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P47113 (BFA1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 103. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mitotic check point protein BFA1
Alternative name(s):
Cell cycle arrest protein BFA1
Gene names
Name:BFA1
Ordered Locus Names:YJR053W
ORF Names:J1667
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length574 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex.

Subunit structure

Interacts with BUB2.

Subcellular location

Cytoplasmcytoskeletonspindle pole.

Post-translational modification

Multiply phosphorylated in a cell-cycle-dependent manner with the major phosphorylation occurring in mitosis.

Miscellaneous

Present with 1380 molecules/cell in log phase SD medium.

Sequence similarities

To S.pombe byr4.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

BUB2P264485EBI-3586,EBI-3824
GIC1P387852EBI-3586,EBI-7575

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 574574Mitotic check point protein BFA1
PRO_0000064913

Regions

Compositional bias193 – 2019Poly-Glu
Compositional bias265 – 2684Poly-Ser

Amino acid modifications

Modified residue3171Phosphoserine Ref.7

Sequences

Sequence LengthMass (Da)Tools
P47113 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: 1EA75D26FCF041DA

FASTA57466,086
        10         20         30         40         50         60 
MSIRPLTLNG LDEPETSFEE LNTTLPRFQS HETLTLEENV PPLSTSTYIP PPSSVGTSDT 

        70         80         90        100        110        120 
GTVFSNSTSA FWSNKQADDD QDMEVDQDDE FLNDFQEFQN KKDDFDDAIK TNFHLRNGCR 

       130        140        150        160        170        180 
TGPFKNDIFA EEFDRKLSLE DKPRLKQPRS MMELKPKRKL SNSVTSRNLR SGNSVRFKKS 

       190        200        210        220        230        240 
MPNLALVNPA IREEEEDEER EREDQREFNY KIDNDTQDTI LAKFSSDDEG DFLTGFEELE 

       250        260        270        280        290        300 
GEAIDETISS NDKESADHPR FLKKSSSSLP LKISPAQYDI VKHDELLTPG LHRRQRDWNT 

       310        320        330        340        350        360 
QQELDSFKEK RSVRHCSNQN VQLNGPAKIK TIKQQIDHNT PMKKGSMIYN PKTMKWEGNE 

       370        380        390        400        410        420 
NVLSKFSDVD TANRKALLIK NKLQRDADSK KQKYSDLQHA RATSRNQKVI GNMILDEQNL 

       430        440        450        460        470        480 
RWVSVSEEEA DPFAGIPEIN LPPVGKSMKK RSSSPFLRSK SQVNTPFVSN DNDGVYQSTA 

       490        500        510        520        530        540 
AQARLRKYHS MRTLNGTTET PEISSTFHLS SRALEKFYHE ENRWCKKLAS WFIPRDETII 

       550        560        570 
SVDEETIMDE STVNSKRKSY MYEIRNMVIN STKD 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
Huang M.-E., Chuat J.-C., Galibert F.
Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]Saccharomyces Genome Database
Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[4]"Bifurcation of the mitotic checkpoint pathway in budding yeast."
Li R.
Proc. Natl. Acad. Sci. U.S.A. 96:4989-4994(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[5]"The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis."
Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.
J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L36344 Genomic DNA. Translation: AAA88756.1.
Z49553 Genomic DNA. Translation: CAA89581.1.
BK006943 Genomic DNA. Translation: DAA08840.1.
PIRS57072.
RefSeqNP_012587.3. NM_001181711.3.

3D structure databases

ProteinModelPortalP47113.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid33807. 113 interactions.
DIPDIP-4825N.
IntActP47113. 12 interactions.
MINTMINT-503660.
STRING4932.YJR053W.

Proteomic databases

PaxDbP47113.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYJR053W; YJR053W; YJR053W.
GeneID853513.
KEGGsce:YJR053W.

Organism-specific databases

CYGDYJR053w.
SGDS000003814. BFA1.

Phylogenomic databases

eggNOGNOG282265.
HOGENOMHOG000095200.
KOK02181.
OMAMVINSTK.
OrthoDBEOG7R2BX5.

Enzyme and pathway databases

BioCycYEAST:G3O-31687-MONOMER.

Gene expression databases

GenevestigatorP47113.

Family and domain databases

ProtoNetSearch...

Other

NextBio974179.

Entry information

Entry nameBFA1_YEAST
AccessionPrimary (citable) accession number: P47113
Secondary accession number(s): D6VWM4
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: April 16, 2014
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD