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P47113

- BFA1_YEAST

UniProt

P47113 - BFA1_YEAST

Protein

Mitotic check point protein BFA1

Gene

BFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Feb 1996)
      Previous versions | rss
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    Functioni

    Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex.

    GO - Molecular functioni

    1. GTPase activator activity Source: SGD
    2. protein binding Source: IntAct

    GO - Biological processi

    1. mitotic nuclear division Source: UniProtKB-KW
    2. mitotic spindle orientation checkpoint Source: SGD
    3. positive regulation of GTPase activity Source: GOC
    4. regulation of exit from mitosis Source: SGD

    Keywords - Biological processi

    Cell cycle, Cell division, Mitosis

    Enzyme and pathway databases

    BioCyciYEAST:G3O-31687-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mitotic check point protein BFA1
    Alternative name(s):
    Cell cycle arrest protein BFA1
    Gene namesi
    Name:BFA1
    Ordered Locus Names:YJR053W
    ORF Names:J1667
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome X

    Organism-specific databases

    CYGDiYJR053w.
    SGDiS000003814. BFA1.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-KW
    2. spindle pole body Source: SGD

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 574574Mitotic check point protein BFA1PRO_0000064913Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei317 – 3171Phosphoserine1 Publication

    Post-translational modificationi

    Multiply phosphorylated in a cell-cycle-dependent manner with the major phosphorylation occurring in mitosis.1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP47113.
    PaxDbiP47113.

    Expressioni

    Gene expression databases

    GenevestigatoriP47113.

    Interactioni

    Subunit structurei

    Interacts with BUB2.

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    BUB2P264485EBI-3586,EBI-3824
    GIC1P387852EBI-3586,EBI-7575

    Protein-protein interaction databases

    BioGridi33807. 115 interactions.
    DIPiDIP-4825N.
    IntActiP47113. 12 interactions.
    MINTiMINT-503660.
    STRINGi4932.YJR053W.

    Structurei

    3D structure databases

    ProteinModelPortaliP47113.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi193 – 2019Poly-Glu
    Compositional biasi265 – 2684Poly-Ser

    Sequence similaritiesi

    To S.pombe byr4.Curated

    Phylogenomic databases

    eggNOGiNOG282265.
    HOGENOMiHOG000095200.
    KOiK02181.
    OMAiMYEIRNM.
    OrthoDBiEOG7R2BX5.

    Sequencei

    Sequence statusi: Complete.

    P47113-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSIRPLTLNG LDEPETSFEE LNTTLPRFQS HETLTLEENV PPLSTSTYIP    50
    PPSSVGTSDT GTVFSNSTSA FWSNKQADDD QDMEVDQDDE FLNDFQEFQN 100
    KKDDFDDAIK TNFHLRNGCR TGPFKNDIFA EEFDRKLSLE DKPRLKQPRS 150
    MMELKPKRKL SNSVTSRNLR SGNSVRFKKS MPNLALVNPA IREEEEDEER 200
    EREDQREFNY KIDNDTQDTI LAKFSSDDEG DFLTGFEELE GEAIDETISS 250
    NDKESADHPR FLKKSSSSLP LKISPAQYDI VKHDELLTPG LHRRQRDWNT 300
    QQELDSFKEK RSVRHCSNQN VQLNGPAKIK TIKQQIDHNT PMKKGSMIYN 350
    PKTMKWEGNE NVLSKFSDVD TANRKALLIK NKLQRDADSK KQKYSDLQHA 400
    RATSRNQKVI GNMILDEQNL RWVSVSEEEA DPFAGIPEIN LPPVGKSMKK 450
    RSSSPFLRSK SQVNTPFVSN DNDGVYQSTA AQARLRKYHS MRTLNGTTET 500
    PEISSTFHLS SRALEKFYHE ENRWCKKLAS WFIPRDETII SVDEETIMDE 550
    STVNSKRKSY MYEIRNMVIN STKD 574
    Length:574
    Mass (Da):66,086
    Last modified:February 1, 1996 - v1
    Checksum:i1EA75D26FCF041DA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36344 Genomic DNA. Translation: AAA88756.1.
    Z49553 Genomic DNA. Translation: CAA89581.1.
    BK006943 Genomic DNA. Translation: DAA08840.1.
    PIRiS57072.
    RefSeqiNP_012587.3. NM_001181711.3.

    Genome annotation databases

    EnsemblFungiiYJR053W; YJR053W; YJR053W.
    GeneIDi853513.
    KEGGisce:YJR053W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L36344 Genomic DNA. Translation: AAA88756.1 .
    Z49553 Genomic DNA. Translation: CAA89581.1 .
    BK006943 Genomic DNA. Translation: DAA08840.1 .
    PIRi S57072.
    RefSeqi NP_012587.3. NM_001181711.3.

    3D structure databases

    ProteinModelPortali P47113.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 33807. 115 interactions.
    DIPi DIP-4825N.
    IntActi P47113. 12 interactions.
    MINTi MINT-503660.
    STRINGi 4932.YJR053W.

    Proteomic databases

    MaxQBi P47113.
    PaxDbi P47113.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YJR053W ; YJR053W ; YJR053W .
    GeneIDi 853513.
    KEGGi sce:YJR053W.

    Organism-specific databases

    CYGDi YJR053w.
    SGDi S000003814. BFA1.

    Phylogenomic databases

    eggNOGi NOG282265.
    HOGENOMi HOG000095200.
    KOi K02181.
    OMAi MYEIRNM.
    OrthoDBi EOG7R2BX5.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-31687-MONOMER.

    Miscellaneous databases

    NextBioi 974179.

    Gene expression databases

    Genevestigatori P47113.

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
      Huang M.-E., Chuat J.-C., Galibert F.
      Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
      Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
      , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
      EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    3. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    4. "Bifurcation of the mitotic checkpoint pathway in budding yeast."
      Li R.
      Proc. Natl. Acad. Sci. U.S.A. 96:4989-4994(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    5. "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis."
      Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.
      J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiBFA1_YEAST
    AccessioniPrimary (citable) accession number: P47113
    Secondary accession number(s): D6VWM4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: February 1, 1996
    Last sequence update: February 1, 1996
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 1380 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    2. Yeast chromosome X
      Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

    External Data

    Dasty 3