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Reviewed, UniProtKB/Swiss-Prot P47110 (DPOD3_YEAST)

Last modified November 24, 2009. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    DNA polymerase delta subunit 3
Gene names
Name: POL32
Ordered Locus Names: YJR043C
ORF Names: J1626
OrganismSaccharomyces cerevisiae (Baker's yeast) [Complete proteome]
Taxonomic identifier4932 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length350 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is not processed.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.

Subunit structure

DNA polymerase delta is a heterotrimer of POL3, POL32 and HYS2. POL32 can form homodimers.

Subcellular location

Nucleus By similarity.

Miscellaneous

Present with 2410 molecules/cell in log phase SD medium. Ref.6

Binary interactions

With

Entry

#Exp.

IntAct

Notes

pcn1Q033921EBI-6084,EBI-768724From a different organism.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 350350DNA polymerase delta subunit 3
PRO_0000186050

Amino acid modifications

Modified residue2341Phosphoserine Ref.8
Modified residue3071Phosphothreonine Ref.7
Modified residue3131Phosphoserine Ref.8 Ref.7

Sequences

Sequence LengthMass (Da)Tools
P47110-1 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: D0B9CC52F26E20B2

FASTA35040,310
        10         20         30         40         50         60 
MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA KYNCVVICCY 

        70         80         90        100        110        120 
KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI IDQKDCLTIK NSYELKVSES 

       130        140        150        160        170        180 
SKIIERTKTL EEKSKPLVRP TARSKTTPEE TTGRKSKSKD MGLRSTALLA KMKKDRDDKE 

       190        200        210        220        230        240 
TSRQNELRKR KEENLQKINK QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS 

       250        260        270        280        290        300 
NDKDKNNDDL EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT 

       310        320        330        340        350 
KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK 

« Hide

References

« Hide 'large scale' references
[1]"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases."
Huang M.-E., Chuat J.-C., Galibert F.
Yeast 11:775-781(1995) [PubMed: 7668047] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[2]"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X."
Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. expand/collapse author list , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.
EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[3]"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae."
Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. expand/collapse author list , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J.
Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[4]"Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta."
Gerik K.J., Li X., Pautz A., Burgers P.M.
J. Biol. Chem. 273:19747-19755(1998) [PubMed: 9677405] [Abstract]
Cited for: CHARACTERIZATION.
[5]"Structure of DNA polymerase delta from Saccharomyces cerevisiae."
Johansson E., Majka J., Burgers P.M.
J. Biol. Chem. 276:43824-43828(2001) [PubMed: 11568188] [Abstract]
Cited for: COMPOSITION OF THE DNA POLYMERASE DELTA COMPLEX.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed: 14562106] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-307 AND SER-313, MASS SPECTROMETRY.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234 AND SER-313, MASS SPECTROMETRY.
+Additional computationally mapped references.

Cross-references

Sequence databases

L36344 Genomic DNA. Translation: AAA88745.1.
Z49543 Genomic DNA. Translation: CAA89571.1.
AY557918 Genomic DNA. Translation: AAS56244.1.
PIRS57062.
RefSeqNP_012577.1.

3D structure databases

ModBaseSearch...

Protein-protein interaction databases

DIPDIP:2523N.
IntActP47110. 9 interactions.
STRINGP47110.

Genome annotation databases

EnsemblYJR043C; YJR043C; YJR043C; Saccharomyces cerevisiae. [Genome view]
GeneID853500.
KEGGsce:YJR043C.
NMPDRfig|4932.3.peg.3551.

Organism-specific databases

CYGDYJR043c.
SGDS000003804. POL32.

Phylogenomic databases

HOGENOMP47110.
OMATIKNSYE
OrthoDBEOG9RV480

Gene expression databases

ArrayExpressP47110.
GenevestigatorP47110.
GermOnlineYJR043C. Saccharomyces cerevisiae.

Family and domain databases

InterProIPR019038. DNA_polymerase_subunit_Cdc27.
[Graphical view]
PfamPF09507. CDC27. 1 hit.
[Graphical view]
ProtoNetSearch...

Other Resources

NextBio974143.

Entry information

Entry nameDPOD3_YEAST
AccessionPrimary (citable) accession number: P47110
Entry history
Integrated into UniProtKB/Swiss-Prot: February 1, 1996
Last sequence update: February 1, 1996
Last modified: November 24, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectFPAP (Fungal Proteome Annotation Project)

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Binary interactions · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents