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Protein

Mitotic check point protein BFA1

Gene

BFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex.

GO - Molecular functioni

  • GDP-dissociation inhibitor activity Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • mitotic nuclear division Source: UniProtKB-KW
  • mitotic spindle orientation checkpoint Source: SGD
  • negative regulation of exit from mitosis Source: SGD
  • positive regulation of GTPase activity Source: GOC
  • regulation of Ras protein signal transduction Source: GO_Central
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31687-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic check point protein BFA1
Alternative name(s):
Cell cycle arrest protein BFA1
Gene namesi
Name:BFA1
Ordered Locus Names:YJR053W
ORF Names:J1667
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR053W.
SGDiS000003814. BFA1.

Subcellular locationi

GO - Cellular componenti

  • Bfa1-Bub2 complex Source: SGD
  • mitotic spindle pole body Source: GO_Central
  • spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000649131 – 574Mitotic check point protein BFA1Add BLAST574

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei317PhosphoserineCombined sources1

Post-translational modificationi

Multiply phosphorylated in a cell-cycle-dependent manner with the major phosphorylation occurring in mitosis.

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47113.
PRIDEiP47113.

PTM databases

iPTMnetiP47113.

Interactioni

Subunit structurei

Interacts with BUB2.

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB2P264485EBI-3586,EBI-3824
GIC1P387852EBI-3586,EBI-7575

Protein-protein interaction databases

BioGridi33807. 121 interactors.
DIPiDIP-4825N.
IntActiP47113. 12 interactors.
MINTiMINT-503660.

Structurei

3D structure databases

ProteinModelPortaliP47113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi193 – 201Poly-Glu9
Compositional biasi265 – 268Poly-Ser4

Sequence similaritiesi

To S.pombe byr4.Curated

Phylogenomic databases

HOGENOMiHOG000095200.
InParanoidiP47113.
KOiK02181.
OMAiMYEIRNM.
OrthoDBiEOG092C0TW0.

Sequencei

Sequence statusi: Complete.

P47113-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIRPLTLNG LDEPETSFEE LNTTLPRFQS HETLTLEENV PPLSTSTYIP
60 70 80 90 100
PPSSVGTSDT GTVFSNSTSA FWSNKQADDD QDMEVDQDDE FLNDFQEFQN
110 120 130 140 150
KKDDFDDAIK TNFHLRNGCR TGPFKNDIFA EEFDRKLSLE DKPRLKQPRS
160 170 180 190 200
MMELKPKRKL SNSVTSRNLR SGNSVRFKKS MPNLALVNPA IREEEEDEER
210 220 230 240 250
EREDQREFNY KIDNDTQDTI LAKFSSDDEG DFLTGFEELE GEAIDETISS
260 270 280 290 300
NDKESADHPR FLKKSSSSLP LKISPAQYDI VKHDELLTPG LHRRQRDWNT
310 320 330 340 350
QQELDSFKEK RSVRHCSNQN VQLNGPAKIK TIKQQIDHNT PMKKGSMIYN
360 370 380 390 400
PKTMKWEGNE NVLSKFSDVD TANRKALLIK NKLQRDADSK KQKYSDLQHA
410 420 430 440 450
RATSRNQKVI GNMILDEQNL RWVSVSEEEA DPFAGIPEIN LPPVGKSMKK
460 470 480 490 500
RSSSPFLRSK SQVNTPFVSN DNDGVYQSTA AQARLRKYHS MRTLNGTTET
510 520 530 540 550
PEISSTFHLS SRALEKFYHE ENRWCKKLAS WFIPRDETII SVDEETIMDE
560 570
STVNSKRKSY MYEIRNMVIN STKD
Length:574
Mass (Da):66,086
Last modified:February 1, 1996 - v1
Checksum:i1EA75D26FCF041DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88756.1.
Z49553 Genomic DNA. Translation: CAA89581.1.
BK006943 Genomic DNA. Translation: DAA08840.1.
PIRiS57072.
RefSeqiNP_012587.3. NM_001181711.3.

Genome annotation databases

EnsemblFungiiYJR053W; YJR053W; YJR053W.
GeneIDi853513.
KEGGisce:YJR053W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88756.1.
Z49553 Genomic DNA. Translation: CAA89581.1.
BK006943 Genomic DNA. Translation: DAA08840.1.
PIRiS57072.
RefSeqiNP_012587.3. NM_001181711.3.

3D structure databases

ProteinModelPortaliP47113.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33807. 121 interactors.
DIPiDIP-4825N.
IntActiP47113. 12 interactors.
MINTiMINT-503660.

PTM databases

iPTMnetiP47113.

Proteomic databases

MaxQBiP47113.
PRIDEiP47113.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR053W; YJR053W; YJR053W.
GeneIDi853513.
KEGGisce:YJR053W.

Organism-specific databases

EuPathDBiFungiDB:YJR053W.
SGDiS000003814. BFA1.

Phylogenomic databases

HOGENOMiHOG000095200.
InParanoidiP47113.
KOiK02181.
OMAiMYEIRNM.
OrthoDBiEOG092C0TW0.

Enzyme and pathway databases

BioCyciYEAST:G3O-31687-MONOMER.

Miscellaneous databases

PROiP47113.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiBFA1_YEAST
AccessioniPrimary (citable) accession number: P47113
Secondary accession number(s): D6VWM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 2, 2016
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.