DNA polymerase delta subunit 3 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P47110 (DPOD3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified December 14, 2011. Version 97. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
DNA polymerase delta subunit 3

Gene names

Name:	POL32
Ordered Locus Names:	YJR043C
ORF Names:	J1626

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.
Subunit structure	DNA polymerase delta is a heterotrimer of POL3, POL32 and HYS2. POL32 can form homodimers.
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 2410 molecules/cell in log phase SD medium. Ref.7

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA replication, removal of RNA primer Inferred from direct assay. Source: SGD RNA-dependent DNA replication Inferred from direct assay. Source: SGD base-excision repair Traceable author statement. Source: SGD double-strand break repair via break-induced replication Inferred from mutant phenotype. Source: SGD leading strand elongation Traceable author statement. Source: SGD mismatch repair Non-traceable author statement. Source: SGD nucleotide-excision repair Traceable author statement. Source: SGD postreplication repair Traceable author statement. Source: SGD
Cellular component	delta DNA polymerase complex Traceable author statement. Source: SGD
Molecular function	DNA-directed DNA polymerase activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 350

350

DNA polymerase delta subunit 3

PRO_0000186050

Amino acid modifications

Modified residue

234

Phosphoserine Ref.9

Modified residue

307

Phosphothreonine Ref.8

Modified residue

313

Phosphoserine Ref.8 Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P47110 [UniParc].

Last modified February 1, 1996. Version 1.
Checksum: D0B9CC52F26E20B2
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FASTA

350

40,310

        10         20         30         40         50         60 
MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA KYNCVVICCY 

        70         80         90        100        110        120 
KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI IDQKDCLTIK NSYELKVSES 

       130        140        150        160        170        180 
SKIIERTKTL EEKSKPLVRP TARSKTTPEE TTGRKSKSKD MGLRSTALLA KMKKDRDDKE 

       190        200        210        220        230        240 
TSRQNELRKR KEENLQKINK QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS 

       250        260        270        280        290        300 
NDKDKNNDDL EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT 

       310        320        330        340        350 
KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed: 7668047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[5]	"Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta." Gerik K.J., Li X., Pautz A., Burgers P.M. J. Biol. Chem. 273:19747-19755(1998) [PubMed: 9677405] [Abstract] Cited for: CHARACTERIZATION.
[6]	"Structure of DNA polymerase delta from Saccharomyces cerevisiae." Johansson E., Majka J., Burgers P.M. J. Biol. Chem. 276:43824-43828(2001) [PubMed: 11568188] [Abstract] Cited for: COMPOSITION OF THE DNA POLYMERASE DELTA COMPLEX.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-307 AND SER-313, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234 AND SER-313, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L36344 Genomic DNA. Translation: AAA88745.1. Z49543 Genomic DNA. Translation: CAA89571.1. AY557918 Genomic DNA. Translation: AAS56244.1. BK006943 Genomic DNA. Translation: DAA08830.1.
PIR	S57062.
RefSeq	NP_012577.1. NM_001181701.1.
3D structure databases
ProteinModelPortal	P47110.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2523N.
IntAct	P47110. 9 interactions.
MINT	MINT-477170.
STRING	P47110.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YJR043C; YJR043C; YJR043C.
GeneID	853500.
KEGG	sce:YJR043C.
NMPDR	fig\|4932.3.peg.3551.
Organism-specific databases
CYGD	YJR043c.
SGD	S000003804. POL32.
Phylogenomic databases
eggNOG	fuNOG10897.
HOGENOM	HBG202238.
OMA	TIKNSYE.
OrthoDB	EOG4VDT81.
Gene expression databases
ArrayExpress	P47110.
Genevestigator	P47110.
GermOnline	YJR043C. Saccharomyces cerevisiae.
Family and domain databases
KO	K03504.
ProtoNet	Search...
Other
NextBio	974143.

Entry information

Entry name

DPOD3_YEAST

Accession

Primary (citable) accession number: P47110
Secondary accession number(s): D6VWL4

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome X

Yeast (Saccharomyces cerevisiae) chromosome X: entries and gene names

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents