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Protein

Cell division cycle protein CDT1

Gene

TAH11

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA replication licensing factor, required for pre-replication complex assembly. Faithful duplication of the genetic material requires 'once per cell cycle' DNA replication initiation and elongation. Central to this control is the tightly regulated formation of prereplicative complexes (preRCs) at future origins of DNA replication. Required for the recruitment of the MCM2-7 helicase complex to the replication origins.7 Publications

GO - Molecular functioni

  • DNA replication origin binding Source: SGD

GO - Biological processi

  • cell division Source: UniProtKB-KW
  • double-strand break repair via break-induced replication Source: SGD
  • mitotic nuclear division Source: UniProtKB-KW
  • pre-replicative complex assembly involved in nuclear cell cycle DNA replication Source: SGD
  • regulation of DNA-dependent DNA replication initiation Source: SGD
Complete GO annotation...

Keywords - Biological processi

Cell cycle, Cell division, Mitosis

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-31681-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Cell division cycle protein CDT1
Alternative name(s):
SIC1 indispensable protein 2
Topoisomerase-A hypersensitive protein 11
Gene namesi
Name:TAH11
Synonyms:CDT1, SID2
Ordered Locus Names:YJR046W
ORF Names:J1641
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome X

Organism-specific databases

EuPathDBiFungiDB:YJR046W.
SGDiS000003807. TAH11.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Undergoes cell cycle-dependent changes in its nuclear localization. Exits the nucleus and remains in the cytoplasm during S phase through early mitosis, and re-accumulates in the nucleus around the end of mitosis.

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nuclear pre-replicative complex Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 604604Cell division cycle protein CDT1PRO_0000203095Add
BLAST

Proteomic databases

MaxQBiP47112.
PeptideAtlasiP47112.

PTM databases

iPTMnetiP47112.

Interactioni

Subunit structurei

Associates with the MCM2-7 complex. Interacts with MCM2, ORC1, ORC2 and ORC6.3 Publications

Protein-protein interaction databases

BioGridi33798. 29 interactions.
DIPiDIP-7948N.
IntActiP47112. 16 interactions.
MINTiMINT-4491652.

Structurei

3D structure databases

ProteinModelPortaliP47112.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Cdt1 family.Curated

Phylogenomic databases

InParanoidiP47112.
KOiK12414.
OMAiDEPDCLL.
OrthoDBiEOG7K6Q3R.

Family and domain databases

InterProiIPR032054. Cdt1_C.
[Graphical view]
PfamiPF16679. CDT1_C. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P47112-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGTANSRRK EVLRVPVIDL NRVSDEEQLL PVVRAILLQH DTFLLKNYAN
60 70 80 90 100
KAVLDALLAG LTTKDLPDTS QGFDANFTGT LPLEDDVWLE QYIFDTDPQL
110 120 130 140 150
RFDRKCRNES LCSIYSRLFK LGLFFAQLCV KSVVSSAELQ DCISTSHYAT
160 170 180 190 200
KLTRYFNDNG STHDGADAGA TVLPTGDDFQ YLFERDYVTF LPTGVLTIFP
210 220 230 240 250
CAKAIRYKPS TMATTDNSWV SIDEPDCLLF HTGTLLARWS QGMHTTSPLQ
260 270 280 290 300
IDPRANIVSL TIWPPLTTPI SSKGEGTIAN HLLEQQIKAF PKVAQQYYPR
310 320 330 340 350
ELSILRLQDA MKFVKELFTV CETVLSLNAL SRSTGVPPEL HVLLPQISSM
360 370 380 390 400
MKRKIVQDDI LKLLTIWSDA YVVELNSRGE LTMNLPKRDN LTTLTNKSRT
410 420 430 440 450
LAFVERAESW YQQVIASKDE IMTDVPAFKI NKRRSSSNSK TVLSSKVQTK
460 470 480 490 500
SSNANALNNS RYLANSKENF MYKEKMPDSQ ANLMDRLRER ERRSAALLSQ
510 520 530 540 550
RQKRYQQFLA MKMTQVFDIL FSLTRGQPYT ETYLSSLIVD SLQDSNNPIG
560 570 580 590 600
TKEASEILAG LQGILPMDIS VHQVDGGLKV YRWNSLDKNR FSKLLQIHKS

KQQD
Length:604
Mass (Da):68,405
Last modified:February 1, 1996 - v1
Checksum:i099992C0E3CCA726
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88748.1.
Z49546 Genomic DNA. Translation: CAA89574.1.
BK006943 Genomic DNA. Translation: DAA08833.1.
PIRiS57065.
RefSeqiNP_012580.1. NM_001181704.1.

Genome annotation databases

EnsemblFungiiYJR046W; YJR046W; YJR046W.
GeneIDi853504.
KEGGisce:YJR046W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L36344 Genomic DNA. Translation: AAA88748.1.
Z49546 Genomic DNA. Translation: CAA89574.1.
BK006943 Genomic DNA. Translation: DAA08833.1.
PIRiS57065.
RefSeqiNP_012580.1. NM_001181704.1.

3D structure databases

ProteinModelPortaliP47112.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33798. 29 interactions.
DIPiDIP-7948N.
IntActiP47112. 16 interactions.
MINTiMINT-4491652.

PTM databases

iPTMnetiP47112.

Proteomic databases

MaxQBiP47112.
PeptideAtlasiP47112.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYJR046W; YJR046W; YJR046W.
GeneIDi853504.
KEGGisce:YJR046W.

Organism-specific databases

EuPathDBiFungiDB:YJR046W.
SGDiS000003807. TAH11.

Phylogenomic databases

InParanoidiP47112.
KOiK12414.
OMAiDEPDCLL.
OrthoDBiEOG7K6Q3R.

Enzyme and pathway databases

BioCyciYEAST:G3O-31681-MONOMER.

Miscellaneous databases

PROiP47112.

Family and domain databases

InterProiIPR032054. Cdt1_C.
[Graphical view]
PfamiPF16679. CDT1_C. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
    Huang M.-E., Chuat J.-C., Galibert F.
    Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  2. "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
    Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K.
    , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
    EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  4. "Mutations in SID2, a novel gene in Saccharomyces cerevisiae, cause synthetic lethality with sic1 deletion and may cause a defect during S phase."
    Jacobson M.D., Munoz C.X., Knox K.S., Williams B.E., Lu L.L., Cross F.R., Vallen E.A.
    Genetics 159:17-33(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  5. "Identification of Tah11/Sid2 as the ortholog of the replication licensing factor Cdt1 in Saccharomyces cerevisiae."
    Devault A., Vallen E.A., Yuan T., Green S., Bensimon A., Schwob E.
    Curr. Biol. 12:689-694(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Interdependent nuclear accumulation of budding yeast Cdt1 and Mcm2-7 during G1 phase."
    Tanaka S., Diffley J.F.
    Nat. Cell Biol. 4:198-207(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION, INTERACTION WITH MCM2.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "Reconstitution of Saccharomyces cerevisiae prereplicative complex assembly in vitro."
    Kawasaki Y., Kim H.D., Kojima A., Seki T., Sugino A.
    Genes Cells 11:745-756(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, ASSOCIATION WITH THE MCM2-7 COMPLEX.
  9. "Sequential ATP hydrolysis by Cdc6 and ORC directs loading of the Mcm2-7 helicase."
    Randell J.C., Bowers J.L., Rodriguez H.K., Bell S.P.
    Mol. Cell 21:29-39(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING.
  10. "Interaction between ORC and Cdt1p of Saccharomyces cerevisiae."
    Asano T., Makise M., Takehara M., Mizushima T.
    FEMS Yeast Res. 7:1256-1262(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DNA-BINDING, INTERACTION WITH ORC1; ORC2 AND ORC6.
  11. "Orc6 is required for dynamic recruitment of Cdt1 during repeated Mcm2-7 loading."
    Chen S., de Vries M.A., Bell S.P.
    Genes Dev. 21:2897-2907(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH ORC6.
  12. "Concerted loading of Mcm2-7 double hexamers around DNA during DNA replication origin licensing."
    Remus D., Beuron F., Tolun G., Griffith J.D., Morris E.P., Diffley J.F.
    Cell 139:719-730(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY, ASSOCIATION WITH THE MCM2-7 COMPLEX, FUNCTION.

Entry informationi

Entry nameiCDT1_YEAST
AccessioniPrimary (citable) accession number: P47112
Secondary accession number(s): D6VWL7
Entry historyi
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: June 8, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 2190 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome X
    Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.