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P47113

- BFA1_YEAST

UniProt

P47113 - BFA1_YEAST

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Protein

Mitotic check point protein BFA1

Gene

BFA1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Part of a checkpoint which monitors spindle integrity and prevents premature exit from mitosis. This cell-cycle arrest depends upon inhibition of the G-protein TEM1 by the BFA1/BUB2 complex.

GO - Molecular functioni

  1. GTPase activator activity Source: SGD

GO - Biological processi

  1. mitotic nuclear division Source: UniProtKB-KW
  2. mitotic spindle orientation checkpoint Source: SGD
  3. positive regulation of GTPase activity Source: GOC
  4. regulation of exit from mitosis Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Enzyme and pathway databases

BioCyciYEAST:G3O-31687-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Mitotic check point protein BFA1
Alternative name(s):
Cell cycle arrest protein BFA1
Gene namesi
Name:BFA1
Ordered Locus Names:YJR053W
ORF Names:J1667
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome X

Organism-specific databases

CYGDiYJR053w.
SGDiS000003814. BFA1.

Subcellular locationi

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
  2. spindle pole body Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Cytoskeleton

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 574574Mitotic check point protein BFA1PRO_0000064913Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei317 – 3171Phosphoserine1 Publication

Post-translational modificationi

Multiply phosphorylated in a cell-cycle-dependent manner with the major phosphorylation occurring in mitosis.1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP47113.
PaxDbiP47113.

Expressioni

Gene expression databases

GenevestigatoriP47113.

Interactioni

Subunit structurei

Interacts with BUB2.

Binary interactionsi

WithEntry#Exp.IntActNotes
BUB2P264485EBI-3586,EBI-3824
GIC1P387852EBI-3586,EBI-7575

Protein-protein interaction databases

BioGridi33807. 115 interactions.
DIPiDIP-4825N.
IntActiP47113. 12 interactions.
MINTiMINT-503660.
STRINGi4932.YJR053W.

Structurei

3D structure databases

ProteinModelPortaliP47113.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi193 – 2019Poly-Glu
Compositional biasi265 – 2684Poly-Ser

Sequence similaritiesi

To S.pombe byr4.Curated

Phylogenomic databases

eggNOGiNOG282265.
HOGENOMiHOG000095200.
InParanoidiP47113.
KOiK02181.
OMAiMYEIRNM.
OrthoDBiEOG7R2BX5.

Sequencei

Sequence statusi: Complete.

P47113-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSIRPLTLNG LDEPETSFEE LNTTLPRFQS HETLTLEENV PPLSTSTYIP
60 70 80 90 100
PPSSVGTSDT GTVFSNSTSA FWSNKQADDD QDMEVDQDDE FLNDFQEFQN
110 120 130 140 150
KKDDFDDAIK TNFHLRNGCR TGPFKNDIFA EEFDRKLSLE DKPRLKQPRS
160 170 180 190 200
MMELKPKRKL SNSVTSRNLR SGNSVRFKKS MPNLALVNPA IREEEEDEER
210 220 230 240 250
EREDQREFNY KIDNDTQDTI LAKFSSDDEG DFLTGFEELE GEAIDETISS
260 270 280 290 300
NDKESADHPR FLKKSSSSLP LKISPAQYDI VKHDELLTPG LHRRQRDWNT
310 320 330 340 350
QQELDSFKEK RSVRHCSNQN VQLNGPAKIK TIKQQIDHNT PMKKGSMIYN
360 370 380 390 400
PKTMKWEGNE NVLSKFSDVD TANRKALLIK NKLQRDADSK KQKYSDLQHA
410 420 430 440 450
RATSRNQKVI GNMILDEQNL RWVSVSEEEA DPFAGIPEIN LPPVGKSMKK
460 470 480 490 500
RSSSPFLRSK SQVNTPFVSN DNDGVYQSTA AQARLRKYHS MRTLNGTTET
510 520 530 540 550
PEISSTFHLS SRALEKFYHE ENRWCKKLAS WFIPRDETII SVDEETIMDE
560 570
STVNSKRKSY MYEIRNMVIN STKD
Length:574
Mass (Da):66,086
Last modified:February 1, 1996 - v1
Checksum:i1EA75D26FCF041DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88756.1.
Z49553 Genomic DNA. Translation: CAA89581.1.
BK006943 Genomic DNA. Translation: DAA08840.1.
PIRiS57072.
RefSeqiNP_012587.3. NM_001181711.3.

Genome annotation databases

EnsemblFungiiYJR053W; YJR053W; YJR053W.
GeneIDi853513.
KEGGisce:YJR053W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88756.1 .
Z49553 Genomic DNA. Translation: CAA89581.1 .
BK006943 Genomic DNA. Translation: DAA08840.1 .
PIRi S57072.
RefSeqi NP_012587.3. NM_001181711.3.

3D structure databases

ProteinModelPortali P47113.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 33807. 115 interactions.
DIPi DIP-4825N.
IntActi P47113. 12 interactions.
MINTi MINT-503660.
STRINGi 4932.YJR053W.

Proteomic databases

MaxQBi P47113.
PaxDbi P47113.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YJR053W ; YJR053W ; YJR053W .
GeneIDi 853513.
KEGGi sce:YJR053W.

Organism-specific databases

CYGDi YJR053w.
SGDi S000003814. BFA1.

Phylogenomic databases

eggNOGi NOG282265.
HOGENOMi HOG000095200.
InParanoidi P47113.
KOi K02181.
OMAi MYEIRNM.
OrthoDBi EOG7R2BX5.

Enzyme and pathway databases

BioCyci YEAST:G3O-31687-MONOMER.

Miscellaneous databases

NextBioi 974179.

Gene expression databases

Genevestigatori P47113.

Family and domain databases

ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
    Huang M.-E., Chuat J.-C., Galibert F.
    Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Bifurcation of the mitotic checkpoint pathway in budding yeast."
    Li R.
    Proc. Natl. Acad. Sci. U.S.A. 96:4989-4994(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  5. "The Bub2-dependent mitotic pathway in yeast acts every cell cycle and regulates cytokinesis."
    Lee S.E., Jensen S., Frenz L.M., Johnson A.L., Fesquet D., Johnston L.H.
    J. Cell Sci. 114:2345-2354(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-317, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiBFA1_YEAST
AccessioniPrimary (citable) accession number: P47113
Secondary accession number(s): D6VWM4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 1380 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  2. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

External Data

Dasty 3