36 open reading frames and a gene cluster with a counterpart on chromosome XI."
    Huang M.-E., Manus V., Chuat J.-C., Galibert F.
    Yeast 12:869-875(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  6. "The STK2 gene, which encodes a putative Ser/Thr protein kinase, is required for high-affinity spermidine transport in Saccharomyces cerevisiae."
    Kaouass M., Audette M., Ramotar D., Verma S., de Montigny D., Gamache I., Torossian K., Poulin R.
    Mol. Cell. Biol. 17:2994-3004(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  7. "Regulation of yeast H(+)-ATPase by protein kinases belonging to a family dedicated to activation of plasma membrane transporters."
    Goossens A., de La Fuente N., Forment J., Serrano R., Portillo F.
    Mol. Cell. Biol. 20:7654-7661(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-56; SER-711; THR-737; SER-752 AND SER-755, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  11. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-80, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-623; SER-711; SER-778 AND SER-781, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  13. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-56; SER-59; SER-623; SER-632; SER-694; THR-700; SER-711; SER-752 AND SER-755, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPTK2_YEAST
AccessioniPrimary (citable) accession number: P47116
Secondary accession number(s): D6VWN0, E9P962
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: May 11, 2016
This is version 149 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1420 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.