P47111 (VPS55_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Vacuolar protein sorting-associated protein 55

Gene names

Name:	VPS55
Ordered Locus Names:	YJR044C
ORF Names:	J1637

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	140 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in protein transport from endosomes to the vacuole. Ref.5
Subcellular location	Endosome membrane; Multi-pass membrane protein Potential Ref.5 Ref.6.
Miscellaneous	Present with 2340 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the OB-RGRP/VPS55 family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	late endosome to vacuole transport via multivesicular body sorting pathway Inferred from mutant phenotype PubMed 18216282. Source: SGD protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Vps55/Vps68 complex Inferred from direct assay PubMed 18216282. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD late endosome Inferred from direct assay Ref.5. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 140	140	Vacuolar protein sorting-associated protein 55		PRO_0000215201
Regions
	Topological domain	1 – 11	11	Cytoplasmic Potential
	Transmembrane	12 – 32	21	Helical; Potential
	Topological domain	33 – 37	5	Lumenal Potential
	Transmembrane	38 – 58	21	Helical; Potential
	Topological domain	59 – 76	18	Cytoplasmic Potential
	Transmembrane	77 – 97	21	Helical; Potential
	Topological domain	98 – 107	10	Lumenal Potential
	Transmembrane	108 – 128	21	Helical; Potential
	Topological domain	129 – 140	12	Cytoplasmic Potential

Sequences

	Sequence		Length	Mass (Da)
	P47111 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: 1F207E62F093FC6D	FASTA	140	15,664
	10 20 30 40 50 60 MMEFKVSPLT KIISLSGFLA LGFLLVILSC ALFHNYYPLF DILIFLLAPI PNTIFNAGNK 70 80 90 100 110 120 YHTSDFMSDS SNTGQDLAHF LTGMLVTSGI ALPVVFYHCQ LIGHLSCIMC MIGGLIIYSS 130 140 IVIFKWFFKK DFNEDDSLFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Yeast Vps55p, a functional homolog of human obesity receptor gene-related protein, is involved in late endosome to vacuole trafficking." Belgareh-Touze N., Avaro S., Rouille Y., Hoflack B., Haguenauer-Tsapis R. Mol. Biol. Cell 13:1694-1708(2002) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A global topology map of the Saccharomyces cerevisiae membrane proteome." Kim H., Melen K., Oesterberg M., von Heijne G. Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: ATCC 208353 / W303-1A.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z49544 Genomic DNA. Translation: CAA89572.1. L36344 Genomic DNA. Translation: AAA88746.1. AY557891 Genomic DNA. Translation: AAS56217.1. BK006943 Genomic DNA. Translation: DAA08831.1.
PIR	S57063.
RefSeq	NP_012578.3. NM_001181702.3.
3D structure databases
ProteinModelPortal	P47111.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-7667N.
IntAct	P47111. 3 interactions.
MINT	MINT-485094.
STRING	4932.YJR044C.
Proteomic databases
PaxDb	P47111.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR044C; YJR044C; YJR044C.
GeneID	853502.
KEGG	sce:YJR044C. sce:YJR047C.
Organism-specific databases
CYGD	YJR044c.
SGD	S000003805. VPS55.
Phylogenomic databases
eggNOG	NOG116508.
GeneTree	ENSGT00390000006503.
HOGENOM	HOG000038515.
KO	K03263.
OMA	HILACAM.
OrthoDB	EOG4DZ54T.
Gene expression databases
Genevestigator	P47111.
GermOnline	YJR044C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR007262. VPS55. [Graphical view]
PANTHER	PTHR12050. PTHR12050. 1 hit.
Pfam	PF04133. Vps55. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	974148.

Entry information

Entry name

VPS55_YEAST

Accession

Primary (citable) accession number: P47111
Secondary accession number(s): D6VWL5

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program