Reviewed, UniProtKB/Swiss-Prot P47110 (DPOD3_YEAST)

Names and origin

Protein names

Recommended name:
    DNA polymerase delta subunit 3

Gene names

Name:	POL32
Ordered Locus Names:	YJR043C
ORF Names:	J1626

Organism

Saccharomyces cerevisiae (Baker's yeast) [Complete proteome]

Taxonomic identifier

4932 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	DNA polymerase delta (DNA polymerase III) participates in chromosomal DNA replication. It is required during synthesis of the leading and lagging DNA strands at the replication fork and binds at/or near replication origins and moves along DNA with the replication fork. It has 3'-5' proofreading exonuclease activity that correct errors arising during DNA replication. It is also involved in DNA synthesis during DNA repair.
Subunit structure	DNA polymerase delta is a heterotrimer of POL3, POL32 and HYS2. POL32 can form homodimers.
Subcellular location	Nucleus By similarity.
Miscellaneous	Present with 2410 molecules/cell in log phase SD medium. Ref.6

Ontologies

Keywords
Biological process	DNA replication
Cellular component	Nucleus
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA replication, removal of RNA primer Inferred from direct assay. Source: SGD RNA-dependent DNA replication Inferred from direct assay. Source: SGD base-excision repair Traceable author statement. Source: SGD double-strand break repair via break-induced replication Inferred from mutant phenotype. Source: SGD leading strand elongation Traceable author statement. Source: SGD mismatch repair Non-traceable author statement. Source: SGD nucleotide-excision repair Traceable author statement. Source: SGD postreplication repair Traceable author statement. Source: SGD
Cellular component	delta DNA polymerase complex Traceable author statement. Source: SGD
Molecular function	DNA-directed DNA polymerase activity Inferred from direct assay. Source: SGD protein binding Inferred from physical interaction. Source: IntAct
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
pcn1	Q03392	1	EBI-6084,EBI-768724	From a different organism.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 350	350	DNA polymerase delta subunit 3		PRO_0000186050
Amino acid modifications
	Modified residue	234	1	Phosphoserine Ref.8
	Modified residue	307	1	Phosphothreonine Ref.7
	Modified residue	313	1	Phosphoserine Ref.8 Ref.7

Sequences

	Sequence		Length	Mass (Da)
	P47110-1 [UniParc]. Last modified February 1, 1996. Version 1. Checksum: D0B9CC52F26E20B2	FASTA	350	40,310
	10 20 30 40 50 60 MDQKASYFIN EKLFTEVKPV LFTDLIHHLK IGPSMAKKLM FDYYKQTTNA KYNCVVICCY 70 80 90 100 110 120 KDQTIKIIHD LSNIPQQDSI IDCFIYAFNP MDSFIPYYDI IDQKDCLTIK NSYELKVSES 130 140 150 160 170 180 SKIIERTKTL EEKSKPLVRP TARSKTTPEE TTGRKSKSKD MGLRSTALLA KMKKDRDDKE 190 200 210 220 230 240 TSRQNELRKR KEENLQKINK QNPEREAQMK ELNNLFVEDD LDTEEVNGGS KPNSPKETDS 250 260 270 280 290 300 NDKDKNNDDL EDLLETTAED SLMDVPKIQQ TKPSETEHSK EPKSEEEPSS FIDEDGYIVT 310 320 330 340 350 KRPATSTPPR KPSPVVKRAL SSSKKQETPS SNKRLKKQGT LESFFKRKAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA genes and 14 new open reading frames including a gene most probably belonging to the family of ubiquitin-protein ligases." Huang M.-E., Chuat J.-C., Galibert F. Yeast 11:775-781(1995) [PubMed: 7668047] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[2]	"Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X." Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C., Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D., Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J., Heumann K. , Hilger F., Hollenberg C.P., Huang M.-E., Jacq C., Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E., Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T., Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B., Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R., Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N., To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H., von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L. EMBO J. 15:2031-2049(1996) [PubMed: 8641269] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[4]	"Characterization of the two small subunits of Saccharomyces cerevisiae DNA polymerase delta." Gerik K.J., Li X., Pautz A., Burgers P.M. J. Biol. Chem. 273:19747-19755(1998) [PubMed: 9677405] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Structure of DNA polymerase delta from Saccharomyces cerevisiae." Johansson E., Majka J., Burgers P.M. J. Biol. Chem. 276:43824-43828(2001) [PubMed: 11568188] [Abstract] Cited for: COMPOSITION OF THE DNA POLYMERASE DELTA COMPLEX.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry." Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F. Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed: 17287358] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-307 AND SER-313, MASS SPECTROMETRY.
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-234 AND SER-313, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L36344 Genomic DNA. Translation: AAA88745.1. Z49543 Genomic DNA. Translation: CAA89571.1. AY557918 Genomic DNA. Translation: AAS56244.1.
PIR	S57062.
RefSeq	NP_012577.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
DIP	DIP:2523N.
IntAct	P47110. 9 interactions.
STRING	P47110.
Genome annotation databases
Ensembl	YJR043C; YJR043C; YJR043C; Saccharomyces cerevisiae. [Genome view]
GeneID	853500.
KEGG	sce:YJR043C.
NMPDR	fig|4932.3.peg.3551.
Organism-specific databases
CYGD	YJR043c.
SGD	S000003804. POL32.
Phylogenomic databases
HOGENOM	P47110.
OMA	TIKNSYE
OrthoDB	EOG9RV480
Gene expression databases
ArrayExpress	P47110.
Genevestigator	P47110.
GermOnline	YJR043C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR019038. DNA_polymerase_subunit_Cdc27. [Graphical view]
Pfam	PF09507. CDC27. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	974143.

Entry information

Entry name

DPOD3_YEAST

Accession

Primary (citable) accession number: P47110

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 1, 1996
Last sequence update:	February 1, 1996
Last modified:	November 24, 2009
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)