ID   VPS55_YEAST             Reviewed;         140 AA.
AC   P47111; D6VWL5;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   27-MAR-2024, entry version 160.
DE   RecName: Full=Vacuolar protein sorting-associated protein 55;
GN   Name=VPS55; OrderedLocusNames=YJR044C; ORFNames=J1637;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7668047; DOI=10.1002/yea.320110809;
RA   Huang M.-E., Chuat J.-C., Galibert F.;
RT   "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA
RT   genes and 14 new open reading frames including a gene most probably
RT   belonging to the family of ubiquitin-protein ligases.";
RL   Yeast 11:775-781(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=17322287; DOI=10.1101/gr.6037607;
RA   Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA   Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA   Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA   Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA   LaBaer J.;
RT   "Approaching a complete repository of sequence-verified protein-encoding
RT   clones for Saccharomyces cerevisiae.";
RL   Genome Res. 17:536-543(2007).
RN   [5]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=12006663; DOI=10.1091/mbc.01-12-0597;
RA   Belgareh-Touze N., Avaro S., Rouille Y., Hoflack B., Haguenauer-Tsapis R.;
RT   "Yeast Vps55p, a functional homolog of human obesity receptor gene-related
RT   protein, is involved in late endosome to vacuole trafficking.";
RL   Mol. Biol. Cell 13:1694-1708(2002).
RN   [6]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [7]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [8]
RP   TOPOLOGY [LARGE SCALE ANALYSIS].
RC   STRAIN=ATCC 208353 / W303-1A;
RX   PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA   Kim H., Melen K., Oesterberg M., von Heijne G.;
RT   "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
RN   [9]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-137, AND IDENTIFICATION BY
RP   MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=19779198; DOI=10.1126/science.1172867;
RA   Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.;
RT   "Global analysis of Cdk1 substrate phosphorylation sites provides insights
RT   into evolution.";
RL   Science 325:1682-1686(2009).
CC   -!- FUNCTION: Involved in protein transport from endosomes to the vacuole.
CC       {ECO:0000269|PubMed:12006663}.
CC   -!- INTERACTION:
CC       P47111; Q12016: VPS68; NbExp=3; IntAct=EBI-25497, EBI-29309;
CC   -!- SUBCELLULAR LOCATION: Endosome membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- MISCELLANEOUS: Present with 2340 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
CC   -!- SIMILARITY: Belongs to the OB-RGRP/VPS55 family. {ECO:0000305}.
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DR   EMBL; Z49544; CAA89572.1; -; Genomic_DNA.
DR   EMBL; L36344; AAA88746.1; -; Genomic_DNA.
DR   EMBL; AY557891; AAS56217.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08831.1; -; Genomic_DNA.
DR   PIR; S57063; S57063.
DR   RefSeq; NP_012578.3; NM_001181702.3.
DR   AlphaFoldDB; P47111; -.
DR   BioGRID; 33796; 218.
DR   ComplexPortal; CPX-1413; VPS55-VPS68 sorting complex.
DR   DIP; DIP-7667N; -.
DR   IntAct; P47111; 5.
DR   MINT; P47111; -.
DR   STRING; 4932.YJR044C; -.
DR   iPTMnet; P47111; -.
DR   PaxDb; 4932-YJR044C; -.
DR   PeptideAtlas; P47111; -.
DR   EnsemblFungi; YJR044C_mRNA; YJR044C; YJR044C.
DR   GeneID; 853502; -.
DR   KEGG; sce:YJR044C; -.
DR   AGR; SGD:S000003805; -.
DR   SGD; S000003805; VPS55.
DR   VEuPathDB; FungiDB:YJR044C; -.
DR   eggNOG; KOG2174; Eukaryota.
DR   GeneTree; ENSGT00390000006503; -.
DR   HOGENOM; CLU_134810_0_0_1; -.
DR   InParanoid; P47111; -.
DR   OMA; ICARCAN; -.
DR   OrthoDB; 8339at2759; -.
DR   BioCyc; YEAST:G3O-31679-MONOMER; -.
DR   BioGRID-ORCS; 853502; 0 hits in 10 CRISPR screens.
DR   PRO; PR:P47111; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P47111; Protein.
DR   GO; GO:0005768; C:endosome; IBA:GO_Central.
DR   GO; GO:0005770; C:late endosome; IDA:SGD.
DR   GO; GO:0034424; C:Vps55/Vps68 complex; IDA:SGD.
DR   GO; GO:0032511; P:late endosome to vacuole transport via multivesicular body sorting pathway; IMP:SGD.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   InterPro; IPR007262; Vps55/LEPROT.
DR   PANTHER; PTHR12050; LEPTIN RECEPTOR-RELATED; 1.
DR   PANTHER; PTHR12050:SF0; RH04491P; 1.
DR   Pfam; PF04133; Vps55; 1.
PE   1: Evidence at protein level;
KW   Endosome; Membrane; Phosphoprotein; Protein transport; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..140
FT                   /note="Vacuolar protein sorting-associated protein 55"
FT                   /id="PRO_0000215201"
FT   TOPO_DOM        1..11
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        12..32
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        33..37
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        38..58
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        59..76
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        77..97
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        98..107
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        108..128
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        129..140
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   MOD_RES         137
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:19779198"
SQ   SEQUENCE   140 AA;  15664 MW;  1F207E62F093FC6D CRC64;
     MMEFKVSPLT KIISLSGFLA LGFLLVILSC ALFHNYYPLF DILIFLLAPI PNTIFNAGNK
     YHTSDFMSDS SNTGQDLAHF LTGMLVTSGI ALPVVFYHCQ LIGHLSCIMC MIGGLIIYSS
     IVIFKWFFKK DFNEDDSLFG
//